Antimicrobial activity of black seed oil & water extracts On multidrug resistant Pseudomonas aeroginosa

Abstract

Pseudomonas aeruginosa is most common cause of nosocomial infections in burn centers. This opportunistic and multidrug resistant bacterium causes severe problems for hospitalized burn patients. Samples were collected from patients attending Burn& Plastic Surgery Hospital in Duhok / Iraq, between September 2011 and March 2012, for preliminary identification of P. aeroginosa using conventional methods, then confirmed with the use of BD -PhoenixTM Automated Microbiology System. Isolates were considered Multi Drug Resistant Pseudomonas aeroginosa (MDRPa) if they showed resistance to the three or more classes of antipseudomonal agents. Accordingly thirty isolates were found MDRPa. The pattern of resistance revealed that the highest resistance was for Gentamicin 96.7%, Cefepime 96.7%, Ceftazidime 90% and Aztreonam 83.3%,while lowest resistance was detected against Amikacin 36.7% and Colistin 3.3% .The results of disc diffusion assay demonstrated that antibacterial activities of black seed oil acetone extract at 1:1,1:10,1:25 concentrations were found effective against all tested MDRPa isolates, which were statistically significant (P < 0.05),while at highest dilution 1:75 . All MDRPa isolates showed lowest activity of oil extract . The antimicrobial effects of water extract showed lowest and poor activity against all tested bacteria.