Table of content

Basrah Journal of Veterinary Research.

مجلة البصرة للابحاث البيطرية

ISSN: Print:18138497 E; 24108456
Publisher: Basrah University
Faculty: Veterinary Medicine
Language: English

This journal is Open Access

About

. Basrah Journal of Veterinary Research (Bas.J.Vet.Res.) is the official journal of College of Veterinary Medicine, University of Basrah, Iraq. The Journal is a biannual international peer-reviewed, open access research publication covering all aspects of veterinary science. The journal publishes original papers in English, case reports and reviews relevant to any area of veterinary sciences and veterinary medicine.

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Mobile;07801034925
E.mail;alaasawad24@gmail.com

Table of content: 2016 volume:15 issue:1

Article
Some observations on the spermatogenesis in the testes of rabbit
بعض الملاحظات عن نشأة الخلايا النطفية في خصي الارنب

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The seminiferous tubules of the testes of rabbits were lined by multilayered germ cells . The first layer was occupied by the spermatogonia , which were differentiated into type A(dusty type) spermatogonia, Intermediate type and type B (crusty type) spermatogonia. Pictures of Preleptotene, Leptotene, Zygotene ,Pachytene, Diplotene and Diakinesis. Primary spermatocytes were found and followed by secondary spermatocytes. Reading the morphological changes, the spermatid proposed 10 stages of cellular association during the cycle of the seminiferous epithelium in rabbit .


Article
HISTOMORPHOLOGICAL STUDY OF HEMAL LYMPH NODE OF THE CAMEL(Camelus dromedarius)
دراسة نسجية شكليائية للعقدة اللمفية الدموية في الجمل

Authors: ِA.M.Hussin
Pages: 7-12
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The study was carried out to investigate the histomorphology of hemal lymph nodes of camel (Camelus dromedarius) . The results showed that the hemal lymph node was conical in shape with convex wide base and narrow apex. It was encapsulated by a thick fibrous connective tissue capsule from which long trabeculae emerge. The stroma of hemal lymph node consists of randomly distributed lymphatic nodules ,lymphatic cords and hemal sinuses. Presence of erythrocytes, leukocytes especially lymphocytes ,monocytes, plasma cells, macrophages and megakaryocytes were seen . Megakaryocytes or giant cells possess an irregularly lobulated nuclei with course chromatin and unapparent nucleoli.

Keywords

camel --- sinuses --- lymph node


Article
IMMUNE RESPONSE OF BALB/C MICE AGAINST GENETIC VACCINATION WITH LEISHMANIA MAJOR GP63 GENE (LMAJGP63)
الاستجابة المناعية لفئرانBalb/c ضد التطعيم الجيني بجين Lieshmania major gp63 (lmajgp63)

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Leishmania major glycoprotein 63 (lmajgp63) gene was used in this study as DNA vaccine candidates. Gene was inserted into VR1012 plasmid by using standard molecular biology protocols, resulting in preparation of lmajgp63/VR1012 plasmid. Vaccine either used as naked or gold particles coated DNA vaccine in immunization of females Balb/c mice. Animals were immunized at week 0, 2 weeks and 6 weeks. Dermojet needle free injector had been used to deliver gold particles coated DNA vaccine intradermally (I/D) while ordinary needle injection was used to deliver naked vaccine intramuscularly (I/M). Immune response for each vaccinated group were detected, two weeks after the third administration of the vaccines, by estimation of serum concentration of IL-2, IL-4, IL-10 and INF-γ, as well as anti-soluble Leismania antigen (anti-SLA) IgG titer, by ELISA test. The results demonstrated the effectiveness of DNA vaccines in induction immune response comparing to control groups (P<0.05). The highest serum concentrations of IL-2, INF-γ and anti-SLA IgG OD value observed in mice group which immunized with gold particles coated vaccine injected I/D (182.10 pg/ml, 1699.20 pg/ml and 0.6101 respectively), while the lowest titer was observed in group vaccinated with naked plasmids injected I/M (103.60 pg/ml, 1183.20 pg/ml and 0.3395 respectively). On the other hand group treated with naked plasmid I/M shows the highest titer of IL-4 and IL-10 concentration (85.30 pg/ml and 53.10 pg/ml respectively), while the lowest titer observed in group injected I/D by gold coated plasmid (50.90 pg/ml and 41.88 pg/ml respectively). The results also demonstrated that I/D injection of gold particles coated DNA vaccine induced the highest Th1/Th2 response [IL-2/IL-10 (4.348) and INF-γ/IL-4 (33.383)], while I/M injection of naked vaccine gave the lowest results [IL-2/IL-10 (1.951) and INF-γ/IL-4 (13.879)].


Article
EFFECTS OF USING FISH BIOSILAGE AS FISH MEAL REPLACER ON FEEDING, GROWTH AND GUT HISTOLOGY IN COMMON CARP Cyprinus carpio L. FINGERLINGS

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This study was carried out to evaluate the effects of locally produced fish biosilage as fish meal alternative on feeding, growth efficiency and gut histology in common carp C. Carpio fingerlings. Biosilage was prepared by fermenting marine by-catch fish with date fruit residues, domestic vinegar and citric acid. The produced biosilage was incorporated in feeds to replace 0, 25, 50 or 75% of fish meal protein. Fish were fed for 14 weeks and feeding and growth parameters were close in the four feed groups so as fish survival rate during the experiment (88.9-93.3%). Histological examination of intestine and liver sections has showed improvements when fish fed on the four different feeds. Initial fish group showed signs of nutritional deficiency through limited size of intestinal villi and hepatocytes. However, the histological structure of gut was improved after fish were fed on the experimental feeds without significant differences between fish meal or fish biosilage feeds. The study concluded that fish silage could replace fish meal without adverse effects on feeding, growth efficiency and gut histology.

Keywords

; Biosilage --- fingerlings --- protein.


