Separation and Determination of Poly Aromatic Hydrocarbons in Vegetables Samples in Baghdad City Using HPLC Technique

Abstract

The Reversed Phase High Performance Liquid Chromatography (RP-HPLC) has been used for the separation of Poly aromatic hydrocarbons(PAHs) by using column Reprosil 100 C 18 which was found to be a suitable one for this purpose.The result showed that using mobile phase of (Acetonitrile-water) Reversed Phase HPLC , flow rate of (1.2 ml/min) , column temperature (30C˚) and wave length of (254nm), give a complete separation with a good resolution . The total separation time was less than 20 min.The result of the study showed that the vegetables of Baghdad city were polluted by poly aromatic hydrocarbons(PAHs) in different places where samples taken because of drainage of the heavy water ,industrial trash and trash of oil colanders.-Naphthalene, Acenaphthylene, Acenaphthene, Fluorene, Phenanthrene, Anthracene, Fluoranthene, Benzo(g,h,i)perylene , Pyrene , Benzo(a)anthracene, chryseneBenzo(a)Pyrene,Benzo(b)fluoranthene, Benzo(k)Fluoranthen , Dibenzo(a,h)Anthracene, and Indeno(1,2,3-cd)pyrene .