Activities of Some Purine Metabolic Enzymes in Sera and Tissues Homogenates of patients with Different Cervix and Uterine Diseases

Abstract

Sone aspects of purine metabolism including the activities of some enzymes involved iD purinenucleotides metabolisn: Adenosine deaminase (ADA), AMP- deaminase (AMPDA), 5'-Nocleotidase (5'-NT) and xanthiDe oxidase (XO) were srudied in human sera and tissues ofhealthy women (!$ed as nornralcontrol) and palients with different diseases as follow: Chronic cervicities and uterine sdenomyosis (wlichlvere used as pathological conlrols),benign uterine 1 mors, Prcmal;gnancy group (which represerrr parienEwith chronic cewicilies with mild lo moderate hyperplasia), a d ceryix and utefus cancer.Seveml connnon diflerences in the obtained data, which expressed ir felatioD to prolein contenl.were evident anrong the above groups. The rcsults indicalc tlat thefe were a sigDificant decrease irL sera,ADA and AMPDA specific activiiies of patienls with cervix and terus cancer conpafed with thal ofcontrol, pathological conffol (nalnely: chrooic cervicitis and uterine adenornyosis). benign uterine tLmo,,and premalignancy group(P<0.001). while there were significant increases in ADA and AMPDA specjficactivilies oftissue homogenates ofpalients with cewix and uterus cancer when compared wilh thal of thesanc above meniioned groups (P<0.0J).As fhr as 5'-NT specific activilies was concened, significant increases were obseNed in sera andLissues homogenales ofpatients with cervix and uterus cancer as compared to the othef groups (P <0.01).The dehydrogeuase activities of XO in sem and tissues homogeDates of patients wilh cervix anduterus cancef show a significant decfcase (P <0.05) in comparison to thai ofother gfoups, while thcre wereno derectable oxidase activities ofXo in sefa ofcontrol ard patients with uterus caDce