Article
TOXICO EPIDEMIOLOGY OF LEAD, NICKEL AND CADMIUM LEVELS IN SHEEP SERUM OF AL BASRAH PROVINCE
قياس تراكيز الرصاص و النيكل والكادميوم لمصل ذكور الاغنام في مناطق مختلفة من محافظة البصرة

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The comparison toxico-epidemiological study designed to evaluate the Lead, Nickel and Cadmium levels in healthy sheep of different regions in Al Basrah province and compared with sheep from Animal Farm of Agriculture College /University of Basrah(as control group). Sixty three adult male sheep were tested for the study (6 animals for each region). Blood samples were collected from each ram, the Pb, Ni and Cd values were measured. AST, ALT and creatinine values were also determined. The results revealed to high increase levels of Cd, and Pb concentration among the different regions and compared with control rams values. There were no significant differences in the transaminase enzymes activity and creatinine concentration among the animals tested. In spite of the rise in trace minerals values but it did not threshold to harmful effect on rams’ body physiology.

Keywords

Nickel --- Creatinine --- Sheep


Article
HISTOLOGICAL STUDY OF THE EFFECT OF AQUEOUS EXTRACTION OF THE CASTOR SEEDS ON THE INTERNAL ORGANS IN MALE WHITE MICE
دراسة نسيجية لمعرفة تاثير المستخلص المائي لبذور الخروع على الاعضاء الداخلية للفئران البيضاء

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Water extract of castor bean was prepared and injected in mice in order to study the effect of acute phase of toxicity (in different doses) on living tissue, for these reasons, 24 male white mice were divided equally into 4 groups. The 1st, 2nd, and 3rd groups were injected with the aqueous extraction of the castor seeds subcutaneously with 20, 30 and 60 mg/Kg of body weight, respectively, while the 4th group used as control group. After 24 hrs. the internal organs showed degenerative changes and proteintious material in the spleen and kidney, also these organs in addition to liver and stomach showed inflammatory reaction in their parenchyma. The lesions in the examined internal organs were mild in a dose of 20 mg/Kg B.W. and more sever lesions seen in a dose of 60 mg/kg of body weight.

Keywords

Castor seeds --- spleen --- Lesions.


Article
STUDY THE ANTIOXIDANT EFFECT OF TOMATO EXTRACT IN OXIDATIVE STRESSED RATS.
دراسة التأثير المضاد للتاكسد لمستخلص الطماطم ضد الإجهاد ألتأكسدي في الجرذان

Authors: Haider H. Humaish
Pages: 66-80
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Tomato, is today the most popular garden vegetable in worldwide, because of its high consumption. Tomato contain a variety of phytochemical , such as lycopene , flavonoids, glycosides and other chemical constituents that have been beneficial protective effect. The present study carried out to evaluate the effect of two different doses (2 mg/kg BWand 4 mg/kg BW) of tomato extract against hydrogen peroxide-induced oxidative stress in albino rats. Thirty two adult male albino rats randomly divided into four equal groups were used in this study, the results revealed that administration of 0.75% H2O2 in drinking water (groupI) produced significant decline of antioxidant enzymes ( superoxide dismutase (SOD) , glutathione (GSH), catalase (CAT) ) and serum albumin concentration , with significant elevation of lipid peroxidation rate by estimation of malondialdehyde (MDA) and peroxynitrite radical (ONOO). Also, H2O2 caused significant increase in serum concentration of alanine amino transferase (ALT), aspartate amino transferase (AST), alkaline phosphatase (ALP), and bilirubin . On the other hand the animals treated with H2O2 plus 4 mg/kg BW of tomato extract (groupIII) showed significant increase of SOD, GSH, CATand albumin with significant reduction of MDA ,ONOO, ALT, AST, ALP, and bilirubin comparing with control group. Depending on the above oxidant and antioxidant markers, it seems that 4 mg/kg BW. of tomato extract exert beneficial action protect against H2O2 induced oxidative stress in rats.

Keywords

Oxidative stress --- GSH --- Tomato extract --- ALT


Article
CLINICAL AND LABORATORY EVALUATION OF VITAMIN A AND BETA CAROTENE IN LOCAL SHEEP BREED OF BASRA, BASRA_ IRAQ
التقييم السريري والمختبري لنقص فيتامين (ا) و البيتا كاروتين في الضأن المحلي لمحافظة البصرة ,البصرة, العــــــراق

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The study was conducted on (96) local sheep breeds of different ages and both gender reared in Basrah, Iraq. Suspected animals show signs of night blindness, abortion of pregnant ewes, diarrhea with passing of normal small fecal materials ,nervous sings (such as paralysis of skeletal muscles, blindness due to constriction of the optic nerve canal, convulsions encephalopathy). Moreover diseased animals were also show panting and changing of skin .The levels of vitamin A and Beta carotene were estimated by ELISA test and results showed that local sheep suffer from hypo vitaminosis A with mean level of( 1.8 nmol/l). However according to regions of Basrah deficiency of vitamin A and carotenes respectively were indicated of center of Basra (1.7nmol/land 11.2 ng/ml). AlQurna (1.7 nmol/l and 15.9 ng/ml) .Azzubair (2.1 nmol/l and 26.3 ng/ml). Shateelarab (1.2nmol/l and 17.7 ng/ml).and Abulkhaseeb (2.0 nmol/l and 16.8ng/ml) It have been concluded that local sheep breeds of Basra province were suffer from hypovitaminosis A, therefore animals reared in those area should be screened periodically.

