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Article
The Association of Severe Toxoplasmosis and Some Cytokine Levels in Breast Cancer Patients

Authors: Entsar Jabbar Saheb --- Maha Mustafa Assim
Journal: Iraqi Journal of Science المجلة العراقية للعلوم ISSN: 00672904/23121637 Year: 2018 Volume: 59 Issue: 3A Pages: 1189-1194
Publisher: Baghdad University جامعة بغداد

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Abstract

Toxoplasma gondii is an opportunistic pathogen in which the reactivation of a latent infection can cause death in congenitally infected fetuses, newborns, and immunocompromised patients. This study aimed to determine the seropositivity of toxoplasmosis infection and the possible association with Interleukin-12 (IL-12) and Interleukin-23 (IL-23) cytokines in breast cancer patients. In this study, 190 women were enrolled. All serum samples were tested for T. gondii immunoglobulins (IgG and IgM) antibodies and (IL-12, IL-23) levels using ELISA technique. The result of this study showed that breast cancer patients recorded the highest percentage of toxoplasmosis infection. There were no positivity rates for anti- Toxoplasma IgM in breast cancer patients while the positivity percentage for anti- Toxoplasma IgM among the control group was (7.00%). Furthermore, the seroprevalence of anti- Toxoplasma IgG was the highest in the age group (31- 40) years in patients with breast cancer while the highest mean titer of the IL-12 is restricted to ages (21-30) years in the control groups who are seropositive to anti- Toxoplasma IgG. Although, in patients with breast cancer who are seropositive to anti- Toxoplasma IgG, the highest mean titer of the IL-23 was in ages (21-30) years. Since most immunosuppressive patients are exposed to various possible risk factors including Toxoplasma primary infection or reactivation, so it is important to diagnose and treat toxoplasmosis in breast cancer patients to reduce the consequences of the infection.


Article
The role of Some Tumour Associated Genes (CA9, WT1, PRAME) in diagnosis and prognosis of Breast Cancer.
دور بعض الجينات المرتبطة بالورم (CA9, WT1, PRAME)في التشخيص والتنبؤ المستقبلي لسرطان الثدي

Authors: Jawdat N. Gaaib جودت نوري غائب --- Maisaa G. Jumaa and ميساء غازي جمعة
Journal: Albahir journal مجلة الباهر ISSN: 23125721 Year: 2018 Volume: 7 Issue: 13-14 Pages: 29-40
Publisher: AL-Abbas Holy Shrine العتبة العباسية المقدسة

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Abstract

The aim of the present study is to assess the possible diagnostic and prognostic significance of certain tumour associated genes (CA9, WT1, and PRAME) in relation to tumour size and lymph node status. In order, the expression of these factors were measuredin the peripheral blood of breast cancer patients (N=55), patients with benign breast lesions (N=10) and apparently healthy controls (N=20). Quantitative real time polymerase chain reaction (qRT-PCR) was used to assess the expression of the target biomarkers. In the breast cancer samples 50(91%) samples were CA9-positive,8(14.54%) wereWT1-positive and 5(9.09%) were PRAME-positive samples. The expression of CA9-positive was significantly higher in breast cancer sample compared to benign tumour samples and healthy controls. For lymph node status, the results of all three genes showed that the highest percentage of positive samples 25(50%), 5(60%) and 4(80%) for CA9, WT1 and PRAME genes respectively, were multiple for lymph node status. The tumour size was significantly associated with the increasedCA9 and WT1 genes expression with tumour size 2.0-2.9 cm, while for PRAME gene the highest percentage of positive samples were with tumour size 1.0-1.9 cm. This study showed that CA9 gene can be a useful tool for discrimination between malignant and non-malignant breast tumours, the results may also indicatethe diagnostic and prognostic values for this gene. However, further analysis of a bigger cohort are required to consolidate these initial findings.

