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CTX-M producing carbapenem- resistant Klebsiella pneumoniae from children with upper respiratory tract infections in Hilla city

Author: Fatima Moeen Abbas
Journal: Mesopotamia Environmental Journal بيئة وادي الرافدين ISSN: 24102598 Year: 2017 Issue: Special Issue C Pages: 104-110
Publisher: Babylon University جامعة بابل

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Abstract

This study was attempted to find out the prevalence of blaCTX-M gene among carbapenem resistant Klebsiella pneumoniae from upper respiratory tract infections . In total ,100 throat samples were collected from children attending Babylon Teaching Hospital for Maternity and Pediatric at a period from November, 2015 to the end of February, 2016 .Fourteenth (14%) isolates were specified as K.pneumoniae, these were screened phenotypically for extended spectrum β-lactamase (ESBL) production by disk combination method. Nine (64.3%) isolates were found to be screen-positive. ESBL-producing isolates were submitted to antimicrobial susceptibility testing using Kirby-Bauer disk diffusion method. The highest rates of resistance were observed for penicillin antibiotics (ampicillin and cloxacillin) with (88.88%) and (77.77%) resistance rate, respectively. The lowest rate for carbapenem antibiotics (imipenem, 11.11% and meropenem, 22.22%). Carbapenem resistant isolates were checked by Polymerase Chain Reaction (PCR) method for the presence of blaCTX-M , 2 (100%) isolates gave positive result.

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