Keywords

Sheep --- Skeletal Muscles --- Diarrhea


Article
HISTOPATHOLOGICAL STUDY OF THE DISTAL THIRD FRACTURES OF RADIUS IN DOGS, TREATED WITH LLLT,
دراسة مرضية نسجية لكسور الثلث القاصي من عظم الكعبرة في الكلاب المعالجة بالليزر واطئ الطاقة

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The aim of study was a histopathological evaluation of the effects of low-level laser therapy (LLLT) such as diode laser on healing fracture of distal third of the radius in dogs, at the end of 1st,3rd and 6th week post operation .18 male adult street dogs were used to obtained transverse fracture at the distal third of the radius under highly aseptic technique, the anesthesia was done by using the combination of (15 mg/Kg.B.W ) ketamine hydrochloride,and (5 mg/Kg.B.W ) xylazine, the fractured limb fixed with (Gypson) as an external fixation devise, and window is obtained at the anterior surface of the radius at the fracture sites for direct irradiation of the laser. The experimental animals were divided into two equal groups each contain 9 dogs, the control group lift without any irradiation, while the treatment groups received daily single dose of laser (850 nm, for 5 minutes at 72 hours interval for 14th days). Histopathological finding at the end of the 1st week revealed early formation of irregular trabecular bone (immature woven bone), compare with the control group at the same period, then these trabecular bone became later more regular, dense and width at the end of the 3rd week in the treatment group, then quickly changed to lamellar bone in the end of the 6th wk P.O. in the treatment group compare with the control group. It concluded that The LLLT promote and accelerate fracture healing, in the distal third of the radius in dogs

Keywords

LLLT --- Radius fracture --- Diode laser


Article
PASSIVE EFFECT OF X-RAY IRRADIATION ONTESTICULAR FUNCTION,SPERMATOGENESIS, SOME BLOOD PARAMETERS AND TESTOSTERONE IN MALE RABBITS
التأثير السلبي لإشعاع الأشعة السينية على وظائف الخصية وتكوين النطف, وبعض مكونات الدم والهرمون الذكري في ذكور الأرانب

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The present study was done to determine and evaluate the effect of X-ray irradiation on the testicular tissue of rabbits those were exposed for a long time. Ten male rabbits, 8-9 months old and their weight approximately two kg. Rabbits were exposed to X-ray irradiation for two months/ twice daily. Blood parameters and testosterone hormone were measured within 20th, 40th, and 60th days after exposure. Orchictomy were done by surgical methods after 60th days for histopathological examination. The results revealed highly changes in testis such as atrophy, hyper atrophy, blood vessel congestion and suppression of spermatogenesis, blood parameter also changed and testosterone levels reach to zero at 60th days after exposure. In concluding that the persistence of X-ray exposure caused deterioration and passive effects on testicular tissue and other organs of rabbits.

Keywords

X-ray --- Irradiation --- Rabbits


Article
SILICON POLYMER FOR CAST OF PARANASAL SINUSES OF IRAQI LOCAL GOAT (Capra hircus)
استخدام بوليمر السليكون لدراسة الجيوب الجنب الأنفية في الماعز العراقي المحلي

Authors: Dhuha Adel Kareem , Alaa A.Sawad
Pages: 111-118
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The present work is designed to study the anatomical features of the paranasal sinus in local goat(Capra hircus) to distinguish and describe the types of the paranasal sinuses. Five heads of local goat were collected are used in this study , Used of silicone casts and imaging in order to study the shape, position of sinuses . The study revealed that the paranasal sinuses are ventilated spaces connected to the nasal cavity. They develop as blind ending pouches between the lamina of the bones of the skull or are a series of cavities arranged one after another like pyramidal shapes consists of five sinuses. which cavities are frontal , maxillary, lacrimal, palatine, conchal sinuses with absence of sphenoid sinus . The frontal sinus lies above the eyes (above the orbits) and have two types larger lateral and smaller medial, Maxillary sinus is large and located under the cheeks, while the lacrimal sinus is very small located in the dorsal part of the lacrimal bone . The palatine sinus is located in the hard palate. There are three conchal sinuses located inside three conchae of nasal cavity. The dorsal, ventral ,middle conchal sinuses located inside dorsal ,ventral ,middle nasal concha respectively , the dorsal conchal sinus longer than ventral and middle conchal sinuses. The aim of study to identify the morphological features of the paranasal sinuses and relation between them and with other regions. in the Iraqi local goat because veterinary importance and its effect on public health. INTRODUCTION Goats are a popular large animal model because they can be easily obtained and bred. Over the past decades, they have been used to study immunoserology, microbiology, biological products, gene transfer and tissue-engineering repair of bony defects and for teaching and biomedical research and for surgical training.( 1, 2). The paranasal sinuses of goat are a series of cavities arranged one after another. These cavities are frontal , maxillary, lacrimal,palatine, conchal sinuses. The Frontal sinus, Consists of spaces within the bones between the cranial cavity and the nasal cavity divided into two parts, smaller medially and larger laterally ,while the Maxillary sinus, are the largest sinus is divided by the infraorbital canal into medial and lateral parts. The lateral partion is larger and extends caudally into the lacrimal bulla; while the medial portion located medial to the infraorbital canal , is continuous with the palatine sinus ;Lacrimal sinus are relatively small and lies near the frontolacrimal junction, The Palatine sinus Communicate with the maxillary sinus through a wide maxillopalatine opening and three conchal sinuses (dorsal, middle, ventral) formed by concha of nasal cavity ( 3,5). This sinuses are cavities lies in the interior at some bones of the skull .the walls of the sinuses are composed of compact bone and are lined by a muco- periosteum (muco-endosteum) which is continuous with the nasal cavity( 3,8). Anatomical features of the paranasal sinuses and relation between them and with other regions in the Iraqi local goat were aimed in this study.

