The effect of Helium - Neon laser on growth of disk stem calli of garlic ( Allium sativum ) and its DNA content

Abstract

Disc stem callus culture of Garlic ( Allium sativum ) were initiated on solidified MS medium supplemented with 1.0 mg / L Benzyl adenine, 0.5 mg / L Naphthalene acetic acid and 0.3 mg / L 2,4-dichlorophenoxy acetic acid. Results indicated that exposure of these undifferentiated tissue to helium - neon laser ray increased the fresh weight of these tissues. More over, deoxy ribonucleic acid ( DNA ) content also increased twice or three times when measured by spectrophotometer. The increase of DNA content in this case had many positive applications in cell and plant tissue culture.