تنقيــة جزئية وتوصـيف انزيـم البروتيـز المستخلص من بذور نبات الفاصوليا المنبتة Phaseolus Vulgaris L. Cv. White . Partial Purification And Characterization Of Protease Extracted From Germinated Seed Of Phaseolus Vulgaris L. Cv. White.

Abstract

The protease extracted from Phaseolus vulgaris L. cv. White. was purified by several steps, including precipitation with ammonium sulfate 70-90% , ion exchange chromatography using DEAE-cellulose and gel filtration on sephacryl S-200 Colum. The obtained two proteases form (A,C). The two proteases form were characterization. The result showed purification fold and recovery (4.09, 88.49) and (8.352, 22.58) respectively. The Km and Vmax for the two forms (Aand C) were (2.5, 0.714)mM and (0.33, 0.9) mM/min respectively. The optimum pH for two form (Aand C) were (8, 5.8) respectively, and stable in the pH range of (5-6.5), (7-8.5) respectively. The two proteases form (A,C) showed maximum activity(37, 42)C respectively, and showed heat stability at (20-50)C and (20-60)C respectively.