اﳋﻼﺻﺔ ( واﻟﺘﻲ ﻗﺪ ﻳﱰدد اﻟﺘﻌﺒﲑ ﻋﻨﻬﺎ ﰲ أﻧﻮاع ﳐﺘﻠﻔﺔ ﻣﻦ TAASﻫﻨﺎك ﳎﻤﻮﻋﺔ ﻣﻦ اﳌﺴﺘﻀﺪات اﳌﺮﺗﺒﻄﺔ ﺑﺎﻟﻮرم ) اﻟﴪﻃﺎن. ﻗﺪ ﻳﻠﻌﺐ اﻟﺘﻌﺒﲑ ﻋﻦ ﻫﺬه اﳉﻴﻨﺎت دورا ﺣﺎﺳﲈ ﰲ اﻟﺘﺸﺨﻴﺺ و اﻟﺘﻨﺒﻮء اﳌﺒﻜﺮ ﺑﻤﺮض اﻟﴪﻃﺎن، ﻛﲈ اﳖﺎ ﺗﻌﺪ ﻣﻦ اﻫﻢ اﻻﻫﺪاف اﻟﺘﻲ ﻗﺪ ﻳﺴﺘﻬﺪﻓﻬﺎ اﻟﻌﻼج اﳌﻨﺎﻋﻲ. ﲥﺪف اﻟﺪراﺳﺔ اﳊﺎﻟﻴﺔ اﱃ ﲢﺪﻳﺪ ﻣﺴﺘﻮﻳﺎت اﻟﺘﻌﺒﲑ ﻟﺜﻼﺛﺔ ( ﰲ اﻟﺪم اﳌﺤﻴﻄﻲ اﳌﺄﺧﻮذ ﻣﻦ ﻣﺮﴇ ﴎﻃﺎن CA9, WT1 and PRAMEﻣﻦ ﻫﺬه اﳉﻴﻨﺎت اﳌﺮﺗﺒﻄﺔ ﺑﺎﻟﻮرم ) اﻟﺜﺪي ﺑﺎﳌﻘﺎرﻧﺔ ﻣﻊ ﻋﻴﻨﺎت دم ﳌﺮﴇ اورام اﻟﺜﺪي اﳊﻤﻴﺪة وﻷﺷﺨﺎص اﺻﺤﺎء ﻛﻤﺠﺎﻣﻴﻊ ﺳﻴﻄﺮة. ﺗﻢ ﺗﻘﺪﻳﺮ اﻟﻘﻴﻤﺔ اﻟﺘﺸﺨﻴﺼﻴﺔ ﳍﺬه اﳉﻴﻨﺎت ﻣﻦ ﺧﻼل ﻣﻘﺎرﻧﺔ ﻣﺴﺘﻮﻳﺎت اﻟﺘﻌﺒﲑ ﻣﻊ ﻛﻞ ﻣﻦ ﺣﺠﻢ اﻟﻮرم, و ﺣﺎﻟﺔ اﻟﻌﻘﺪ اﻟﻠﻴﻤﻔﺎوﻳﺔ. 10 ﻋﻴﻨﺔ ﻣﻦ اﳌﺘﱪﻋﲔ اﻷﺻﺤﺎء، و 20 ﻣﺮﻳﻀﺔ ﻣﺼﺎﺑﺔ ﺑﴪﻃﺎن اﻟﺜﺪي و55( ﻣﻦ PBﲨﻌﺖ ﻋﻴﻨﺎت اﻟﺪم اﳌﺤﻴﻄﻲ ) ﻧﺴﺎء ﻛﺎن ﺣﺪﻳﺜﺎ ﻗﺪ ﺗﻢ ﺗﺸﺨﻴﺺ اﺻﺎﺑﺘﻬﻢ ﺑﺎورام اﻟﺜﺪي اﳊﺪﻳﺜﺔ, اﺳﺘﺨﺪﻣﺖ اﳌﺠﻤﻮﻋﺘﲔ اﻻﺧﲑﺗﲔ ﻛﻤﺠﺎﻣﻴﻊ ﺳﻴﻄﺮة (. أﺷﺎرت ﻧﺘﺎﺋﺞ اﻟﺪراﺳﺔ إﱃ أن RT-PCRوﺗﻢ ﲢﻠﻴﻞ اﻟﻌﻴﻨﺎت ﺟﺰﻳﺌﻴﺎ ﺑﺎﺳﺘﺨﺪام ﺗﻘﻨﻴﺔ ﻋﻜﺲ ﺗﻔﺎﻋﻞ ﺳﻠﺴﺔ اﻟﺒﻠﻤﺮة ) ﻣﻦ اﻟﻌﻴﻨﺎت ﻣﻮﺟﺒﺔ ﳉﲔCA9، 8 (14.54٪( ﻋﻴﻨﺔ اﳚﺎﺑﻴﺔ ﳉﲔ)91%) 50 ﻋﻴﻨﺔﻟﴪﻃﺎن