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Article
Screening for Genetic Polymorphisms of Glutathione- S-Transferase Genes and Risk Factors Among Breast Cancer Patients in Iraq
التحري عن التباين الوراثي لجين GSI وخطر الاصابة بسرطان الثدي عند المرضى العراقيين

Authors: Ali Al-Zaag علي الرحمن الزعاك --- Ismail H. Aziz اسماعيل حسين عزيز
Journal: Iraqi Journal of Cancer and Medical Genetics المجلة العراقية للسرطان والوراثة الطبية ISSN: 20786123 Year: 2010 Volume: 3 Issue: 2 Pages: 12-17
Publisher: Al-Mustansyriah University الجامعة المستنصرية

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Abstract

The study included 40 Iraqi women diagnosed as breast cancer patients who attended the Nuclear MedicineHospital in Baghdad and 60 apparently healthy women as control .Genomic DNA extraction was performed usingproteinase K / SDS method. Multiplex PCR techniques were followed to amplify the genes using specific primers.for detection of gstm1, gstt1 and albumin gene as a control. Genetic analysis showed that the percentage of deletionsin breast cancer patients was 75% (gstm1 27.5%, null genotype 32.5 % and 15% for gstt1) versus 10 % were in thecontrol samples (5 % gstt1, 3.33% gstm1 and 1.67% null genotype). Data were subjected to statistical analysis usingChi square ( X2 test ) to evaluate the association between etiological risk factors and having a risk for breast cancer .A significant association was considered at level ( P < 0.05 ) . Blood group O and A showed an interesting associationwith breast cancer risk. The percentage of deletion was 90.9% and 86.6% in O and A blood group, respectively. Ageand family history had no significant risk association with breast cancer (P > 0.05) in this study.

ألخلاصة:رطان الثدي

Keywords

GST genes --- Breast Cancer --- Iraq.


Article
Detection of LT and ST Toxin genes for E.coli isolated from UTI
الكشف عن جينات الذيفان Lt و St لبكتريا الاكولاي والمعزولة من التهاب المجاري البولية

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Abstract

This study design ,study the importance of recurrence Urinary Tract infection caused by enterotoxigenic Escherichia coli (ETEC) via detect LT and ST toxin genes in E.coli samples isolated from patients with UTI. A total of 60 urinary samples were collected from patients with UTI especially from children Infected in the Central Child Hospital and Al-Yarmouk Teaching Hospital in Baghdad for the period 27/12/2011 to 7/7/2012.isolation of E. coli was performed according to the standard laboratory methods ,Agglutination test was done in the Central Health Laboratory and API 20E system was done in the Children Teaching Hospital. DNA was extracted from samples by High-Speed Plasmid Mini Kit DNA and pure yield plasmid miniprep system were used as a template for PCR reaction. Two sets of primers were used to simultaneously detect the genes encoding LT and ST by multiplex PCR assay. Out of 60 samples ,15/60 negative (25%),45/60 positive (75% ), and infection in males was 6/45 (13.3%) While in females 25/45(56%). The percentage of LT gene 9/45 (20%), while none of samples were carrying ST gene. The results indicate that ETEC strains , with a relatively conserved genetic pool, are capable of causing urinary tract infection. Due to the importance of these strains in public health, it is suggested that the conventional methods used for their detection less accurate than molecular method Because of the great similarity with cholera toxin .

