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Tissue Nucleic Acids Contents and the Activities of Their Turnover lnzymes: (RNase and DNase) in Patients with Benign and Malignant Ilterine Tumors

Authors: Shatha A. Al-Shammaree --- Ilathana R- Hasan
Journal: Medical Journal of Babylon مجلة بابل الطبية ISSN: 1812156X 23126760 Year: 2005 Volume: 2 Issue: 4 Pages: 415-423
Publisher: Babylon University جامعة بابل

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Abstract

DNA and RNA wefe isolaled from tissue hornogenates of pathological contlol, benign andmalignant utefine tumo$ using phe ol exiraction method. The purification of isolated DNA and RNA werecaried ou! using spin-column method.-fhe DNA and RNA contents (myml) were found to be highef in the malignant tunor trssueshomogenates than those of coresponding pathological contfol and benign utefine tumor trssueThe acid and alkaline bonuclease (RNase) activities and acid deoxyribonuclease (DNase) aclivilywere evaluaied in sera ofconnol. benign and malignant utefine tumors patients. I'lighly significant elevation(P<0.001) ofthese enzymes activities werc found in cancer gr oup in compadson wirh that of other g oups.No differ€nces in the thrce enzymes aclivities were found among the subgroups (pre-menopausal,menopausal. post rnenopausal patients) ofeach group under siudy

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Article
Activities of Some Purine Metabolic Enzymes in Sera and Tissues Homogenates of patients with Different Cervix and Uterine Diseases

Authors: Shatha A. Al-Shammaree --- Hathama R. Hasan
Journal: Medical Journal of Babylon مجلة بابل الطبية ISSN: 1812156X 23126760 Year: 2005 Volume: 2 Issue: 4 Pages: 424-439
Publisher: Babylon University جامعة بابل

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Abstract

Sone aspects of purine metabolism including the activities of some enzymes involved iD purinenucleotides metabolisn: Adenosine deaminase (ADA), AMP- deaminase (AMPDA), 5'-Nocleotidase (5'-NT) and xanthiDe oxidase (XO) were srudied in human sera and tissues ofhealthy women (!$ed as nornralcontrol) and palients with different diseases as follow: Chronic cervicities and uterine sdenomyosis (wlichlvere used as pathological conlrols),benign uterine 1 mors, Prcmal;gnancy group (which represerrr parienEwith chronic cewicilies with mild lo moderate hyperplasia), a d ceryix and utefus cancer.Seveml connnon diflerences in the obtained data, which expressed ir felatioD to prolein contenl.were evident anrong the above groups. The rcsults indicalc tlat thefe were a sigDificant decrease irL sera,ADA and AMPDA specific activiiies of patienls with cervix and terus cancer conpafed with thal ofcontrol, pathological conffol (nalnely: chrooic cervicitis and uterine adenornyosis). benign uterine tLmo,,and premalignancy group(P<0.001). while there were significant increases in ADA and AMPDA specjficactivilies oftissue homogenates ofpalients with cewix and uterus cancer when compared wilh thal of thesanc above meniioned groups (P<0.0J).As fhr as 5'-NT specific activilies was concened, significant increases were obseNed in sera andLissues homogenales ofpatients with cervix and uterus cancer as compared to the othef groups (P <0.01).The dehydrogeuase activities of XO in sem and tissues homogeDates of patients wilh cervix anduterus cancef show a significant decfcase (P <0.05) in comparison to thai ofother gfoups, while thcre wereno derectable oxidase activities ofXo in sefa ofcontrol ard patients with uterus caDce

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Article
Serum lipid peroxidation and trace elements levels in ovarian cancer patients before and after cisplatin and doxorubicin chemotherapy

Authors: Shatha A. Al-Shammaree --- Hathama R. Hasan --- Thikra H. Mathekoor
Journal: Journal of College of Education مجلة كلية التربية ISSN: 18120380 Year: 2011 Issue: 5 Pages: 119-136
Publisher: Al-Mustansyriah University الجامعة المستنصرية

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Abstract

Background: chemotherapy and radiation therapy are associated with formation of reactive oxygen species and depletion of critical serum and tissue antioxidants. Although the oxidative statues in patients undergoing chemotherapy treatment were investigated in many studies, but their results were heterogeneous.Objective: This is a preliminary study conducted to explore the effects of cisplatin and doxorubicin on serum malondialdehyde levels (MDA) (as an oxidative stress indicator) together with the changes in levels of certain trace elements: Zinc (Zn), Copper (Cu), Manganese (Mn), and Iron (Fe) , Cu/Zn ratio, and protein concentration in ovarian cancer patients.Methodology: This is a case control study conducted in Chemistry Department/ College of Science / University of Baghdad. Six patients having histologically confirmed ovarian cancer(stage III) were participated in this study before and after chemotherapy with cisplatin and doxorubicin. Serum MDA was measured by Hirayama et.al spectrophotometry method, and the levels of certain serum trace elements: Zinc (Zn), Copper (Cu), Manganese (Mn), and Iron (Fe) were estimated by using flame atomic absorption spectrophotometry . Also serum protein concentration was measured in both mentioned groups. Three of our six patients were followed –up after the second course of the same chemotherapy where the same parameters were measured.

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