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Article
DIAGNOSIS OF BOVINE ANAPLASMA MARGINALE IN NORTH WESTERN LIBYA USING SEROLOGY AND BLOOD FILM EXAMINATION: A COMPARATIVE STUDY
تشخيص اصابة الابقار بجرثومة Anaplasma marginale في شمال غرب ليبيا باستخدام فحص المسحات الدموية والفحوصات المصليه : دراسة مقارنة

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Anaplasmamarginale (A. marginale) is an obligate intra-erythrocytic rickettsia;it is the cause of anaplasmosis, an important tick-borne disease of cattle. Recovered and vaccinated cattle in endemic areas are apparently normal but remain persistently infected and serve as reservoirs for the parasite.This study intended to detect A. marginale in infected and apparently healthy subclinically infected cattle in North Western Libya. During the period extended from March-2006 till September-2007, blood samples and blood smears were collected from totally 119 adult cow (group – I). These cows were raised at some governmental and private farms in Tripoli, Al-Zawiya and Imssallata districts. Blood smears were stained with May-Grunewald- Giemsa stain and examined under Light microscope to detect the presence of intraerythrocytic bacteria. Indirect- ELISA (IELISA) using a 19 KD A. marginale recombinant antigen was used to detect serologically positive reactors.During the study period, 20 cases of acute anaplasmosis were diagnosed in these farms (Group-II); where, three cows died and two aborted. The Seroprevalence for A. marginale by IELISAwas 64% and 100% in group I and II, respectively. Stained thin blood smears failed to detect infective RBCs in group I, however, variable degrees of parasitaemia were detected in group II. In conclusion, this study approved that serological test (IELISA) was more reliable than direct microscopic examination of stained blood smear in detection of chronic persistent anaplasma-infected cows in endemic areas.


Article
STUDY THE EFFECT OF PROANTHOCYANIDIN AND RANITIDINE ON HAEMATOLOGY AND BIOCHEMICAL PARAMETERS IN ADULT FEMALE RABBITS WITH GASTRIC ULCERATION INDUCED BY INDOMETHACIN
دراسة تأثير بروانثوسياندين و الرانتدين على المعايير الدمويه والكيموحيويه في اناث الارانب البالغة المستحدثه القرحه بواسطه الاندوميثاسين

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The study was conducted in Collage of Veterinary Medicine Basrah University,to evaluate the effect of proanthocyanidin and ranitidin on gastric ulcer, haematological and biochemical parameters changes by using female rabbits with acute gastric lesions induced by indomethacin.The study done on (30)adult female rabbits, their weight ranged between (1500-2000.0mg); divided into five groups, each group consist of six rabbits as the following: Group1:- healthy (negative control group) administrated normal saline (0.9 of normal saline) for 10 days; Group 2:-given indomethacin 75mgkg B.W. for two days(positive control group); Group 3:- at first given indomethacin 75mgkg B.W. for two days, then treated with proanthocyanidin(PA) 100mgkg B.W. for 10 days; Group 4, initially given indomethacin 75mgkg for two days, then treated with proanthocyanidin(PA) 200mgkg for 10 days; Group 5, given indomethacin 75mgkg for two days, then treated with ranitidin 50mgkg for10 days.The results showed that proanthocyanidin(PA) and ranitidin caused significant reduction (P≤0.05) in gastric volume, ulcer area, serum MDA, gastric tissue MDA while significant increase (P≤0.05) in mucin and gastric pH. It also revealed significant decrease (P≤0.05) in glucose concentration in rabbits treated with proanthocyanidin compared to positive control group while showedno-significant change in glucose concentration in rabbits treated with ranitidine compared with positive control group. It also, showed significant increase (P≤0.05) in Red Blood Cell(RBC),Hemoglobin(Hb) andMean Corpuscle hemoglobin concentration(MCHC) in rabbit treated with proanthocyanidin or ranitidin, while there was significant decrease (P≤0.05) in Mean Corpuscle Volume(MCV) in rabbits treated with proanthocyanidin in dose of 100mgkgand ranitidine in a dose of 50mg/kg with non-significant change of MCV in female rabbits with gastric ulceration treated with proanthocyanidin at dose 200 compared with positive control group.It showed non-significant changes in White Blood Cell (WBC) of female rabbits with gastric ulceration treated with proanthocyanidin compared with positive and negative control groups, while the results showed significant decrease (P≤0.05) in WBC of female rabbits with gastric ulceration treated with ranitidine group compared with positive and negative control groups and the other groups. The study revealed significant decrease (P≤0.05) in total cholesterol, triglyceride, Low Density Lipoprotein(LDL) and very Low Density Lipoprotein(VLDL) of female rabbits with gastric ulceration treated with proanthocyanidin and ranitidine group compared with positive control group while it showed significant increase (P≤0.05) in High Density Lipoprotein (HDL) in rabbits treated with proanthocyanidin and ranitidine group compared with positive control group. It is concluded that proanthocyanidin extract of the grape seeds(Vitis vinifera)displayed good antiulcer activity, hypoglycemia effect, amelioration of heamatological parameters and improve dyslipidemia corroborating the folk use of Vitis vinifera preparations, and contributing for its pharmacological validation.


Article
PCR BASED DETECTION OF GRAM NEGATIVE PSYCHROTROPHIC BACTERIA IN COWS RAW MILK
الكشف المعتمد على تفاعل سلسلة البلمرة للبكتريا السالبة لصبغة غرام المحبه للبروده في الحليب الخام للأبقار

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Investigations of the proteolytic Gram Negative Psychrotrophs(GNP) bacteria was the basal objective of this study. A conventional diagnostic PCR technique based on three pairs of primers including SerAgeneto amplify an 950 bp fragment of Acinetobacter sppDNA,serAgene for/A. hydrophila ( 650bp): and aprgene for/S. marcescens (500bps) was done.In the present study the 29 bacterial isolates obtained from 100 cows raw milk samples were collected randomly from healthy cows with different age and breed present in different farms of Thi-Qar province, previously refrigerated for 72 hr. These isolates subjected to cultural-based enrichment and PCR- based identification .The present results revealed that the raw milk GNP contamination overall ratio was 29% . Acinetobacter spp were the most predominant bacteria (16%) among the studied GNP contaminants, while A.hydrophila appeared in a ratio of 7% and S. marcescens showed the lower ratio ( 6%). ,the results of the studied genes product of GNP bacteria was considered to be highly statistically significant (P>0.001).The distribution of studied GNP according to age ,parturition number and breed of studied animals was investigated. The effect of these factors on the PCR-based identification results was considered to be not statistically significant(P>0.05) however, the higher overall ratio(29.1%) for cow raw milk contamination was observed in raw milk of cows between <3 - < 9 year of age. In general cows at first age group (<3 - < 9 year) showed high ratio of raw milk contamination(7.6 and6.3%) with GNP bacteria (Serratia marscense and Aremonase hydrophila respectively)..Concerning the number of parturition, cows with high numbers of parturition( ˃ 6-<12) showed high overall ratio(38.5%) of contamination also high ratio of Acinetobacter spp(23.1%) ,Aremonase hydrophila(7.7%) and Serratia marscense (7.7%) were observed in the same group of cows. According to breed, a high overall ratio of GNP bacterial contamination was observed in 40% of crossbred cow raw milk followed by Friesian cows(32.1%).Beside that raw milk of crossbred cows showed a high ratio of contamination with Acinetobacter spp (20%) and Aremonase hydrophila(13.3%) while Serratia marscense appeared as a higher contaminant in Friesian cows raw milk with the ratio of its contamination was10.7%.

