Flow Injection Spectrophotometric Determination of Copper in the environmental and biological samples using iodine-starch complex as chromogenic agent

Abstract

Abstract A simple and facile flow injection spectrophotometric method was developed for the determination of copper (II) in aqueous and biological samples. The method is based on the oxidation – reduction reaction between copper (II) and iodide ion in acidic medium to form iodine, the released iodine reacts with soluble starch solution to form iodine starch blue complex which is detected at 600 nm wavelength .Chemical and physical parameters were investigated. The calibration curve obeyed Beer's law between ( 1.0– 25 µg.ml-1) with a limit of detection 0.6 µg.ml-1.The relative standard deviation was 0.62% for 10.0 µg.ml-1 copper solution (n=8) with sampling rate of 35 samples / h