research centers


Search results: Found 28

Listing 1 - 10 of 28 << page
of 3
>>
Sort by

Article
Imipenem-Resistant Acinetobacter baumannii isolated from patients and hospitals environment in Baghdad
بكتريا Acinetobacter baumannii المقاومة للأمبينيم المعزولة من مرضى و بيئة مستشفيات بغداد

Loading...
Loading...
Abstract

During 2011, 1900 clinical specimens and 240 hospital environment specimens were collected from four hospitals in Baghdad. 128 isolates of Acinetobacter baumannii were obtained from clinical and environmental specimens in a percentage of 6.05% and 5.42%, respectively. The highest percentage of isolation, 38.26% was of sputum specimens and lower percentage of burns specimens 5.22%. The lowest incidence was of age range (71-80) years old group whereas the highest incidence was of age range (31-40) years old group. Also we found that the incidence was higher in males (66.96%) than that of females (33.04%) and the frequency of positive A. baumannii isolates was higher in intensive care units (ICUs). Results revealed eleven different resistotype patterns* designated arbitrarily from A-K and all our isolates showed multidrug resistance to those antibiotics. We found the highest percentage (85%) of imipenem resistant A. baumannii (IRAB) isolates were isolated from blood disease (leukemia) department followed by ICU, RCU, Burns, surgical and other departments, respectively.

خلال عام 2011 ، 1900 عينة سريرية و240 عينة من بيئة المستشفيات تم جمعها من مستشفيات في بغداد ،تم الحصول على 128 عزلة تعود الى بكتريا A. baumanniiوبنسبة 6.05% من العينات السريرية و %5.42 من العينات البيئية. أعلى نسبة عزل لهذا النوع وصلت الى 38.26 %تم الحصول عليها من نماذج القشع بينما اقل نسبة عزل تم الحصول عليها من نماذج الحروق ووصلت الى 5.22% من 115 عزلة سريرية. كانت أقل نسبة أصابات بين مجموعة الأعمار71-80 سنة بينما سجلت أعلى نسبة أصابات بين مجموعة الأعمار31-40 سنة وكانت نسبة الأصابة في الذكور (66.96%) أعلى منها في الإناث (33.04%) و أعلى تكرار للأصابة ببكتريا A. baumannii كان في وحدات العناية المركزة. أظهرت النتائج 11 نمطا للمقاومة سميت من K-Aوكانت جميع العزلات مقاومة للعديد من المضادات الحياتية ،فيما يخص العزلات السريرية (115) كانت جميع العزلات متعددة المقاومة. تم تسجيل أعلى نسبة عزل وصلت الى (85%) للعزلات المقاومة لمضاد الأمبنيم من ردهات امراض الدم تلاها وحدات العناية المركزة، وحدات العناية التنفسية، ردهات الحروق، الردهات الجراحية والردهات الأخرى.


Article
Improving of Antibacterial Activity for Antibiotics by Purified and Characterized Lectin from Acinetobacter baumannii

Author: Sahira Nsayef Muslim
Journal: Iraqi Journal of Biotechnology المجلة العراقية للتقانات الحياتية ISSN: 18154794 Year: 2015 Volume: 14 Issue: 1 Pages: 9-22
Publisher: Baghdad University جامعة بغداد

Loading...
Loading...
Abstract

Abstract: Lectin has a wide range of applications in many fields as anti-tumor, anti-insect , antiviral and antifungal drug. Here we reported lectin production from Acinetobacter as there is no report on lectin production from Acinetobacter. In the present study a novel strain, Acinetobacter baumannii S3 isolated from sputum samples gave the highest production level of lectin by microscopic glass slide and microtiter plate methods and the hemagglutination activity was not blood-type specific. Lectin was purified to homogeneity with a recovery yield of 72.72% and 40.76 fold of purification by using ammonium sulfate at 35% saturation followed by QAE-Sephadex ion exchange chromatography and sephadex G-200 gel filtration chromatography. The optimum activity of purified lectin was found at 30ºC and pH 7. The best cofactors for lectin activity were Mn++ and Mg++ with relative activities of 132 and 122%, respectively and severely inhibited in presence of Fe3+, Cu2+and detergents. Lectin led to increase antimicrobial activity of antibiotics and had synergism effect, thus lectin may be useful adjuvant agent for the treatment of many bacterial infections in combination with antibiotics.