اﻟﺜﺪي، ﻛﺎﻧﺖ 55ﻣﻦ ﺑﲔ ﰲ ﻋﻴﻨﺎت اﻟﴪﻃﺎن أﻋﲆ ﺑﻜﺜﲑ CA9. ﻛﺎن ﺗﻌﺒﲑ ﺟﲔPRAME( ﻣﻦ اﻟﻌﻴﻨﺎت ﻣﻮﺟﺒﺔ ﳉﲔ 9.09%) 5 و WT1 ﺑﺎﳌﻘﺎرﻧﺔ ﻣﻊ ﻋﻴﻨﺎت اﻷورام اﳊﻤﻴﺪة واﻻﺻﺤﺎء، ﰲ ﺣﲔ أن ﻛﻞ ﻣﻦ اﳉﻴﻨﲔ اﻷﺧﺮﻳﻨﻠﻢ ﻳﻈﻬﺮا ﺗﻌﺒﲑ ﰲ ﻋﻴﻨﺎت اﻻورام اﳊﻤﻴﺪة وﻋﻴﻨﺎت اﻻﺻﺤﺎء. ﻋﻨﺪ ﻣﻘﺎرﻧﺔ اﻟﺘﻌﺒﲑ اﳉﻴﻨﻴﻠﻠﺠﻴﻨﺎت اﻟﺜﻼﺛﺔ ﺑﺎﻟﻨﺴﺒﺔ إﱃ وﺿﻊ اﻟﻌﻘﺪة اﻟﻠﻴﻤﻔﺎوﻳﺔ, أﻇﻬﺮت CA9، WT1( ﻟﻠﺠﻴﻨﺎت 80%) 4( و 60%) 5( و 50%) 25ﻧﺘﺎﺋﺞ اﳉﻴﻨﺎت اﻟﺜﻼﺛﺔ أن أﻋﲆ ﻧﺴﺒﺔ اﻟﻌﻴﻨﺎت اﻹﳚﺎﺑﻴﺔ ﻋﲆ اﻟﺘﻮاﱄ، ﻛﺎﻧﺖ ﰲ ﺣﺎﻟﺔ اﻟﻌﻘﺪة اﻟﻠﻴﻤﻔﺎوﻳﺔ اﳌﺘﻌﺪدة. وﻓﻘﺎ ﳊﺠﻢ اﻟﻮرم أﻇﻬﺮت اﻟﻨﺘﺎﺋﺞ أن ﻫﻨﺎﻟﻚ PRAMEو ، ﰲ ﺣﲔ أن 2،9-2،0 ﻣﻊ اﻻورام ذو اﳊﺠﻢ WT1 وCA9ﻋﻼﻗﺔ ذات دﻻﻟﺔ إﺣﺼﺎﺋﻴﺔ ﺑﲔ زﻳﺎدة اﻟﺘﻌﺒﲑ ﻟﻠﺠﻴﻨﺎت . ﻛﺄﺳﺘﻨﺎﺗﺞ أﺛﺒﺘﺖ اﻟﺪراﺳﺔ اﳊﺎﻟﻴﺔ أن 1،9-1،0 ﻛﺎﻧﺖ أﻋﲆ ﻧﺴﺒﺔ اﻟﻌﻴﻨﺎت اﻹﳚﺎﺑﻴﺔ ﻣﻊ ﺣﺠﻢ اﻟﻮرم PRAMEﺟﲔ ﻳﻤﻜﻦ أن ﻳﻜﻮﻧﺬو ﻗﻴﻤﺔ ﺗﻔﺮﻳﻘﻴﺔ ﻟﻠﺘﻤﻴﺰ ﺑﲔ أورام اﻟﺜﺪي اﳋﺒﻴﺜﺔ ﻣﻦ ﺗﻠﻚ ﻏﲑ اﳋﺒﻴﺜﺔ، ﻛﺬﻟﻚ ﺗﺸﲑ اﻟﻨﺘﺎﺋﺞ CA9ﺟﲔ اﱃ اﳌﺰﻳﺪ ﻣﻦ PRAME وWT1اﱃ اﻟﻘﻴﻤﺔ اﻟﺘﺸﺨﻴﺼﻴﺔ واﻟﺘﻨﺒﺆﻳﺔ ﳍﺬا اﳉﲔ. ﻣﻦ ﻧﺎﺣﻴﺔ أﺧﺮ￯ ﻗﺪ ﳛﺘﺎج اﳉﻴﻨﺎن اﻟﺪراﺳﺎت اﳉﺰﻳﺌﻴﺔ ﻟﻠﻜﺸﻒ ﻋﻦ دورﻫﻢ ﰲ اﻣﺮاﺿﻴﺔ ﴎﻃﺎن اﻟﺜﺪي.