إن الهدف هو دراسة أسباب الإصابة المتكررة بالتهاب المسالك البولية نتيجة الإصابة ببكتريا enterotoxigenic Escherichia coli (ETEC) عن طريق الكشف عن جينات الذيفانات ( LT ,ST ) والمحمولة على البلازميدات والموجودة في بكتريا Escherichia coli والمعزولة من المرضى لمصابين بالالتهاب المسالك البولية UTI)). حيث تم جمع 60 نموذج من الإدرار من المرضى المصابين بالالتهاب المجاري البولية وبالأخص الأطفال الراقدين في مستشفى الطفل المركزي واليرموك التعليمي في بغداد وفي الفترة 2011/12/27 إلى 2012/7/7 , و أثبتت إن العزلة كانت تعود إلى بكتريا الــ E.coli بالاختبارات االمختبرية التقليدية والاختبارات المصلية التي أجريت في مختبر الصحة المركزي , واختبارات API 20E التي أجريت في مستشفى الطفل المركزي , إذ كانت 1560 عزلة سالبة للفحص أي بنسبة 25% و 2560 عزلة موجبة للفحص أي بنسبة 75%, وان نسبة إصابة الذكور 645 أي بنسبة 13,3% بينما الإناث كانت 2545 أي بنسبة 56%. استخلص ألــ DNA البلازميدي من عزلات E.coli باستخدام High-Speed Plasmid Mini Kit حيث استخدم بعدها كقالب في تفاعلات PCR إذ تم استخدام نوعين من البادئات وإدخالها في تفاعل PCR واحد .كانت نسبة جينات ألـ lt ( 945) 20% في حين لم يتم الحصول على جينات ألـ st .وبذلك أثبتت النتائج أن سلالة ETEC تحمل جينات الضراوة مما يجعلها قادرة على إحداث الإصابة بــ UTI(لذا تعتبر مهمة في مجال الصحة العامة ) , إن الاختبارات المصلية و المختبرية التقليدية لم تعد كافية للكشف بالإضافة إلى إنها اقل دقة من الاختبارات الجزيئية في التفريق بين أنواع البكتريا وأسباب إحداث الإصابة .

Keywords

ENTEROTOXIN --- E. COLI --- LT --- ST --- ENTEROTOXIN --- E. COLI --- LT --- ST


Article
Identification Pseudomonas aeruginosa by 16s rRNA gene for Differentiation from Other Pseudomonas Species that isolated from Patients and environment
تشخيص بكتريا Pseudomonas aeruginosa بـجين 16s rRNAلتفريقها عن بقية انواع جنسها المعزولة من المرضى والبيئة

Authors: Abeer A. Marhoon محمد ابراهيم الطائي --- Ismail H.Aziz اسماعيل حسين عزيز --- Mohammed E. Altaai عبير علي مرهون
Journal: Baghdad Science Journal مجلة بغداد للعلوم ISSN: 20788665 24117986 Year: 2014 Volume: 11 Issue: 2 عدد خاص بالمؤتمر النسوي الثاني Pages: 1028-1034
Publisher: Baghdad University جامعة بغداد

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Abstract

Pseudomonas aeruginosa is a common and major opportunistic human pathogen, its causes many and dangersinfectious diseases due to death in some timesex: cystic fibrosis , wounds inflammation , burns inflammation , urinary tract infection , other many infections otitis external , Endocarditis , nosocomial infection and also causes other blood infections (Bacteremia). thereforebecomes founding fast and exact identification of P. aeruginosafrom samples culture very important.However, identification of this species may be problematic due to the marked phenotypic variabilitydemonstrated by samples isolates and the presence of other closely related species. To facilitate species identification, we used 16S ribosomal DNA(rRNA) sequence data toidentify genus- and species-specific 16S rRNA signature sequences,its account a stable part of the geneticcode. Based on these sequences we designed simple, rapid, and accurate PCR assays that allow the differentiation of P. aeruginosa from Pseudomonas species and other pathogen genus ,also this test considered as the most specific than the other diagnostic tests like API (20) which give 70% while the 16SrRNA test give more than 90 %.

تعد بكتريا الـPseudomonas aeruginosa الاكثر شيوعا وخطورة من بين الممرضات الانتهازية التي تصيب الانسان ،اذ تسبب العديد من الامراض المعدية الخطرة التي تؤدي الى الموت في بعض الاحيان مثال ذلك التكيس الليفي ، التهاب الجروح، التهاب الحروق ، التهاب المجاري البولية ، والعديد من الاصابات الاخرى . لذلك اصبح من المهم جدا ايجاد طريقة سريعة ودقيقة لتشخيص بكتريا الـ Pseudomonas aeruginosa من العينات المرضية المزروعة على الاوساط الزرعية وذلك لان تشخيص هذا النوع يواجه مشكلة التغاير في النمط المظهري الملحوظ في العينات المعزولة التي تحتوي على العديد من الانواع المتقاربة جدا فيما بينها وابسط طريقة للتفريق بين هذه الانواع هي استخدام الـ 16SrRNA الذي يعطي تشخيصا للجنس والنوع اذ يعد الشفرة الوراثية الثابتة للنوع وبذلك . هدفت هذه الدراسه الى ايجاد تشخيص سريع وبسيط ودقيق بتقنيةPCR يهيأ لنا تشخيص بكتريا الـPseudomonas aeruginosaوتفريقها عن باقي انواع جنسها واجناس مرضيه اخرى وكذلك يعتبر هذا الاختبار الأدق من بين الاختبارات التشخيصية الاخرى مثل الـAPI (20) حيث يعطي نسبة 70% في حين يعطي التشخيص بالـ16SrDNA اكثر من %90.


