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Article
Modification of Ti Plasmid which Extracted from Agrobacteriumtumefaciens to Construction the dTi Vector for gene cloning
تحوير بلازميدTi المستخلص من بكتريا Agrobacterium tumefacies لتصنيع الناقل dTi والمستخدم في التنسيل الجيني

Author: Hisham Faiadh Mohammad هشام فياض محمد
Journal: basrah journal of science البصرة للعلوم ISSN: 18140343 Year: 2014 Volume: 32 Issue: 2B micro conf Pages: 194-205
Publisher: Basrah University جامعة البصرة

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Abstract

A total of 47 of infected potatoes in different size ( Solanumtuberosum) were collected from local vegetable and fruit market and from University of Basrah-Collage of Agriculture, Department of Plant Protection in addition to the three soil samples were taken from Basrah city. From which 22 (44%) isolates were isolation and identification including 19(86.4%) isolates from infected potatoes and 3(13.7%) isolates from soil. All isolates were identification and characterization by using biochemical, physiological and biotechinqicalinvestigation.The extracted DNA of Agrobacteriumtumefaciens isolates were subjected to PCR for amplifying 16S rRNA and for amplifying T-DNA fragment then subjected to gel electrophoresis. The individual band of the 16S rRNA gene was characterized by 1479bp and of the T-DNA fragment by 1200bp. The products were comparison with the standard molecular DNA ladder (1200 and 1500bp). The purified β-lactamase gene was cut from PGLO plasmid and ligated by use T4DNA ligase enzyme with the Ti plasmid which disarmed T-DNA by the same restriction enzyme. The result was indicated by using the E.coli K12 for carries the Ti plasmid vector which contain a β-Lactamase gene when put the antibiotic ampicillin in different consternation into the LB, MHA plate only the colonies which that have picked up exogenous DNA(dTi plasmid ) can grow that is mean it become resistance to ampicillin by using dTi vector

جمعت 47 عينة مختلفة الاحجام لبطاطا مصابة بمرض التورم التاجي من الاسواق المحلية للخضر والفواكة ومن جامعة البصرة كلية الزراعة ، قسم وقاية النبات بالاضافة الى ثلاثة عينات اخذت من التربة لمدينة البصرة. عزلت منها 22 عزلة شكلت (44%) شملت 19 عزلة (86.4%) من بطاطا مصابة بمرض التورم التاجي وكذلك 3 عزلات (13.7%) من عينات التربة. جميع العزلات شخصت وصنفت اعتماداً على الفحوصات البايوكيمياويةوالفسلجية والتقنية الاحيائية. الـDNA المستخلص لعزلاتAgrobacteriumtumefaciens عمل له (PCR) لتضخيم الجين 16S rRNA وكذلك تضخيم قطعة الـ T-DNA بعد ذلك عمل لها ترحيل كهربائي حددت الحزم لكل من T-DNAو 16S rRNAوقورنت مع مقياس قياسي وكانت اطوال الحزم هي 1474 زوج قاعدة لـ16S rRNA و 1200 زوج قاعدة لـ T-DNA مع مقياس يتراوح بين 1500و 1200 زوج قاعدة ، نقي الجين المسؤول عن صفة المقاومة للامبسلينβ-lactamase gene حيث تم قطعة من بلازميدPGLO وتم ربطة بواسطة انزيم الرابط T4DNA مع بلازميد(Ti) حيث قطع الاثنان بنفس الانزيم القاطع .وظهرت النتائج باستخدام عترة E.coli K12 القياسية لنقل الجين المسؤول عن مقاومة المضاد الحيوي الامبسلين المحمول على بلازميد (Ti)حيث عند اضافة المضاد الحيوي الامبسلين الى الوسط الزرعي (LB) لوحظ ان المستعمرات التي اخذت هذه الصفة فقط هي التي نمت في الوسط الزرعي الحاوي على المضاد الحيوي الامبسلين مما يعني انها اكتسبت صفة المقاومة للمضاد الحيوي الامبسلين.


