Isolation and Study some types of Bacteria which forms Biofilm for some cases of Burns

Abstract

25 samples (skin swap) from burned male and female (4-25 years old) have been collected from AL-Hussein Hospital and studied well. Samples isolation and diagnoses have been achieved on two groups (Bacteria and Yeast). Two groups of bacteria have been tested depending on the negative and positive gram stain. The results showed that the aerobic bacteria was existed because of the contamination and unsterilization sensitive test for bacteria against some antibiotics has been made. Diffusion method on agar using Muller Hinton solid media has been used. Most of negative and positive gram bacteria showed sensitivity against Ciprofloxacin while it showed resistance against Trimethoprime, this is because of the capability of the bacteria to form a biofilm that enable it to make a mutation and enzymes production have ability to destroy the antibiotics. Finally, Test tube technique has been used for all samples. The result was that 7 bacteria samples have a strong ability to produce biofilm and 5 bacteria samples have moderate ability to produce biofilm while 4 of them have week ability to produce biofilm and there were 4 samples have no ability to produce the biofilm.