Table of content

Iraqi Journal of Cancer and Medical Genetics

المجلة العراقية للسرطان والوراثة الطبية

ISSN: 20786123
Publisher: Al-Mustansyriah University
Faculty: Medicine
Language: Arabic and English

This journal is Open Access

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iraqi national specialized and peer reviewed scientific journal in cancer and medical genetics published by iraqi centre for cancer and medical genetics research/ al mustansria university

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TEL: 07811187220
Email: icjmg@iccmgr.org

Table of content: 2017 volume:10 issue:2

Article
Growth inhibition and apoptosis induction in two types of women cancer cell lines using laser ablated silver nanoparticles in polyvinylpyrrolidone solution
استخدام جسيمات الفضة النانوية المصنعة بطريقة الاستئصال النبضي الليزري في في تثبيط النمو وحث الموت المبرمج في نوعين من polyvinylpyrrolidone محلول خطوط الخلايا السرطانية النسائية

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Abstract

Silver nanoparticles (AgNPs) coated with polyvinylpyrrolidone (PVP) was prepared by pulsed laser ablation method in PVP solution, 1000 pluse was used with laser energy of 800 mJ/pulse. Formation of AgNPs-PVP was confirmed by UVvisible spectrophotometer detecting surface plasmon resonance chang in the abilation solution. Concentration of AgNPs, size distribution, and surface charge of the ablated AgNPs in the produced solution was determined using atomic absorbtion spectroscopy and zeta potential analysis respectivly. Nanoparticles shape was determined using transmission electron microscope imaging. Ability of the senthezied AgNPs to inhibit the growth of cancer cells was compared to that of normal fibroblast cells using two cancer cell lines (HeLa and SKOV-3) in different concentrations (0.78, 1.56, 3.125, 6.25, 12.5 and 25μg/ml). The apoptosis events were also detected in both types of cancer cell lines using mitochondrial membrane permeability, nuclear morphology, and genomic DNA fragmentation. The contribution of AgNPs to the levels of glutathione s-transferase was also determined. According to the results, AgNPs size was 28.43nm with spherical shape and peak UV-Vis absorbance ranged between 402-410nm. Concentration of AgNPs in ablated solution was 50μg/ml and used as stock concentration in cell lines experiments. Ablated silver nanoparticles was capable of inhibit the growth of both HeLa and SKOV-3 cancer cell lines in dose and time dependent manner, whereas it was less inhibitory against normal fibroblast cells. All three apoptosis detection method conducted indicated positive characteristics for apoptosis, while GSH levels did not varied significantly in both HeLa and SKOV-3 cells. بطريقة فيزيائية من خلال الازالة بالليزر النبضي وبطاقة ) 800 ( ملي جول/نبضة. تم توصيف جسيمات الفضة )PVP( صنعة جسيمات فضة نانوية مغلفة بمادة المصنعة باستخدام مطياف الاشعة الفوق البنفسجية وقياس جهد الزيتا وتم قياس تركيز الجسيمات داخل المحلول المحضر بواسطة طيف الامتصاص الذري. كما تم توصيف شكل وحجم جسيمات الفضة المصنعة باستخدام المجهر الالكتروني. جرى مقارنة تاثير جسيمات الفضة النانوية على نوعين من الخطوط السرطانية )سرطان عنق الرحم وسرطان المبيض( مع خط الخلايا الطبيعي )خلايا جنين الفار(. اظهرت النتائج بأن متوسط حجم جسيمات الفضة النانوية المصنعة كانت 402-410 (, وان التركيز الكلي لجسيمات الفضة المصنعة كانت nm( وذات شكل كروي تقريبا, كما اظهرت رنين بلازمون سطحي يتراوح بين )nm 28,43( وبفترات حضن ) 24 و 48 )μg/ml عوملت الخلايا بعدة تراكيز متدرجة لجسيمات الفضة النانوية ) 0,78 و 1,56 و 3,125 و 6,25 و 12 و 5 و 25 .)μg/m 50( لها تاثير تثبيطي عالى على كل من خلايا سرطان الرحم والمبيض اعتمادا )PVP( و 72 ساعة(. بينت فحوصات السمية, ان جسيمات الفضة المصنعة المغلفة بمادة خلال 24 ساعة من التعريض )IC على التركيز والوقت, بينما اظهرت مستوى تثبيطي واطئ على خلايا الفأر الطبيعية. تم تحديد التركيز المثبط النصفي ) 50 على التوالي, بينما لم يكن هناك مستوى تثبيطي نصفي على خلايا الفأر الطبيعية. )μg/ml و 12,5 μg/ml في كل من خلايا سرطان الرحم والمبيض ) 3,75 على كل من خلايا سرطان الرحم والمبيض. وقد اظهرت النتائج بان جسيمات الفضة النانوية المصنعة قد حثت )IC اختبرت سمية التركيز المثبط النصفي ) 50 كثيرا مقارنة GSH بينما لم يتغير مستوى ,DNA على حدوث الموت المبرمج في المايتوكوندريا وفي انوية الخلايا وكذلك حدوث تقطع في مادة الخلايا الوراثية في علاج انواع مختلفة من السرطانات. )PVP( بخلايا السيطرة )الغير معاملة(. تشير هذه النتائج الى امكانية استخدام جسيمات الفضة المصنعة والمغلفة بمادة


