Table of content

Basrah Journal of Veterinary Research.

مجلة البصرة للابحاث البيطرية

ISSN: Print:18138497 E; 24108456
Publisher: Basrah University
Faculty: Veterinary Medicine
Language: English

This journal is Open Access

About

. Basrah Journal of Veterinary Research (Bas.J.Vet.Res.) is the official journal of College of Veterinary Medicine, University of Basrah, Iraq. The Journal is a biannual international peer-reviewed, open access research publication covering all aspects of veterinary science. The journal publishes original papers in English, case reports and reviews relevant to any area of veterinary sciences and veterinary medicine.

Loading...
Contact info

Mobile;07801034925
E.mail;alaasawad24@gmail.com

Table of content: 2015 volume:14 issue:1

Article
PREVALENCE OF GASTROINTESTINAL NEMATODES PARASITES FROM SHEEP AND EVALUATION OF SOME ANTHELMINTHIC RESISTANCE IN ERBIL GOVERNORATE

Loading...
Loading...
Abstract

Prevalence and anthelmintic resistance status of gastrointestinal nematodes in 430 sheep investigate by faecal examination was conducted in Qushtapa, Khabat, Benslawa, Salahaddin subdistricts in Erbil, from April to July 2012. The mean eggs per gram (EPG) count determined by using modified McMaster technique, showed that (40.46%) of the sheep were infested, the higher infection was 17.2% Nematodirus spp., 13.02% Strongylus spp., 4.18% Marshaligia spp., 2.79% Trichurus spp. and 3.25% with coccidia. Four farms were selected randomly each divided to control and three treatment groups: Levamisol, Albendazole and Ivermectin groups. Anthelmintic efficacy was measured after treatment dates were investigated with faecal egg count reduction test, the FECRT percentage for Levamisol in sheep ranged from 80.3 to 94.7 which show most effective in Khabat with FECRT of 94.7% while with Albendazole ranged from 75.5% to 80.2%, suspected resistant in all group, but the injectable Ivermectin in sheep ranged from 85.2 to 94.8 and show effective in Bnaslawa and Salahaddin with FECR of 94.6% and 94.8% respectively. All the anthelmintic were found to be low effective, but resistance to Albendazole was suspected. Based on the findings, it was concluded that development of anthelmintic resistance could be prevented by avoiding frequent dosing and under dosing, while strategic deworming should be practiced by both animal health workers and animal owners.


Article
-HEPATOPROTECTIVE AND HYPOLIPIDEMIC EFFECTS OF BIS [4-(4'-HYDROXY-3'-METHOXYBEZYLIDINEAMINOPHENYL) ]TELLURIDE (R2TE) AGAINST SODIUM NITRITE INTOXICATION IN MALE ALBINO RATS.

Loading...
Loading...
Abstract

Sodium nitrite is widely used as a color fixative and preservative in meat and fish. Impairment of hepatic function and disturbances in lipid metabolism are well recognized adverse effects of sodium nitrite. The aim of this study is to investigate the role of bis [4-(4'-hydroxy-3'-methoxybezylidineaminophenyl)]telluride, a novel compound, in preventing the hepatic damage and disturbances of lipid metabolism induced by sodium nitrite toxicity in male albino rats. The estimated LD50 of [4-(4'-hydroxy-3'-methoxybezylidineaminophenyl)]telluride in adult male albino rats is 218.7 mg/kg body weight. Rats given sodium nitrite (0.2%) in the drinking water showed a significant increase in serum ALT, AST, ALP, Total cholesterol, TG. LDL and VLDL while HDL significantly reduced. These changes are reversed by administration of bis [4-(4'-hydroxy-3'-methoxybezylidineaminophenyl)]telluride in a dose of 11mg/kg body weight corresponding to 1/20 LD50. It is concluded that bis [4-(4'-hydroxy-3'-methoxybezylidineaminophenyl)]telluride is effective in preventing hepatic damage and dislipidemia in sodium nitrite intoxicated male rats.


