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Article
HLA-DR and DQ Antigens in Iraqi Patients with Behget's Disease

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Abstract

Summary:Background: Behqet's disease (BD) is a multisystemic in inflammatoty disorder, disease onset is believed to be triggered by many factors with a particular genetic background. The aim of this study is to investigate the possible correlation between HLA-DR & DQ antigens and onset of this mysterious disease.Method: By using microlymphocytoxicity test, the frequencies of 18 HLA-DR & DQ antigens were calculated in 65 patient with BD compared with 32 patients control with recurrent oral ulcers (ROU) & 115 healthy control.Results: Significant increased trend of HLA-DR52, DR53 and DQ3 antigens (P<0.001), and significant reduced frequencies of DQ4 antigen (P<0.01) in patient with BD as compared to H.C. group, on the other hand significant decreased DR1 antigen in patient with BD (P<0.005) as compared with patients with ROU.Conclusion: Higher frequency of DR52, DR53 & DQ3, these alleles could in one way or another play a crucial role in susceptibility to BD. Whereas DR1 DQ4 phenotypes decrease the risk to develop, this disease.Key words: Behget's disease, HLA- typing HLA-DR and DQ.


Article
Human Leukocyte Antigen-DP in Acute Nonlymphocytic and Acute Lymphoblastic Leukemia

Author: Majid Sakhi Jabir
Journal: Iraqi Academic Scientific Journal المجلة العراقية للاختصاصات الطبية ISSN: 16088360 Year: 2006 Volume: 5 Issue: 3 Pages: 285-288
Publisher: The Iraqi Borad for Medical Specialization المجلس العراقي للاختصاصات الطبية

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Abstract

ABSTRACT:BACKGRAOUND:Acute leukemias are characterized by the elonogenic proliferation of hematopoietic progenitor cells arrested in maturation .Clinical manifestations are related to the infiltration of the bone marrow and other tissues by the leukemic blasts.METHODS:Peripheral blood mononuclear cell (PBMC) from leukemic patients (29)(ANLL),(36)(ALL) and from normal donors(55)were typed for Human leukocyte antigen-DP specificities using microlymphocytotoxicity assay.RESULTS:Results showed frequencies of (DPw) alleles in (ANLL) patients were not significantly different from controls ,exept that in DPw1 was absent .In contrast ,in ALL,frequencies of DPw2 and DPw5 were significantly increased (p< 0.05,relative risk (RR)= 1.95 and p<0.01,RR=4.27,respectively .CONCLUSION:These results ,demonstrate both positive and negative associations between major histocompatibility complex (MHC) gene products which are in only very weak linkage with the rest of HLA ,and (ALL,ANLL) patients.The HLD-DP region could thus contain long sought –after genes influencing susceptibility and resistance to leukemogenesis.

Keywords

ANLL --- ALL --- HLA --- DP-Antigen.


Article
Genetic characteristics and β-cell Autoimmunity in T1DM Children

Author: Eman M. Saleh
Journal: Journal of the Faculty of Medicine مجلة كلية الطب ISSN: PISSN: 00419419 / EISSN: 24108057 Year: 2007 Volume: 49 Issue: 4 Pages: 414-424
Publisher: Baghdad University جامعة بغداد

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Abstract

Background: TIDM is known to be polygenic disease that appears from the interaction of mutation in multiple genes including HLA. The autoimmune mediated destruction of pancreatic β-cells is reflected by the presence of autoantibodies against prominent antigens in the pancreatic β-cells. Objective: This study was designed to investigate the role of HLA-class I and class II antigens in the etiology of type 1 diabetes mellitus (T1DM) and also assessment of glutamic acid decarboxylase (GAD65) autoantibodies in the patients at the onset of the disease. Patients & Methods: Sixty T1DM patients who were newly onset of the disease (diagnosed less than five months) were selected. Eighty apparently healthy control subjects, matched with age, sex and ethnic backgrounds underwent the HLA-typing by lymphocytotoxicity assay. Finally 50 healthy individuals were selected randomly to undergo serological assessment of GAD65 autoantibodies using IRMA method. Results & Conclusion: At HLA-class I region, T1DM patients showed a significant increased frequency of antigen A9 (40.0 vs.18.75%) and B8 (28.33 vs.8.75%) as compared to control subject. At HLA-class II region, DR3 and DR4 were significantly increased in patients (53.33 vs.26.25% and 50.0 vs. 12.5% respectively) as compared to controls. In addition to that, T1DM was significantly associated with DQ2 (33.33 vs.15%) and DQ3 (40.0 vs. 20%) antigens as compared to controls, suggesting that these haplotypes had a role in disease susceptibility, while the frequency of DR2 and DQ1 antigens were significantly lowered in patients compared to controls (6.66 vs. 25% and 6.66 vs. 22.5% respectively). These molecules might had protective effect. Anti-GAD65 autoantibodies were present in 50% of T1DM children especially in older ages and in females more than males. High proportion of GADA was found in the patients carrying HLA-DR3/DR4 heterozygous. In conclusion, susceptibility to T1DM is genetically controlled.


