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Article
HLA Profile in Iraqi Rheumatic Valvulitis Patients

Author: Ahmed A.A. Al-Hassan*MSc د. احمد الحسين
Journal: Journal of the Faculty of Medicine مجلة كلية الطب ISSN: PISSN: 00419419 / EISSN: 24108057 Year: 2007 Volume: 49 Issue: 2 Pages: 238-244
Publisher: Baghdad University جامعة بغداد

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Abstract

Summary: Background: Human leukocyte antigen (HLA) is the most polymorphic genetic system in man. The genes of this region influence susceptibility to certain disease.Objectives: This study was established to shed light on the possible association of HLA class I and II antigens with RV patients.Patients and Methods: Lymphocytotoxicity assay for HLA for class I and II typing had been done for (100) Iraqi patients suffering from rheumatic valvulitis (RV), the control groups consisting of (75 healthy individuals and 35 non rheumatic heart disease (NRHD) patients ). Results: The results showed a significant association of A33-Ags with these patients as compared with healthy and cardiac controls (P=0.005), (P=0.033) respectively. Another interesting finding was the low frequency of A1 in RV patients when compared with healthy control (p=0.002), suggesting that A1 allele may confer protective effect against this disease. In addition significant association between blood group B and RV was evident (p=0.04). An interesting observation was a strong association of blood group B and A33 among those patients (P<0.001).Conclusion: The present results are consistent with hypothesis that susceptibility to RV is genetically linked and in turn may be associated mainly with A33 in Iraqi patients. Key words: HLA, rheumatic valvulitis

Keywords

HLA --- rheumatic valvulitis


Article
Genetic characteristics and β-cell Autoimmunity in T1DM Children

Author: Eman M. Saleh
Journal: Journal of the Faculty of Medicine مجلة كلية الطب ISSN: PISSN: 00419419 / EISSN: 24108057 Year: 2007 Volume: 49 Issue: 4 Pages: 414-424
Publisher: Baghdad University جامعة بغداد

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Abstract

Background: TIDM is known to be polygenic disease that appears from the interaction of mutation in multiple genes including HLA. The autoimmune mediated destruction of pancreatic β-cells is reflected by the presence of autoantibodies against prominent antigens in the pancreatic β-cells. Objective: This study was designed to investigate the role of HLA-class I and class II antigens in the etiology of type 1 diabetes mellitus (T1DM) and also assessment of glutamic acid decarboxylase (GAD65) autoantibodies in the patients at the onset of the disease. Patients & Methods: Sixty T1DM patients who were newly onset of the disease (diagnosed less than five months) were selected. Eighty apparently healthy control subjects, matched with age, sex and ethnic backgrounds underwent the HLA-typing by lymphocytotoxicity assay. Finally 50 healthy individuals were selected randomly to undergo serological assessment of GAD65 autoantibodies using IRMA method. Results & Conclusion: At HLA-class I region, T1DM patients showed a significant increased frequency of antigen A9 (40.0 vs.18.75%) and B8 (28.33 vs.8.75%) as compared to control subject. At HLA-class II region, DR3 and DR4 were significantly increased in patients (53.33 vs.26.25% and 50.0 vs. 12.5% respectively) as compared to controls. In addition to that, T1DM was significantly associated with DQ2 (33.33 vs.15%) and DQ3 (40.0 vs. 20%) antigens as compared to controls, suggesting that these haplotypes had a role in disease susceptibility, while the frequency of DR2 and DQ1 antigens were significantly lowered in patients compared to controls (6.66 vs. 25% and 6.66 vs. 22.5% respectively). These molecules might had protective effect. Anti-GAD65 autoantibodies were present in 50% of T1DM children especially in older ages and in females more than males. High proportion of GADA was found in the patients carrying HLA-DR3/DR4 heterozygous. In conclusion, susceptibility to T1DM is genetically controlled.