Article
-DETECTION OF FOOT AND MOUTH DISEASEـVIRUS INFECTION ASSOCIATED ANTIBODIES IN SHEEP SERA IN BASRA BY AGID TEST

Loading...
Loading...
Abstract

Foot and mouth Disease (FMD) is the most contagious disease of the clovenhoofed animals and has a great potential for causing severe economic losses in susceptible cloven-hoofed animals, of the domestic species, cattle, pigs, sheep, goats and buffalo are susceptible to FMD, in addition many species of cloven- hoofed wildlife such as deer, antelope and wild pigs may became infected (1). Infection of susceptible animals with FMD virus lead to the appearance of vesicles on the feet, in and around the oral cavity and on the mammary gland in females, the severity of the clinical signs varies with the strain of virus, the exposure dose, the age and breed of animal, the host species and its degree of immunity (2). There are seven serotypes of FMD virus namely O, A, C, SAT1, SAT2, SAT3, and Asia1, infection with any serotype dose not confer immunity against other (3). In Iraq, the serotypes A, O, and Asia1 were recorded in years 1952, 1957, and 1975 respectively (4). In the period between the end of 1998 to the beginning of 1999 an sever outbreak of FMD occurred in Iraq, it affected cows, buffalos, sheep and goats and may be other animals, the virus isolated from cow, buffalo, and sheep, the disease is still endemic in Iraq (5). FMD is still prevalent in many parts of the world as emphasized by the 2001 epidemics in the European Union, southern Africa, Asia and South America (6). (7) reported the finding of third antigenic component associated with infection with Foot and Mouth Disease Virus, called virus infection associated antigen (VIA), which reacted with the sera from convalescent animals but not with sera from vaccinated animals, VIA antigen is specific for FMD but is not virus type-specific, as antiserum to six of the seven virus types gave positive agar gel diffusion precipitin (AGID) reaction with VIA antigen prepared from type A infected tissue culture fluids. Antibody against VIA was originally demonstrated only in sera from animals infect with FMDV and not in sera from animals immunized with inactivated vaccines (8). VIA antibodies in sera from animals convalescent with FMD have been detected by Immunodiffusion in agar gel (AGID) test (9). The antibodies against the VIA antigen were detected between the second week and 13 th or 14 th week after infection (10). The detection of antibodies to virus infection associated antigen was done in Iraq by (11), the survey was done on Iraqi cow sera, collected from Mosul slaughter house in Mosul Government. By the Agar Gel Immuno-Diffusion, samples were examined.


Article
-BIOCHEMICAL EFFECTS OF TRIGONELLA FOENUMGRAECUM L.SEEDS IN NORMAL AND ALLOXAN INDUCED DIABETIC RABBITS

Loading...
Loading...
Abstract

This study was undertaken to investigate the effect of ethanolic extract of Trigonella foenum-graecum L. (fenugreek) seeds on blood glucose, total serum cholesterol and total serum protein in normal and alloxan induced diabetic rabbits. Twenty-four mature male rabbits (local breed) were randomly divided into four groups of equal number: (Control negative group (normal rabbits received 2 ml normal saline). Normal animals group treated orally with (200 mg /kg b.w.) alcoholic extract of fenugreek daily. Diabetic positive control group (induced diabetic rabbits received 2 ml normal saline). Diabetic group treated orally with (200 mg /kg b.w.) alcoholic extract of fenugreek daily. All group were deranched for six weeks. Diabetes was induced experimentally by single intravenous injection of alloxan monohydrate (100 mg/ kg b.w.).Estimation of blood glucose, total cholesterol and total protein was carried out weekly for all groups of the experiment. The results showed that alcoholic extract of T. foenum-graecum L. caused Significant decrease (P<0.05) in the levels of serum glucose and total cholesterol in diabetic treated groups after one week of administration. Non significant decrease in blood glucose concentration of normal treated rabbits compared with normal control group. Moreover significant increases (P<0.05) in total serum protein in diabetic treated groups. This study concluded that the alcoholic extract of T. foenum-graecum can lower blood glucose, total serum cholesterol and improve total serum protein.

Keywords


Article
- EFFECT OF SEASON ON SOME HEMATOLOGICAL, BIOCHEMICAL AND SOME HORMONE OF LOCAL IRAQI BLACK FEMALE GOATS

Loading...
Loading...
Abstract

This study was conducted at animal farm / Collage of Agriculture, Basrah University, during the period from April to December . A total of 8 blood samples were taken from dry female goats at (2.5-3.5) years old during first day of April , July , October , December months .The aim of this study to evaluate the effect months of year on blood (WBC , RBC, PCV and Hb) and biochemical parameters (Fe , Phosphate, Albumin, Total protein, Cholesterol and Glucose in addition to some hormones like Estrogen and thyroxin. The present study resulted that there were significant (P<0.05) increase in blood red cell count , total white blood cell number , and monocytes percentage in summer compared with spring and winter . Hemoglobin concentration and PCV percentage were significantly (P<0.05) increased in summer compared with other seasons. Iron concentration was highly significantly (P<0.05) increased in summer and autumn in comparison with other seasons. The results also showed that there were significant (P<0.05) increase in Estrogen and decrease in thyroxin hormone concentration in summer compared with other seasons.

Keywords

Goat --- haemoglobin --- WBC.


Article
-SURVEY OF PATHOGENIC BACTERIA IN FRESHWATER CRAB (POTAMON MESOPOTAMICUM) COLLECTED FROM TIGRIS , BASRAH.

Authors: Ghazi maleh Al-Malki
Pages: 62-69
Loading...
Loading...
Abstract

The present study was carried out to determine the presence of pathogenic bacteria in freshwater crab (Potamon mesopotamicum) in Tigris at North of Qurna, as well as their antibiotic resistance. A total of 21 bacterial isolates consisting three bacterial species were isolated from freshwater crab, P. aeroginosa, E. coli, A. hydrophila kanamycin was found to be effective against all the bacterial isolated whilst the highest percentage of antibiotic resistance was shown beyond to Lincomycin (76.4%) followed by ampicilin(66.14%), Erythromycin(57.3%).

Keywords

freshwater --- Lincomycin --- E. coli.