Keywords

Raw milk --- Gram negative --- GNP .


Article
PRE HATCHING DEVELOPMENTAL HISTOLOGICAL CHANGES OF THE TESTISIN MALLARD DUCK (ANAS PLATYRHYNCHOS)
التطورالنسجي لفترات ماقبل الفقس لخصية البط المحلي (Anas platyrhynchos)

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The current study was aimed to investigate the development of testis during pre-hatching periods to diverse the components of the testis in duck embryo from embryonic day 5 to 19 consequently; fifteen males' of mallard ducks embryos have been used. The results showed that development of testis has divided into two periods: the first one was the period of undifferentiated gonad (genital ridge) while the second period was the development into a testis which showed various stages. The undifferentiated stage has revealed of development a small protruded thickening of germinal epithelium and primordial germ cells on the ventromedial surface of the mesonephros and dorsal mesentery.The development periods has showed three stages: development of rete cords, sexual cords,primary somniferous tubules and Sertoli progenitor cells at the 8-10th day embryos. During 14th day old embryo was revealed of development immature somniferous tubule, tunica albuginea and testicular capsule. While the period of 19th day embryo showed that development of interstitial cells and thin septa from tunica albuginea, .in addition to marked division of spermatogonia cells. Size of left testis was larger than the right one also the epithelial layer of left gonad is thicker than that covering the right gonad.


Article
STUDY OF AMERICAN COCKROACH ALLERGY DISTRIBUTION AND ITS ASSOCIATION WITH SOME HLA- DQ AND - DR ALLELES IN ATOPIC PATIENTS IN BASRAH PROVINCE / IRAQ
دراسة انتشار ارجية الصرصرالامريكي وارتباطها مع بعض الألائلHLA- DQ و – DRلمرضى الحساسية في محافظة البصرة / العراق

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Ninety six for each healthy(n=96)and atopic,(n=96)individuals duals from the same geographical region, paired by sex and age, their sera specific IgE antibodies were estimated by enzyme linked immune sorbent assay test (ELISA) and genotyped by polymerase chain reaction based onHLA-DQB1*0602, HLA-DQB1*0604 andHLA-DRB1*12. The specific IgE based on ELISA results revealed that Out of 96 only59 (61.5%) of atopic patients were sensitive to CR allergen. The association between sensitivity to CR allergen and age was considered to be not statistically significant (P>0.05).However the higher rate of CR allergens sensitivity(62.9%) was observed in first age group(<45 year) of atopic patients. In contrast the effect of sex on sensitivity to CR allergens was considered to be statistically significant (p<0.05) and the higher rate of sensitivity(75.6%) was observed in atopic patients males. The overall differences in the HLA-DQB1*0602, HLA-DQB1*0604 and HLA- DRB1*12 alleles frequency between patients and controlswerestatistically (p<0.05).According to the results of risk factors statistical analysis values(p:value = 0.0001; OR:84.3481; CI 95%: 5.065- 1404.520) HLA-DQB1*0602 allele was associated with susceptibility of patients to the CR allergy. The overall differences in the HLA-DQB1*0602, HLA-DQB1*0604 and, HLA- DRB1*12 alleles distribution between atopic patients and controls were statistically highly significant (p<0.01) concerning the effect of the age and sex. In general the allele HLA- DRB1*12 was not observed in both atopic patients and controls in contrast HLA-DQB1*0602 was present in atopic patients only while HLA-DQB1*0604 appeared in both patients and controls with different frequencies The older atopic patients showed higher frequency (61.8% ) for the HLA- DQB1*0602 allele . In contrast higher frequency of HLA- DQB1*0604 allele occurred in younger patients(40.3%) . According to sex ,the higher frequency of HLA-QB1*0602allele was observed in males patients(31.7%)while the allele HLA-DQB1*0604 higher frequency(17.9 %)was observed in the females of the control group. The overall frequency of HLA-DQB1*0602(60.4%)orHLA- DQB1*0604(39.6%) as a single allele was observed in the seropositive or seronegative atopic patients. The seropositive showed higher frequency(35.6 and 15.3%) for HLA- DQB1*0602 and HLA- DQB1*0604 respectively

Keywords

(ELISA) --- IgE antibodies --- Allergy.