Article
Epidemiology Study for Cancer Incidences among Patients from Baghdad Carrying Different Types of Cancer
دراسة ديمغرافية للحالات السرطانية المختلفة لمرضى من مدينة بغداد

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Abstract

This study was carried out in Al-Yarmook hospital, laboratories department, Baghdad- Iraq; One hundred thirty three Iraqi patients have been recorded during period May 2014 until December 2014. The current study has demonstrated that five different types of the following cancers: Breast cancer, Skin cancer, colorectal cancer, Bladder cancer and endometrial cancer were enrolled in this study. Comparison among each type of cancer was regarded in age, sex and number. The majority results in cancer epidemiology for the present study were fluctuated between the skin and breast cancer, which showed 33.1% and this, represented the prevalence of cancer among all the other types of cancers. In case of other types, the result has showed 16.5 %, 13.5% and 3.7 % for endometrial cancer, Bladder cancer and colorectal cancer, respectively.تمت الدراسة في مستشفى اليرموك – قسم المختبرات في بغداد. حيث جمعت مئة وثلاث وثلاثون حالات مرضية لمرضى مصابين بسرطانات مختلفة من . السجلات الخاصة بهذا المرض للفترة من مارس 2014 و حتى شهر كانون الاول من عام 2014 اظهرت الدراسة وجود خمس انواع مختلفة من السرطان مصابوب بها المرضى وهي سرطان الثدي والجلد والقولون والمثانة والرحم .تم استخدام المقارنة والاحصاء بين الانواع المختلفة وضمن النوع الواحد للسرطان على اساس العمر والجنس والاعداد . اظهرت النتائج تفوق سرطان الثدي و الجلد على بقية السرطانات الاخرى حيث كانت بنسب 33.1 % لكليهما اما بقية الانواع الاخرى فاظهرت النسب التالية % 16.5 لسرطان الرحم و % 13.5 لسرطان المثانة و 3.7 % لسرطان القولون.


Article
Accuracy of Perineal Percutaneous Fine Needle Aspiration Cytology (FNAC) of Prostate under Ultra- Sound in Comparism with Transrectal Aspiration in Diagnosis of Prostatic Cancer

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Abstract

Background: Prostatic carcinoma is the commonest internal malignancy of adult males. Patients usually presenting with voiding symptoms. Clinical suspicion of prostatic carcinoma depends on finding of one or more of the followings; hard prostatic nodule(s) on digital rectal examination (DRE), hypoechoic lesion on transrectal ultrasonography (TRUS), and/or high serum level of prostatic specific antigen (PSA). Final diagnosis depends on FNAC and/or histopathology. Objective: This is a case control prospective study designed to evaluate the usefulness of FNAC of prostate through perineal skin under ultrasound instead of transrectal aspiration in diagnosis of suspicious prostatic conditions. Patients and Methods: One hundred and ten males with voiding symptoms and clinical suspicion of prostatic carcinoma were participated in the study. Their ages were between 45 and 92 years. The aspiration was carried out as outpatient procedure. Results: Cytopathological results were; 64 (58.18%) positive, 4 (3.64%) suspicious, and 40 (36.36%) negative for prostatic cancer. Two specimens (1.82%) were inadequate for proper cytopathology. Results of histopathological examination of resected specimens from the same patients was very close to that of cytopathology with only two false positive and one false negative results. There was no any mentioned complication following aspiration, and sensitivity, specificity, and accuracy were 98%, 95%, and 91.81% respectively, and all results were statistically significant with p-values <0.05. Conclusion: Percutaneous perineal FNAC of prostate under ultrasound is a safe, reliable, cost effective, and as accurate as transrectal FNAC in diagnosis of prostatic cancer.