Article
HISTOLOGICAL AND HISTOCHEMICAL STUDY OF THE LIVER OF IRAQI LOCAL DUCKS

Authors: Maha Abd Al- Hadi Al- Abdulla
Pages: 70-78
Loading...
Loading...
Abstract

The aim of study is provide basic data about the histological features of the liver of local Iraqi ducks and to study the glycogen content in the cytoplasm of the hepatocyte. The anatomical examination showed that liver laid cranial part of the abdominal cavity caudally and ventrally to the heart and associated to the gizzard. The liver of Mallard is divided into two undivided lobes right and left lobes (the right lobe is larger than left lobe). Histological examination revealed that the liver consist of several lobules separated from each other by thin trabeculae of connective tissue extend from delicate capsule that enclosed the liver. The basic unit of the parenchyma of liver is hepatocyte which arranged in plates or cords like that radiated around the central vein and between these cords there is sinusoids lined by alayer of fenestrated endothelial cells and Kupffer's cells. In the boundary of each lobule showed portal area which consist of hepatic artery; hepatic vein and bile duct lining by cuboidal cells. The histochemical study showed formed differ size of glycogen granules in cytoplasm of hepatocytes and in some speciment the glycogen large granules arranged around the central vein because the bird in starvation period .the liver of Mallard generally is closely similar to the liver of another avian birds and vertebrates

Keywords

Hepatocyte --- Glycogen --- Ducks.


Article
EFFECT OF PASSIVE IMMUNIZATION AGAINST INHIBIN-Α, -ΒA, AND –ΒB SUBUNITS ON SERUM GROWTH AND DIFFERENTIATION HORMONES PROFILE IN PREGNANT AND LACTATING PRIMIPAROUS WISTER RATS

Loading...
Loading...
Abstract

Transforming growth factor β (TGFβ) superfamily members are closely associated with tissue remodelling events and reproductive processes. The present study aimed to determine the role of immunoneutralization of endogenous inhibin-α, βA, and βB subunits on serum growth growth and differentiation hormones profile during pregnancy, delivery, and lactation periods in primiparous female Wister rats.Eighty four pregnant primiparous female rats were assigned to 4 groups (21 per each). On 5 th and 10 th day of gestation, control was injected with saline (100μl, i.p.), Ta, Tba, and Tbb groups were injected with inhibin-α, βA, and βBantiserum (1µg in 100μl of saline, i.p.), respectively. Each group was allocated to 3 equal subgroups: pregnancy, delivery, and lactation subgroups were sacrificed on day 16 of gestation, 1st day after parturition, and 11 th day of lactation, respectively. At the end of each subgroups period, females were anesthetized, dissected and blood samples were obtained for assessment of inhibin-A, -B, activin-A , -B, -AB, GH, and prolactin levels. Serum inhibin-A concentration in Tbb group increased during pregnancy and delivery among experimental groups. In comparison between periods, Tbb group showed significantly higher level during pregnancy and decreased during delivery and lactation, whereas Ta and Tba groups recorded no difference between periods. Inhibin-B increased in control and Tba groups during pregnancy, whereas lactation period showed higher levels in Ta, Tba, and Tbb groups compared with control. Ta, Tba, and Tbb groups recorded no siginificant differences between pregnancy and delivery periods but they were significantly higher in lactation period.During pregnancy and delivery, Tbb group revealed higher levels of activin-A and lowest level of activin-B concentrations among groups, whereas activin-AB concentration increased in control and Ta groups. In comparison between periods, activin-A concentration was higher at delivery, whereas activin-B and –AB concentrations were higher at lactation. Serum GH, in Ta group, recorded higher level during the three periods among groups. In comparison between periods, the levels of GH and PRL in all groups showed higher levels at delivery followed by lactation and pregnancy.In conclusion, passive im munization against inhibin-α, βA, but not βB subunit, at 5th and 10th day of pregnancy, have ameliorating role on serum growth and differentiation hoemones profile


Article
MORPHOLOGICAL AND MORPHOMETRICAL CHANGES THAT OCCUR IN ENDOMETRIUM OF AWASI EWES DURING DIFFERENT STAGES OF PREGNANCY

Authors: A. M. Elias
Pages: 97-104
Loading...
Loading...
Abstract

Sixteen utri of Awasi sheep at different stages of pregnancy were used in this study .The intercaruncular surface epithelium was pseudostratified at the early stage of pregnancy and then changed gradually to simple columnar after day 50 0f pregnancy. At day 118 it become low columnar or simple cuboidal and decreased in height . The glandular epithelium was mainly simple columnar and showed high secretory activity during the studied of pregnancy stages. The glandular diameter and their lumen was increased with the advancement of pregnancy . The gland showed saculation at late stages of pregnancy .

Keywords

Awasi sheep --- epithelium --- pregnancy.


