CME The Vibrio Cholera

Abstract

Vibrios are gram negative rods bacteria that are all widely distributed in naturethe vibrose are found in marine and surface waters. They are curved aerobic rodsand are motile, possessing a polar flagellum. V.vibrios sero group 01 and relatedVibrios cause cholera cause sepsis or enteritis Vibrio cholerae The epidemiologyof cholera closely parallels the recognition of V.cholerae transmission in water andthe development of sanitary water system (1,2,6,9).V. C is a comma-shaped, curved rod 2-4 long .It is actively motile by means ofpolar flagellu on prolonged cultivation. Vibros may become straight rod thatresemble the gram-negative enteric bacteria (1,2,6,9).V.C. grow well at 37OC on many kinds of media .including defined mediacontaining mineral salt sand asparagines as sources of carbon and nitrogen V. C.produce convex, smooth round colonies that are opaque and granular in transmittedlight V.C. grows well on thiosulfate-citrate yellow colonies . V. are oxidasepositive,vibrio grow at Avery high Pi-Land are rapidly killed by acid (1,2,3,6).V.C. and related produce a heat-lebile entero toxin with molecular weight ofabout 84,000 consisting of subunits A and B .The genes for V.C. enterotoxin are onthe bacterial chromosome-cholera enterotoxin is antigenincally related to LT ofE-coli and can stimulate the production of neutralizing antibodies .However, theprecise role of antitoxic and antibodies in protection against cholera is not clear.(3,6)Its structure was determined with one and two dimensional NMR spectra(COSY, HMBC, HMQC, HOHAHA on nuclei 1H, 13C and 31P) and was furtherconfirmed with HPLC (18).