Article
EVALUATION EFFECTS OF DIFFERENT CONCENTRATIONS OF AZADIRACHTAINDICA(NEEM) SEEDS OIL AS A BOTANICAL PESTICIDE TO ELIMINATE THE PARASITE OF Hyalomma spp IN COWS
تقييم تأثير التراكيز المختلفة للزيت المستخلص من بذور نبات Azadirachtaindica (النيم) كمبيد نباتي للقضاء على الطفيلي HyalommaSppفي الأبقار

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Abstract

The current study was conducted on extracted Azadirachtaindicaneem seeds oil using the ethanol, it was measured effectiveness inhibitory of neem seeds oil extraction in different concentrations of 0.001 and 0.003 unit/ml compared with commercial neem oil, known (Azadirachtin) concentration of 0.002 unit/ml on the Hayalomma Spp. The study was conducted on six calves were selected randomly with different ages ranging from 4-6 months in the veterinary clinic in Baghdad / Diyala bridge area. The animals were divided into three group with 2 animals each, namely control group that treated with 0.002 neem oil (Azadirachtin 3.000 ppm) and treatment groups 1 and 2 were treated with neem oil with concentration of 0.0001 and 0.0003 respectively. The parasitic infestation of Hayalomma Spp was confirmed in the laboratory diagnosis and the presence of ticks in addition to the clinical symptoms of infected calves.The objective of this study was to evaluate the effects of different concentrations of seeds oil of AzadirachtaIndica(Neem) on Hyalomma Spp eliminate on the Cows. Results showed high efficiency of neem oil concentration 0.003 unit/ml as an anti-ticks, where there was a decline of the number of parasites since of the first day to the fourth day of the treatment that showed the mean number of parasites isolated 18.0, 15.8, 3.5 and 0.0respectively, compared with concentration of 0.001 and control group with significantly reduced of the parasites number P< 0.004at 0.05. Also, the most important result showed that there was no side effects of the high concertation of 0.003 of neem oil to treat the infected animals. In conclusion, the concentration 0.003 of neem showed the best extraction to eliminate the parasites without effects of animals’health status.

Keywords

AzadirachtaIndica --- Neem --- Pesticide --- Hyalomma


Article
SEROEPIDEMIOLOGICAL STUDY OF OVINE Q FEVER IN BASRA PROVINCE ,IRAQ
. دراسة وبائية مصلية للتحري عن حمى كيو في الضأن في محافظة البصرة,العراق

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Indirect ELISA test was used to detect specific antibodies against Coxiella burnetti in sheep in different regions of Basra province.184 local sheep breeds were randomly selected of different ages and of both sexes were used in this study, including 98 pregnant ewes, 55 non-pregnant ewes, 8 aborted ewes ,7 ewes of still births, moreover 112 lower than 2 years,more than 2 years 72and 13 rams. Results showed, that the percentage of infection detected by indirect ELISA were 41.84%. It have been concluded that C.burnetti were infected ewes of different status in Basra province, therefor periodic investigation of suspected animals must adviced. were a significant between, areas, age, groups pregnancy status and still birth ewes .incontrast non significant different were detected among gender , abortion .

Keywords

ELISA test --- ELISA test --- Sheep


Article
EVALUATION OF SOME HEMATOLOGICAL PARAMETERS AND CLINICAL SIGNS AFTER REPEATED EXPOSURE TO WARFARIN IN DOGS
تقيم العلامات السريريه وبعض القيم الدمويه بعد التعرض المتكرر للوارفرين في الكلاب

Authors: Israa Abdul Wadood Muhammad Ali
Pages: 249-259
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Warfarin poisoning in dogs is not unusual which is used as a rodenticide. Competitive inhibition of vitamin K with an incomplete synthesis of the coagulation factors II, VII, IX and X can lead to a significant bleeding tendency. The study was conducted at college of veterinary medicine / Basrah university to Evaluate clinical ,hematological and clotting indices in dogs in case of warfarin poisoning, which include twelve dogs of both sex at age about three years old. The animals divided to three groups equally .first group treated with 3mg warfarin tablet given orally daily,the second group treated with 5mg until the signs of poisoning appears While the third group untreated as a control. The results showed that the first group exhibited signs of warfarin poisoning like hematuria, vaginal bleeding, severe eye congestion, limping, bleeding in toe, excessive salivation, severe pale of mucus membrane in gum, hemoptysis, also the second group exhibited signs of warfarin poisoning after ten days the signs was hematuria and vaginal bleeding ,the animal showed signs of severe eye congestion, depression, weakness, and lameness ,bleeding in toe and then excessive salivation ,sever pale mucous membrane in gum, hemoptysis, blood vomit. weakness, excitement,nose bleeding, eye bleeding,protruded of eye,congestion of gum, melena and incoordination. there is significant decrease (P<0.01) in mean value of red blood cell count in dog treated with 3mg warfarin (4.09 ×106 ± o.254) as well as hemoglobin level( 9.613 g/dl±o.6085 ), packed cell volume (23.133 ±0.592) . and there is increase in mean of MCV (72. 40 fl±1.29) , MCH (23.86pg±0.52) and MCHC (32.78 g/dl ±0.50 ) which indicate macrocytic anemia . Also dogs treated with 5mg warfarin showed that the mean of red blood cell count was 3.81 ×106± 0.2347, mean of Hb was 8.30 ±1.006 g/dl and PCV 28± 2.510 with significant decrease (P<0.01) in these parameters and there is significant increase in mean of MCV,MCH. and MCHC 77.07fl±1.31 , 22.74 pg±0.49, 31.38 g/dl ±0.294 respectively . There is significant increase(P<0.01) in mean of prothrombin time and activated partial Thromboplastin Time in dogs treated with 3mg of warfarin, 1.336 ± 0.146 , 1.036 ± 0.1074 min.Respectively whencompared with control animals 0.601± 0.0863 , 0 .153±0.003min. and There is significant decrease (P>0.05) in mean value of platelets count 253.80 × 103g/l ± 18.31 compared with control group 448.12 ±52.24 . ,also there is significant increase(P<0.01) in PT. and APTT. when treated with 5mg 1.855 ±0.2039 , 1.401 ±0.1051 min.respectively,whereas the mean of platelet 240.40 × 103 g/l ± 5.39 with significant differences(P<0.01) .