Keywords

Breast cancer --- WT1 --- CA9 --- PRAME --- Breast cancer --- WT1 --- CA9 --- PRAME


Article
Left Ventricular Dysfunction in Breast Cancer Patients Receiving Trastuzumab: An Observational Study in a Cohort of Iraqi Breast Cancer Patients
أنخفاض وظيفة البطين الأيسر لدى مرضى سرطان الثدي المعالجين بعقار التراستوزوماب

Author: Musaab R. Al-Bayati الدكتور مصعب راجي البياتي
Journal: Thi-Qar Medical Journal مجلة ذي قار الطبية ISSN: 19929218 Year: 2018 Volume: 15 Issue: 1 Pages: 105-113
Publisher: Thi-Qar University جامعة ذي قار

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Abstract

AbstractBackground : Cardiotoxicity is a well recognized adverse event of trastuzumab. This study was designed to evaluate the frequency of decline in left ventricular ejection fraction (LVEF) to less than 50% in patients with breast cancer who have received trastuzumab in a daily practice setting. Methods : Retrospective observational study of 116 patients with HER2 positive breast cancer treated with trastuzumab in one center between May 2012 and January 2017. Results : Left ventricular ejection fraction decline to less than 50% was observed in 7 patients (6%). Patients above 70 years of age have the highest frequency of LVEF decline (33%). All events of LVEF decline was reported in the first six months of treatment. Prolonged administration of trastuzumab beyond one year was not associated with adverse cardiac events. Previous anthracycline exposure and chest irradiation was not found to be associated with LVEF decline. Conclusions : Events of LVEF decline to less than 50% associated with trastuzumab use seems to be slightly higher in general daily practice than in clinical trial setting, especially in patients above 70 years, calling for better patient selection. First year of trastuzumab therapy carries the highest risk of cardiac events which necessitates a close cardiac monitoring, thereafter, periodic LVEF measurements can be reduced to reduce the costs.


Article
Evaluation of Estridiol and Some Antioxidant in Breast Cancer Iraqi Women

Authors: Ghufran Saad Nsaif --- Amer Hassan Abdallah --- Najwa Shehab Ahmed --- Wafaa Raji Alfatlawi
Journal: Al-Nahrain Journal of Science مجلة النهرين للعلوم ISSN: (print)26635453,(online)26635461 Year: 2018 Volume: 21 Issue: 1 Pages: 35-40
Publisher: Al-Nahrain University جامعة النهرين

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Abstract

Cancer is the second reason of deaths worldwide while breast cancer is the more spread cancer in women, comprising 29 % from all cancers that they are affected Samples were obtained from patients undergoing surgery in center from breast cancer in oncology teaching hospital Al-Eluia hospital for woman care All blood samples were taken of the patients (110 women) and divided into four groups benign, malignant, radiotherapy and control group In order to measurement estrogen levels which are influenced by the menstrual cycle, the main groups were furthermore divided into sub-groups, premenopausal and postmenopausal women The mean value ± SE of Body Mass Index (BMI) for control, benign, malignant and radiotherapy breast cancer were showed a non-significant change in the comparison of groups according to the Duncan test Estrogen were increased before therapy but decreased following therapy The levels of malondialdehyde (MDA)were elevated in patients after radiotherapy The mean value ± SE of vitamin E were decreased in studied groups Present study aims to study the effects of serum estrogen, vitamin E and antioxidant status in breast cancer patients

Keywords

Estrogen --- Vitamin E --- MDA --- BMI --- Breast cancer


Article
12.THE LEVEL OF 27-HYDROXYCHOLESTEROL AND OXYSTEROL 7 α-HYDROXYLASE (CYP7B1) IN TISSUES OF WOMEN WITH BREAST TUMORS

