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Article
Effect of Trichothececns toxin on stem cells isolated from Umbilical cord blood

Author: Hebat Alla Adel Abdulla AL-Hamdani1 , Mohammed Q.AL-Annie1 , Salah M.A.AL-Kubaisi2
Journal: Iraqi Journal of Biotechnology المجلة العراقية للتقانات الحياتية ISSN: 18154794 Year: 2018 Volume: 17 Issue: 3 Pages: 100-111
Publisher: Baghdad University جامعة بغداد

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Abstract

The study included the collection of umbilical cords blood samples immediately after delivery . Hematopoietic stem cells were isolated from the umbilical cord blood by the gradient density method. The hematopoietic stem cells (MNCs) were cultured in Dulbecco's Modified Eagle Medium (DMEM) with 10 % of fetal calf serum, then subjected to in vitro study to confirm the toxic effects of Trichothecenss extracts with the following concentrations (0.001,0.0039,0.0078, 0.0156, 0.031, 0.062, 0.13, 0.25, and 1 μg/ ml) and to detect the inhibition rate of hematopoietic stem cells by using 3-(4, 5-Dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide. The cytotoxicity assay (in vitro study), revealed that the inhibition rate increases seriously with the increase of Trichothecens concentration. Hence the concentration 1 μg /ml has given inhibition rate which reached 100%, while the concentration 0.001 μg /ml did not eliminates any type of cells. Trichothecens has a highly toxic effect on human hematopoietic stem cells, and its concentration is high and positively associated with the rate of cell death increases. Furthermore, the concentration 1 μg /ml of Trichothecens extracted from the Trichothecensgenic isolates can kill 100% of hematopoietic stem cell.


Article
Evaluation of some bioactive effect of phenolic compounds in Costus speciosus rhizome extract
تقييم تاثير بعض الفعاليات الحيوية للمركبات الفينولية في مستخلص جذور نبات القسط الهندي

Author: Zaid Akram Thabit زيد اكرم ثابت
Journal: Iraqi Journal of Science المجلة العراقية للعلوم ISSN: 00672904/23121637 Year: 2018 Volume: 59 Issue: 1A Pages: 38-43
Publisher: Baghdad University جامعة بغداد

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Abstract

This work was evaluated the antioxidant, antibacterial and cytotoxic activity of Costus speciosus rhizomes methanol extract. The FLC analysis showed the presence of five compounds in the methanol extract of C. speciosus rhizomes. These compounds were Quercetin (5.2mg/ml), Rutin (6.02mg/ml), Luteolin, (18.3mg/ml), Kaempherol, (11.34mg/ml) and Coumarin (1.41mg/ml). The maximum antioxidant activity of the extract was at concentration 1000 μg/ml with free radical scavenging activity approximately 67.5%. It was less than standard ascorbic acid 85.5% and Gallic acid 90% with significant difference (p≤0.05), with no significant difference in comparison with standard TBA 68.5%. The IC50 of extract was 3093μg/ml, while the IC50 of ascorbic acid, Gallic acid and TBA were (277.2, 364.5 and 601.3 μg/ml) respectively. The extract revealed influential growth inhibition for all bacteria used in this experiment. The extract was moderately effective at concentration 400 μg/ml of extract with inhibitory activity 50.7 % on MCF-7 cell line and IC50 139.1 μg/ml.

قيم هذا العمل تأثير المستخلص الميثانولي لجذور نبات القسط الهندي في فعاليته كمضاد للاكسدة وعلى البكتريا المرضية المختارة للدراسة وفعاليته السميه تجاه نوع من الخلايا السرطانية . تحليل FLC اظهر وجدود خمس مركبات ، وهي الكوارستين بتركيز 5,2 ملغم/مل والروتين بتركيز 6,02 ملغم/ مل واللوتين بتركيز 18,3 ملغم/مل والكامفيرول بتركيز 11,34 ملغم/مل والكومارين بتركيز 1,41 ملغم/مل. اعلى فعالية كمضاد للاكسدة كانت عند تركيز 1000 مايكروغرام / مل ، وبلغ نشاط الجذور الحرة حوالي 67,5% والتي كانت اقل من العقار القياسي حامض الاسكوربك 85,5% وحامض الكاليك 90% مع اختلاف معنوي عند (0,05≥P ) بينما لم يكن هناك فرق معنوي عند المقارنة مع TBA 68,5%. وكان IC50 للمستخلص 3093 مايكروغرام / مل ، بينما كان لحامض الاسكوربك ، وحامض الكاليك، و TBA ( 277,2 ، 364,5 و 601,3 مايكروغرام/ مل ) على التوالي.. اظهر المستخلص تأثيرا واضحا في تثبيط نمو كل انواع البكتريا المستخدمة في التجربة .كما اظهرالمستخلص فعالية سمية متوسطة وعند تركيز 400 مايكروغرام/مل وبفعالية تثبيطية 50,7% على خط الخلايا السرطانية MCF-7 ، و IC50 139,1 مايكروغرام / مل.