Article
HLA Class I and Class II Polymorphisms and Anti-nuclear Antibodies in Hyperprolactinaemic Iraqi Females with Primary Infertility

Author: Ali H. Ad'hiah
Journal: Journal of the Faculty of Medicine مجلة كلية الطب ISSN: PISSN: 00419419 / EISSN: 24108057 Year: 2008 Volume: 50 Issue: 4 Pages: 475-479
Publisher: Baghdad University جامعة بغداد

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Abstract

Background: The study was conducted to investigate the association between hyperprolactinaemia and markers of human leukocyte antigen (HLA) system in a sample of Iraqi infertile females, together with the profile anti-nuclear antibodies (ANA). Objectives: One hundred and seventy five female patients (age range: 20 -40 years) were recruited in this study. They were attending the Institute for Embryo Research and Infertility Treatment (Al-Nahrain University) during the period January 2005 - September 2006. Results:After clinical and laboratry evaluations, it was found that 100 patients were hyperprolactinaemic, whereas the other 75 patients were euprolactinaemic, therefore, they were considered as a control group. Based on serum level of prolactin (22-29, 30-39 and ≥ 40 ng/ml), the total hyperprolactinaemic patients were divided into three groups; I (35 patients), II (40 patients) and III (25 patients), respectively. The HLA antigens showed significant variations between patients (total and groups) and controls. In total patients, B8 (25.0 vs. 9.3%), DR3 (48.0 vs. 17.3%) and DR4 (39.0 vs. 13.3%) showed significant increased frequencies, while B35 showed a significant decreased frequency (7.0 vs. 24%). The latter decrease was also observed (5.7 vs. 24.0%) in group I of patients, which also showed a significant increased frequency of DR3 (54.3 vs. 17.3%). In groups II and III of patients, only DR3 (45.0 and 56.0, respectively vs. 17.3%) and DR4 (37.5 and 56.0, respectively vs. 13.3%) showed significant increased frequencies. Autoantibody evaluation by ANA test revealed that 22% of the total patients was positive, while all control subjects were negative, and such positivity paralleled the increased level of serum prolactin.


Article
Frequency of HLA-A and B Antigens in Iraqi Patients with End-Stage Renal Disease Preparing for Transplantation

Authors: Lazem H. Al-Taie --- Batool H. Al-Ghurabi --- Ahmed A .Al-Hassan --- Abid J. Dage
Journal: Iraqi Academic Scientific Journal المجلة العراقية للاختصاصات الطبية ISSN: 16088360 Year: 2012 Volume: 11 Issue: supplement Pages: 642-648
Publisher: The Iraqi Borad for Medical Specialization المجلس العراقي للاختصاصات الطبية

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Abstract

ABSTRACT:BACKGROUND:The likelihood of developing end-stage renal disease in an individual is determined by interactions between genetic and environmental factors. Human leukocyte antigen is the most polymorphic genetic system in man. Genes of this region influence susceptibility to certain diseases.OBJECTIVE:The purpose of the present study is to investigate the association of HLA class I (HLA-A and HLA-B) with the end-stage renal disease in Iraqi patients (Arab and Kurd).SUBJECTS AND METHODS:HLA-typing was assessed in 200 patients with end-stage renal disease and 110 healthy controls by microlymphocytotxicity assay.RESULTS:A survey of the distribution of HLA-A and HLA-B antigens frequencies yielded a significant variation between patients and healthy control group. Arab patients have significant increase in frequency of HLA-A2 as compared with healthy control (P=0.005). Whereas Kurdish patients revealed significant increase in frequency of HLA-B35 when compared with healthy control (P=0.033).CONCLUSION:The current study suggests that high frequency of HLA-A2 in Arab patients and HLA-B35 in Kurdish patients might be associated with susceptibility to risk of end-stage renal disease


