An evaluation of three fissure sealants microleakage with presence or absence of bonding agent through time intervals (In vitro study)

Abstract

Background: Pit and fissure sealant have been considered an outstanding adjunct to oral health care in thedecrease of occlusal caries onset and low progression. The aims of this in vitro study were to evaluate the marginalmicroleakage of three different types of fissure sealants (SDI, Tg and tetric N-flow) by time interval, one day and 45days, in the presence or absence of bonding agent among maxillary and mandibular teeth.Materials and methods: Seventy two sound human maxillary and mandibular first premolar teeth were collectedwhich were free from obvious carious lesions. The teeth were randomly divided into two main equal groups, group (1)and group (2), each group consists of (36) teeth involving equal numbers of maxillary and mandibular teeth. The firstgroup incubated for one day, the second incubated for (45) days. Each group divided into two subgroup; one ofthem treated with bonding agent while the other without. Then each subgroup was treated with three differentmaterials which were; Tg sealant (without fluoride) group (A), SDI sealant (containing fluoride) group (B) and Tetric Nflow(flowable composite) group (C). Each one consist of six teeth involving three maxillary and three mandibular.Then dye penetration tested by using methylene blue dye, then the teeth cleaned and sectioned by sectioningdevice and tested under microscope.Results, the results had shown that, group (C+) in both incubation periods have no microleakage (score 0), but therewas an opposite effect when using bonding agent with sealant materials not containing filler particles that showed asignificant increase in the microleakage rate as shown in groups (A+ and B+). The opposite effect was seen alsowhen used sealant materials containing filler particles but without bonding agent that seen in group (C) during bothincubation periods that showed significant increasing in microleakage rate. While the effect of fluoride was veryclear in decreasing significantly the microleakage rate after (45) days of incubation in both groups that treated withand without bonding agent (groups B and B+). Concerning the anatomical variation, there were no significantchanges in most groups regarding the microleakage rate.Conclusions: Results had shown that the microleakage can be prevented by using of flowable composite containingnanofillers that treated with bonding agent after etching of enamel with 35% phosphoric acid gel