Article
Synthesis, Spectroscopic Characterization and in Vitro Cytotoxicity Assay of Mropholine Mannich Base Derivatives of Benzimidazole with Some Heavy Metals

Authors: Mahasin Alias --- Shaimaa R. Bakir
Journal: Al-Nahrain Journal of Science مجلة النهرين للعلوم ISSN: (print)26635453,(online)26635461 Year: 2018 Volume: 21 Issue: 3 Pages: 50-60
Publisher: Al-Nahrain University جامعة النهرين

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Abstract

Mannich bases derivatives of Benzimidazole were prepared from condensation reaction of 2-mercaptobenzimidazole with formaldeyhed once with mropholine and another with pyrazinamide to prepare L1 and L2 respectively, coordinated with three metal ions of Pd(II), Pt(IV) and Au(III). The structures of these compounds were confirmed by metal and elemental analyses, UV-Vis and FT-IR spectroscopy, magnetic susceptibility, conductivity measurement at room temperature, 1HNMR and 13CNMR. Experimental results showed that the ligands (L1&L2) coordinated as bi-dentate and tridentate with metal ions respectively. Cytotoxicity of Mannich bases and their metal complexes were examined against mice cell line RAW 264.7 using MTT method .Each cell line was injected by following doses (400,200,100 and 50) µg/ml of prepared compounds by using mice cell as a negative control and cis-platin as a positive control. The ligands and Pd(II), Pt(IV), Au(III) complexes showed good activity at various concentrations especially Pd(II) complexes of both complexes.


Article
Diagnosis of the Problems Which Facing the Researchers during Studying In vitro Lead Toxicity

Author: Sabah Hussain Enayah
Journal: Medical Journal of Babylon مجلة بابل الطبية ISSN: 1812156X 23126760 Year: 2018 Volume: 15 Issue: 1 Pages: 5-11
Publisher: Babylon University جامعة بابل

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Abstract

Background: Lead (Pb) is one of the most difficult metals to investigate in laboratory experiments because it is very easilyprecipitates or forms complex ions. Many experiments were conducted with Pb and observed unique chemical properties of this metal.Objective: The present investigation was designed to evaluate the cytotoxicity of Pb exposer on pheochromocytoma (PC12) cells indifferent condition and media to admit the difficulties facing the researchers in bioavailability of Pb in media and prevent precipitationformation. Materials and Methods: Pb from 0.01 to 100 μM had been used on PC12 cells cultured and treated in different conditions for 6,12, 24, and 48 h. Resazurin assay was used to assess the cell viability. Result: As numerous studies have demonstrated a possible mechanismfor Pb‑induced neurotoxicity using in vitro model, the current study is the first study which explained the complexity of Pb participations inmedia that facing the authors and what the reason for that and how we can solve this problem to make pb more bioavailable in the media.Conclusion: Use of phosphate buffer in experiments with Pb is not acceptable because all Pb can be precipitated. However, it could be possibleto use the standard media, but for the time of treatment with Pb phosphates must be removed from the media.


Article
Cytotoxic Activity of Taraxacum officinale Ethanolic Plant Extract against Human Breast Cancer (MCF-7) Cells and Human Hepatic (WRL-68) Cells

Authors: Rawa’a A. Muhammed --- Dhia S. Hassawi --- Nabeel K. Ibaheem
Journal: Iraqi Journal of Cancer and Medical Genetics المجلة العراقية للسرطان والوراثة الطبية ISSN: 20786123 Year: 2018 Volume: 11 Issue: 1 Pages: 16-21
Publisher: Al-Mustansyriah University الجامعة المستنصرية