Article
Biometric characteristic of the common goldfish Carassius auratus auratus (Linneaus, 1758) in Basra freshwater systems.
الخصائص الحياتية للسمكة الذهبية Carassius auratus auratus (Linneas, 1758) بأنظمة المياه العذبة في البصرة.

Author: F.Sh. Habbeb فوزية شاكر حبيب
Journal: Mesopotamian Journal of Marine Science مجلة وادي الرافدين لعلوم البحار ISSN: 18152058 Year: 2014 Volume: 29 Issue: 2 Pages: 155-161
Publisher: Basrah University جامعة البصرة

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Abstract

About 110 specimens of the goldfish Carassius auratus auratus (Linneaus, 1758) were obtained from the Department of Fisheries and Marine Resources Laboratory College of Agriculture and the Marine Sciences Center, University of Basrah. Morphometric and merestics characteristics were measured, total and standard length ranged between 90-150 mm and between 70-110 mm, respectively. Number of scales in the lateral line ranged from 25 to 27. Number of dorsal branched rays ranged from 14 to 15, gill rakers from 42 to 44. Total vertebrae fin count was 25-27. There were five rays with the branchuiostegal rays.

جمعت 110 نموذج من السمكة الذهبية من مختبرات قسم الأسماك والثروة السمكية ومن أحواض أسماك الزينة في محطة الاستزراع في مركز علوم البحار. أخذت القياسات المظهرية والعددية إلى أقرب ملم، إذ تراوح الطول الكلي بين 90-150 سم والطول القياسي بين 70-110 سم وتراوح عدد الحراشف على الخط الجانبي بين 25-27. تراوحت عدد الأشعة المتفرعة للزعنفة الظهرية بين 14-15 كما تراوح عدد الأسنان الغلصمية بين 42-44 والعدد الكلي للفقرات بين 25-27 وعدد الأشعة العظمية الغلصمية خمسة أشعة.


Article
MECONIUM ILEUS; A STUDY AND COMPARISON BETWEEN COMMON OPERATIVE PROCEDURES PERFORMED IN BASRAH

Author: Haithem H Ali Almoamin
Journal: Basrah Journal of Surgery مجلة البصرة الجراحية ISSN: 16833589 / ONLINE 2409501X Year: 2016 Volume: 22 Issue: 2 Pages: 84-90
Publisher: Basrah University جامعة البصرة

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Abstract

Abstract Meconium ileus accounts for 9–33% of all neonatal intestinal obstructions, with an incidence of 1:2500 newborns, representing the third most common cause of neonatal small bowel obstruction after atresia and malrotation. This study aimed to compare various surgical procedures used in the treatment of meconium ileus and to assess their efficacy regarding survival and complications. A retrospective study was done to all cases of meconium ileus admitted to the neonatal intensive care unit of Basrah hospital of maternity and children and Basrah children specialty hospital during the period of 10 years (2005 to 2015). The medical records of 57 cases of meconium ileus were studied. The comparison included: Mikulicz procedure, Bishop-Koop procedure and resection with primary anastomosis in both simple and complex meconium ileus. The parameters used for comparison were anastomotic leaks, high output diarrhea with dehydration and failure to thrive, sepsis, need for reoperation, wound complications, early adhesions, hospital stay and mortality. The mean age of presentation of neonates with meconium ileus was 3.9 days. Male to female ratio was 0.9: 1. About 10.5% were preterm. About 33.3% of cases were diagnosed as simple meconium ileus. Intestinal Volvulus is the predominant complications encountered (47.4%). Non-operative treatment was effective in 45.5%. The most common procedures done in our center were Mikulicz procedure (61.5%), followed by Bishop-Koop procedure (30.8%), and resection with primary anastomosis (7.7%). There was a significant association between mortality and high output fistula, anastomotic leaks, sepsis, and reoperation. Predominant complications in Mikulicz procedures were high output fistula (50%) and skin excoriation (53.1%), while in Bishop-Koop procedure were sepsis (75%), reoperation (50%), and adhesions (25%). In primary anastomosis, significant complications were anastomotic leak (75%), sepsis (50%), and reoperation (50%). Mortality was highest in primary anastomosis (75%), followed by Bishop-Koop procedure (62.5%), and lowest with Mikulicz procedure (40.6%). The overall mortality of meconium ileus was high 45.6% (42.9% for simple meconium ileus and 52.6% for complex meconium ileus). All neonates treated non-operatively survived, while the survival rate for those treated surgically was 50%. In conclusion, resection with stoma creation is superior to primary anastomosis. Mikulicz procedure is the safest procedure to be done with best survival and less complications. Bishop- Koop procedure is of value in a situation where the surgeon is afraid from high output diarrhea so proximal stoma is mandatory.

