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Article
Estimation of some T-helper 2 cytokines (IL-4 and IL-10) in HBV Infected Patients and Individuals Vaccinated with Recombinant HB Vaccine
تقدير بعض سايتوكاينات النوع الاول للخلايا المساعدة التائية (IL-8 and IL-4) في المرضى المصابين بالتهاب الكبد الفايروسي نمط ب والافراد الملقحين بلقاح التهاب الكبد نوع ب المحور

Author: Hiwa Abdul -Rahman Ahmed
Journal: Tikrit Journal of Pure Science مجلة تكريت للعلوم الصرفة ISSN: 18131662 Year: 2011 Volume: 16 Issue: 1 Pages: 5-9
Publisher: Tikrit University جامعة تكريت

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Abstract

infection, interleukin-4 (IL-4) and IL-10 are considered examples on T-helper 2 (Th2) cytokines which required for host antiviral immune response and involved in humeral immunity against HBV infection. This study was designed to estimation Th2 cytokines (IL-4 and IL-10) in HBV infected patients and individuals vaccinated with recombinant HB vaccine. Study groups were classified into patient group 35(15 acute (AH) and 20 chronic (CH)), 35 vaccinated group (20 responder (RD) and 15 Non-responder (NRD)) and 18 healthy control (HC) during May to November 2007. Blood samples were taken from patients and hospitals staffs to detection HBsAg, Anti-HBc Ab(IgM), Anti-HBs Ab, IL-4 level and IL-10 level in serum by enzyme linked immunosorbent assay (ELISA) test.The concentration of IL-4 and IL-10 levels in the AH group differed significantly compared with HC and CH patients (p<0.01) by F-test. LSD-analysis for IL-4 revealed same result only between HC and CH (P<0.008). Comparison between CH and both HC and AH, respectively revealed significant differences in IL-10 level (P<0.008) and (P<0.03) by LSD-test. F-test for IL-4 revealed non significant result (P>0.05) among RD, NRD and HN control in ≥30 and <30 years old, respectively, but inverse result was observed in IL-10 levels (p<0.05). LSD-test revealed (P>0.05) between all study group regarding IL-4, however the level of IL-10 were (P<0.014) and (P<0.01) in RD and NR groups among ≥30 and <30 years old, respectively. Indeed (P<0.03) between RD and HN groups in age <30 years. Conclusion: In this study significant elevation of IL-4 and IL-10 levels was observed in AH patients compared with CH patients and healthy control. Same result was seen in IL-10 among RD and NRD in ≥30 and <30 years old, as well as between RD and HN groups in age <30 years