Article
Association of HLA (Class I & II) and Susceptibility to

Author: Ikbal Kh. Al-Joofy Ph.D* د. اقبال الجوفي
Journal: Journal of the Faculty of Medicine مجلة كلية الطب ISSN: PISSN: 00419419 / EISSN: 24108057 Year: 2007 Volume: 49 Issue: 2 Pages: 265-266
Publisher: Baghdad University جامعة بغداد

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Summary:Background: Hydatid cyst disease is a parasitic zoonotic disease caused by genus Echinococcus. This disease believed to has genetic background in it's aetiopathogenesis course . The aim of this study is to shed light on the possible correlation between HLA-class I (A,B,C) & HLA-class II (DR & DQ) antigens and the susceptibility to this disease. Patients & Methods: Fifty patient with hydatid cyst disease before undergoing surgical operation were investigated for HLA. Class I and class II by using microlymphocytotoxicity test. The results were compared with 115 healthy control. Results: Significant increased trend of HLA-A28 and A-11, -B18 and B-35, -DR3 and DR-11 (P<0.001, P<0.01) in patients with this disease as compared with healthy control. On the other hand, increased trend of HLA-28 (P<0.01) in patients with hydatid disease especially in those with a cyst location in liver. Conclusions: High frequency of HLA-A11 and –A28, B18 and –B35, -DR3 and –DR11 antigens may play major role in susceptibility to hydatid disease. HLA-A28 could be the most related antigen to this disease and acting as genetic marker that could in one way or another play crucial role in susceptibility especially in cases of hydatid disease in liver. Keywords: Hydatid cyst disease, HLA-typing


Article
HLA-B genotype and Escherichia coli association in Iraqi patients with reactive arthritis
العلاقة بين النمط الوراثي لمستضد التوافق النسيجي نوع ب وبكتيريا القولون في المرضى العراقيين المصابين بإلتهاب المفاصل التفاعلي

Authors: Emad Resen Galeef عماد رسن جليف --- Dunya Fareed Salloom دنيا فريد سلوم
Journal: Iraqi Journal of Science المجلة العراقية للعلوم ISSN: 00672904/23121637 Year: 2016 Volume: 57 Issue: 3A Pages: 1648-1652
Publisher: Baghdad University جامعة بغداد

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Reactive arthritis (ReA) has been as joint developing after infection, it belongs to spongylo arthritis (SpA). The etiology of this disease was multi factorial, the combination between genetic and environmental factors for triggering this disease. This study included 75 Iraqi Arab patients and 39 healthy control. Urine samples and blood were collected from each subject. The results showed that Escherichia coli bacteria (E. coli) was isolated from 32% of urine samples. HLA-B*27 allele frequencies was higher in ReA patients infected with E. coli. This lead to suggest that E. coli may be trigger factor in ReA patients with UTI which had HLA-B*27 positive.

يعد إلتهاب المفاصل التفاعلي من الأمراض التي تصيب المفاصل والتي تحدث نتيجة للإصابة، حيث يعود الى عائلة الأمراض المرتبطة بإلتهاب المفاصل. إمراضية المرض تشترك فيها عدة عوامل متمثلة بالأرتباط ما بين الوراثة والبيئة التي تحث على المرض. تضمنت الدراسة 75 مريض عراقي و39 شخص أصحاء أستخدموا كسيطرة. جمعت عينات الأدرار وعينات الدم من مجاميع الدراسة، وأشارت النتائج الى أن نسبة بكتيريا القولون 32% حيث عزلت من أدرار المرضى وكان الليل HLA-B*27 أكثر ترددا في المرضى وكانت له علاقة في الأشخاص المصابين ببكتيريا القولون، لذا يمكن إعتبار بكتيريا القولون عامل محث لمرض إلتهاب المفاصل التفاعلي في الأشخاص المصابين بإلتهاب المجاري البولية


Article
Association between HLA-Class II Alleles and T-Cell Proliferation in Response to Enterovirus and Adenovirus

Author: Eman M. Saleh
Journal: Iraqi Academic Scientific Journal المجلة العراقية للاختصاصات الطبية ISSN: 16088360 Year: 2007 Volume: 6 Issue: 2 Pages: 102-108
Publisher: The Iraqi Borad for Medical Specialization المجلس العراقي للاختصاصات الطبية