Keywords

MECONIUM ILEUS --- BASRAH


Article
STUDY OF SOME BLOOD NORMAL PARAMETERS OF SHEEP IN BASRAH
دراسة بعض معايير الدم الطبيعيه في الاغنام في البصره

Author: Hassanin Husham Naser Al-Autaish
Journal: Basrah Journal of Veterinary Research. مجلة البصرة للابحاث البيطرية ISSN: Print:18138497 E; 24108456 Year: 2016 Volume: 15 Issue: 2 Pages: 380-385
Publisher: Basrah University جامعة البصرة

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Abstract

This study was conducted in Basrah at period from September- 2012 throughoutAugust- 2013.Blood samples were collected from 480 sheep in different ages andboth genders. Haematological parameters were estimated and the result revealed that;the sheep included in this study have lower haematological parameters than thestandard ones. Young animals in this study suffering from macrocytic normochromicanaemia

Keywords

sheep --- anemia --- Basrah


Article
DUODENAL OBSTRUCTION IN CHILDREN: AN EIGHT YEAR EXPERIENCE IN BASRAH

Author: Abbas Abdulzahra Alhasani
Journal: Basrah Journal of Surgery مجلة البصرة الجراحية ISSN: 16833589 / ONLINE 2409501X Year: 2017 Volume: 23 Issue: 2 Pages: 62-68
Publisher: Basrah University جامعة البصرة

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Abstract

DUODENAL OBSTRUCTION IN CHILDREN: AN EIGHT YEAR EXPERIENCE IN BASRAHAbbas Abdulzahra AlhasaniMB, ChB, FIBMS, MRCS Glasgow, Pediatric Surgeon, Lecturer, Department of Surgery, College of Medicine, University of Basrah, IRAQ.Abstract Duodenum is a common site for congenital anomalies that might result in partial or complete obstruction. Intestinal rotational anomalies that involve the midgut put the duodenum at a potential risk of obstruction. This is a retrospective hospital record based study displaying an 8 year single center experience in the Basrah center of pediatric surgery. The study enrolled 60 children with congenital duodenal obstruction with a mean age of 2.8 months and a male to female ratio of 1.14:1. Thirty two patients (53.3%) were diagnosed as duodenal atresia and stenosis, while intestinal malrotation was reported in 28 patients (46.7%). Repeated vomiting was the most common presenting symptom, it was bile stained in 60%. Associated congenital anomalies were seen in 23.3% of the study population, Down's syndrome and congenital heart defects were the most commonly reported abnormalities. Intraoperatively, fenestrated duodenal web was the commonest finding (59.4%) in duodenal atresia/stenosis, which was corrected by a diamond anastomosis in 1.9% of cases and by duodenotomy with web excision in 28.1%. Malrotation was corrected by Ladd's operation in 92.9%. Postoperative complications were reported in one third of the studied population, and the type of surgical procedure used in duodenal atresia/stenosis did not have a significant impact neither on postoperative complications nor on the hospital stay.


Article
17- PREVALENCE AND ANTIBIOTIC SENSITIVITY OF Escherichia coli AND Klebsiella pneumoniae FROM PATIENTS AND ANIMALS IN BASRAH PROVINCE

Author: Roaa A. Sabeeh*, Mazin N. Mousa**, Bassam Y. Khudaier
Journal: Basrah Journal of Veterinary Research. مجلة البصرة للابحاث البيطرية ISSN: Print:18138497 E; 24108456 Year: 2018 Volume: 17 Issue: 1 Pages: 192-208
Publisher: Basrah University جامعة البصرة

