THE EFFECT OF INHIBITORS ON PURE UREASE ISOLATED FROM LOCAL ISOLATE PROTEUS VULGARIS

Abstract

ABSTRACTExamination the urease activity which purified from Proteus vulgaris in the presenceof six inhibitors were thiourea, hydroxy urea, EDTA, ß-Mercaptoethanol, flurofamideand Para - Benzoquinon has been observed that all these compounds had been ureaseinhibitors, results showed that all of them where competitive inhibitors exceptp- benzoqinon non-competitive. The mechanism inhibition were studying of allinhibitors compounds, there ß-Mercaptoethanol has regained enzyme effectively withthe p - benzoquinon, which indicates the existence of a sulfhydryl group (SH) on-siteeffective in not affected by the act of each thiourea and hydroxurea and the EDTA,either when using ions nickel, all inhibitors may affected by their work, exceptß -mercaptoethanol and p-benzoqinon and not recovery the enzyme activity, whichindicates that the binding site inhibitors is effective, and the results of the currentstudy appeared that the inhibitor flurofamide was the most effective inhibitors of theurease.