Article
TOXICITY AND ANTIMICROBIAL ACTIVITY OF 6-CHLORO-2,4-DIAMINO- PYRIMIDINE

Loading...
Loading...
Abstract

In recent years, pyrazole and pyrimidine derivatives attracted organic chemists due to their widespread potential biological and chemotherapeutic activities. In this study, pyrimidine derivative namely 6-Chloro-2,4-diamino pyrimidine was screened for antibacterial activity against Staphylococcus aureus, Escherichia coli, Bacillus cerius, Streptococcus spp, Klebsella- spp and salmonella spp and fungicidal activity against Aspergillus multi, Aspergillus niger and Candida albicans. A compound exhibited low antibacterial and antifungal activity with the reference standard Streptomycin, Vancomycin and Nystatin respectively. The toxicity of the compound was also assayed via the determination of their LD50 value by using Dixon , s up and down method (1980). Studied compound was found to have an LD50 of 518.6 mg / kg of body weight.


Article
HISTOLOGICAL LESIONS OF SLAUGHTERED CALVE'S KIDNEYS IN MOSUL SLAUGHTER HOUSE

Authors: H. K. Ismail
Pages: 112-123
Loading...
Loading...
Abstract

The objective of present study was to investigate the gross and histopathological findings of calves kidneys slaughtered in Mosul abattoir. Pathological examination were carried out on fifty calves' kidneys. The most common gross lesions were included 20% multifocal white spots, 16% enlarged pale kidneys20% congested enlarged kidneys , 10% small shrunken kidneys, 14% hyperemic hemorrhagic kidneys, 20% kidneys without any gross lesions. The common observation in the histopathological examination of the kidneys revealed slight to severe mononuclear infiltration were commonly observed, widening of the Bowman's space were common, nephrosis, glomerulonephritis, interstitial nephritis, acute tubular degeneration and necrosis. Cysts were observed also in this study.


Article
TOXOPATHOLOGICAL STUDY OF METHAMYL EFFECT ON THE ROCK PIGEONS (CULUMBA LIVIA GADDI)

Loading...
Loading...
Abstract

This Study was conducted in animal house in Faculty of veterinary medicine of university of kufa. This study was designated to assess the neuropathy of Organophosphorus pesticide (Methomyl) after long–term administration of low dose in Rock pigeons. The Clinical signs, gross lesions and histopathological assessment of nervous tissue were described. A total of 20 pigeons, 3months old and about 500g average body weight pigeons were used in this study. The pigeons were equally divided in to tow groups. The dose was calculated based on study at close pilot at 0.02 ml and gradually lose the dose down to the toxic non-lethal dose. The toxic dose was 0.01 ml. One group was daily administered via oral gavages with 0.01 ml of methomyl; for 21 days where as the other group were leave control without administration. Organophosphorus pesticide treated groups exhibited both muscarinic and nicotinic signs of toxicity.

Keywords

Methomyl --- toxicity --- pigeons.


Article
AMELIORATIVE ROLE OF SILYMARIN EXTRACTED FROM SILYBUM MARIANUM SEEDS ON NICKEL CHLORIDE INDUCE CHANGES IN TESTICULAR FUNCTIONS IN ADULT MALE RABBITS.

Loading...
Loading...
Abstract

This study aimed to investigate the Ameliorative effect of ethanolic extracted of silymarin from Silybum marianum seeds and to compare it’s with the commercial silymarin extract(legalon) against oral dosing of Nickel chloride effects on sperms concentration, motility ,viability, sperm abnormality and male fertility and gonadal hormones .Twenty adult male rabbits aged 5 to 6 month and weighted 1.250-1.500 kg divided into four equal groups,1 st group served as control group received 1 ml of normal saline (NaCl 0.9%),2 nd group received (1mg/100g B.W )NiCl2 orally, 3 rd group received same dose of NiCl2 plus (0.1mg/100g B.W) silymarin extract, the fourth group received same dose of NiCl2 plus (0.1mg/100g B.W) legalon for 35 days, Results showed negative effects of NiCl2 which caused significant(p≤0.05) decrease in sperm concentration ,viability, motility and fertility while sperm abnormality was significantly increase, also NiCl2 caused significant (p≤0.05) decrease in serum progesterone, estradiol and testosterone. while silymarin extract and legalon adverse the negative effects of NiCl2 and causing ameliorative effects on all the studied parameters .


Article
GROSSLY AND MICROSCOPIC STUDY OF THE TRACHEA AND BRONCHIAL TREE IN THE LOCAL SHEEP ( OVIS ARIS).

Authors: Saffia Kareem Wally Al- Umeri
Pages: 145-155
Loading...
Loading...
Abstract

The trachea of the local sheep is a tubular structure that begins at the cricoid cartilage of the larynx and ends when it bifurcates to form the principle bronchi, composed of series of incomplete rings that were incomplete dorsally present between of them longitudinal folds. The study include twelve specimens divided into four groups grossly, histological study, cast and radiography. The anatomical study include the mean total tracheal length from the first ring to the bifurcation about (25.9 cm) , mean number of cartilaginous rings (48.6), mean number of folds (45), measured the length of trachea from the cricoids cartilage to the trachealis bronchus which enter to the cranial lobe of lung which branches from the trachea in last third before divided into two bronchi right and left each of one enter the lung for respiration also measure diameters of each tracheal ring which deference according the position of rings, which decrease of diameters when direction caudally to the root of lung the rings have different shape in the three parts of the trachea cervical, middle and thoracic the histological study showed the trachea lining pseudo stratified ciliated columnar epithelium with goblet cells, the wall of trachea consist of mucosa, submuosa, hyaline cartilage and adventitia and have large amount of tracheal gland,the trachealis muscle composed of elastic fibers interupted with smooth fibers. The cast of trachea showed the bronchial tree and radiography explain the trachea , bronchi and bronchioles.

