The Antimicrobial Effect of Alcoholic Extract of Olive Leaves as a Root Canal Irrigant.

Abstract

Aims: The study aims to evaluate the antimicrobial effect of different concentrations of alcoholic
extract of Olive Leaves solution (0.1%, 0.2%, 0.4%, 0.6%, 0.8%) in an in vitro.It also to determines
the time required for the most effective concentration of Olive Leaves to start the effect in selected
microorganisms, and to evaluate the antimicrobial effect of most effective concentrations of alcoholic
extract of Olive Leaves solution in an in vivo study as a root canal irrigant. Materials and Methods:
The antimicrobial effect of Olive Leaves (0.1%, 0.2%, 0.4%, 0.6%, 0.8%), 2.5% sodium hypochlorite,
and normal saline were determined in vitro by using broth microdilution method. The direct exposure
test was used to evaluate the time required for 0.8% of Olive Leaves to start their antimicrobial effect
on the selected microorganisms. In an in vivo study, 36 uniradicular teeth with necrotic pulps were
chosen. The patients were divided randomly into three groups, 12 patients for each group as in the
following: Group I: alcoholic extract of Olive Leaves at 0.8%. Group II: sodium hypochlorite solution
at 2.5% as a positive control. Group III: normal saline as a negative control. Bacteriological samples
were obtained from the canal at the beginning of the first appointment; at the beginning of the second
appointment; at the end of the second appointment; at the beginning of the third appointment using
sterile wet paper point. Results: The Results showed that Olive Leaves at (0.2%, 0.4%, 0.6%, 0.8%),
and sodium hypochlorite had a significant antimicrobial effect against aerobic and anaerobic bacteria
recovered from teeth with necrotic pulps. While the normal saline and 0.1% Olive Leaves had no
significant antimicrobial effect. As for Olive Leaves exract. The best antimicrobial effect was noticed
at 0.8%, which showed a significant difference from other concentrations of Olive Leaves,but no
significant difference from that of sodium hypochlorite. The results of this test showed that 2.5%
sodium hypochlorite and 0.8% Olive Leaves had immediate effect on all selected microorganisms. The
results revealed that 0.8% alcoholic extract of Olive Leaves solution had a significant antimicrobial
effect when utilized clinically as an endodontic irrigant, which was not significantly differ from sodium
hypochlorite but significantly different from normal saline. Conclusions: Olive Leaves alcoholic
extract solution at 0.8% was an effective antimicrobial agent when used as an irrigant in endodontic
treatment of teeth with necrotic pulps.