Authors: Zahraa K. Mohammed زهراء كاظم محمد --- Hassan H. AL-Saeed حسن حسين العبد --- Anees K. Nile انيس خليل نايل
Journal: IRAQI JOURNAL OF MEDICAL SCIENCES المجلة العراقية للعلوم الطبية ISSN: P16816579,E22244719 Year: 2018 Volume: 16 Issue: 2 Pages: 201-206
Publisher: Al-Nahrain University جامعة النهرين

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Abstract

Background:In Iraq, breast cancer is the commonest type of malignancy in females. The disease is a genetically and clinically heterogeneous. The cholesterol metabolite 27-Hydroxycholesterol (27HC), a primary metabolite of cholesterol and an estrogen receptor (ER) and Liver X receptor (LXR) ligand, increases ER-positive breast cancer. 27-hydroxycholesterol (27HC) is metabolized by oxysterol 7α-hydroxylase (CYP7B1), CYP7B1 expression has been decreased in ER-positive tumors compared with normal breast tissues.Objective:To study and investigate the possible association of clinic-pathological parameters 27HC and CYP7B1 level in sera of women with benign and malignant breast tumors and in control group by ELISA technique and Investigate the possible relationships of 27HC with CYP7B1.Methods:This case control study was conducted on sixty patients with breast diseases were divided into three group, group I contained twenty patients with benign breast diseases, group II consisted of twenty premenopausal patients with breast cancer. Group III comprised twenty postmenopausal patients with breast cancer with the mean age and standard deviation (25.25±7.87, 38.65±6.28, 58.5±7.02 years). 27HC and CYP7B1 were measured in tissues by instrument ELISA technique.Results:The tissue homogenates of women with premenopausal and postmenopausal breast cancer groups showed a significant elevation of 27HC in comparison with benign (p> 0.001), whereas there is no significant difference observed between both breast cancer groups (p= 0.542). The tissue homogenates of women with premenopausal and postmenopausal breast cancer groups showed a significant decrease of CYP7B1 concentration in comparison with benign (p= 0.003) and (p= 0.001), whereas there is no significant difference observed between both breast cancer groups (p= 0.868).Conclusion:A higher incidence of 27HC and a lower incidence CYP7B1 were obtained in malignant than benign breast tumor tissues with positive estrogen receptors. These indicate that the levels of 27HC and CYP7B1 in breast tumor tissues may be used as new biochemical markers for breast tumor prognosis.Keywords:27-Hydoxycholesterol, CYP7B1, breast cancer, estrogen receptorCitation:Mohammed ZK, AL-Saeed HH, Nile AK. The level of 27-hydroxycholesterol and oxysterol 7α-hydroxylase (CYP7B1) in tissues of women with breast tumors. Iraqi JMS. 2018; 16(2): 201-206. doi: 10.22578/IJMS.16.2.12


Article
Evaluation of lipid profile among Iraqi patients with the Breast Cancer

Author: Mushtaq T. Hasan1 , Zafir H. Al-Qaissi 2 , Wathiq A. Al-Draghi 1
Journal: Iraqi Journal of Biotechnology المجلة العراقية للتقانات الحياتية ISSN: 18154794 Year: 2018 Volume: 17 Issue: 2 Pages: 68-74
Publisher: Baghdad University جامعة بغداد

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Abstract

Breast cancer is most common cancer among women worldwide. In this study, the association between serum concentration of cholesterol, triglycerides( TG|), low density lipoproteins (LDL), high density lipoproteins (HDL),( VLDL) very low density lipoproteins and breast cancer has been investigated. These case control study include three groups: thirty women with breast cancer and fifteen healthy women as control group. Serum lipids: cholesterol,( LDL),( HDL) and (VLDL) were analyzed in all subjects of study using conventional method. The results of this study showed that there was a significant increase in the rate of the levels of total cholesterol (TC), triglycerides (TG), low density lipoproteins( LDL),( VLDL) very low density lipoproteins in the work group when compared with control (P=0.028, P=0.017, P=0.024 and P=0.033) respectively, except high density lipoproteins (HDL). We conclude that high concentration of cholesterol, triglyceride, (LDL), and (VLDL) may be associated with breast cancer.

Keywords

: Lipid profile --- Breast cancer --- TG --- HDL --- VLDL .