Keywords

Trachea --- Radiography --- Rings.


Article
LAPAROSCOPIC LIVER RESECTION IN THE GOAT

Loading...
Loading...
Abstract

The aim of this study is to evaluate application of laparoscopy in veterinary surgery and assess the feasibility safety of laparoscopic hepatectomy using thermal energy to control the bleeding after the partial hepatoctomy in the goats. liver enzymes and blood counts were drawn pre and postoperatively with recorded Rectal temperature, respiratory rate and pulse rate and record the time of returning to eat and drink post operation. The abdominal cavity was inflated by the introduction of co2 gas . The result showed that the laparoscopic partial hepatectomy can be easily done in goats without any complications. The mean time of the procedures was 45 minutes (range 40 to 60 min) . The physical parameters were measured preoperatively 1 st , 2 rd , and 3 th days postoperatively ,showed no significant increase in respiratory rate , plus rat and temperature .The Hematological parameters Hb, PCV and Biochemical tests AST,ALT,LDH, Showed no significant increase in number at day1,2,3 after the surgery From the results of this study, it was concluded that the laparoscopic partial hepatectomy can be easily done in goats without any complications.


Article
HISTOPATHOLOGICAL AND BIOCHEMICAL STUDYTO EFFECT OF CODEINE-PARACETAMOLIN SPRAGUE DAWLEY RATS

Loading...
Loading...
Abstract

This study performed in twenty four male Sprague Dawley rats for (90) days which divided randomly into four equal groups. Group (1): received normal saline daily. Group (2): received orally codeine-paracetamol(40/2500 mg / kg b.w) daily. Group (3): received orally codeine-paracetamol (80/5000 mg / kg b.w)daily. Group (4): received orally codeine- paracetamol (160 /10000 mg / kg b.w) daily. After end of day (90) of study all animals were sacrificed to do the histopathological and biochemical examinations. The statistical analysis results revealed the body weight effects of codeine-paracetamol toxicity a significant (P≤0.05) decreases of treated group after (90) days of treatment. The histopathological investigation of liver, kidney and brain of treated groups showed centrolobular necrosis, dilation of sinusoids, vaculation of hepatocytes and septal fibrosis of liver while kidney showed vaculation of mesengial cells of glomeruli, necrosis of proximal convoluted tubules and dilation of renal cortical tubules, also brain of treated group showed vaculation of neurons, these changes are appeared mild in group (2), moderated in group (3) and sever in group (4). The statistical analysis results of biochemical investigations of liver and kidney function tests showed a significant (P≤0.05) increases of levels of serum AST, ALT, ALP, bilirubin and creatinine respectively in all treated groups which these enzymes increased mildly in group (2), moderately in group (3) and severely in group (4).

Keywords

Paracetamol --- Toxicity --- Necrosis


Article
AN INVESTIGATION ON LIGHT COLOR AND STOCKING DENSITY ON SOME PRODUCTIVE PERFORMANCE OF BROILERS

Loading...
Loading...
Abstract

This study was designated to investigate the effect of light color and stocking density on some productive performance of broilers. A total of 675 Ross 308 one-dayold broiler chicks were used in this study. The birds were exposed to white light (WL) as a control , red light (RL), blue light (BL), green light (GL), and Blue – Green mix light (BGL) by a light-emitting diode system (LED) applied for 24 hours daily in separated rooms. The birds were randomly divided and housed into 9 wooden sealed pens of 1m 2 in three replicates for each density 12, 15 and 18 birds/m 2 in the room. The results showed that the broilers reared under BGL significantly increased (P< 0.05) body weight (BW) and weight gain (WG) at 1 st , 3 rd and 5 th week. Obviously, stocking density had no significant effect on BW and WG at 1 st week but significantly increased under 12 birds/m 2 at 3 rd and 5 th week. Feed intake (FI) recorded a significant increase (P< 0.05) in broilers reared under WL at 1 st week, but no statistical difference was found at 3 rd week whereas, FI significantly increased (P< 0.05) in broilers reared under BL at 5 th week. The stocking density affected FI values which were significantly differed (P< 0.05) at 1 st , 3 rd and 5 th week under 12 birds/m 2 compared with other densities. The results of the study revealed a significant increase (P< 0.05) of Feed conversion ratio (FCR) in broilers reared under BL at 1 st week and RL at 3 rd week but there was no significant difference between groups at 5 th week. The results indicated a significant difference of FCR value in 18 birds/m 2 at 1 st week but there were no significant differences at 3 rd and 5 th week at different densities. In conclusion, the results of this study indicated that chickens reared under five different color lighting schedules with three bird densities showed that broilers reared under mixing blue – green light under 12 birds/m 2 has a significant positive effects on production performance compared with other light programs and bird densities

Keywords

broilers --- Red light --- Body weight.


