MicroRNA 210 expression profile from human placentas of preeclamptic

Abstract

Background: Pre-eclampsia (PE) is a common pregnancy specificsyndrome that is characterized by hypertension and proteinuria.Evidence shows that pre-eclampsia is associated with alterations inplacental microRNA expression. Aims: The purpose of this study was tocompare the expression of the microRNA 210 in placentas from preeclampticpregnancies versus normal placentas.Patients and Methods: The study was carried out in the department ofGynecology and Obstetrics at Al-Yarmouk Teaching Hospital for theperiod from December 2009 to December 2010. Hundred pregnantwomen of age ranging from fifteen to forty years old, their gestationalage ranging between twenty nine to forty weeks were divided into threegroups: first group: mild Pre-eclampsia (twenty), second group: severepre-eclampsia (thirty) & third group (fifty) normotensive pregnantwomen of comparable gestation were taken as a control . After deliverythe Placentas from all patients (the study and the control groups) wereobtained and studied for the microRNA 210 expression which wasassessed by microRNA microarray and real-time reverse transcriptasepolymerasechain reaction analysis.Results: MicroRNA 210 was over expressed in the placentas of preeclampticpregnancies compared with normal placentas. In mild preeclampsiagroup the mean micro RNA-210 activity was 1.68±0.25, insevere pre-eclampsia group the mean microRNA-210 activity was1.89±0.13 and for the control group the mean microRNA-210 activity was0.99±015. There was highly statistical significance in microRNA-210activity of the studied groups since the P value for the mild preeclampsiaversus the control was 0.0001. For the severe pre-eclampsiaversus the control it was 0.0001 and for the severe pre-eclampsia versusmild pre-eclampsia was 0.0001.Conclusions: The results showed that microRNA 210 was over expressedin pre-eclamptic pregnancies (mild and severe), which suggests itsinvolvement in the pathogenesis of pre-eclampsia.