Isolation and Identification of Campylobacter jejuni from Diarrhoel Cases of Children in Mosul City

Abstract

The study include the isolation of Campylobacter jejuni (C.jejuni) from children (aging between after birth to two years) with watery or bloody diarrhea in the period from September 2002 to February 2003. The bacterium was identified depending on succession in growth on selective medium: Preston Campylobacter blood-free medium and microscopical examination which revealed that it is gram negative pleomorphic, curved bacillus, S-shaped and gull-winged. Biochemical tests showed its positive reaction for catalase, oxidase and its ability to hydrolyse hippurate and indoxyl acetate but it was negative for urease and lipase. This bacterium can grow at 42o C while it failed to grow at 25o C and it tolerates 1% glycine while it can not grow in 1.5 % NaCl. Additionally the bacterium was sensetive to Nalidixic acid but resistant to Cephalothin.