Article
HISTO-MORPHOMETRIC AND HISTOCHEMICAL COMPARATIVE STUDY OF THE LIVER IN COLLARD DOVE (Frivaldszky), RUDDY SHELDUCK (Pallas) IN SOUTH IRAQ.
دراسه شكلائيه نسيجيه قياسيه وكيمياء نسيجيه مقارنه للكبد لطير الفاخته والبط الصيني في جنوب العراق

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Abstract

The results of present study showed that the liver of the Collard Dove and Ruddy Shelduck was covered by a thick connective tissue capsule, formed of collagen fibers and some reticular fibers. Hepatocytes were arranged as irregular anastomosing cords of two cells. The cords were separated from each other's by blood sinusoids. The hepatocytes were large polygonal cells with large rounded nuclei. The portal triads containing branches of hepatic artery, portal vein, bile duct and lymph vessels.

Keywords


Article
SYNTHESIS AND CHARACTERIZATION OF NEW AZO DYE (1-(4-SULFONYL PHENYL AZO)-2-(7-CHLORO-4-[{4-(DIETHYL AMINO)-1-METHYL BUTYL}AMINO]QUINDINE FROM CHLOROQUINE DIPHOSPHATE AND STUDY ANTIBACTERIAL ACTIVITY
تحضير وتشخيص صبغة ازوية جديدة (1-(4- ازو سلفونات البنزيل)-2-(7- كلورو-4-[{-4( ثنائي اثيل امين)-1- بيوتيل المثيل} امين] كوينيدين من كلوروكوين ثنائي الفوسفيت ودراسة فعاليتها الضد بكتيرية.

Authors: Layla Addnan
Pages: 271-277
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New azo dye was synthesized by reaction dizonium salt of sulfanilic acid with antimelaria drug (chloro quin diphosphate).This product was characterized by FTIR(Fourier Transform Infrared)andUV.Visible spectrophotometer .The antibacterial activities of the compound was studied and evaluated using gram positive and negative gram stain. The purity of the dye was checked by thin layer chromatography(TLC) using solvent system( sec.Butanol-water-acetic acid)(2:2:1).The melting point of the purified dye was measured in an open capillary tube. We have been concluded that the prepared azo dye showed antibacterial activity against this micro organism.

Keywords

sulfanilic acid --- FTIR --- Azo dye


Article
EFFECTS OF ( Artemisia herba-alba) AND (Urtica dioica) ON SOME BLOOD PARAMETERS IN BROILER CHICK WHICH INFECTED EXPERIMENTALLY WITH Eimeria tenell
تأثير عشبتي الشيح والقريص على بعض المعايير الدمويه في فروج دجاج اللحم المخمج بطفيلي الايمريا تنيلا

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This study was carried out to determine the effect of the herb and Urtica dioica of the vitality of the Eimeria tenella, it conducted on 150 Ross broiler chicks, divided randomly on the seventh days into six groups. The last two groups (five and six) were considered as a positive and negative control groups, the first five groups were experimentally infected by 1000 oocysts/bird of Eimeria tenella at 17 days old directly in to the crop. The first four groups were given Artemisia herba alba and Urtica dioica According to concentrations for each group. Results were indicated a higher significant differences ( P<0.05 ) in the means of PCV for the groups that treated water herbs extracts of Artemisia herba alba and Urtica dioica (38.5±1.3 ,39.5±1.6%) respectively compared to positive and negative control groups (32.0 ±1.0, 33.0±1.13%) ; also , increase in the means of Hb concentration in the groups which treated with the Urtica dioica in food (14.2 ± 1.1 gm/100ml) and water extract (15.4 ± 1.2 gm /100 ml ) respectively compared to the control groups. A higher significant differences (P<0.05) were recorded in means of WBCs of the water extract of both herbs Artemisia herba alba and Urtica dioica (30.8 ± 0.3 ,31.0 ± 0.3 x103cell/ml) respectively compared to control group. While the differential count of WBCs were showed a significant decrease (P<0.05) in the heterophils of both herbs of water extracts of Artemisia herba alba and Urtica dioica (20.4±1.5% , 19.0±1.7%) respectively, especially at 5th week compared to control positive and negative groups. In conclusion used herb Urtica dioica especially aqueous extract enhance immune efficiency as well as the aqueous extract of the Artemisia herba-alba herb. And use the standard doses of 1% or less from Artemisia and Urtica dioica fodder and water are safety doses without mortality or side effects in broiler. Further studies are needed to (re) evaluate effect of Urtica dioica, Artemisia on other parasites.


Article
HISTOCHEMICAL STUDY OF MUCOPOLYSSACHARIDES IN STOMACH OF BUFFALO(BUBALUS-BUBALIS)
دراسة كيمياء نسجية للسكريات المتعددة المخاطية في المعدة للجاموس المحلي

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Ten samples of baffula stomach including rumen, reticulum, omasum Abomasum were collected from slaughter of Basrah .Tissue sections were done and stained with alcian blue and acridin orange dyes then photographed by light and fluorescent microscope .The histochemical study showed that all four parts of stomach consist of mucosa,submucosa ,muscular and serosal layer .The histochemical study showed that mucopolysacchrides distributed in all histological layers but with high density in the mucosal layer.


Article
STUDY THE EFFECTS OF TREATING EXPERIMENTAL VAGINAL CANDIDIASIS WITH THYME , OREGANO OIL AND NYSTATIN ON PITUITARY-GONADAL AXIS IN FEMALE RABBITS
دراسة أثار علاج الإصابة التجريبية بالمبيضات المهبلية بزيت الزعتر والمردقوش والنستاتين على محور الغدة النخامية والقند في إناث الأرانب