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Abstract

Taraxacum officinale (Dandelion) represent a potentially novel non-toxic alternative to conventional cancer therapy towardmany cancers particularly breast cancer.This study was conducted to detect the cytotoxic activity of T. officinale whole plant ethanolic extract on human breast cancer(MCF-7) cells in vitro compared to human hepatic (WRL-68) cells using MTT assay.Graphed 6 prism program including non-linear regression with one –way ANOVA followed by Dennett’s multiple comparisontest to obtain data represent the mean+ SD.The results revealed high toxicity of the extract against MCF-7 cells with IC50 (190.5 μg.ml-1) and showed high-reducedactivity toward WRL-68 cells with high IC50 value while the lowest viability (21.57±5.322) appeared in MCF-7 cells undertreatment with 400 μg.ml-1 compared with high viability in WRL-68 cells.The study concluded that T. officinale extract has a valuable source of anticancer drugs with safe and selective activity oncancer cells in comparison to human normal cells.


Article
Activity of Peroxidase in Free Radicals,Human Blood Cells and Effect of the pH and Temperatureon Peroxidase Activity and Stability

Authors: Karrar R. Mohammed --- Entesar H. Ali
Journal: Journal of University of Babylon مجلة جامعة بابل ISSN: 19920652 23128135 Year: 2018 Volume: 26 Issue: 9 Pages: 108-115
Publisher: Babylon University جامعة بابل

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Abstract

The previously studiesof enzymes were focused only on the extraction and purification of enzyme and increase the toxic effect of drugs and supplement of free radical scavenging. So,the aim of thestudy wasto extend purified peroxidase from Pseudomonas aeruginosa application and to abundant new enzymatic drug of free radicals scavenging with low side effect. Peroxidase is categorized as oxido-reductases, whichwere used for catalyzing various oxidative reactions that protect cells from the harm effect of free radicals.Peroxidase was tested as antioxidant and test their toxicity on human cells to overcome the problem of the side effect of drug and supplement that used as an antioxidant. The enzyme was applied as H2O2 scavenging activity antioxidant by used three concentration of enzyme and triplicate (10, 40, 60 µg/ml).The enzyme was show higher activity at 60 µg/ml reached to 40% activity. The cytotoxicity of peroxidase was tested on whole healthy human blood by C.B.C by using equal amount 5µg of (enzyme substrate, only substrate and enzyme without substrate). The result showed that the enzyme only had no effect on human blood as compare with H2O2 and enzyme with H2O2. The characterization of enzyme was done by using more than one temperature and pH value and the result were shown that the optimal temperature for enzyme activity and stability rang (40-45°C) and the optimal pH for enzyme stability and activity were 7.

تركزت الابحاث السابقة للانزيمات على استخلاص وتنقية الانزيمات والمؤثرات الجانبية لاستخدام الادوية والمكملات الغذائية الكابحة للجذور الحرة. لذلك، الهدف من الدراسة هو توسيع تطبيقات إنزيم البيروكسيديز المنقى من الزوائف الزنجارية والقدرة على اكتشاف علاج انزيمي متوفر ورخيص الثمن وقليل التاثيرات الجانبيه. انزيم البيروكسيديز من الانزيمات المؤكسدة المختزلة والتي تعمل على تحفيز العديد من تفاعلات الاكسدة لتخليص الخلايا من خطر الجذور الحرة. اختبر البيروكسيديز في قدرته على كبح الجذور وسميته على خلايا الدم للإنسان لحل مشكلة الادوية والمكملات الغذائية. لذا استخدمت ثلاث تراكيز لانزيم البيروكسيديز(10,40,60 مايكروغرام/مل). حيث اظهر البيروكسيديز فعالية عالية في كبح الجذور الحرة عند تركيز (60 مايكروغرام/مل) وصلت الى 40.%درس تاثير الخلوي السام للبيروكسيديز على جميع خلايا الدم للانسان باستخدام جهاز عد الخلايا وباستخدام كميات متساوية من (الانزيم,المادة الاساس,الانزيم مع المادة الاساس) حيث لم يظهر الانزيم بدون المادة الاساس سوى تغيرا طفيفا باعداد الخلايا مقارنة بالمادة الاساس والمادة الاساس والانزيم. توصيف انزيم البيروكسيديز أجرى باستخدام أكثر من درجة حرارة واحدة وأكثر من اس هيدروجيني واحد حيث اظهرت النتائج ان أفضلاس هيدروجيني لفعالية الانزيم وثباتيته كانت عند اس هيدروجيني 7 وافظل درجة حرارة عند (40-45) درجة مئوية.

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