Article
Chromosomal study for Assessment of Recurrent Spontaneous Miscarriage by Fluorescence in situ Hybridization (FISH) technique in Erbil City Iraqi- Kurdistan Region
دراسة كروموسومية لتقدير الاجهاض التلقائي االمتكرر بواسطة تقنية التهجين في الموضع المفلور في مدينة اربيل / اقليم كردستان العراق

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Abstract

Spontaneous miscarriage is the most frequent complication of pregnancy and, classically, defined as the occurrence of at least two miscarriages before 20 weeks of gestation. Over 50 % of first trimester pregnancy losses are attributed to chromosomal abnormalities. This study aimed to investigate aneuploidy in spontaneous miscarriage by fluorescence in situ hybridization (FISH) using probes for 13, 18, 21, X and Y chromosomes. A total of 100 tissue samples from concepts materials were collected and examined by FISH. The incidence and type of chromosomal abnormality and sex ratio were analyzed for each samples. Moreover, the relationship between the rate of aneuploidy and maternal age also the relationship between maternal age and type of aneuploidy and the difference in incidence of aneuploidy between samples from previous miscarriage and those with no previous miscarriage were investigated. Results obtained from this study revealed that, 52 of 100 cases were with aneuploidy. Trisomy 21, 18, and 13 was the major aneuploidy followed by monosomy X. Cases of miscarriages which contain chromosomal abnormalities were higher in females than males. Cases with advanced maternal age and history of previous miscarriage were significantly have higher aneuploidy rate compared with young age cases and those with no previous miscarriage. However, rates of trisomies 18, 13, and 21 of the advanced maternal age group were remarkably higher than those of the young maternal age group.ان الاجهاض التلقائي هي واحدة من التعقيدات الاكثر تكرارا اثناء الحمل وتعرف على انه حدوث على الاقل اجهاضين قبل الاسبوع العشرين من الحمل.فوق 50% من حالات الثلث الاول من الحمل تفقد والتي ترجع الى التشوهات الكروموسومية.ان هذه الدراسة تهدف الى البحث عن التضاعف الناقص في الاجهاض . Y و X، التلقائي بواسطة تقنية التهجين في الموضع المفلوربواسطة استخدام مسبرات لكروموسومات 13،18،21 تمت جمع وفحص 100 نموذج نسيجي من بقايا الحمل بواسطة تقنية التهجين في الموضع المفلور،قد تمت تحلبل حدوث ونوع التشوهات الكروموسومية لكل نموذج.تمت دراسة العلاقة بين معدل التضاعف الناقص و عمر الام وايضا العلاقة بين عمر الام ونوع التضاعف الناقص والفرق بين حدوث التضاعف الناقص بين النماذج المؤخوذة من الحلات التي لها اجهاضات سابقة والاخرى التي ليست لهااجهاضات سابقة.تمت الحصول على النتائج من هذه الدراسة انه 52 من 100 حالة الاجهاض كانت لها التضاعف الناقص. ان التثلث الكروموسومات 13،18،21 كانت اكثر انواع التضاعف الناقص يليهاتشوه احادى الكروموسومي. لقد لوحظت ان حالات الاجهاضات التي تحتوي على التشوهات الكروموسومية عالية في الاناث مقارنة بالذكور. ان حالات التي فيها تقدم في عمر الام وتاريخ عائلي لاجهاضات سابقة كانت معنويا لهل معدل تضاعف ناقص عالي مقارنة بحالات صغر عمر الام مع من هم ليست لديهم تاريخ باجهاضات سابقة. ان معدلات تثلث الكروموسومات 13،18،21 في المجموعة التي فيها تقدم عمر الام كانت ملحوظة بصورة عالية مقارنة بالمجموعة التي فيها اعمار الام صغيرة


Article
Mitotic activity of cultured human lymphocytes treated with gold nanoparticles

