Study on some metabolites and Ionic of ovarian follicular fluid in relation to follicular size in local female goats

Abstract

The aim of this study was to estimate some metabolites and Ionic composition of ovarian follicular fluid (FF) and It's relationship with changes of follicular size in local female goats. Ovaries were collected (200 ovaries) from 100 non-pregnant adult female goats (3-6 yr - old) slaughtered at abattoir of province of Karbala during the period from January to July 2013. The ovaries were transported to the laboratory within 2 hrs post slaughter. FF was aspirated from small (<4mm), medium (4-6mm) and (>6mm) follicles and stored at -4°C for further analysis. The FF samples were analyzed for metabolites (total protein, glucose and cholesterol) and ions (sodium, potassium and magnesium), using commercial kits. The results revealed that the mean of total protein concentration was significantly lower (P<0.05) with increased of follicular size. The mean of glucose and cholesterol concentration were significantly higher (P<0.05) with increased of follicular size. The mean of sodium concentration was significantly higher with increase of follicular size. The mean of potassium concentration was significantly lower with changes of follicular size. The differences in the mean of magnesium concentration was non-significantly between small and medium follicles, while was significantly between large follicles and both small and medium categories of follicles.