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Detection of mycobacterium tuberculosis in the saliva of patients having pulmonary tuberculosis

Author: Gassan Y. Hamed
Journal: Al-Rafidain Dental Journal مجلة الرافدين لطب الأسنان ISSN: 18121217 Year: 2011 Volume: 11 Issue: 3 Pages: 290-295
Publisher: Mosul University جامعة الموصل

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Abstract

Aims: The purpose of this study was to determine the efficacy of saliva as a sample for diagnosing pulmonary tuberculosis(T.B.) by looking for acid fast bacilli in a direct smear and comparing it with sputum and to determine whether the isolation of M. tuberculosis is from the lung or disseminated through blood. Material and Methods : The study sample consisted of 25 patients of both sexes. Age range was (17 - 65) years . Approximately 2 ml of unstimulated mixed saliva from each subject and parotid saliva were collected for direct smear for acid fast bacilli by Ziehl-Nelson acid fast stain. Five samples were inoculated on Lowenstein Jensen media and storenbrink media .Results: About 60% of unstimulated mixed saliva revealed positive acid fast bacilli, while all samples of parotid saliva showed negative acid fast bacilli. The five samples of saliva which were inoculated on Lowenstein Jensen media and stonebrink media showed positive culture. To our knowledge, we did not find any study performed on saliva as a sample for diagnosis of pulmonary tuberculosis to compare our finding with these studies, so the comparison was made between the sensitivity of saliva and the sensitivity of sputum . In this study, the sensitivity of direct smear of saliva for A.F.B was equal to 60% of the sensitivity of sputum. The sensitivity of direct smear of sputum for A.F.B. ranged from (2280%). There were no clinical manifestations like gummas; granulomas; ulcer; alveolar abscess and osteitis. CONCLUSION: This clinical and laboratory study revealed that M Tuberculosis which was present in the mixed saliva resulted from contact of oral tissue with infected sputum; Mixed saliva was less efficient than sputum in diagnosis of T.B. disease . Saliva can be inoculated on different media and that newly diagnosed patients with T.B disease don't have any clinical manifestations in the oral cavity


Article
Effectiveness Of Low Level Diode Laser (1064) And Acyclovir in Treatment Of Recurrent Herpes Labialis. (Comparative Clinical Study)

Authors: Gassan Y Hamed --- Ahmed S Khudhur --- Zaid A Alshamaa
Journal: Al-Rafidain Dental Journal مجلة الرافدين لطب الأسنان ISSN: 18121217 Year: 2017 Volume: 17 Issue: 1 Pages: 23-31
Publisher: Mosul University جامعة الموصل

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Abstract

Aims: To evaluate the clinical effectiveness of low level diode laser (1064) compared with the effect of Acyclovir (ACV) cream 5% in patients with recurrent herpes labialis (RHL). Materials and Methods: Thirty patients with active lesions (intact vesicle and rupture lesions) were included in this clinical trial. Fifteen patients were treated with low level diode laser (1064) while the other fifteen patients were treated with Acyclovir cream 5%. Results: Complete disappearance of prodromal symptoms ( itching, tingling or burning) and erythema were noticed in low level diode laser (1064) group after three days of treatment compared with ACV group. Conclusions: low level laser (1064) appeared to be more effective in healing time and pain relief than ACV cream 5%. Key words: Dental crowding; ClassII malocclusion; Dentoskeletal morphology.

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