Article
Association of HLA (Class I & II) and Susceptibility to

Author: Ikbal Kh. Al-Joofy Ph.D* د. اقبال الجوفي
Journal: Journal of the Faculty of Medicine مجلة كلية الطب ISSN: PISSN: 00419419 / EISSN: 24108057 Year: 2007 Volume: 49 Issue: 2 Pages: 265-266
Publisher: Baghdad University جامعة بغداد

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Abstract

Summary:Background: Hydatid cyst disease is a parasitic zoonotic disease caused by genus Echinococcus. This disease believed to has genetic background in it's aetiopathogenesis course . The aim of this study is to shed light on the possible correlation between HLA-class I (A,B,C) & HLA-class II (DR & DQ) antigens and the susceptibility to this disease. Patients & Methods: Fifty patient with hydatid cyst disease before undergoing surgical operation were investigated for HLA. Class I and class II by using microlymphocytotoxicity test. The results were compared with 115 healthy control. Results: Significant increased trend of HLA-A28 and A-11, -B18 and B-35, -DR3 and DR-11 (P<0.001, P<0.01) in patients with this disease as compared with healthy control. On the other hand, increased trend of HLA-28 (P<0.01) in patients with hydatid disease especially in those with a cyst location in liver. Conclusions: High frequency of HLA-A11 and –A28, B18 and –B35, -DR3 and –DR11 antigens may play major role in susceptibility to hydatid disease. HLA-A28 could be the most related antigen to this disease and acting as genetic marker that could in one way or another play crucial role in susceptibility especially in cases of hydatid disease in liver. Keywords: Hydatid cyst disease, HLA-typing


Article
Association between HLA-Class II Alleles and T-Cell Proliferation in Response to Enterovirus and Adenovirus

Author: Eman M. Saleh
Journal: Iraqi Academic Scientific Journal المجلة العراقية للاختصاصات الطبية ISSN: 16088360 Year: 2007 Volume: 6 Issue: 2 Pages: 102-108
Publisher: The Iraqi Borad for Medical Specialization المجلس العراقي للاختصاصات الطبية

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Abstract

BACK GROUND:Viruses may be involved in the pathogenesis of Type 1 Diabetes Mellitus (T1DM), either through direct β-cell infection or as triggers of autoimmunity.OBJECTIVE:To evaluate the T- cell proliferation in response to Enterovirus antigens including Coxsackievirus B and Poliovirus in addition to Adenovirus in an HLA- matched population of children with T1DM and children who were healthy.METHODS:A total of 60 Iraqi T1DM children were included in the presents study. They were new onset of the disease. For the purpose of comparisons, 50 apparently healthy control subjects were selected. HLA typing was measured by microlymphocytotoxicity, while methylthiazoltetrazolium (MTT) assay was used for lymphocyte proliferation by culturing peripheral blood lymphocytes (PBL) with Coxsackievirus B5, Adenovirus 3, 4, and 7, and Poliovaccin.RESULTS & CONCLUSION:No significant differences were shown in the PBL proliferative percentage in response to Con-A mitogen and tested viruses (CVB5 and Adenovirus) between T1DM and healthy controls, but PBL proliferative percentage of patients showed a significant decline in response to Poliovaccine. HLA class II (-DR3, DR4, DQ2 and DQ3) antigens were significantly increased in T1DM patients and they played an important role in the etiology of the disease. Strong T-cell proliferation in response to the tested viral antigens were observed to be related to HLA-DR4 and HLA-DQ3 antigens, whereas the HLA-DR3 and HLA-DQ2 alleles were associated with week responsiveness to the same antigens. However, in children with new- onset diabetes, responses were decreased and this could be caused by trapping of virus- specific T- cells in the pancreas


Article
The possible Association of HLA Class II with Bladder Cancer in Iraqi Patients

Author: Shatha M.J.Al-Khateeb
Journal: Journal of the Faculty of Medicine مجلة كلية الطب ISSN: PISSN: 00419419 / EISSN: 24108057 Year: 2007 Volume: 49 Issue: 4 Pages: 434-437
Publisher: Baghdad University جامعة بغداد

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Abstract

Background: - Genetic Factors have a major role in the development of bladder cancer. Objectives: - This study was carried out to shed a light on the possible association of HLA class II antigens and BC patients and to correlate this finding with the family history. Patients and Methodes :- Lymphocytotxicity assay had been used to assess HLA- typing of 65 BC patients and 50 healthy controls. Results:- comparison between BC patients and healthy controls showed several antigens deviations in their frequencies. HLA-DR1, HLA-DQ1 and HLA-DQ3 antigens were observed with increased frequencies in patients group with significant differences (P=0.000, 0.000 and 0.017 respectively). Moreover there was decrease frequency of HLA-DR7 in patients group (P=0.010). Stastical analysis showed non significant correlation of the specific HLA –Ags with family history. Conclusions: - This finding demonstrated that HLA-DR1, DR7, DQ1 and DQ3 might play a role in BC susceptibility. • Department of clinical biochemistry. Collage of Medicine Al-Mustansyriah University • Department of Microbiology. Collage of Medicine. Baghdad University • Ministry of Health.


