Labelling Study of Sodium Citrate With Tc99m and The Bological Behaviour Of The Labelled Complex as Kidney Functioal Agent

Abstract

Abstract:The objective of this study is to optimize the parameters for the development of aprocedure for preparing Sn-sodium citrate label with Tc99m, of providing a stable complexafter mixing with Tc99m-pertechnetate and to find the potential of technetium- 99m (Tc99m)citrate complex as a new kidney functional agent. The labeling conditions of Tc99m citratecomplex using stannous chloride dehydrate as a reducing agent for pertechnetate have beendescribed. The gel chromatography scanning (GCS) method reveals that the labelingefficiency of Tc99m-citrate complex is promoted by raising the pH of the preparation to(pH=4) using 1N NaOH. The optimal amounts of the reactants in the preparation to obtainlabeled and stable complex with high kidney uptake were found to be (1mg) sodium citrateand (100 μg) SnCl2.2H2O. The results show that high labeling yield (≥95%) for the labeledcomplex. The data of bio distribution experiments in the laboratory animals (mice), clear highradioactivity accumulation of labeled complex in kidney at 5 minutes post injection. Theresults of bio distribution of labeled complex have shown a good biological behavior withlow radioactivity accumulation in the non-target organs (blood, liver and other organs).