Article
Study of Antibiotic Resistance of Acinetobacter baumannii in Intensive Care Units (I.C.Us) and Burn Patients

Author: Saif Adel Al-Taliby , Wathiq Abbas Hatite Al-Daraghi
Journal: Iraqi Journal of Biotechnology المجلة العراقية للتقانات الحياتية ISSN: 18154794 Year: 2019 Volume: 18 Issue: 1 Pages: 32-36
Publisher: Baghdad University جامعة بغداد

Loading...
Loading...
Abstract

The genus Acinetobacter is a major cause of nosocomial infections; it is increasingly being associated with various epidemics and has become a widespread concern in a variety of hospitals worldwide. Multi-antibiotic resistant Acinetobacter baumannii, is now recognized to be of great clinical significance. Numerous reports relay to the spread of A. baumannii in the hospital settings which leads to enhanced nosocomial outbreaks associated with high death rates. However, many other Acinetobacter spp. also can cause nosocomial infections. This study focused on the role of Acinetobacter spp. as nosocomial pathogens in addition to their persistence, antimicrobial resistance patterns and epidemiology.


Article
11.DETECTION OF SOME BIOFILM GENES RELATED WITH MULTIDRUG-RESISTANT IN ACINOBACTER BAUMANNII ISOLATED FROM CLINICAL ISOLATES

Authors: Sura S. Talib سرى سعد طالب --- Thanaa R. Abdulrahman ثناء رشيد عبد الرحمن --- Shatha H. Ali شذى حسين علي
Journal: IRAQI JOURNAL OF MEDICAL SCIENCES المجلة العراقية للعلوم الطبية ISSN: P16816579,E22244719 Year: 2018 Volume: 16 Issue: 4 Pages: 430-438
Publisher: Al-Nahrain University جامعة النهرين

Loading...
Loading...
Abstract

Background: Acinetobacter baumannii (A. baumanii) has recently emerged as a major pathogen causing nosocomial infections in patients admitted to intensive care units with a surprisingly rapid acquisition of antibiotic resistance.Objective: To study the rate of occurrence of A. baumanii in different clinical samples and to investigate the association between biofilm formation and presence of ompA and bap genes in multi-drug resistance isolates.Methods:A total of 150 clinical samples were collected from (blood, sputum, urine, wound swab) during a period from the first of October 2017 to the end of March 2018 from Al-Imamein Al-Kadhimein City, Central Teaching Hospital of Pediatrics, Welfare Children Protection in Medical City and Al-Yarmouk Teaching Hospital and tested against 14 antibiotics by disc diffusion method. Quantitative microtiter plate assay was done for detection of biofilm formation. Polymerase chain reaction (PCR) was performed to detect ompA and bap genes.Results: There were 75 A. baumanii isolated from different clinical samples as follows: 41 from blood, 13 from wound, 12 from sputum and 9 from urine. The results of antimicrobial susceptibility test showed, high rate of resistance to Aztronem (94.7%) followed by Cefotaxime (89.3%), Cefepim (86.7%), Meropinem (86.7%), Ceftriaxone (86.7%), Ceftazidime (85%), Gentamicin (85%), and Piperacillin (82.7%) respectively. Moderate - to - low rate of resistance to Ciprofloxacin (78%), Impenim (46.7%), Levofloxacin (46%), Amikacin (44%), Tigacycline (42.3%) and Colistin (44%). The detection of biofilm formation showed that (52%) of isolate produce biofilm and the prevalence of ompA gene was 86.7% while the prevalence of Bap-gene was 34.7%.Conclusion: High frequency of A. baumannii infection was observed in different hospitals in Baghdad. More than half of the isolates were biofilm producer and there is highly significant association between the presence of bap gene and the biofilm formation but not with ompA gene.Keywords: Acinetobacter baumannii, ompA, Bap, MDCitation: Talib SS, Abdulrahman TR, Ali SH. Detection of some biofilm genes related with multidrug-resistant in Acinobacter baumannii isolated from clinical isolates. Iraqi JMS. 2018; 16(4): 430-438. doi: 10.22578/IJMS.16.4.11

Keywords

Acinetobacter baumannii --- ompA --- Bap --- MD


Article
Molecular Characteristics of Multidrug Resistant Acinetobacter baumannii Isolated from Baghdad Hospitals
الخصائص الجزيئية لبكتريا Acinetobacter baumannii متعدده المقاومة للمضادات الحيوية والمعزولة من مستشفيات بغداد

Authors: Khitam K. AL-Masoudi ختام خالص المسعودي --- Jinan M. AL-Saffar جنان محمد الصفار --- Nuha J. Kendla نهى جوزيف قندلا
Journal: Iraqi Journal of Science المجلة العراقية للعلوم ISSN: 00672904/23121637 Year: 2015 Volume: 56 Issue: 2B Pages: 1394-1399
Publisher: Baghdad University جامعة بغداد

Loading...
Loading...
Abstract

A total of 100 clinical sample from (urine, sputum and swabs of wound , burn and ear) were collected from patients in different hospitals of Baghdad during the period from December 2013 to May 2014. 15 isolates (15%) identified belong to Acinetobacter baumannii, swabs of wounds were represented in high percentage of A.baumannii isolates (40%) while percentage of other samples were variable. Susceptibility of 15 A.baumannii isolates were tested toward 16 different Antimicrobial agents, the results showed all isolates were multi drug resistant. In addition, Polymerase Chain Reaction Technique (PCR) was performed to detection the resistance genes encoding the Oxacillinases enzymes. The PCR analysis showed that the presence of insertion sequence (ISAba1) in 13 isolates whereas blaoxa51like gene was represented in 12 isolates. Furthermore, the results of detection other genes were not appear any amplification for all A.baumannii isolates with genes encoded for bla oxa 58 and bla oxa 143 enzyme.