Article
Possible Role of Interleukin-6 in Rheumatoid Arthritis

Author: Batool A. Al-Haidary *Ph.D د. بتول الحيدري
Journal: Journal of the Faculty of Medicine مجلة كلية الطب ISSN: PISSN: 00419419 / EISSN: 24108057 Year: 2007 Volume: 49 Issue: 2 Pages: 249-252
Publisher: Baghdad University جامعة بغداد

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Abstract

Summary:Background: Rheumatoid Arthritis (RA) is an autoimmune disease. Many etiological agents are proposed to play a role in its pathogenecity. One of these factors is cytokines such as Interleukin¬6. Material & Methods: ELISA method has been used for IL-6 estimation in 75 RA patients in comparison with 61 SLE as patient controls and 39 apparently healthy controls. Results: This study showed that there was an elevation of IL-6 in the sera of RA patients with high significant differences between RA patients and controls (P< 0.001). Moreover a good correlation between IL-6 level & RF titer were observed. However, for most patients with high IL-6 were shown to be HLA-DR4. Conclusions: Interleukin-6 play a crucial role in the disease which may be participate in the severity of RA & subsequently its treatment.Key words: RA, IL-6, RF, HLA-DR4, ELISA, Microlymphocytotoxicity

Keywords

RA --- IL-6 --- RF --- HLA-DR4 --- ELISA --- Microlymphocytotoxicity


Article
Possible association of HLA-DR and DQ Molecules with colorectal cancer in Iraqi patients

Authors: batool H. Al-Ghurabi --- ahmed sameer د. احمد سمير عبد الحميد
Journal: Journal of the Faculty of Medicine مجلة كلية الطب ISSN: PISSN: 00419419 / EISSN: 24108057 Year: 2007 Volume: 49 Issue: 3 Pages: 334-337
Publisher: Baghdad University جامعة بغداد

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Abstract


Article
HLA-CLASS II RISK ALLELES CONTROL T-LYMPHOCYTE PROLIFERATION IN RESPONSE TO ENTEROVIRUS AND ADENOVIRUS ANTIGENS AND IgG ANTIBODY PREVALENCE IN NEWLY DIAGNOSED T1DM CHILDREN
دور اليلات الخطورة لنظام مستضدات خلايا الدم البيض البشرية- الصنف الثانى فى استجابة الخلايا اللمفية ضد مستضدات الحمات المعوية والغدية وتفشى اضداد IgG هذه المستضدات في الأطفال المشخصين حديثاًبمرض السكر من النوع الأول

Authors: Nidhal abdul Mohymen نضال عبد المهيمن --- Eman M. Saleh إيمان مهدي صالح
Journal: IRAQI JOURNAL OF MEDICAL SCIENCES المجلة العراقية للعلوم الطبية ISSN: P16816579,E22244719 Year: 2007 Volume: 5 Issue: 3 Pages: 47-56
Publisher: Al-Nahrain University جامعة النهرين

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Abstract

Background: Viral infections are implicated in the pathogenesis of type 1 diabetes mellitus (T1DM) in a number of studies, and playing a role in the initiation of beta-cell damaging process.Objective: To evaluate the T- cell proliferation in response to enterovirus antigens including coxsackievirus B and poliovirus in addition to adenovirus in an HLA- matched population of children with T1DM and children who were healthy, in addition to screening for specific anti-viral IgG antibodies.Subjects and methods: A total of 60 Iraqi T1DM children were included in the presents study. They were newly diagnosed diabetics. For the purpose of comparisons, 50 apparently healthy children were selected. HLA typing was measured by microlymphocytotoxicity, while MTT assay was used for lymphocyte proliferation by culturing peripheral blood lymphocytes (PBLs) with Con-A, Coxsackievirus B5 (CVB5), Adenovirus 3, 4, and 7 serotypes, and Poliovaccine. Serum IgG against these viruses were detected quantitatively with an indirect ELISA.Results & conclusion: No significant differences were shown in the PBL proliferative percentage in response to Con-A mitogen and tested viruses (CVB5 and adenovirus) between T1DM and healthy controls, but PBL proliferative percentage of patients showed a significant decline in response to poliovaccine. Strong T-cell proliferation in response to the tested viral antigens were observed and was related to HLA-DR4 and HLA-DQ3 antigens, whereas the HLA-DR3 and HLA-DQ2 alleles were associated with week responsiveness to the same antigens. High significant mean proliferative percentage for all tested viruses were detected in those patients who were sero-positive IgG as compared to the sero-negative IgG diabetic children.Conclusion: In children with new- onset diabetes, responses were generally decreased, but higher in children who carried risk HLA- class II alleles and who were sero positive to anti- viral IgG antibodies.Key Words: T1DM, HLA class II alleles, Lymphocyte proliferation, Anti- CVB5 IgG, Anti- polio IgG, Anti-adeno IgG.

