VACCINE PRODUCTION AGAINST Trichophyton Verrucosum ISOLATED FROM MAN AND CATTLES

Abstract

ABSTRUCTThis study consist of two parts, the first part includes isolation and diagnosis ofdermatophytes from infected human and animals. Ninety two samples werecollected from young and adult males and females patients The percentage offungal infections from human dermatological cases was 59.78%.Skin scraping and infected hair samples were obtained from 106 infectedanimals (cattles and calves) with varying ages and sex, It appeared that 44.33% ofcases were infected with ringworm.After exact diagnosis of these samples, T. verrucosum was one of dematophytesthat could be isolated from human and animal, and since it represent the majorcause of ringworm in cattle with increased proportion of transmission to human,the second stage of this study was aimed by production of vaccine againstdermatophytes to decrease the number of cases in animals and then in human.The vaccine was prepared by production of soluble antigens from sonication ofveruilent strain of fungus and then inoculated in guinea pigs by using specialvaccination program, and after administration of challenge dose they showedresistance at 100%.The whole cell extract of T. verrucosum was used in used in separation processby Gel Chromatography technique with using Sephadex G 100 for separation ofit’s protein according to molecular weight, so 2 peaks were observed which used ininduction of cell mediated immunity in sensitized animals and for identification ofhumeral immunity by using passive heamagghitination test to evaluate of efficacy.So the 2’nd peak was found to be possess Biological properties and antigenicability to induce cellular immune response and serological test, to demonstratehumeral immune.