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Article
-DIAGNOSIS OF CARRIER STATE OF FOOT AND MOUTH DISEASE VIRUS IN VACCINATED AND UNVACCINATED CATTLE BY RT-PCR

Author: Hazim T. Thwiny
Journal: Basrah Journal of Veterinary Research. مجلة البصرة للابحاث البيطرية ISSN: Print:18138497 E; 24108456 Year: 2016 Volume: 15 Issue: 2 Pages: 30-38
Publisher: Basrah University جامعة البصرة

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Abstract

The aim of present work were to investigate the carrier state to foot and mouth disease virus in vaccinated cattle (vaccinated carriers) and unvaccinated cattle (unvaccinated carriers). Reverse transcriptase polymerase chain reaction (RT-PCR) technique was employed to detect FMDV from esophageal and pharyngeal fluid samples collected from both vaccinated and unvaccinated cattle. Results have been shown that the persistence of FMDV is significantly higher in unvaccinated cattle in comparison to vaccinated animals. It have been concluded that high vaccination trials were play a role in the elimination of carrier status from cattle.

Keywords

RT-PCR --- Cattle --- Vaccinated animals


Article
Evaluation of the virucidal efficacy of commercial antiviral drugs against Lasota virus a surrogate for enveloped viruses

Authors: Amaal F. Ghanim --- Hazim T. Thwiny
Journal: Al-Qadisiyah Journal of Veterinary Medicine Sciences مجلة القادسية لعلوم الطب البيطري ISSN: 18185746 23134429 Year: 2018 Volume: 17 Issue: 2 Pages: 149-155
Publisher: Al-Qadisiyah University جامعة القادسية

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Abstract

Many antivirals are commonly used in Iraq’s poultry farms and there is controversy about effective of these commercial antiviral drugs. The aim of this study was tested individually for the effectiveness of these commercial antiviral drugs. Four kinds of commercial antiviral drugs including VIRUX®, TopAMD®, V8® and Licorice® was used in this study. Lasota virus was used as a surrogate for enveloped viruses. The following tests: Spot hemagglutination, Antigen Rapid NDV Ag Test Kit, Electron microscopy, qRTPCR and Egg inoculation in order to determine the effect of these antivirals on hemagglutinating activity, a viral protein, viral morphology, virus titer and viral infectivity respectively. All antivirals had no toxic effect on the chicken embryos. All these antivirals had no effect on haemagglutination activity except Licorice. No antivirals changed nucleoprotein antigenicity of Lasota virus. All antivirals had no effect on the morphology of the virus except Licorice destroyed the viral morphology and decreased in viral spikes. Three of the four antiviral reduced the viral titer while Licorice complete degradation of viral RNA and prevent detection it by qRTPCR. The allantoic fluid harvested from inoculated eggs with the treated Lasota virus with antivirals showed a remarkable decrease in viral infectivity as following: TopAMP about 20%, V8 40%, and Virux 60% while Licorice showed a complete reduction of viral infectivity (100%). In conclusion, the Licorice revealed the best antiviral activity.

Keywords


Article
Molecular Detection of Influenza A virus in Domestic Ducks and Geese in Basra Province, Southern Iraq

Authors: Firas T. Mansour --- Hazim T. Thwiny --- Harith A. Najim
Journal: JOURNAL OF THI-QAR SCIENCE مجلة علوم ذي قار ISSN: 19918690 Year: 2018 Volume: 6 Issue: 4 Pages: 125-129
Publisher: Thi-Qar University جامعة ذي قار