Article
EFFECT OF BISPHENOL A ON THYROID, LIVER AND TESTICULAR FUNCTIONS IN ADULT MALE RATS

Authors: Jassim. M. A. Alkalby
Pages: 187-206
Loading...
Loading...
Abstract

This study was carried out in Veterinary Medicine College / University of Basrah to investigate the effect of Bisphenol A on thyroid, liver and testicular functions. A total of 24 adult male rats were randomly divided into four equal groups, six animals in each group. Animal of group (1) served as control and received a daily oral administration of corn oil throughout the experimental protocol. Animals of group 2, 3 and 4 were administered orally 50,100 and 200 mg/kg body weight of BPA respectively dissolved in corn oil, the experiment extended for 30 days. The results of the present study showed a significant decrease in serum thyroxin (T4) concentration and a significant increase in serum aspartate aminotransferase ( AST) and alanine aminotransferase ( ALT) concentration in bisphenol A treated groups. A significant decrease in serum testosterone and LH concentrations in all BPA-treated groups compared with control. On other hand non significant decrease in serum concentrations of FSH were observed in BPA-treated groups compared with control. A significant decrease in epididymal sperm count and sperms motility in all BPA treated groups. However no significant differences were noted in sperms viability between all BPA treatment groups and control. Histopathological changes were found in thyroid glands of male rats with different doses of BPA, also central vein dilation, enlarged nuclei, vacuolation of hepatocytes were observed in the liver of BPA treated groups and different degrees of histological changes include depression of spermatogenesis, decrease of leydig cells in dose dependent manner were found in testicular tissues of BPA treated groups.

Keywords

Bisphenol A --- thyroxin --- ( AST)


Article
RADIOLOGICAL & EVALUATION STUDY OF SOME MINERAL COMPONENT TO EFFECT OF MEDIAN NEUROECTOMY OF THE MID-SHAFT OF METACARPAL BONE IN FORE-LIMB IN DONKEYS

Loading...
Loading...
Abstract

The present study assigns the effect of neuroectomy to bone healing of experimentally induce bone fracture in 3rd metacarpal bone ,in donkeys by macroscopic, radiographic and evaluation of mineral levels(ca,p). Sixteen donkeys of male sex with the age 1.5- 2 years, weighting 125-150 kg. were used for this study. These animals were healthy clinically. The donkeys were divided randomly into two groups( non- neuroectomized and neuroectomized) eight donkeys each group. The animals anaesthetized with xylazine Hcl at dose 0.5 mg/kg. B.W., then injectable Ketamine Hcl at the dose 3 mg/kg B.W.which administered intravenously. The animals of non- neuroectomized group, induced one fracture at the mid of the shift of 3rd metacarpal bone. Evaluated with clinical signs macroscopical, estimation of level of mineral and radiographic examination. Gross examination swelling in the site of fracture and lameness. The callus formation shows in radiographic examination.The calcium concentration was highly significant increase after 30th and 60th days , which the phosphorus level increase after 30th days and then s decrease at 60th days

Keywords

neuroectomy --- Metacarpal --- callus.


Article
EFFECT OF DIFFERENT LEVELS OF TREATED ROUGHAGE AND UN-DEGRADED CONCENTRATE ON RUMEN PARAMETERS OF ARABI EWES

Loading...
Loading...
Abstract

The present study was conducted at the Animal Farm/ College of Agriculture/ University of Basrah during the period from 2/12/2012 to 2/3/2013. The study included 24 milking ewes aged around 2-4 years, having single lamb, closely lambing date and weighted 42 kg. After giving the ewes preliminary period of 10 days, they were distributed randomly and equally to six feeding groups. The first group was fed 60% concentrate and 40% roughages; soya bean meal was treated by formaldehyde. The second group was fed 60% concentrate and 40% roughages with untreated soya bean meal. The third group was fed 50% concentrate and 50% roughages with treated soya bean meal. The fourth group was fed 50% concentrate and 50% roughages with untreated soya bean meal. The fifth group was fed 40% concentrate and 60% roughages with treated soya bean meal. The sixth group was fed 40% concentrate and 60% roughages with untreated soya bean meal (control). The ration was given as 4% of live body weight. The concentrate consisted of 40% barley, 20% corn, 30% wheat bran, 7% soya bean meal, 1% salt and 2% Calcium bicarbonate. Roughage was wheat straw treated with 4% urea and 3kg/ton yeast. Treated soya bean meal reduced degradable protein from 70% to 60%. There were no significant differences among feeding groups in pH, total bacteria and cellolytic bacteria before feeding. However, the differences reached significant level (P<0.05) after 3 hours of feeding. The third group recorded highest pH value (6.82) and cellolytic bacteria (8.7x 106). The fifth and sixth groups showed highe significant improvement in total number of bacteria when compared with other groups (11.37x107 and 11.86x107).Propionic acid level and the percentage of acetic: propionic were significantly (P<0.05) influenced by different treatments, comperation with fivth and sixth treatment.

Keywords

Soya bean --- formaldehyde --- Ewe.