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Abstract

During the period of seven months from October 2016 to May 2017, 299samples were collected, 152(51%) human samples, of which 69 (45.4%) were fromurine and 83 (54.6%) were from children suffering from diarrhea in hospitals in AlBasra governorate. 147 (49.2%) samples were from fecal of animals, of which 82(55.8% ) samples were from buffalo and 65(44.2%) were from cow .A total of 101E. coli serotype O157: isolates out of 299 were suspected E. coli analyzed 52/101(34.2%%) were from human 16 (10.5%) samples were from urine and 36 (23.7%)samples were from stool . and 49/101(33.3%) were from animal 33(22.4%)samples were from buffalo and 16 (10.9%) samples were from cow . On the otherhand 68(22.7%) isolates out of 299 were suspected K. pneumoniae analyzed 41/68(27%)were from human 28/41( 18.4%) isolates were from urine samples and 13/41(8.6%) isolates were from stool samples and 27/68 (18.4%) were from animal 16(10.9%) samples were from buffalo and 11(7.5%) samples were from cow).Allsuspected isolates were subjected to testing biochemical. It was found that 10 out of101 were 4 isolates of animal faeces (2 buffalo and 2 cows).six isolates of 52isolates (4 of the children's stool samples and 2 of the urine samples) 19.5% nonfermented sorbitol (NSFEC).The isolates were tested against 14 different antibiotics

Keywords

Escherichia coli --- patients --- Basrah.


Article
Self-reported hand contact dermatitis among female hairdressers in Basrah
التهاب الجلد التماسي المعلن عنه ذاتيا بين النساء العاملات بمهنة الحلاقة في البصرة

Author: Wasan H. Ghani Asaad Q. Al-Yassen
Journal: The Medical Journal of Basrah University المجلة الطبية لجامعة البصرة ISSN: 02530759 Year: 2018 Volume: 36 Issue: 2 Pages: 60-65
Publisher: Basrah University جامعة البصرة

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Background: Occupational skin diseases are common and deserve medical attention. Hairdressers are special risky group for skin diseases.Objective: To determine the prevalence of self-reported hand contact dermatitis among female hairdressers in Basrah city.Methods: Sixty one salons in Basrah city center were chosen randomly by using the systematic random sampling technique from a name’s list of registered salons in Basrah General Health Directorate. A convenient sample of female hairdressers was chosen from these salons.A special questionnaire form prepared for the purpose of the study was used for collection of data from female hairdressers who agreed to participate in the study by direct interview.Results: A total of 80 female hairdressers were included in the study. Their age ranged between 19 and 65 years old with a median age of 32.5 years. The prevalence of self- reported hand contact dermatitis or eczema among female hairdressers was 31.3%. It was significantly associated with the age and nationality of female hairdressers (P-value = 0.021 and 0.035 respectively). Furthermore, there was significant association between self- reported hand contact dermatitis and personal history of atopy (P-value < 0.001) and family history of atopy. (P-value=0.031).Conclusions: The self- reported hand contact dermatitis is fairly common and associated significantly with age of female hairdressers, nationality, personal and family history of atopy. We recommend to enhance occupational health measures for the hairdressers with special alteration in the preventive aspects

الخلفية: الأمراض الجلدية المهنية شائعة وتستحق الرعاية الطبية العاملين بمهنة الحلاقة هم اكثر عرضة للإصابة بالأمراض الجلديةالهدف من الدراسة: معرفة مدى انتشار التهاب الجلد التماسي لدى العاملات في مهنة الحلاقة في محافظة البصرةطريقة العمل: اشتملت هذه الدراسة على 61 صالون في مركز مدينة البصرة اختيرت عشوائيا من قائمة اسماء الصالونات المسجلة في دائرة صحة البصرة.نتائج الدراسة: العدد الاجمالي للعاملات في مهنة الحلاقة في هذه الدراسة كان 80 عاملة. العاملات اللواتي تضمنتهن الدراسة تراوحت اعمارهن بين 19 و 65 عام وكان متوسط اعمارهن 32.5 عاما. وكان هناك ارتباط معتد احصائيا بين معدل انتشار التهاب الجلد التماسي لدى العاملات في مهنة الحلاقة و عمر العاملة) على التوالي. P- value = 0.021 & 0.035) وجنسيتها وكذلك يوجد ارتباط معتد احصائيا بين نسبة انتشار التهاب الجلد التماسي ووجود التهاب الجلد الاتوبي او في التاريخ العائلي لدى العاملات في مهنة الحلاقة.0.031) (P-Value < 0.001 & على التوالي. الاستنتاجات: نستنتج من هذه الدراسة ان التهاب الجلد التماسي يرتبط بشكل كبير بسنّ العاملات في مهنة الحلاقة وجنسيتهن والتاريخ الشخصي والعائلي للإصابة بالتهاب الجلد الاتوبي. من المهم تعزيز واقع الصحة المهنية للعاملات في مهنة الحلاقة.

