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Article
Molecular Diagnosis of Coccidioides Posadasii from Aborted Placenta of Ewes by RT-PCR

Authors: Bushra Hamza Fares --- Ban A. Abdulmajeed --- Hameed Abdulhussein Mejbel
Journal: Kufa Journal For Veterinary Medical Sciences مجلة الكوفة للعلوم الطبية البيطرية ISSN: 20779798 Year: 2014 Volume: 5 Issue: 2 Pages: 24-31
Publisher: University of Kufa جامعة الكوفة

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Abstract

Diagnosis of diseases depends on histology using formaldehyde-fixed paraffin embedded tissue .The polymerase chain reaction (PCR)helps the pathologists to confirm the diagnosis of many disease including mycotic infections.Aborted placentas from 40 ewes were formalin fixed, paraffin embedded and sectioned. Tissue sections were stained with haematoxylin and eosine, and periodic acid Schiff (PAS) for histopathology examination. Fresh tissue parts were cultivated ,subjected to DNA extraction for the purpose of real-time PCR amplification.Histopathological examination of placental tissue revealed extensive necrotizing placentitis with the presence of spherule of Coccidioides Posadasiiin the necrotic area. Only eight samples gave positive growth for coccidioides Posadasii out of forty aborted placental samples.DNA amplification by real-time PCR revealed positive amplification for the eight samples which confirmed the detection of coccidiodes posadasii.


Article
Effect of Ovarian Stimulation Protocol on Embryo Quality in IVF-ICSI

Authors: Mufeda A. Jwad --- Ban A.Abdulmajeed --- Ula M. Al-Kawaz
Journal: Iraqi Journal of Embryos and Infertility Researches المجلة العراقية لبحوث الأجنة والعقم ISSN: eISSN: 26166984 / pISSN: 22180265 Year: 2018 Volume: 8 Issue: 1 Pages: 55-63
Publisher: Al-Nahrain University جامعة النهرين

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Abstract

Background:Defects in reproduction that may involve both the male & femalepartners result in the inability to procreate which is known as infertility. Theuse of assisted reproductive technologies (ART’s) has been increasing over thepast three decades. Many efforts have been done to compensate for unsatisfactoryresults in in-vitro fertilization (IVF) procedures, so that patients undergodifferent ovarian stimulation protocols to control retrieval of multiple oocytesin a single cycle. Although ovarian stimulation has an important role in ART’s,it may also have detrimental effects on oocyte quality, embryo quality, and IVFoutcomes.Objectives: To assess the effect of long agonist versus antagonist protocols onoocyte and embryo quality in IVF- Intracytoplasmic sperm injection (ICSI).Patients,Materials and Methods:This comparative study was done on the embryos of seventy one infertile femaleswho were undergoing Intracytoplasmic sperm injection (ICSI) treatmentregardless to the presence or absence of previous trials. These patients weredivided into two groups, 45 of them treated with agonist protocol and other 26patients treated with antagonist protocol. First oocyte retrieved was used forfurther assessment. Of the 71 oocytes, there were eight immature oocytes (3 atgerminal vesicle (GV) stage, 5 at metaphase I (MI) stage) and three abnormaloocytes, and these were excluded from the following ICSI treatment. Assessmentand comparison of oocyte maturity, fertilization rate, cleavage rate and resultingembryo quality were done.Results:The results of the present study showed significant difference (p<0.05)in oocyte number and maturity but no significant (p>0.05) difference betweenthe group treated with long agonist and those on antagonist protocol regardingfertilization rate, cleavage rate, and embryo quality.Conclusions:The type of protocol used in in-vitro fertilization has no detrimentaleffect on oocyte or embryo quality.