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Essentiaol oil of thymus vulgaris and origanum vulgare were extracted by hexane and analyzed to the component by GC-MAS Gas chromatography. in vivo study was done on seventy two healthy mature non pregnant female rabbits. Animals were divided into nine groups of eight animals for each one. the first group was injected by 0.1ml physiological saline vaginally and served as a negative control group. All other eight groups were immune suppressed by drenching each animal with (5 mg/kg) dexamethasone once daily for 7 days ,group 2 considered as a dexamethasone control group. Other seven groups were infected vaginally with Candida albicans by (1.5)×〖10〗^8 candida cells/ml once daily for 7 days group 3 considered as vaginal candida (positive) control. The sixth groups then treated once daily for 14 days with ( 0.1) ml of oils according to each group:- group 4 was treated with thyme oil orally, group5 treated with thyme oil vaginally ,group 6 treated with oregano oil orally, group 7 treated with oregano oil vaginally, group 8 treated by oral nystatin, Group 9 treated vaginally with nystatin. Blood was drawn from animals at the end of the experiment all experimental animals were sacrificed and pituitaries; ovaries and uteri were collected and fixed in 10% formalin for histological technique. result explained that candidiasis caused significant increase in progesterone, LH and FSH also decreased in estrogen compared to control. Treatment with thyme or oregano oils by two routs showed decrease in progesterone and an increase in estrogen than candidiasis group. So FSH and LH decreased in groups treated with two oils compared to candidisasis group but not reached to normal level. Nystatin decreased all reproductive hormones. results showed improvement of organs structures in treated with thyme or oregano oils


Article
COMPARATIVE HISTOLOGICAL AND HISTOCHEMICAL STUDY OF THE PAROTID GLAND IN BUFFALOES AND COWS
دراسة نسجية وكيمونسجية مقارنة للغدة النكفية في الأبقار والجاموس

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The present work designed to describe the histological and histochemical features of parotid gland in buffaloes and cows.The glands were collected from ten heads of buffaloes and cows used. The histological and histochemical study revealed that the parotid gland in buffaloes and cows are compound tubuloacinar-type, having dense connective tissue capsule, the parenchyma consists of acini, intercalated, striated and excretory duct, acini surrounded by myoepithelial cells.The parotidis a purely serous gland. histochemical study,demonstrated of mucopolysaccharide by using combined AB-PAS, acini shows weak to moderate reaction in buffaloes but weak reaction in cows.

Keywords

Parotid gland --- Buffalo --- Cow.


Article
COMPARATIVE HISTOLOGICAL STUDY OF GILLS OF TWO SPECIES IN FRESH WATER )CYPRINUS CARPIO) AND SEA WATER FISH (OTOLITHES RUBER) OF IRAQ
دراسة نسجية مقارنة للغلاصم في سمكة المياه العذبة الكارب وسمكة المياه البحرية النويبي في العراق

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In present study investigated of the histological structure of the gills of two fish species: Cyprinus carpio and Otolithes rubber which represented fresh water and seawater fish respectively. A total 20 specimens of freshwater fish (Cyprinus carpio) collected from Shatt-Al-Arab and 20 specimens of seawater fish(Otolithes ruber) were collected from Arabian Gulfs. The histological examination of gill samples showed that the seawater samples had a larger number of the chloride and pavement cells, compare with C. carpio. Also the gill raker of the O. rubber was appeared a cartilage and osseous tissue in the external side, while fibrous connective tissue, skeletal muscle layer and large amounts of adipose tissue was noticed in internal side, while the rake gills of the freshwater fish C. carpio was showed lake of the bone and skeletal muscle as well as adipose tissue.


Article
INFLUENCE OF SEASON ,SEX AND AGE ON HAEMATOLOGICAL CHARACTERISTICS, BODY WEIGHT AND RECTAL TEMPERATURE IN BLACK LOCAL QUAIL.
تاثير الموسم والجنس والعمر على المعايير الدمويه ووزن الجسم ودرجة حرارة المستقيم في السمان المحلي الاسود

Authors: Mohammad H.K
Pages: 340-348
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Effect of age, sex and season were measured during and before sexual maturation of black local quail males and females .Meals erythrocytes count showed higher value compared with that of adult females 6 month .Haemoglobin and hematocrit of adult males were significantly higher than those of adult females. However total leukocyte count and Hetrophil/Lymphocytes(H/L) ratio of the adult female were higher than those of males. Body weight of both sexes increased with age. In the present study the result indicated that erythrocyte, Hemoglobin concentrations and PCV values affected by season. As well as many of the Haematological parameters differed significantly (P<0.05) in accordance to the age, sex and season of local black feather.

Keywords

Quail --- Haemoglobin --- Lymphocytes


Article
SEROLOGICAL DETECTION OF ROTAVIRUS INFECTION IN BOVINE AND HUMAN
الكشف المصلي والجزيئي لاصابات الروتافايروس في الابقار والانسان

Authors: Enas R. Mohsin , Khalid H. Al-Jaff
Pages: 349-359
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Rotaviruses, causing acute gastroenteritis, that infect humans and animals around the world. There are many assays had been developed for the detection of rotavirus or the viral antigens. The present study was done on 79 samples of stool collected from pediatric patients with acute watery diarrhea aged from one months to 5 years admitted to Basrah Maternity and children hospital in Basrah province, during the period from October 2014 to February 2015. Ninety diarrheic fecal bovine samples were included in this study. All samples were used for the investigation and detection of rotavirus antigen by Enzyme-Linked Immunosorbent assay (ELISA).According to ELISA results, 10 out of 79(12.7%) pediatric stool samples rotavirus antigens were detected in children. Percentage (20.7%) of positive rotavirus antigen were detected in the patients at second age group (>6 months). Followed by 8% of patients at first age group (<6 months) these differences were not significant (P>0.05). The percentage of rotavirus antigen was higher in males patients (16.7%) compared to females (P>0.05) and also the differences were not significant differences (P>0.05). These results of rotavirus antigen detection in 90 diarrheic bovine fecal samples showed that this antigen was excreted by 56.7%of diarrheic calves. Additionally the higher non-significant (P>0.05) excretion percentage according to age was observed in 63.4 % of calves > 1 year old and the lower percentage(51.1%) was observed in the first age group( < 1year) calves old. The differences in sex were not significant (P>0.05) in the percentage of rotavirus antigen detection were also detected as 63.5% of male fecal samples show positive rotavirus antigen excretion whereas only 47.4% of female fecal samples were positive.

Table of content: volume:15 issue:1