للاستجابة المناعية الخلوية دور مهم في تنخر الكبد المرافق لمرض التهاب الكبد الفايروسي. يعتبر IL-4وIL-10 من الامثلة على السايكوتينات المنتجة من خلايا Th -2 و الذي يحتاجها المضيف كاستجابة مناعية ضد الفايروسات بالاضافة لدورهم في المناعة الخلطية ضد مرض التهاب الكبد الفايروسي. صممت هده التجربة لحساب سايتوكينات خلايا Th-2 خاصة IL-4وIL-10 للمرضى المصابين بالتهاب الكبد الفايروسي نوع ب والاشخاص الملقحين بلقاح HB.مجاميع المرضى صنفت الى مجموعة المرضى35 مريض (15 حاد( (ACو20 مزمن(Ch)) 35 هم مجموعة الملقحين (20 المستجيبين للقاح( (RDو15 غير المستجيبين للقاح (NRD)) بالاضافة الى 18 شخص كمجموعة سيطرة خلال الفترة من ايار الى تشرين الثاني 2007.اخدت نمادج الدم من المرضى والاصحاء من عدة مستشفيات منها مستشفى نانكلي واربيل ورزكاري التعليمي لتشخيص كل مما يلي HbsAgو Anti-HBc Ab(IgM)و Anti-HBs Abوكذلك تقدير مستويات IL-4و IL-10 في المصل بواسطة فحص الاليزا.مستويات كل من IL-4 و IL-10 للمرضى المصابين بالتهاب الكبد الفايروسي الحاد اختلفت معنويا بالمقارنة مع مرضى التهاب الكبد المزمن ومجموعة السيطرة (p<0.01) عند التحليل الاحصائي بالاختبار F, بينما الاختبار الاحصائي LSD لل IL-4ايضا اعطت نفس النتائج لكن بين HC و CH عند مستوى .(P<0.008) عند المقارنة بين CH و HCو AHعلى التوالي هناك فروقا معنويا في مستوى IL-10 (P<0.008) و (P>0.05)وذلك باستخدام التحليل الاحصائي LSD.اختبار F للIL-4 اعطت فرقا معنويا (p<0.05) عند مقارنة RDو NRDوالسيطرة الغير الملقحين في المجموعة العمرية 30 ≤ و < 30 سنة بالتتابع ولكن النتائج كانت مغايرة في مستوى IL-10 }(p<0.05). اختبار LSD للIL-4 لم تعطي فرقا معنويا (p<0.05) بين كل مجاميع الدراسة لكن كانت الفروق معنوية (P<0.014)و(P<0.01) عند مقارنة RDو NRDوالسيطرة الغير الملقحين في المجموعة العمرية 30 ≤ و < 30 سنة بالتتابع اضافة لدلك كانت الفروق معنوية(P<0.03) بين مجاميع RD و HN في المجموعة العمرية30 < سنة. الاستنتاجات : توصلنا في هذه الدراسة ان هناك ارتفاعا معنويا في مستوى IL-4وIL-10 في مصول المرضى المصابين بالتهاب الكبد الفايروسي الحاد بامقارنة مع المرضى المصابين بالتهاب الكبد الفايروسي المزمن ومجموعة السيطرة. نفس النتائج سجلت بالنسبة لمستوى مستوى IL-10 في مجاميع RDو NRD في المجموعة العمرية 30 ≤ و < 30 سنة بالتتابع كذلك بين مجاميع RD و HN في المجموعة العمرية30 < سنة.

Keywords

IL-4 --- IL-10 --- HBV --- recombinant HB vaccine.


Article
Estimation of Immunoglobulin (IgG and IgM) in Hepatitis B Virus Infected Patients and Individuals Vaccinated with Recombinant HB Vaccine

Authors: Hiwa Abdul -Rahman Ahmed --- Rukia Muhammad Al-Barzinji
Journal: Iraqi Academic Scientific Journal المجلة العراقية للاختصاصات الطبية ISSN: 16088360 Year: 2010 Volume: 9 Issue: 3 Pages: 257-261
Publisher: The Iraqi Borad for Medical Specialization المجلس العراقي للاختصاصات الطبية

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Abstract

ABSTRACT:BACKGROUND:Hepatitis B virus (HBV) infection is a major public health problem. Successful clearance of the virus depends on a complex interaction between the virus and the host immune response.OBJECTIVE:The purpose of this study was to assessment of some immunoglobulins (IgG and IgM) in hepatitis patients and vaccinated groups compared with healthy control.METHODS:Study groups were classified into patient group 35 (15 acute (AC) and 20 chronic (CH)) and 35 vaccinated group (20 responder (RD) and 15 Non-responder (NRD)) and 18 healthy non vaccinated control group (HN). This study extended through a period from May to November 2007. Blood samples were taken from patients and hospitals staffs in Nanakaly, Erbil and Rizgary Teaching Hospital/ Erbil/ Iraq to detection of IgG and IgM level in serum by immunoturbidimetric test.RESULTS:The mean serum IgG level significantly increased in CH patients (p<0.05) compared to AH and HN control groups, in contrast IgM level increased high significant (p<0.001) in AH than CH and HN groups. No significantly differences in serum immunoglobulins (IgG and IgM) between vaccinated individuals and HN group (p>0.05).CONCLUSION:Present study indicate that serum IgG level was higher in CH patients whereas IgM level increased in AH. No significant differences in their levels were observed between vaccinated individuals and HN group.