Article
Bronchial wash miR-21 as a potential biomarker for non-small cell lung cancer

Authors: Hussain Abady Aljebori --- Ban A. Abdulmajeed --- Adnan Aljubori
Journal: Iraq Joural of Hematology المجلة العراقية لامراض الدم ISSN: 20728069/25432702 Year: 2015 Volume: 4 Issue: 1 Pages: 39-60
Publisher: Al-Mustansyriah University الجامعة المستنصرية

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Abstract

Background: Lung cancer is one of the major health problems all over the world. Most ofcases are discovered at advanced stages because of late appearance of symptoms and the lackof efficient and effective methods for early diagnosis and screening of high risk groups. ThemicroRNA-21(miR-21) was stably present and reliably measurable in all samples of bronchialwash whether positive or negative (control) for lung cancer.Objectives: to evaluate the expressions of the miRNA-21 as a minimally invasive diagnosticbiomarker for non-small cell lung cancer (NSCLC).Results: Relative quantification of miR-21 gene showed overexpression in samples positivefor NSCLC (non-small cell lung cancer) and ROC study yielding 85% sensitivity and 98%specificity in distinguishing NSCLC patients from controls with p-value < 0.05.Conclusion: altered expressions of the miR-21 in samples of bronchial wash may provide apotential biomarker for detection of non-small cell lung cancer.


Article
Molecular Characterization of Dystrophin Gene in Iraqi Patients with Muscular Dystrophy
تحديد خصائص مورث الحثل العضلي في المرضى العراقيين المصابين بالضمور العضلي

Authors: Hula R. Abdulrasool Shareef حلا عبد الرسول --- Ban A. Abdulmajeed بان عباس عبد المجيد --- Areen T. Ramadhan Al-Hadithi ارين تحرير رمضان
Journal: Journal of the Faculty of Medicine مجلة كلية الطب ISSN: 00419419 / 24108057 Year: 2014 Volume: 56 Issue: 2 Pages: 181-185
Publisher: Baghdad University جامعة بغداد

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Abstract

Background: Dystrophinopathies are the commonest forms of muscular dystrophy and comprise clinically recognized forms, Duchenne Muscular Dystrophy (DMD), and Becker Muscular Dystrophy (BMD). Mutations in the dystrophin gene which consist of large gene deletions (65%), duplications (5%) and point mutations (30%) are responsible for reducing the amount of functional dystrophin protein in skeletal muscle fibers. This study concentrate mainly at the spectrum of deletions in the 'distal hot spot' region of the DMD/BMD gene in Iraqi DMD/BMD patients using multiplex PCR techniqueObjectives: The aim of this study was to investigate the rate, and distribution of deletions in 10 exons of Dystrophin gene in a group of Iraqi dystrophinopathy patients using the multiplex polymerase chain reaction (MPCR).Patients materials and methods: This is a case prospective study which include 27 clinically diagnosed DMD/BMD patients and six suspected carriers in Medical city /Baghdad . A written consent was obtained from each family for going the research as well as ethical committee approval. Forty six apparently healthy individual were included as a control group. Blood samples were collected in 5-6 ml EDTA tubes by venepuncture. The DNA was extracted by using the Wizard Genomic purification kit (Promega/USA), and the quantity was estimated by UV-spectrophotometer (Cecil CE7800) . Ten exons of the dystrophin gene were examined ( 19 ,45 ,46 ,47 ,48 ,49 ,50 ,51 ,52,53) using synthesized primers with complementary sequences and set in five different multiplex PCR groups. The products of PCR amplifications were subjected to electrophoresis and visualized by The UVCI140 & 200 series (advanced CCD gel imaging system from Major Science. It consists of a CCD camera, UV transilluminator UV- light system). All done in college of medicine /Baghdad university.Results: The rate (relative frequency) of subjects with any positive exonal deletion (among the10 selected and tested exons was significantly higher (85.2%) among patients compared to that among the suspected carriers (33.3%).The distribution of exonal deletions among patients compared to suspected carriers were statistically significant. The frequency of deletions detected in male patients (~82%) was higher than frequencies mentioned in the other studies of comparison. The control group show no deletion in all tested exons.Conclusions: Multiplex PCR technology was utilized to demonstrate the frequency of 10 exons deletions in a limited group of Iraqi DMD/BMD patients. The overall distribution of deletion mutations in the distal ‘hot spot’ region was higher than that of DMD/BMD cases investigated elsewhere. The study also serves as a good starting point for further investigations into the genetic aspects of the Iraqi DMD/BMD population.Keywords: Duchenne muscular dystrophy (DMD), Becker muscular dystrophy (BMD), Dystrophin gene, Multiplex PCR