Article
Prolactin Serum Levels and Breast Cancer: Relationships with Hematological Factors among Cases in Karbala Province, Iraq

Author: Jaafar Khalaf Ali, Salim Hussein Hassan, Mohammed A. Merzah
Journal: Medical Journal of Babylon مجلة بابل الطبية ISSN: 1812156X 23126760 Year: 2018 Volume: 15 Issue: 2 Pages: 178-181
Publisher: Babylon University جامعة بابل

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Abstract

Background: About one million of women are diagnosed with breast cancer globally and nearly half of whom will die from cancer. Breastcancer is the most common cancer and the leading of mortality among women. The present study aimed to find out the relationship breastcancer and levels of PRL and influence on some hematological parameters. Materials and Methods: A total of 71 specimens were collectedfrom females with breast cancer. Blood specimens were collected, and a blood group, packed cell volume (PCV), hemoglobin (Hb), erythrocytesedimentation rate (ESR), and prolactin (PRL) level was evaluated. Results: The results show that most breast cancer cases were agegroup 40–50 years and less common among other age groups. The married women were 97% and the unmarried was 3% only. Most studiedcases (43%) were O+ and (26%) were A+ blood group, in compare to other blood groups. In addition, many women show a slight decrease inHb and PCV level (<11.0 g/dl, <36% respectively), on the other hand, the mean value of ESR was increased nonsignificantly (P > 0.05). ThePRL levels were increased (31.5 ng/ml) in compare to the range of normal value (14.5 ng/ml) in women at all age groups. Conclusions: Thestudy concludes that there was a relationship between PRL level and breast cancer with a highly significant value.


Article
Ki‑67 Expression as an Indicator of Invasiveness in Patients with Breast Cancer

Author: Ahmed Salah Mahdi, Hayder Hussein Ibrahim1, Ayad Ahmad Mohammed
Journal: Medical Journal of Babylon مجلة بابل الطبية ISSN: 1812156X 23126760 Year: 2018 Volume: 15 Issue: 4 Pages: 271-275
Publisher: Babylon University جامعة بابل

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Abstract

Background: Tumor markers have a key role in guiding breast cancer management protocols and predicting prognosis. Objective: The aimof the study is to explore the role of Ki‑67 expression in breast cancer and correlate it to well‑known indicators of invasiveness such as age,tumor size, grade, hormonal receptors, and lymph nodes involvement. Materials and Methods: This retrospective study was conductedon (214) patients who were newly diagnosed with breast cancer and referred to Azadi Teaching Hospital/Duhok‑Iraq, from November 1st,2016, to October 31st, 2017. Data regarding patient’s demographics, tumor size, histological type and grade, nuclear grade, and lymph nodeinvolvement were obtained from medical records. Patients underwent either mastectomy with axillary lymph node dissection or breastconservation surgery. Collected specimens were sent for histopathological examination and immunohistochemistry assessments of estrogenreceptor (ER), progesterone receptor (PR), human epidermal growth factor 2 (HER2), and Ki67 expressions. Results: Our study showed thathistological grade, nuclear grade, mitotic index, and HER2 status were positively correlated to Ki67 index (P < 0.05). Furthermore, ER andPR status showed a negative correlation to Ki67 index (P < 0.05). Age, tumor size, and the number of lymph nodes involved displayed nosignificant correlation to Ki67 level (P = 0.080, 0.738, and 0.888), respectively. Conclusion: Results of this study confirmed that Ki67 indexare significantly correlated with tumor grade, ER, PR, and HER2 status. However, Ki67 expression association with other clinic‑pathologicalparameters such as age, tumor size, or lymph node involvement is not recognized and requires further studies.


Article
The Prevalence of Human Papilloma Virus among Breast Cancer Women in Relation with IL-8 Level in Kirkuk Province
الانتشار المصلي لفيروس الورم الحليمي البشري في النساء المصابات بسرطان الثدي وعلاقته مع IL-8 في مدينة كركوك

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Abstract

The study aimed at evaluating the frequency of HPV in breast cancer women in relation with serum IL-8 level. The study that carried out in Kirkuk city from 15th of January 2017 to 15th of June 2017, a total of 150 breast cancer women who admitted to Kirkuk oncology cancer and 96 healthy control women whose ages were between 30-75 years. Patients and control were investigated for detection of human papilloma virus (HPV) 16 E7 protein and interleukin-8 (IL-8) by ELISA technique. The rate of breast cancer women with HPV 16 E7 (30.67%) was higher than that in control women (3.125%) with highly significant relation. The highest rate of breast cancer women was within the age group 40-49 years with no significant relation between breast cancer and age. The study showed that the highest means of IL-8 level (36.92 pg/ml) were found in breast cancer women without HPV, 29.09 pg/ml in HPV positive and 6.45 pg/ml in the control group with highly significant relation. There was a highly significant relation of WBCs, lymphocytes and neutrophils count and Hb level among the study groups concerning HPV. WBCs count and hemoglobin level were higher in the control group than in breast cancer women. The mean absolute lymphocytes count was higher in breast cancer women with HPV infection than those without HPV and the control group (4.13, 1.80 and 1.89 respectively), while the least mean of neutrophils count was reported in breast cancer women with HPV infection. Conclusions: It was concluded that there was a highly significant relation between HPV and EBV infection with breast cancer.