Keywords

hepatitis --- immunoglobulin --- chronic --- acute --- vaccine


Article
Estimation of GM-CSF in Hepatitis B Virus Infected Patients and Individuals Vaccinated With Recombinant HB Vaccine

Authors: Hiwa Abdul -Rahman Ahmed --- Rukia Muhammad. Al-Barzinji
Journal: Iraqi Academic Scientific Journal المجلة العراقية للاختصاصات الطبية ISSN: 16088360 Year: 2010 Volume: 9 Issue: 2 Pages: 210-213
Publisher: The Iraqi Borad for Medical Specialization المجلس العراقي للاختصاصات الطبية

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Abstract

ABSTRACT:BACKGROUND:Hepatitis B virus (HBV) infection is a major public health problem. Successful clearance of the virus depends on a complex interaction between the virus and the host immune response.OBJECTIVE:This study was designed to estimation granulocyte macrophage-colony stimulating factor (GM-CSF) in HBV infected patients and individuals vaccinated with recombinant HB vaccine.METHODS:Study groups were classified into patient group 31(15 acute (AH) and 16 chronic (CH)), 33 vaccinated group (20 responder (RD) and 13 Non-responder (NRD)) and 16 healthy control (HC) during May to November 2007. Blood samples were taken from patients and hospitals staffs to detection hepatitis B surface antigen(HBsAg), anti-hepatitis B core antibody(IgM)(Anti-HBc Ab(IgM), anti hepatitis B surface antibody) (Anti-HBs Ab), GM-CSF level in serum by enzyme linked immunosorbent assay (ELISA) test.RESULTS:A highly significant elevation (p<0.001) of GM-CSF level was reported in AH patients compared to the CH patients and HN controls, according to F-test, while in CH patients the elevation was not significant as compared to HN control (p>0.05) according to LSD- test. The level of GM-CSF also significantly higher (p<0.05) in RD at age ≥30 years than NRD and HN groups by F-test. Also a significant differences was recorded when compared between HN versus RD and RD versus NRD (P<0.027) and (P<0.023) respectively.CONCLUSION:In this study highly significant elevation of GM-CSF levels was observed in AH patients compared with CH patients and healthy control. GM-CSF level also significantly higher in RD at age ≥30 years than NRD and HN group


Article
Estimation of T-helper 1 Cytokines (IFN-y and TL-2) in HBV Infected patients and Individuals vaccinated with Recombinant HB Vaccine
تقدير السيتوكينات T-helper 1 (IFN-y و TL-2) في المرضى المصابين بفيروس HBV والأفراد الذين تم تطعيمهم بلقاح HB المؤتلف

Authors: Hiwa Abdul -Rahman Ahmed --- Rukia M. Al-Barzinji
Journal: Zanco Journal of Medical Sciences مجلة زانكو للعلوم الطبية ISSN: 19955588/19955596 Year: 2009 Volume: 13 Issue: 2 Pages: 50-56
Publisher: Hawler Medical Univeristy جامعة هولير الطبية

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Abstract

Background and Objectives: The specific cellular immune responses play a main role in the hepatic necrosis that occurs with hepatitis B virus (HBV) infection. Interferon-g (IFN-g) and interleukin-2 (IL-2) are considered examples on T helper 1(Th1) cytokines which required for host antiviral immune response and involved in cell-mediate immunity against HBV infection. This study was designed to estimation T-helper 1 cytokines (IFN-g and IL-2) in HBV infected patients and individuals vaccinated with recombinant HB vaccine.Methods: The study groups were classified into patient group 35 (15 acute hepatitis (AH) and 20 chronic hepatitis(CH)) and 35 vaccinated group (20 responder (RD) and 15 Nonresponder (NRD)) and 18 control group, during May to November 2007. Blood samples were taken from patients and hospitals staffs in Nanakaly, Erbil and Rizgary Teaching Hospital to detect hepatitis B surface antigen (HBsAg), anti hepatitis core antibody IgM (Anti- HBc Ab(IgM)), Anti-HBs Ab, IFN-g level and IL-2 level in serum by enzyme linked immunosorbent assay (ELISA).Results: The concentration of IFN-g and IL-2 levels in the AH group differed significantly compared with healthy control and CH patients (p<0.01) by F-test. LSD-analysis for IFN-g also revealed same result while IL-2 level significantly increased in healthy control only. Ftest for IFN-g revealed (p<0.05) among RD group, NRD group and healthy non vaccinated (HN) control in ≥30 and <30 years old respectively but inverse result was observed in IL-2 levels (p>0.05).

Keywords

IFN-g --- IL-2 --- HBV --- Recombinant HB vaccine

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