Article
HIGH EXPRESSION OF P53 PROTEIN IN TOXOPLASMOSIS AMONG WOMEN WITH SPONTANEOUS MISCARRIAGE IN BASRAH

Loading...
Loading...
Abstract

Recurrent abortion is a worldwide problem, with undefined causes. Apoptosis could play a major role in the process. The Objective of the work to detect the expression of p53 protein at the materno-fetal interface in patients with recurrent pregnancy loss (RPL). Immunohistochemistry analysis of P53 protein using paraffin embedded sections of curate samples obtained from 40 women divided into three groups : 16 women with recurrent abortion as postive with toxoplasmosis,10 women with recurrent abortion negative of toxoplasmosis and14 women with no histry of abortion as control group . The mean value of the expression of P53 protein was (40.87± 7.54),which is significantly higher than that of the second group(28.2± 4.89),and the third group (13.07± 4.49). The high expression of p53 protein in women with RPL may have a role in accelerating placental apoptosis leading to failure of pregnancy.


Article
EFFECT OF LOW INTENSITY PULSED ULTRASOUND ON REPAIR OF TENOTOMIZED ACHILLES TENDON OF RABBIT

Loading...
Loading...
Abstract

Tenotomized Achilles tendon Rabbits were used to investigate the effect of lowintensity pulsed ultrasound (LPUS) to accelerate the repair of injured tendon. The medial Achilles tendons of 18 local breed male rabbits were transected of all animals without suture, and bandaging was performed to immobilization the injured limb. Nine animals were chosen randomly to receive low-intensity pulsed ultrasound, the other 9 animals served as control. pulsating ultrasound with intensities 500 mW/cm2 and frequency 2.5 MHz were given over the healing tendons for 10 minute daily, using a gel as the coupling agent between the ultrasound probe and the skin. The healing tendons were evaluated clinically, macroscopically and microscopically for three periods at 7, 14 and 21 days respectively after tenotomy, three rabbits for each period. In the ultrasound-treated group at 14 and 21 days postoperatively, the macroscopic and histological studies demonstrated that the low-intensity pulsed ultrasound enhanced the healing of tendomize Achilles tendon. The ultrasound treated group showed more mature organization at all end points of injured tendons compared


Article
AMELIORATING AND PROTECTIVE ROLE OF QUERCETIN AGAINST O-ANISIDINE TOXICITY ON SOME REPRODUCTIVE ASPECTS OF LABORATORY MALE RATS (Rattus norvegicus).

Loading...
Loading...
Abstract

The present study assessed the effects of O-anisidine hydrochloride and the ameliorating effect of Quercetin dihydrate in laboratory rats. Sixteen male and thirty two female rats (Rattus norvegicus) were used and divided into eight equal groups of two male and four female rats each. The results revealed that the treatment with Oanisidine hydrochloride for 30 days (T1, T2 and T3 groups) caused significant decrease in the males body weights, sperm count, individual and massive sperm motility, testes weights, and epididymis weights as compared with control and (T4, T5, T6 and T7) groups at (P≤0.05).When Quercetin dihydrate was offered as an ameliorating agent, it showed a significant ameliorating effect by increasing the body weights, sperm count, individual and massive sperm motility, testes weights, and epididymis weights . When Quercetin dihydrate was offered alone in the ration of the sixth treated group (T6), it caused clear significant ameliorating effect on all sperm parameters comparing with all treated groups and the sperm count was even significantly higher than that of control group while the other aspects were similar to those of control group at (P≤0.05). Beside, O-anisidine caused significant decrease in the number of pregnant females, number of delivered litters, weight of litters and sex ratio, and it prevented the pregnancy from being occurred in the group where both male and females are treated with it (T1). When Quercetin was mixed with Oanisidine in the ration of (T7), it significantly ameliorated the pregnancy chances,number of litters and the sex ratio as compared with the other groups but it didn’t reach to significant level with control group at (P≤0.05).

Keywords

O-anisidine --- pregnancy --- Rats.


Article
STUDY THE PROTECTIVE ROLE OF QUERCETIN AGAINST O-ANISIDINE TOXICITY ON SOME HEMATOLOGICAL PARAMETERS OF LABORATORY MALE RATS (RATTUS NORVEGICUS).

Loading...
Loading...
Abstract

The study was designed to assess the protective role of Quercetin against Oanisidine toxicity. 24 male rats (Rattus norvegicus) were used and divided into 3 equal groups of 8 male rats each. The first group was the control group in which the animals were fed on a standard ration for 15 days, then they were terminated. The second group was fed on a ration contains 1000mg/kg O-anisidine hydrochloride for 15.The third group was fed on a ration contains 1000mg/kg O-anisidine hydrochloride + 80mg/kg Quercetin dihydrate for 15. The results revealed that the treatment with Oanisidine hydrochloride for 15 days (1rst treated group) caused significant decrease in the R.B.C. count, Hb concentration, P.C.V. percentage, neutrophil and lymphocyte counts and it caused significant increase in platelets count, total leukocytes, monocytes, eosinophil and basophil counts, as compared with control group. When Quercetin dihydrate was offered as a protective agent in the ration of the 2nd treated group, it showed a significant ameliorating effect by increasing the R.B.C. count, Hb concentration, P.C.V. percentage, neutrophil and lymphocyte counts and it caused significant decrease in platelets count, total leukocytes, monocytes, eosinophil and basophil counts, as compared with the 1rst treated group. For the blood indices (RDW, MCH, MCHC, MCV, and MPV) there were no significant differences among all the experiment groups except for the mean platelet volume (MPV), where Oanisidine hydrochloride caused significant decrease in the MPV of the 1rst treated group as compared with control and 2nd treated group at (P≤0.05).


