Evaluation the efficacy of volvac® vaccine to Infectious Bronchitis and determine the best rout of administration

Abstract

Due to Infectious Bronchitis (IB) disease outbreak in Iraq this study was carried out to evaluate the viability of volvac® IB+ND MLV as mixed vaccine (Newcastle serotype lasota +infectious bronchitis mass ) which is considered the most common vaccine for IB disease in Iraq and also to determine the most benefit route of administration .This study achieved by using 96 chicks and divided into main 3 groups, each group contain 32 birds, tow groups vaccinated with volvac® IB+ND MLV vaccine and the third group is control (-) ,the first tow groups subdivided in to three sub group each subgroup contain 8 chicks vaccinated with different route .First subgroup(A) vaccinated with dropping route , Second subgroup(B) by spray route and Third subgroup(C) by manual drinking. Antibody of humoral immunity was estimated by using ELISA technique that reveled the vaccine (Newcastle serotype lasota + infectious bronchitis mass ) is benefit for Newcastle disease rather than for Infectious bronchitis , the antibody titer is higher for Newcastle disease than for Infectious bronchitis, and the most useful route is spray.