Basrah Journal of Surgery
مجلة البصرة الجراحية

ISSN: 16833589 / ONLINE 2409501X
Publisher: Basrah University جامعة البصرة
Subject: Surgery

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Abstract

Basrah Journal of Surgery Editor-in- chief Prof. Thamer A. Hamdan, FRCS Prof. Salam N. Asfar, MSc Information Administrator Dr. Jasim M. Salman, MB,ChB, DA, FICMS Associate Editors Dr. Hashim S. Khayat, FRCS Prof. Mazin H. Al-Hawaz, CABS, FRCS Prof. Zeki A. Al-Faddagh, CABS, FRCS Prof. Issam Merdan CABS, FICMS Assist. Prof. Ali A. Alshawi, FFDRCSI, FDSRCS Assist. Prof. Mazin Abdulsattar, CABS Dr. Zuhair Al-Barazanchi, MSc, PhD National Advisory Board Prof. Ahmad M Al-Abbasi, FRCS Prof. Abdulla M. Jawad, PhD Dr. Hasan K. Muhamed, FRCS Assist. Prof. Tahir A Hawrami, DS, CABS Assist. Prof. Farhad K. Sulayvani, CABS Prof. Hassan J Hasony, MPhil, PhD Prof. Nadhim K. Mahdi, PhD International Advisory Board Dr. Majeed H Alwan, FRCS, New Zealand Dr. Luay P Susan, MD, Cleveland, Ohio, USAProf. Stewart L Weinstein, MD, USA, Former president AAOSDr. Robert W Buchoiz, MD, USA, Former President AAOSDr. Cody Bünger, MD, USA, President SICOTDr. Scott A Hoffinger, MD, Oakland, CA, USADr. Sebastian Gitter, Dr.med. Biberach, GermanyDr. Alec Benjamin, FRCS, UKProf. W Al-Ma’ani, MD, JordanProf. K Fathie, MD, FACS, USAProf. Hikmat Jamil, MD, PhD, USAProf. Wajdy L Haillo, MD, PhD, USAMr. David Gallaway, PhD, FRCSDr. Rick Wilkerson, MD, USADr. Sabri Shukur, FDSRS, USAProf. Gary Selnow, USADr. John Howe, USADr. Karim A Shaikley, MD, USADr. John Chalmers, FRCS, UKDr. J Frazer, MD, USADr. M Zayer, MD, SwedenDr. S Al-Bodur, MD, Jordan Administrative Secretary Ms. Elham AltomaMs. Salima J Sa’adInstructions to AuthorsBasrah Journal of Surgery publishes original articles, review articles, leading articles and case reports; all manuscripts are submitted to editorial review. Authors are requested to send two copies of their articles and other editorial material to:The EditorBasrah Journal of Surgery, Dept. of Surgery, College of Medicine, University of Basrah. IRAQ.salamasfar@yahoo.com OR basjsurg95@yahoo.com An article is reviewed for publication on the understanding that the work it reports has not been submitted simultaneously to another journal, has not been accepted for publication by another journal, has not been accepted for publication by another journal nor has it been already published. Any such attempt, when detected, will result in automatic rejection and may prejudice acceptance of future contributions. The articles and their illustrations become property of the journal. The editorial board does not necessarily agree with views expressed by the authors. A covering letter must accompany all submissions and must be signed by all authors. The first named author is responsible for ensuring that all authors have seen and approved the manuscript and are fully conversant with its contents. Authors should consult “uniform requirements for manuscripts submitted to biomedical journals” produced by the international committee of medical Journal Editors and published in the British Medical Journal, 6 February 1988, 401-5. Only manuscripts, which comply with this, the Vancouver style, will be considered. The text of the articles is usually divided into sections with the headings, Summary (abstract), Introduc-tion, Methods, Results and Discussion. Long articles may need subheadings within some sections to clarify its