Article
EVALUATION OF THE ISOLATION AND DETECTION METHODS FOR SALMONELLA SPP. FROM EGG SHELL CONTAMINATION USING MULTIPLEX PCR.

Loading...
Loading...
Abstract

A total of seventy four egg samples were collected from different markets sources, to investigated for Salmonellaspp. the bacteria was isolated and identified using culturing on selective media, in addition to, biochemical and serotyping by monovalent antisera. Multiplex Polymerase Chain Reaction(PCR) detection of theinvA; and DH genes was used for conformation of the identification of the Salmonella spp.. Twenty eight samples from seventy four eggs provide positive results with PCR as Salmonella spp. depending on InvA gene which is the target for the identification at the genus level.

Keywords

Egg shell --- invAgene --- Multiplex PCR


Article
MORPHOLOGICAL AND HISTOCHEMICAL STUDY OF THE EVENTS CYCLE OF SPERMATOGENESIS IN THE TESTES OF ADULT MALE COMMON QUAIL

Authors: Satar Abood Faris
Pages: 289-301
Loading...
Loading...
Abstract

Twenty adult common quail male obtained from Soq-Al-Shiyookh local market .They were kept under light and feeding conditions adopted by it. The anatomical study was appeared that the reproductive system in common quail consist of paired testes ,efferent ducts, epididymis, vasdeference and recetaculum .The testes are lying within the abdominal cavity and are joined to the dorsal body wall by short connective tissue . They are paired oval-shaped structure .The average of the left testicular size is ( 2 ± 0.04 cm3) and the average of the right testicular size is (1.85 ± 0.01 cm3).The average of the left testicular weight is (1.9 ± 0.01 gm. ) and the average of the right testicular weight is (1.7 ± 0.01 gm.). The testes of quail are long axis is oblique and directed dorso caudally .The epididymis is relatively large and is firmly attached to the dorso lateral surface of the testes. The histological study was showed that the spermatogonia are identified adjacent to the basement membrane of the seminiferous tubules either singly or in cluster but they do not form a complete basal layer. The primary spermatocyte are the largest cells of the spermotogenic population . Their nuclei are notable for both their size and appearance ,being big and vesicular is due to the presence of condensed chromatin. The secondary spermatocyte enter the second meiotic division ,the subsequent cells formed from this division are spermatids. Spermatocytogenesis ends when spermatids appears .The spermatids undergo a complex process of differentiation leading to the transformation of these spermatids into sperm . Based on changes in the acrosomes and nuclei during the development of spermatid will give rise mature spermatozoa

Keywords

Quail --- Epididymis --- Spermatogensis.


Article
INVESTIGATION OF INFECTIOUS LARYNGEOTRACHEITIS VIRUS IN IRAQI CHICKEN FARMS

Loading...
Loading...
Abstract

Twenty samples of Laryngeal and Tracheal tissues from laying chickens of two flocks with suspicious infectious laryngeotracheitis were tested for the detection of the causative virus by using virus isolation in chicken embryo fibroblast (CEF)cell culture and chicken embryos by chorioallantoic membrane inoculation and conventional polymerase chain reaction(PCR).The virus was isolated from three samples (larynx and trachea) out of ten samples at Al-Sawara city on (CEF)cell culture and produce pock lesions on chorioallantoic membrane of infected chicken embryos in which the virus was isolated. Positive PCR results were detected in the three clinical samples, isolated virus in CEF cell culture and isolated virus in chicken embryos (inoculated on chorioallantoic membrane).


Article
COMPARATIVE STUDY BETWEEN NIGELLA SATIVA AND ZIZIPHUS SPINA-CHRISTI EFFECTIVENESS ON SKIN A SUPERFICIAL BURN HEALING IN RABBITS.

Authors: Luay A.Naeem
Pages: 311-319
Loading...
Loading...
Abstract

The present study to evaluate the third degree burn involved all skin layers (full-thickness burns), and that by using ziziphus leaf extract and nigella seed extract as an ointment. Healthy twelve mature rabbits either sex, weighing 2-2.5 kg, bred locally were used. Divided in to two groups (ziziphus group and nigella group) six rabbits of rabbits each one burned experimentally by using fire source directly on skin surface and then treated with local application of ointment (ziziphus leaf ointment and nigella sativa seed ointment) each one on it is group with daily dressing by using medical gauze. This study appeared the benefit effect of ziziphus ointment and nigella ointment in burn healing in histopathological changes, but the group treated with ziziphus leaf extract (ointment) was better than the other group in cellular response to inflammatory process that occur during healing by early apparition of re-epithelialization

Keywords

Table of content: volume:14 issue:1