Article
6.DETECTION OF ExoT GENE IN LOCAL ISOLATES OF PSEUDOMONAS AUROGINOSA IN A SAMPLE OF BURN INFECTION

Authors: Rana A. Hanoon رنا عادل حسون --- , Ibtisam G. Auda ابراهيم غبان عايد --- Ismail H. Aziz اسماعيل حسين عزيز
Journal: IRAQI JOURNAL OF MEDICAL SCIENCES المجلة العراقية للعلوم الطبية ISSN: P16816579,E22244719 Year: 2017 Volume: 15 Issue: 4 Pages: 358-363
Publisher: Al-Nahrain University جامعة النهرين

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Abstract

Background: Pseudomonas aeruginosa (P. aeruginosa) is an opportunistic microorganism that requires damaged mucus membranes and epithelial tissues to cause acute infections. It had been stated that P. aeruginosa alters mammalian cytokinesis in a type III secretion system and exotoxin T (ExoT)-related way.Objective: To identify exoT gene local isolates of P. aeruginosa isolated from burn infections.Methods: Forty bacterial isolates of P. aeruginosa (isolated from burn infection) were identified by standard laboratory methods and polymerase chain reaction (PCR) technique was applied for the detection of the gene encoding for ExoT. Results: The results showed that PCR amplification of exo T gene occurred in 24 (60 %) isolates out of the enrolled 40 isolates of P. aeruginosa while 16 (40 %) of the isolates showed negative amplification reactions.Conclusion: It appeared that exoT can be a significant virulence factor expressed by 60 % of P. aeruginosa isolates as indicated by positive PCR-amplification results.Keywords: Burn infections, Exotoxin T, type III secretion system, PCR, P. aeruginosa Citation: Hanoon RA, Auda IG, Aziz IH. Detection of ExoT gene in local isolates of Pseudomonas auroginosa in a sample of burn infection. Iraqi JMS. 2017; Vol. 15(4): 358-363. doi: 10.22578/IJMS.15.4.6


Article
Molecular Detection of fnbA, fnbB and nuc Genes and Phenotypic Detection of some Virulence Factors in Local Staphylococcus aureus Isolates.

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Abstract

In this study, Staphylococcus aureus (S. aureus) isolates (32) were collected from different hospitals in Baghdad and were diagnosed by conventional methods. Antimicrobial susceptibility test was performed by disc diffusion method for ten antibiotics. Hemolytic production activity and Deoxy ribonuclease (DNase) production ability were detected. The biofilm production ability was detected by microtiter plate method. The diagnosis was confirmed using PCR to detect the thermostable nuclease gene (nuc). Then, PCR technique was used to detect the fibronectin binding protein A and fibronectin binding protein B genes (fnbA and fnbB respectively).The results showed a high prevalence (78%) of Methicillin-resistant Staphylococcus aureus (MRSA) isolates, Imipenem and vancomycin were the most affective antibacterial agents tested. All isolates produced DNase enzyme . On blood agar plate, 65.6% of isolates produced β-hemolysis zones while (34.4%) of isolates did not produce any hemolytic zone. The results of biofilm production assay showed that 41% of isolates gave a weak positive results. All isolates (100%) that had been previously diagnosed as S. aureus by routinely used methods harbored the nuc gene. fnbA and fnbB were found in (100%) and (25%) of isolates respectively.Conclusion: Molecular detection of nuc gene by PCR has high specificity for identifying S. aureus with a low cost which requires less time as compared with biochemical methods. fnbA and fnbB genes are important virulence factors of S. aureus. fnbA gene is the highest prevalence of toxinogenic and can be used as a genetic marker for diagnosis of local S. aureus isolates while fnbB gene does not play an important role in infection of S. aureus.

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