خلفية البحث : تعتبر انواع الحثل العضلي التي تحدث بسبب اعتلال مورث الدستروفين من اشهر انواع الحثل العضلي وتتمثل بالحثل العضلي الدوشيني و الحثل العضلي البيكرز وتتضمن هذه الطفرات انواع متعددة منها طفرات الحذف الكبيرة (65%), طفرات التكرار (5%) و الطفرات النقطية (30%), وهي بمجموعها مسؤولة عن نقصان كمية بروتين الدستروفين الفعال في الالياف العضلية مما يؤدي الى تلف هذه الالياف و اصابتها بالاعتلال.الهدف : تهدف هذه الدراسه الى استكشاف معدل و انتشار طفرات الحذف في 10 اكسونات من مورث الدستروفين في مجموعه من المرضى العراقيين المصابين بالحثل العضلي الدوشيني/ البيكر وذلك باستخدام تقنيه تفاعل البلمرة المتسلسل المتعدد.المرضى و طرق العمل : تعتبر هذه الدراسه دراسه مستقبلية لدراسة الحالة و التي تضمنت 27 من المرضى المشخصين بالاصابة بالحثل العضلي الدوشيني/ البيكر اضافة الى 6 الحاملين المتوقعين للمورث المرضي. وتم اخذ موافقات كتابية من المرضى او عائلاتهم للموافقه على المشاركة في الدراسه بالاضافه الى موافقه اللجنه الاخلاقيه في كليه الطب. كما وتم ادخال 46 شخص معافين الى الدراسه لاستخدامهم كمجموعة قياسية. تم جمع نماذج الدم في انابيب (5-6) مل تحتوي مادة EDTA بواسطة السحب الوريدي. تم استخلاص الحمض النووي باستخدام استخدام عدة ( PROMEGA/USA) وتم فحص الكمية المستخلصة بواسطة جهاز فحص امتصاص الاشعة فوق البنفسجية (UV- absorbance).عشرة اكسونات في مورث الحثل تم فحصها (19و45و46و47و48و49و50و51و52و53) باستخدام الممهدات المصنعة بالمكملات التراتبية وتم وضعها في خمسة مجموعات لتقنية تفاعل البلمرة المتسلسل المتعددة. حيث ان كل مجموعه تضخم اثنين من الاكسونات. هذه الممهدات تكشف طفرات الحذف في هذه الاكسونات في المرضى والحاملين المحتملين لهذه الطفرات. تم اشراك مجموعة قياسية في الدراسة لغرض التحقق من صحة العمل. تستخدم المجموعات المضخمة بعد ذلك في جهاز الترحيل الكهربائي ويتم تصويرها بواسطة نظام الكاميرا فوق البنفسجية.النتائج : كان معدل الاشخاص المصابين بطفرات الحذف لجميع الاكسونات واضح في المرضى (85,2%) مقارنة بالحاملين المحتملين للطفرات (33,3%). ان انتشار هذه الطفرات كان واضح احصائيا بين المرضى مقارنة بالحاملين المحتملين للمرض. كانت نسبة تكرار طفرات الحذف بين الذكور اعلى (82%) من مثيلاتها في دراسات المقارنة ولم تظهر المجموعه القياسية اي طفرات في جميع الاكسونات المحددة في هذه الدراسة.الاستنتاج : تم استخدام تقنية تفاعل البلمرة المتسلسل المتعدد لاظهار معدل طفرات الحذف في عشرة اكسونات ضمن مجموعه محدده من المرضى العراقيين المصابين بالحثل العضلي الدوشيني/البيكر . ان الانتشار الكلي لطفرات الحذف في منطقة البقعة الساخنة القاصية كان اعلى من المعدلات المستحصلة في مرضى تم دراستهم في اماكن اخرى. تخدم هذه الدراسة كنقطة بداية في تحريات وراثية اضافية خاصة لمرضى الحثل العضلي الدوشيني/البيكر في العراق.مفتاح الكلمات : الحثل العضلي الدوشيني ( DMD) ، الحثل العضلي البيكر ( BMD) ، مورث الحثل العضلي ، تفاعل البلمرة المتسلسل المتعدد.

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