STUDY THE ANALGESIC AND SEDATIVE EFFECT OF Ocimum basilicum ALCOHOLIC EXTRACT IN MALE RATS.

Abstract

This study was carried out on male rats to evaluate the analgesic andsedative effect of different doses of Alcoholic Extract of Ocimum basilicumleaves. The analgesic effect studied by using formalin test, 20 male rats dividedto a four groups (5 each), first group exposed water orally only before injectionof formalin, second group exposed orally with Diclofenac at a dose 0.71mg/kgB.W before injection of formalin, Third and fourth groups orally incubated withAlcoholic Extract of Ocimum basilicum leaves at a dose (50 and 100mg/kg BW)respectively. While the sedative effect studied by using pentobarbitone sleepingtime test and open field test, in each test 15 male rats used and divided to a threegroups one of them treated with distillated water and the other two groupstreated with Alcoholic Extract of Ocimum basilicum leaves at a dose (50 and100mg/kg BW) respectively. Results of formalin test showed a significantreduction in the mean value of nociceptive response in Diclofenac groupspecially at late phase while, groups treated with Alcoholic Extract of Ocimumbasilicum leaves revealed a significant reduction in the nociceptive responsevalue at the both phases (early and late phase) moreover the group treated with100mg/kg showed the highest attenuation in the mean value of nociceptiveresponse compared to 50mg/ kg treated group. Furthermore, the dose 100mg/kgproduced the potent sedative effect in pentobarbitone sleeping time test andopen field test. These results pointed to analgesic and sedative effect of Ocimumbasilicum may duo to its consistence of the active analgesic and sedativecompounds and its effect are in a dose dependent manner.