Bifidobacterium spp.: in vitro Evaluation to Adhere to Human Enterocyte HT-29 Cells, in Different Colon pH

Abstract

The adhesion of two different Bifidobacterium strains was studied using HT-29 cell line as in vitro model for intestinal epithelium. All the strains tested have been used as probiotics, and most of them are used in the dairy and food industry. Bifidobacterium longum BB536 was used as positive control. Bacteria adhesion to HT-29 cell culture observed microscopically after Gram staining. Among the test strains, Bifidobacteruim longum BB536 was the most adhesion strain compared with Bifidobacterium pseudocatenulatum G4. For the pH there is significant different in the adhesion in the different part of the colon, whereas observe the best adhesion in pH 5.7 (Transverse) for the both strains