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Estimation of humural immune response on the rabbits that immunizing with Hydatid cyst antigens by using IHAT and ElISA.

Author: Ekhlas M. Edan* BSc, MSc اخلاص عيدان
Journal: Journal of the Faculty of Medicine مجلة كلية الطب ISSN: 00419419 / 24108057 Year: 2009 Volume: 51 Issue: 3 Pages: 332-335
Publisher: Baghdad University جامعة بغداد

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Abstract

Summary:Background: Hydatid disease also known as echinococcosis or hydatidosis , is caused by infection with larva (metacestoide) of tape worms of the genus Echinococcus. Materials and methods: Twelve Rabbits were immunized with three types of antigens (Hydatid cyst fluid antigen; Protoscoleces antigen and Germinal and Laminated layer antigen) according to a specific immunization program and we used indirect hemagglutination test( IHAT) and Enzyme Linked Immuno Sorbent Assay(ELISA) for detecting the anti-Echinococcus antigens in the serum of these animals. Results: The highest titter of antibodies were recorded in the Rabbit serum when use the first antigen (Hydatid cyst fluid) by using IHA method (1:64)after the end of immunization program while in ELISA method reached (1:128), The serum level for the rabbit serum that immunization with the second antigen(Protoscoleces) has reached (1:256) by using IHAT and (1:512)when use ELISA, The highest titter of third antigen (Germinal and Laminated layer)has reached (1:128) by using IHAT and (1:256) when we used ELISA method. Conclusion: The highest antibodies titer could be found when use the Protoscoleces antigen more than Cyst fluid and Germinal and Laminated layer antigen.Key words: Hydatid cyst, E.granulosus

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