تهدف الدراسة الى تقييم الانتشار المصلي لفيروس الورم الحليمي البشري في النساء المصابت بسرطان الثدي مع علاقته به مستوى IL-8. أجريت الدراسة الحالية في مدينة كركوك (مركز كركوك للأمراض السرطانية) في الفترة الممتدة من 15 كانون الثاني 2017ولغاية 15 حزيران 2017 وشملت الدراسة 150 امرأة مصابة بسرطان الثدي و96 امرأة غير مصابة كمجموعة سيطرة في الدراسة حيث تراوحتاعمار المجموعتين من 30 الى 75 سنة ، وتم اجراء فحوصات الـ ELISA للكشف عن فايروسالورم الحليمي البشري في المجموعتين فضلا عن الكشف عن مستوى IL-8.اظهرت النتائج ان نسبة إصابة مريضات سرطان الثدي بفيروس البشري الحليمي (30,67%) والتي كانت اعلى مما هو الحال في مجموعة السيطرة (3,125%). كانت أعلى نسبة للمريضات المصابات بسرطان الثدي ضمن الفترة العمرية 40-49 سنة ولم يكن هنالك أي تأثير مع سرطان الثدي. قد أظهرت الدراسة ان اعلى مستوى لـ IL-8 ( 36,92 بيكوغرام/مليلتر) وجد في مريضات سرطان الثدي مقارنة بالمريضات المصابات بفايروس الورم الحليمي البشري (29,09 ، 28.74 و18,74 بيكوغرام/مليلتر على التوالي) ومجموعة السيطرة (6,45) مع وجود علاقة إحصائية بهذا الصدد. وان هنالك علاقة إحصائية قوية بين تعداد خلايا الدم البيضاء والخلايا العدِلة والخلايا اللمفية ومستوى الهيموغلوبين في مجاميع البحث بما يخص الإصابات بفايروس الورم الحليمي. كان تعداد خلايا الدم البيضاء ومستوى الهيموغلوبين اعلى في مجموعة السيطرة مقارنة بمريضات سرطان الثدي بينما كان تعداد الخلايا اللمفية عاليا في مريضات سرطان الثدي اللواتي يحملن الإصابة بفايروس الورم الحليمي البشري مقارنة بغير المصابات ومجموعة السيطرة ( 4,13 ، 1,80 و1,89 على التوالي). كان اقل تعداداً للخلايا العدلة قد سُجّل في مريضات سرطان الثدي المصابات بفايروس الورم الحليمي البشري. من هذا يُستنتج ان هنالك علاقة قوية بين سرطان الثدي والاصابة بفايروس الورم الحليمي البشري ومستوى IL-8.


Article
A study of The Frequency and Type of Mutation in Exon 11 in BRCA1 and BRCA2 Genes In Breast Cancer Women With Positive Family History
دراسة التكرار و نوع الطفرة في اكسون11 في جين BRCA1 و BRCA2 في سرطان الثدي للنساء ذات التاريخ العائلي الايجابي

Authors: Noor Jassim M نور جاسم محمد --- Ammar Ahmed Sultan عمار احمد سلطان --- Fhadil Ahmed Dalaf فاضل احمد دلاف
Journal: Diyala Journal of Medicine مجلة ديالى الطبية ISSN: 97642219 Year: 2018 Volume: 14 Issue: 1 Pages: 106-114
Publisher: Diyala University جامعة ديالى