content. Two good quality plain paper copies of all written and tubular material should be submitted. The manuscript must be typed double-spaced with a minimum of 3cm margins on A4 paper (210 × 297 mm) in the following order: 1.Title page with name of the author(s). The appointment of each author at the time of performance of the work reported should be mentioned; if an author has since moved then his new address is to be added as footnote. Authors names should be mentioned up to six, if there are more authors then the first three are mentioned followed by “et al.”.2.Summary should be concise, complete in itself and outline the aim, results and conclusions of the paper. Randomized controlled trials should be identified.3.The text of all articles should be short and to the point.4.References should be numbered according to their sequence in the text and not alphabetically. They should be written down in compliance with Vancouver style; i.e.A)Journal: Ferguson AJ, Mazier WP, Ganchrow MI, Friend WG. The closed technique of haemorrhoidectomy. Surgery 1971; 70: 480.B)Book: Golgberg SM, Nivato-vongs S, Rothenberger DA, Colon, Rectum and Anus. In: Schwartz SI, Shire GT, Spencer FC, eds. Principles of surgery. 4th ed. Singapore: MacGraw Hill; 1984.5.Tables should be separately typed each on one sheet and must have a Roman identifying number and a short descriptive title. 6.Illustrations should be submitted in duplicate. Photographs must be of the highest professional quality. Original line drawings may be sent instead of photographs. Never write on the front or back of drawing or photographs.7.Pagination, all pages should be numbered in the sequence, title page, summary, text, acknowledgment, references, tables and legends for illustrations.8.Abbreviations should be limited in the text. Terms, which are mentioned frequently, may be abbreviated, and clearly defined on first use. 9.Statistical analysis should include a clear description of which methods were used for which analysis. Results of statistical tests should be reported by stating the value of the test statistic, the number of degrees of freedom and the P value. For example, t=1.34, 16 d.f., p=0.2. There should also be an indication whether the results were statistically significant or not.10.It is preferable to provide the manuscript on a compact disc using MS Word 2003 version.Checklist for authors1-Covering letter.2-Two copies of the article.3-Writing style according to the Vancouver style.4-Tables, graphs and legends for the photos each on separate sheet of paper.5-A copy of the article on a compact disc. AnnouncementThe editorial board would like to inform our readers that the journal has been included in the Index Medicus for the WHO Eastern Mediterranean Region (IMEMR) starting from the March, 1999 issue (Vol.5, No.1). This issue as well as all forthcoming issues of the journal will appear in WHO/EMRO web site on the Internet at the address http://www.who.sci.eg The journal was also included in the collection of the National Library of Medicine, Bethesda, Maryland starting from the same issue (i.e. Vol.5, No. 1) as we were informed by the National Institutions of Health in their letter dated February 2, 2000. Website: http://basjsurge.com email: basjsurg95@yahoo.comMobile: 009647801061850 Editor 009647801018133 Information Administratorمجلة البصرة الجراحية 2015

مجلة البصرة الجراحية تصدر عن كلية الطب جامعة البصرة تعنى بنشر البحوث الطبية.