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Abstract

BACK GROUND:Viruses may be involved in the pathogenesis of Type 1 Diabetes Mellitus (T1DM), either through direct β-cell infection or as triggers of autoimmunity.OBJECTIVE:To evaluate the T- cell proliferation in response to Enterovirus antigens including Coxsackievirus B and Poliovirus in addition to Adenovirus in an HLA- matched population of children with T1DM and children who were healthy.METHODS:A total of 60 Iraqi T1DM children were included in the presents study. They were new onset of the disease. For the purpose of comparisons, 50 apparently healthy control subjects were selected. HLA typing was measured by microlymphocytotoxicity, while methylthiazoltetrazolium (MTT) assay was used for lymphocyte proliferation by culturing peripheral blood lymphocytes (PBL) with Coxsackievirus B5, Adenovirus 3, 4, and 7, and Poliovaccin.RESULTS & CONCLUSION:No significant differences were shown in the PBL proliferative percentage in response to Con-A mitogen and tested viruses (CVB5 and Adenovirus) between T1DM and healthy controls, but PBL proliferative percentage of patients showed a significant decline in response to Poliovaccine. HLA class II (-DR3, DR4, DQ2 and DQ3) antigens were significantly increased in T1DM patients and they played an important role in the etiology of the disease. Strong T-cell proliferation in response to the tested viral antigens were observed to be related to HLA-DR4 and HLA-DQ3 antigens, whereas the HLA-DR3 and HLA-DQ2 alleles were associated with week responsiveness to the same antigens. However, in children with new- onset diabetes, responses were decreased and this could be caused by trapping of virus- specific T- cells in the pancreas


Article
The possible Association of HLA Class II with Bladder Cancer in Iraqi Patients

Author: Shatha M.J.Al-Khateeb
Journal: Journal of the Faculty of Medicine مجلة كلية الطب ISSN: PISSN: 00419419 / EISSN: 24108057 Year: 2007 Volume: 49 Issue: 4 Pages: 434-437
Publisher: Baghdad University جامعة بغداد

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Background: - Genetic Factors have a major role in the development of bladder cancer. Objectives: - This study was carried out to shed a light on the possible association of HLA class II antigens and BC patients and to correlate this finding with the family history. Patients and Methodes :- Lymphocytotxicity assay had been used to assess HLA- typing of 65 BC patients and 50 healthy controls. Results:- comparison between BC patients and healthy controls showed several antigens deviations in their frequencies. HLA-DR1, HLA-DQ1 and HLA-DQ3 antigens were observed with increased frequencies in patients group with significant differences (P=0.000, 0.000 and 0.017 respectively). Moreover there was decrease frequency of HLA-DR7 in patients group (P=0.010). Stastical analysis showed non significant correlation of the specific HLA –Ags with family history. Conclusions: - This finding demonstrated that HLA-DR1, DR7, DQ1 and DQ3 might play a role in BC susceptibility. • Department of clinical biochemistry. Collage of Medicine Al-Mustansyriah University • Department of Microbiology. Collage of Medicine. Baghdad University • Ministry of Health.


Article
Is Chlamydia Pneumonia An Independent Risk Factor In Ischemic Heart Disease

Authors: Eman Sh. AL-Obeidy --- Eman Sh. AL-Obeidy
Journal: Iraqi Academic Scientific Journal المجلة العراقية للاختصاصات الطبية ISSN: 16088360 Year: 2010 Volume: 9 Issue: 2 Pages: 151-154
Publisher: The Iraqi Borad for Medical Specialization المجلس العراقي للاختصاصات الطبية

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ABSTRACT:BACKGROUND:Immune system may interplay between Chlamydia pneumoniae infection and ischemic heart disease (IHD).Major histocompatibility genes regulate innate and adaptive immunity..OBJECTIVE:This study was established to shed light on the possible association between ischemic heart disease (IHD) with Chlamydia pneumoniae infection and HLA antigens.PATIENTS AND METHODS:Microlymphocytotoxicity assay has been applied for HLA-typing of 150 blood samples of 100 IHD patients and 50 healthy normal controls, In addition enzyme linked immunosorbant assay (ELISA) used to detedct C pnuminiae IgA and IgG.RESULTS:An increased frequency of HLA-A*2, B*35 alleland HLA-A*2-B*35 haplotype was observed for patients group versus control group with P-value (0.0001, 0.05, and 0.001) respectively.CONCLUSION:This finding demonstrated that the HLA-B*35 positive haplotypes confer the C.pneumoniae-related risk for IHD. HLA-DR3,DR4 and DR7 might play a role in AIH susceptibility

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