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Molecular Study of Sortase Enzyme and Characterization of Some Virulence Factors in Streptococcus pyogenes

Authors: Mohammed Sabri A. Razak --- Rafah Fakri Al-Jebori
Journal: Medical Journal of Babylon مجلة بابل الطبية ISSN: 1812156X 23126760 Year: 2012 Volume: 9 Issue: 1 Pages: 74-83
Publisher: Babylon University جامعة بابل

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Abstract

This study included 90 throat swabs collected from patients suffering from acute pharyngitis who attending to AL-Hilla General Teaching Hospital (ENT unit) during the period from October 2010 to January 2011. The age of the patients ranged from (3 to 63) years. Only six isolates of Streptococcus pyogenes were obtained. It was observed that only four isolates had the fibrinolytic activity through the production of streptokinase enzyme, while two isolates failed to lyse fibrin, also the present study showed that streptokinase activity became apparent after 3 hours of incubation and clot began to decrease after 10-16 hours and disappeared completely after 24 hours of incubation. Hyaluronidase activity was investigated, it was found that 50% (3) of GAS isolates give a positive result via the formation of a hallo around the colony and this is an indicator of hyaluronidase activity. Specific primers for PCR techniques were used for the detection of sortase enzymes (A, B and C). Sortase A was found existed in all GAS strains and this enzyme was designated as housekeeping enzyme. Sortase B and C was also detected ,it was found that only two isolates were positive for both sortase B and C genes. However, only two isolates were found to have all these three enzymes whereas the others contained only the housekeeping one.

من مجموع 90 مسحة بلعوميه قد تم الحصول عليها من مرضى تتراوح أعمارهم (3-63) سنة يعانون من التهاب البلعوم من المراجعين إلى مستشفى الحلة الجراحي (وحدة ENT) للفترة من اكتوبر2010 إلى آذار 2011 ,تم عزل ستة عزلات فقط من بكتريا المسبحيات القيحية (Streptococcus pyogenes) . ظهر أن أربعة عزلات من بكتريا المسبحيات القيحية لها القابلية على تحليل الفايبرين من خلال أنتاجها لانزيم Streptokinase. كما أظهرت بعض العزلات أن فعالية أنزيم Streptokinase تظهر واضحة بعد ثلاثة ساعات من الحضن وأن الخثرة (clot) تبدأ بالتقلص بعد 10-16 ساعة وتختفي تماما بعد 24 ساعة. تم التحري عن فعالية أنزيمHyaluronidase وقد وجد أن 50% من العزلات أعطت نتائج موجبة من خلال تكوين منطقة شفافة حول المستعمرة وهذا يعتبر مؤشر على وجود فعالية لهذا الأنزيم.تم استخدام بادئات نوعية (primers) في تقنية تفاعل البلمرة المتسلسل (PCR) للتحري عن الجينات الخاصة بأنزيمات Sortase (A, B C) وقد أظهرت النتائج بامتلاك كافة العزلات لجين Sortase A في حين عزلتين فقط أظهرتا احتواءها لجين Sortase B وSortase C .على أية حال فأن نتائج PCR أظهرت بأن عزلتين فقط تمتلك الجينات للأنواع الثلاثة من أنزيم Sortase.

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Article
•Immunological Study On Breast Cancer In Hilla Province

Authors: Ifad Kerim Al-shibly --- Mohammed Abod Muhsin --- Mohammed Sabri A.Razak
Journal: Karbala Journal of Medicine مجلة كربلاء الطبية ISSN: 19905483 Year: 2011 Volume: 4 no 2 Issue: 10 Pages: 1140-1145
Publisher: Kerbala University جامعة كربلاء

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Abstract

background: Cancer is one of the leading causes of death in the general population. Breast cancer is the top. In Iraq, where the population was exposed to high levels of depleted uranium following the first and second Gulf Wars, breast cancer became the most common tumor type in females.1Interleukin-10 (IL-10) and Interleukin 12 (IL-12) are the main immunological interplayers against cancer, and their impairment can be observed in many cancers, including breast cancer. Over the last ten years, there has been a three-fold increase in the incidence of breast cancer, with most of this increase being attributed to a particularly aggressive type of the cancer.Objectives: The high incidence rate of breast cancer in the last few years in Iraq inspired us to create an investigation about the immunological bases of breast cancer in a series of local Iraqi women to see whether any differences in this immunological profile could be found in a population exposed to depleted uranium.Methods: A Case-control study was designed and applied. A total of 40 subjects were chosen for the immunological study. Blood samples were obtained at Hilla General Teaching Hospital, Breast Cancer Centre (Pathology Department). The ELISA method was used for the interleukins and β2-microglobulin detection.Results: In this study, the association between the levels of the IL-10 , IL-12 and β2microglobuline and breast cancer was evaluated. The data showed significant increase in these cytokines levels among breast cancer patients and healthy controls.Conclusions: The conclusions that can be extrapolated by this study are that, there was a significant increase in the levels of the IL-10 , IL-12 and β2-microglobuline among breast cancer patients and healthy controls.

Keywords

Breast cancer --- interleukins --- Iraq

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