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Abstract

Potential health and environmental effects of nanoparticles need to be thoroughly assessed before their widespread commercialization. The present investigation was planned with the aims to determine the effects of gold nanoparticles (GNPs) on blast (BI) and mitotic (MI) indices of cultured lymphocytes. The results revealed that BI (50.3 ± 2.3, 30.2 ± 1.9, 10.5 ± 0.7 and 0.0%, respectively) and MI (70.1 ± 2.9, 20.4 ± 1.1, 5.3 ± 0.1 and 0.0%, respectively) showed a gradual decreased percentage as the concentration of GNPs was increased from 0.085 to 0.66 μg/mL, and the difference was significant compared to control culture (81.6 ± 2.5 and 90.2 ± 3.7%, respectively). A maximum inhibition of BI and MI was occurred at the concentration 0.66 μg/mL. In conclusion, GNPs can be considered as a growth inhibiting agent, but it is too early to reach a final conclusion in this regard and further investigations are required to elucidate the biochemical mechanisms involved in inhibiting the mitotic activity of lymphocytes.


Article
Pathological study on some common bone tumors in human

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The study aimed to find the most common bone tumors , affected ages and to identify gross and microscopical appearances . Thirty bone tumor were obtained ; 28 cases diagnosed as primary bone tumor and 2 cases diagnosed as secondary bone tumors . The primary benign bone tumors (13) while malignant bone tumor (11) and the rest (4) cases were tumor like conditions. The primary bone tumors are diagnosed as follows osteoid osteosarcoma (4),osteoma(2),chondroblastoma(1),osteochondro ma(6)chondrasarcoma (2), fibrosrcoma (1) , giant cell tumors (5) , hemangiosarcoma (1) , ewing sarcoma (1) , lymphoma (1) . aneurysmal bone cysts (3) and fibrous dysplasia (1) . The secondary bone tumors (2) metastatic bronchial carcinoma case and another case poorly differentiated prostatic carcimoma Conclusion: Osteosarcoma is the most common malignant primary bone tumors and osteochondroma is the most common benign primay bone tumor .


Article
Potential Cytotoxic Effect of Crude Extract Produced by Lactobacillus spp on Caco-2 & L20B Cell Lines in vitro

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This study was designed to determine the cytotoxic effect of crude products which was produced by isolates of Lactobacillus spp against three cell lines using 3-(dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide (MTT assay). About 72 samples were collected from local dairy products and infant feces with age of (1- 40 days) in Baghdad, the collection of those samples last long (September 2016 to January 2017). All isolates were identified as Lactobacillus spp according to their morphological, cultural, biochemical features and PCR technique by 16SrRNA gene. The cytotoxicity of crude extract was evaluated on cancer intestinal cells of mice L20B, human colon adenocarcinoma Caco-2 and human hepatic WRL-68 cell lines. The results showed that crude extract had cytotoxic effect on L20B with IC50= 17 g/ml and on Caco-2 with IC50=221.9 g/ ml in concentration 400 g/ml for both. While the crude extract of WRL-68 cells did not show when treated with the same concentration when used WRL-68 as normal cells for comparison. Conclusion: In last years, the high prevalence rate of many types of cancer in Baghdad, especially of intestinal cancer in human, so had used materials was produced by Probiotic bacteria to study their effects on the cancer cells in vitro.


Article
Olea europaea leaves extract downregulate Newcastle disease virus gene expression in cancer cells

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Abstract

Many researches have been made to find antiviral agents from natural sources. Leaf of Olea europaea (OLE) is one of the most important medicinal plants, as it is a well-known source of different phytochemicals. This study was carried out to investigate the effect of using Olea europaea leaves extract on the level of viral gene expression (M and F) in Hela cell line by using the qRT-PCR. Also, we examined the adding of interferon-beta ( IFN-b ) as an antivirus factor and analyzed the expression of viral and IFN-b genes to identify viral targets for OLE extract. First, we propagated the virus in embryonated chicken eggs and showed to kill embryos in different times intervals (less than 72 hours) with marked sever hemorrhage in infected embryos, and agglutination activity reflects titer (1024) and 1×10 6.4 (TCID50). Hela cells were cultured and different concentration of OLE extract was added; cell viability was measured by MTT assay. Gene expression showed that the expression of the viral gene was downregulated in the present of OLE extract. Pretreatment with IFN-b downregulated viral gene expression higher than the extract, also evaluated the IFN-b concentration by ELISA assay. OLE can be considered as antivirus agent and used this property to help in control Newcastle disease.