خلفية الدراسة: أثبتت العديد من الدراسات أن للفايروسات دورا تؤديه في إحداث أمراضية مرض السكر من النوع الأول وقد يكون لها دوراً في بدء عملية تحطيم خلايا بيتا في البنكرياس.هدف الدراسة: الهدف من الدراسة هو لتقييم الفعالية الوظيفية للخلايا اللمفية بعد تحفيزها بمستضدات الحمات المعوية والتي تشمل فايروس الكوكساكي نوعB- (CVB) وفايروس شلل الأطفال فضلاً عن ألحمة الغدية (Adenovirus) في مجموعة من الأطفال المصابين بمرض السكري من النوع الأول ومجموع من الأطفال الأصحاء المطابقين لمستضدات التطابق النسيجي (HLA) من الصنف الثاني فضلاً عن التقصي عن وجود الكلوبيولينات المناعية نوعIgG ضد هذه الفايروسات.طريقة العمل: شملت الدراسة ستون مريضاً حديثي الإصابة بمرض السكري النوع الأول (مشخصين بالإصابة خلال فترة أقل من خمسة أشهر).والذين تم اختيارهم من المركز الوطني للسكري / الجامعة المستنصرية للفترة من مايس 2004 ولغاية تشرين الأول 2005. ولغرض المقارنة تم اختيار مجموعة من الأطفال (50) طفلاً الاصحاء ظاهريا لغرض التحري عن وجود اليلات الخطورة لمستضدات التطابق النسيجي – الصنف الثاني ثم قياس الفعالية الوظيفية للخلايا اللمفية بعد حضنها مع الفايروسات المذكورة سابقاً باستخدام طريقة MTT فضلاً عن قياس المستويات المصلية للكلوبيولين المناعي IgG ضد الفايروسات الثلاثة باستخدام طريقة ELISA الغير مباشرة .النتائج: أظهرت الدراسة الحالية انخفاضاً غير معنوياً في الفعالية الوظيفية للخلايا اللمفية كاستجابة للمشطر Con-A وكذلك باستخدام فايروس CVB النوع المصلي (5) وفايروس الـ Adenoللانواع المصلية 3 ، 4 و 7 في الأطفال المرضى مقارنة بالأصحاء. ولكن هذا الانخفاض كان معنوياً فقط عند استخدام فايروس شلل الأطفال (P < 0.05). وكانت الزيادة في الفعالية الوظيفية للخلايا اللمفاوية معنوياً (P < 0.05) عند المرضى الحاملين للاليلات الخطورة من الصنف الثاني HLA-DR3 ; - DR4 ; - DQ2 ; - DQ3 مقارنة بالمرضى الحاملين للاليلات الأخرى وكذلك عند المرضى الموجبين للكلوبيولينات المناعية نوع IgG مقارنة بالمرض السالبين .الاستنتاج: انخفضت الاستجابة المناعية عموماً في الأطفال المصابين ولكنها ازدادت في الأطفال الحاملين للاليلات الخطورة HLA من الصنف الثاني و الموجبين للكلوبيولينات المناعية نوع IgG المضادة للفايروسات.مفاتيح الكلمات: مرض السكر من النوع الأول ، مستضدات التطابق النسيجي - الصنف الثاني ، الفعالية الوظيفية للخلايا اللمفية ، الكلوبيولينات المناعية نوع IgG ضد فايروس الكوكساكي B النوع المصلي 5 وشلل الأطفال والحمى الغدية.

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