Keywords

Basrah --- Journal --- Surgery


Article
ANNOTATED CHECKLIST OF REPTILIAN FAUNA OF BASRAH, SOUTH OF IRAQ
قائمة مرجعية للزواحف في محافظة البصرة, جنوب العراق

Authors: Hind D. Hadi وهند ضياء هادي --- Azhar A. Al-Moussawi أزهار احمد الموسوي --- Saman R. Afrasiab سامان روستم افراسياب
Journal: Bulletin of the Iraq Natural History Museum مجلة متحف التاريخ الطبيعي العراقي ISSN: Print ISSN: 10178678, Online ISSN: 23119799 Year: 2018 Volume: 15 Issue: 1 Pages: 77-92
Publisher: Baghdad University جامعة بغداد

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Abstract

Basrah province is situated at the extreme south of Iraq, it has an interesting reptile fauna (Squamata and Serpentes) and represents a land bridge between three different zoogeographical regions ( Oriental, Palaearctic and Ethiopian). This situation gave Basrah province a topographic specific opportunity for raising its own faunal diversity including reptiles; in this study Basrah province was divided into four main zones: the cities and orchards, marshes and wetlands (sabkha), the true dessert, the seashore and Shat Al-Arab.Forty nine reptile species were recorded including snakes, sea and fresh water turtles, and Lizards; brief notes and descriptions for the rare and important species were provided and supported by Plates.

تقع محافظة البصرة في أقصى جنوب العراق وتمتلك ميزة خاصة في تنوع الزواحف (العضايا والحيات) وهي تشبه جسر بين ثلاثة مناطق حيوانية-جغرافية عالمية ( الشرقية، القطبية القديمة و الاثيوبية)، أعطى هذا الموقع لمحافظة ألبصره خصوصية في التنوع الإحيائي ومن بينها الزواحف. من اجل تسهيل الدراسة قسمت محافظة البصرة إلى أربعة مناطق رئيسة و هي: المدينة والبساتين، الأهوار والمستنقعات، الصحراء الحقيقية و ساحل البحر وشط العرب. استعرضت الدراسة تسجيل 49 نوعاً من الزواحف تتضمن الحيات وسلاحف المياه العذبة و البحرية والعضايا. أعطي الوصف واهم ألملاحظات التصنيفية لبعض الأنواع النادرة معززا بالصور.


Article
ISOLATION AND IDENTIFICATION OF Staphylococcus aureus FROM TICKS ON THE CATTLE IN BASRA CITY
عزل وتشخيص بكتريا المكورات العنقودية الذهبية في القراد المتطفل على الماشيه في مدينة البصره

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Abstract

Ticks are an important external parasite have a mechanical affect through an imbibing blood of the host and transferring a pathogenic bacteria or a virus. The present study was focusing on the isolation of bacteria from the ticks parasitized cattle in north of Basra city,This study was conducted in north of Basrah (Qurna district) from February to July 2017Atotal number of 80cattle parasitized with ticks were samplesIsolated ticks were identified as Hyalomma to recognize what types of bacteria might be existed in these ticks, some of the cultures and biochemical tests were used. As a consequence, Staphylococcus aureus being identified in 14 of cattle sampled that infected with ticks. Regarded to present study, Staphylococcus aureusis one of the most pathogenic bacteria that can have an impact on an animal health production and this study that conducted from little studies in Iraq especially in Basrah about diagnosis of bacterial infection from Ticks.

يعد القراد من الطفيليات الخارجية المهمةوالتي لها تأثيرات ميكانيكية من خلال مص دماءالمضيف وكذلك لها اهميه فينقل البكتيريا المرضية أو نقل الفايروس. وقد تركزت دراستناعلى عزل البكتريا من القراد المتطفل على الماشية في شمال محافظة البصرة اجريت هذه الدراسة في قضاء القرنة ابتداء من شهر شباط الى نهاية شهر تموز ,إجمالي عددالأبقار التي اخذت منها العينات والمصابة بالقرادهي 80 .شخص القراد المعزول بانه تابع الى جنسHyalomma..البكتيرياالتي شخصت في هذا القراد بعد استخدام بعض الاوساط الزرعية والاختبارات الكيميائية الحيوية هي المكورات العنقودية الذهبية والتيتم تحديدها في 14 من الماشية المصابة بالقراد. تعتبر دراستنا والتي من خلالها تم تشخيص المكورات العنقودية الذهبية في القراد تعتبر واحده من معظم انواع البكتيريا المسببة للأمراض والتي يكون لهاتأثير على صحة الحيوان من خلال الاصابة الثانوية ولها تأثير من جانب صحة اللحوم

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