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Antibiotic susceptibility patterns of Escherichia coli isolates from patients with significant bacteriuria

Author: Firas Srhan Abd Al- Mayahi
Journal: Al-Qadisiyah Journal of pure science(quarterly) مجلة القادسية للعلوم الصرفة (فصلية). ISSN: 19972490 Year: 2015 Volume: 1 Issue: 20 Pages: 29-49
Publisher: Al-Qadisiyah University جامعة القادسية

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Abstract

The aim of this study was to the isolation and identification of E. coli bacteria from patients with significant bacteriuria and Antibiotic susceptibility patterns. During the period from March 2011 to May 2012, a total of 2000 urine samples were collected from patients with suspected UTI. Isolates were identified by traditional biochemical tests, and then confirmed by VITEK 2 system. 455 (22.8%) samples were recognized as significant bacteriuria. The study documented that E. coli is the most important uropathogen causing UTI and recovered from 207 (45.5%) patients. However, of the patients with significant bacteriuria, a total of 143 (31.4%) positive urine cultures were implicated in nosocomial infections. Additionally, 312 (68.6%) positive urine cultures were implicated in community-acquired infections. According to demographic data, it was observed that the number of patients with significant bacteriuria was higher in females, 309 (67.9%) compared to males, 146 (32.1%). The mean age of these patients was 39.1 years range from 2-90 years (standard deviation, 18.1years). Majority of patients with significant bacteriuria (269, 59.1%) were in the age group 20-50 years. Sensitivity of all isolates was tested against 23 Antibiotics. Results showed all isolates of E. coli were resistant 100% to ampicillin but sensitive100% to imipenem, the antibiotics resistance rate among the tested E. coli isolates ranged from 92.7%-74.9%, 69.6%-31.4% and 48.3%-10.2%, present to cephalosporins, fluoroquinolone and aminoglycosides respectively.


Article
Incidence of Extended-Spectrum β-lactamases ESBLs producing Escherichia coli in patients with urinary tract infection

Author: Firas Srhan Abd Al- Mayahi
Journal: Al-Qadisiyah Journal of pure science(quarterly) مجلة القادسية للعلوم الصرفة (فصلية). ISSN: 19972490 Year: 2014 Volume: 19 Issue: 2 Pages: 37-50
Publisher: Al-Qadisiyah University جامعة القادسية

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Abstract

Two hundred seven isolates of Escherichia coli were isolated from urinesamples collected from patient suffering from UTI obtained from Al-DiwaniyaTeaching Hospital. During the period from March 2011 to May 2012, a total of 2000urine samples were collected from patients with suspected UTI. Identificationaccording to the traditional biochemical tests, then confirmed by VITEK 2 system.Detection of ESBLs by using initial screening and confirmatory test which carry outby disk approximation or double disk synergy test (DDS) and CHROMagar methods,showed that 186 (89.8%) / 207(100%) of E. coli were initially screened as positive forESBL. CHROMagar CTX technique was the most 166 (80.2%) ambient method thanthe disk approximation or (DDS) 0(0.0%) tests regarding confirmation of ESBLproduction. The results revealed that Screening and confirmatory tests considered themost accurate method for detection of ESBL- producing isolates, while the diskapproximation was not accurate method in detecting such enzymes. The results ofPCR assay showed that, 50 (100%) and 49 (98.0%) of the blaCTX-M positive isolatesharbored blaSHV and blaOXA genes, respectively. While, blaTEM, blaPER, blaVEB andblaGES genes were not detected in this investigation.

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