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Abstract

Background:Both BRCA1 and BRCA2 are tumor suppressor gene and are inherited as an autosomal dominant. The cumulative lifetime risk of developing invasive breast cancer for those with BRCA1 or BRCA2 mutations ranges from 53% to 89%. Familial breast cancer represent less than 10% of all cancers of the breast, and cancers related to BRCA1 and BRCA2 familial disease account only for three-fourths to two-thirds of these cases. In women younger than 35 year old with CA breast, 10% to 15% have a BRCA1 mutation. Females with mutations involving BRCA 1/2 who are already affected by breast cancer have an increased risk of breast cancer involving the other breast of 52% and 66%.Objective: To identify the frequency and the type of mutation in exon 11 in the genes BRCA1 and BRCA2 in breast cancer women with positive family history. Patients and Methods: This is a prospective study of fourteen females having breast cancer with positive family history of breast cancer. The study done in Baquba Teaching Hospital over a period of eight months (October 2016-July 2017). The age range of the patients was 40-70 years. Genomic DNA was extracted from lymphocytes yielded from the peripheral blood using the salting out procedure. Primers were used to amplify exon 11 region of the genes BRCA1 and BRCA2 by using polymerase chain reaction (PCR) cycling.Results: A total of eight variants in the BRCA1 gene and four variants in the BRCA2 gene were seen. Only one deleterious germline mutation in BRCA1 was detected in 1/14 (7.14%). The patient with deleterious mutation was 31 year-old and was having strong family history of the disease (two relatives, first and second degree). The sequence variant of the mutation was c.795_789delTT with an effect as p.Val256-Ser261ValLys. The remaining 11 identified variants belonging to BRCA1 and BRCA2) are classified as polymorphisms or unknown variants.Conclusion:BRCA1 and BRCA2 mutations in females with breast cancer with positive family history of the disease is never low and cannot be neglected. Therefore screening for these mutations is important for strict follow up of those with positive results

خلفية الدراسة: كل من BRCa1 و BRCA2 هما مورثات كابتة للورم وتورث كصفة جسمية كابتة.يتراوح خطر العمر التراكمي للاصابة بسرطان الثدي الانتهازي لاولئك الذين يعانون من طفرات BRCA1 و BRCA2 من 53% الى 89% .يمثل سرطان الثدي العائلي اقل من 10% من جميع انواع سرطان الثدي , ولا تمثل السرطانات المرتبطة بمرض BRCA1 و BRCA2 سوى ارباع وثلثي الحالات. في النساء اللواتي تقل اعمارهن عن 35 سنة تمثل حوالي 10% الى 15% لطفرة BRCA1. ان النساء التي تعاني من التبدلات التي تنطوي على 21BRCA1 تتاثر بسرطان الثدي ولديهم خطر الاصابة بسرطان الثدي حوالي 52% الى 66%.اهداف الدراسة: هدفت هذه الدراسة الى التعرف على نوع الطفرة في اكسون 11 في جينات BRCA1 و BRCA2 في سرطان الثدي للنساء مع التاريخ العائلي الايجابي .المرضى والطرائق : هذه الدراسة مستقلة 11انثى مصابة بسرطان الثدي مع التاريخ العائلي الايجابي . اجريت الدراسة في مستشفى بعقوبة التعليمي على ثمانية اشهر (اكتوبر 2016-يوبيو 2017) كان مر المرضى04-70 سنة. تم استخلاص الحمض النووي من الخلايا اللمفاوية باستخدام عملية الاستخلاص .تم استخدام البرايمرات لتضخيم منطقة اكسون 11 من الجينات BRCA1 و BRCA2 باستخدام تفاعل البلمرة المتسلسل.النتائج: شوهد ثمانية تغايرات في جين BRCA1 و اربعة في جين BRCA2 تم الكشف عن الطفرة الجرثومية الضارة واحدة في جين BRCA1 في 141(7.14%). حيث كانت المريضة المصابة بالطفرة الضارة تبلغ من العمر 41 عاما وكان لديها تاريخ عائلي قوي للمرض (اثنان من الاقارب الاولى –الثاني ).حيث كان المتغير التسلسلي للطفرة C.795-789 delTT مع تأثير P.val 256-ser.261 valys. تصنف التغيرات الباقية التي تنتمي الى BRCA1 و BRCA2 على انها اشكال متعددة واشكال غير معروفة.الاستنتاجات : ان طفرات BRCA1 و BRCA2 في الاناث المصابات بسرطان الثدي مع التاريخ العائلي الايجابي للمرض ليست منخفضة ابدا ولا يمكن اهمالها. ومن ثم فحص هذه التغيرات ومتابعتها امر مهم لاولئك الذين لديهم نتائج ايجابية

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