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Article
Growth inhibition of mice mammary carcinoma cell line with green synthesized Magnetic Iron Oxide Nanoparticles

Authors: Amer T. Tawfeeq --- Ghassan M. Sulaiman --- Amal Naji
Pages: 234-238
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Mammary adenocarcinoma represents one of the most cancers that effect woman in Iraq and worldwide. Finding a definite cure for this type of cancer is a target of considerable research in many laboratories over the globe. In this study, a green synthesized magnetic iron oxide nanoparticles (MNP) was used to specify their ability to inhibit the growth of mice mammary carcinoma cell line (AMN3), it comes as a preliminary study of wider ongoing research. After successfully synthesizing magnetic iron nanoparticles, it employed in different concentrations to expose AMN3 cells for 24 and 48 hours. The viability of the exposed cells was determined using standard MTT assay. The inhibition concentration 50 was determined afterward and used to test the possibility of inducing apoptosis in the carcinoma cells using a mixture of fluorescent stains acridine orange and propidium iodide. Results indicated that green synthesized MNP can inhibit the growth of mammary adenocarcinoma cell line up to 79% and 89% after 24 and 48 hr of incubation with 1000μg/ml. The IC50 of MNP was capable of inducing apoptosis in this cell line as shown by fluorescence microscope. The standard hemolysis assay used to test the reaction between the green synthesized magnetic oxide nanoparticles and normal cells. The IC50 of AMN3 cells growth inhibition only induce 14% of RBC hemolysis, while the percentage induced by negative control was 10%. In conclusion, green MNP have an effect upon cancer cells greater than normal cells. This material must tested against other types of cancer and normal cells to verify its action and study mechanisms of apoptosis induction in detail.


Article
تثبيط الخلايا السرطانية باستخدام الخلايا المقتولة حرارياً لبكتريا Lactobacillus acidophilus المعزولة من براز الأطفال و مصادر غذائية مختلفة

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منذ تعرف الانسان على مرض السرطان ولحد الان , فهو مازال التحدي الاكبر له . أذ بالرغم من وجود العديد من العلاجات المختلفة سواء الكيميائية والفيزيائية والجراحية , فأنها لم تسطيع أن تحقق النتائج المرجوة كما أنها لها أضرار جانبية , لذلك سعى الباحثون الى إيجاد طرائق علاجية جديدة وبديلة . ومن هذه البدائل قيام المختصين في مجال الاحياء المجهرية بمعالجة الاورام السرطانية وذلك عن طريق استخدام بكتريا حامض اللاكتيك ونواتجها الايضية . لذلك أجريت هذه الدراسة acidophilus Lac


Article
دراسة وراثة خلوية عن اورام القولون والمستقيم في الانسان

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تضمن البحث الحالي اجراء دراسة مرضية وراثية خلوية على ) 29 ( عينة ورمية لمصابين باورام القولون والمستقيم وكذلك دراسة وراثية خلوية للخلايا اللمفاوية في الدم المحيطي لنفس المصابين. أجريت الدراسة الوراثية الخلوية على نفس العينات الورمية حيث استعملت طريقة الزرع المباشرة وكذلك على الدم المحيطي لنفس المرضى باستعمال طريقة الزرع لفترة قصيرة. تم النجاح في تحليل الكروموسومات ل ) 7( حالات باستخدام تقنية التحزيم بالنسبة للعينات الورمية و) 22 ( حالة بالنسبة لعينات الدم المحيطي. أظهرت حالتين اما الحالة الثانية فقد أظهرت زيادة للكروموسوم )xy,+7,47( ) للخلايا الورمية تغيراً مفرداً واحداً. الحالة الأولى أظهرت زيادة في نسخ كروموسوم ) 7 .Y,#20,#18,#17,#11,#7,#5,# متناظر الذراع الطويل. اما بقية الحالات فقد أظهرت تغيرات تركيبية وعددية مختلفة شملت كل من كروموسوم # 1 )X (i1q( )1( اما الدراسة الوراثية الخلوية للخلايا اللمفاوية في الدم المحيطي فقد أظهرت وجود خلايا رباعية المجموعة الكروموسومية في حالتين فقط. الاستنتاجات: لوحظ تكرر التغيرات للكروموسوم 1# ، وكذلك تؤكد النتائج الحالية علاقة زيادة كروموسوم # 11 بمراحل تطور سرطان القولون والمستقيم.

Table of content: volume:10 issue:2