Bacterial Contamination of Imported Bulls Frozen Semen

Abstract

One hundred frozen semen straws (0.5 ml), have been taken from imported bulls, the frozen semen examination as well as evaluation in addition to bacteriological evaluation whether counting, isolation and antibiotic sensitivity, the results in current study showed all bacterial isolates were identified by using different biochemical tests and API–20 E system that used later to confirm identification, all isolated bacteria found in imported frozen semen were gram negative.45 bacterial isolates in imported frozen semen distributed in 42 isolates Stenotrophomonas maltophilia and 3 isolates Pseudomonas aeroginosa, antibiotic susceptibility test for isolated bacteria by using 14 single antibiotic discks of commonly used drugs, most isolated Steno. maltophilia was susceptible to Ceftazidime, Ciprofloxacin, Cefotaxime, Gentamicin, Amikacin, Vancomycin, Erythromycin, Chloramphenicol and Tetracyclin. The results of current study showed individual movement 61.3±2.00, viability of sperm 76.2±2.06, percentage of dead sperms 23.8±2.06, and abnormal sperms 13.54±0.67.