ASSOCIATION OF CELIAC DISEASE WITH HLA-DRB1 AND HLA-DQB1 ALLELES IN A SAMPLE OF IRAQI PATIENTS

Abstract

Celiac disease (CD) is a complex disorder triggered by gluten affecting genetically predisposed individuals. The CD is triggered by the binding of one or more gliadin peptides to CD associated HLA class II molecules. Fifty patients with CD and fifty control group were studied. The sera were qualitativelymeasured for anti-TTG-IgA,IgG antibodies and anti-gliadin- IgA,IgG antibodies by ELISA method. The HLA class II (DRB1, DQB1) were genotyped by using Polymerase Chain Reaction-Sequence Specific Primers (PCR-SSP). In the current studypositivity for Anti-TTG antibodies showed a frequency of 38% in CD patients as compared with the control group 0.0%, while high frequency of Anti- gliadin antibodies positivity in celiac disease patient's sera showed 22% as compared with the control group 0.0%with a highlysignificant difference were highly (P=0.001).Human leukocyte antigen genotyping revealed that the DR-alleles, DRB1*03(01,06,08,10), DRB1*0701 and DQB1*02 (01,02) showed highly significant increased frequency in CD as compared withthe controls, while the DRB1*1302 and DQB1*0601 alleles showed significant decreased frequency in CD when compared with the control groups.