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Abstract

Avian influenza is one of the important viral diseases in poultry that can cause serious economic losses in many countries around the world, and occasionally infect human causing mild to severe illness. It is caused by avian influenza A viruseswhich have been isolated from avian species, particularly waterfowls that are considered the main reservoir for all influenza A virus subtypes. The aim of this study was to detect influenza virus from domestic ducks and geese in different geographical regions(Abu Al-Khasseb, Shatt Al-Arab, Zubair, and Al-Qurnah) of Basrah province, Southern Iraq. The study was conducted on 115 cloacal swabs collected from 60 ducks and 55 geese distributed in different regions of Basrah province. Samples were processed for RT-PCR to amplify matrix (M) gene that is conserved between all influenza A subtypes by using a set of universal primer. We demonstrated that influenza A viruses is prevalent in domestic waterfowls with a significantly higher percentage in ducks than geese; 42 samples (29 ducks and 13geese) were positive to viral M gene. In addition, the virus prevalence was significantly higher in the North of Basrah (Al-Qurnah region) than the other geographical regions. The overall findings of this study obtained a primary picture about the distribution of avian influenza viruses in our region, which is importantto prevent the spread of infection to poultry and eventually minimize the risk of acquiring infection to humans. According toour knowledge, this is the first study aimed to identify avian influenza viruses in waterfowls in Basrah province, Southern Iraq.


Article
Detection of Norovirus in stool samples by RT-PCR from under five years age children hospitalized for acute gastroenteritis in Basrah, Iraq
الكشف عن فيروس النورو في عينات البراز بواسطة تقنية تفاعل البلمرة المتسلسل العكسي في الاطفال الاقل من خمسة سنوات من العمر الراقدين في المستشفيات والذين يعانون من التهاب المعدة والامعاء الحاد في البصرة، العراق

Authors: Hazim T. Thwiny حازم طالب ثويني --- Hassan J. Hasony حسن جابر حسوني
Journal: JOURNAL OF THI-QAR SCIENCE مجلة علوم ذي قار ISSN: 19918690 Year: 2015 Volume: 5 Issue: 2 Pages: 47-53
Publisher: Thi-Qar University جامعة ذي قار

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Abstract

Human noroviruses are the leading cause of non-bacterial gastroenteritis in children worldwide. The objectives of this study are to determine the frequency and importance of norovirus in diarrheal illness among children below 5 years of age and to investigate the most affected age, seasonal distribution and the major clinical symptoms associated with norovirus infections; 400 stool samples (200 symptomatic cases and 200 asymptomatic healthy children) were investigated during the period from March 2011 to March 2012.Norovirus was detected in 8% of symptomatic children whereas all the control groups were norovirus negative. Norovirus was highly prevalent among children of 18-23 months of age (P=0.05). Noroviruses were continuously detected throughout the year except in May and October, but the principle peaks of detection were in November. The most common clinical symptoms beside diarrhea observed among norovirus infected children were vomiting (94%), fever (69%), dehydration (50%) and abdominal pain found in 31%.

تعتبر فيروسات النورو الخاصة بالانسان مسببا رئيسيا لالتهاب المعدة والامعاء الحاد الغير بكتيرية على مستوى العالم. اهداف هذه الدراسة تحديد اهمية وتكرار فيروس النورو بين الاطفال المصابين بحالات اسهال وتقل اعمارهم عن خمسة سنوات, لتحديد العمر الاكثر تعرضا للمرض, الانتشار الموسمي للمرض والعلامات الرئيسية المصاحبة للاصابة. تم فحص 400 عينة من البراز (200 من الحالات المرضية و 200 من الحالات الغير مرضية) خلال الفترة من اذار 2011 الى اذار 2012. كانت نسبة وجود الفيروس هي 8% في الاطفال الذين تظهر عليهم علامات سريرية, بينما في مجموعة السيطرة لم يتم الكشف عن الفيروس فيها. الفيروس كان اكثر شيوعا في الاطفال بعمر 18-23 شهر (P=0.05). اما بالنسبة للانتشار الموسمي للمرض, فقد تم الكشف عن الفيروس في كل اشهر السنة ما عدا شهري ايار وتشرين الاول وان الاصابات الاكثر كانت في شهر تشرين الثاني. اهم الاعراض السريرية بجانب الاسهال بالاطفال المصابين بالفيروس كان التقئ بنسبة 94%, الحمى بنسبة 69%, الجفاف بنسبة 50%, والم في البطن بنسبة 13%.

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