Biological Effect of Saponins Isolated from Nigella sativa (seeds) on Growth of Some Bacteria

Abstract

This study is designed for isolation and identification of some saponin compounds from nigella sativa(seeds), and to determine the inhibitory effect of these compounds were investigated on the growth of some bacteria, which include: Staphylococcus aureus, Bacillus subtilis, Salmonella typhi, Klebsiella pneumoniae, Proteus vulgaris and Pseudomonas aeruginosa and used the antibiotics (Gentamicin, Tetracycline) as control samples. The saponin compounds were further analyzed by IR and identificated by thin layer chromatography (TLC plate). The saponin compounds showed significant inhibiting effect on the all bacteria which was used in this study compared to commercial antibiotics as a control.