شملت الدراسة 100 عينه سريريه ومن مصادر مختلفة (الإدرار،القشع،مسحات الجروح،مسحات الحروق ومسحات الإذن) من مرضى مستشفيات مختلفة في بغداد، خلال الفترة الممتدة من كانون الأول 2013 حتى مايس 2014.،شخصت 15 عزله(15%) تعود إلى Acinetobacter baumannii وكانت أعلى نسبه تشخيص تعود إلى مسحات الجروح(40%) وتغايرت نسبه العزل في مصادر العينات الأخرى.اختبرت حساسية عزلات بكتريا Acinetobacter baumannii باتجاه 16 مضاد حيوي، أظهرت نتائج الاختبار أن جميع العزلات كانت متعدده المقاومةللمضادات الحيويه. استخدم تقنيه Polymerase Chain Reaction Technique (PCR) للتحري عن امتلاك العزلات قيد الدراسة على الجينات التي تشفر إلى g(Oxacillinase) فوجد 13 عزله تشفر للتسلسل ISAba1، بينما 12 عزله كانت تحتوي على bla oxa 51 gene إما bla oxa58 gene and bla oxa 143 gene أظهرت نتيجة سالبه لكل العزلات.


Article
Effects of green laser therapy on healing of infected wound in mice.
ثاثير العلاج بالليزر الاخضر على التهام الجروح عند الفيران

Authors: Mawada .M. Funjan موده موسى فنجان --- Fareed F. Rasheed فريد رشيد --- , Alaa Nazar الاء نزار
Journal: IRAQI JOURNALOF COMMUNITY MEDICINE المجلة العراقية لطب المجتمع ISSN: 16845382 Year: 2017 Volume: 30 Issue: 2 Pages: 101-108
Publisher: Al-Mustansyriah University الجامعة المستنصرية

Loading...
Loading...
Abstract

Abstract:Background: Little studies focused on stimulation of healing process of infected wound in mice. The predominant pathogens that infected wound were gram positive such as Staphylococcus aureusand gram negative such as P. aeruruginosa and Acinetobactor Baumannii. A. baumannii cause wide range nosocomial infections. Objective: study the influence of green laser 532nm at constant power density (irradiance) at different exposure times (5, 15) on healing of wound infected by Acinetobacter Baumannii created on BALB/C mice.Methods: An elliptical full thickness skin wound was created aseptically on a back of 45 adults, females BALB/C mice. The wounds were infected by Acinetobacter Baumannii and the mice were randomly divided into two sets, first one was infected ( non-irradiated controls)15 animals (five in each subgroup)dividing according to the days of irradiation, second set was infected(irradiated groups)randomly divided in to two groups, first group irradiated for 5 mints and second group irradiated for 15 minutes. Both irradiated groups subdivided according to follow up period 3, 5, 10 days. Animal killed on day 3, 5, and 10 after treatment and control group killed also on 3,5,10 after contamination. Cold laser therapy (CLT) achieved by semiconductor diode laser 532nm, output power =40mw, continuous wave laser, power density =5.71 mw/cm2 and doses 1.7J/cm2, 5.14J/cm2. The output of green laser fitted with beam expander to irradiate a circular area of diameter 7cm2. CLT started after surgery and repeated for 3, 5, and 10 days. Wound healing studied by calculating the percentage of wound closure and histopathological evaluation. Conclusion: the present study showed that green laser therapy had obvious influence on healing of infected wound especially with a dose 5.14 J/cm2.


Article
Clinical Risk Factors for Nosocomial Infection Caused by Acinetobacter baumannii among Iraqi Patients suffering from differing burns

Author: Zahraa Falah Azeez , Wathiq Abbas Hatite Al-Daraghi
Journal: Iraqi Journal of Biotechnology المجلة العراقية للتقانات الحياتية ISSN: 18154794 Year: 2018 Volume: 17 Issue: 3 Pages: 61-66
Publisher: Baghdad University جامعة بغداد

Loading...
Loading...
Abstract

This study aimed to evaluate risk factors and the role of Acinetobacter baumannii isolates in mortality due to burns since morbidity and mortality rates are considerably high. A total of 204 patients hospitalized in Burn Care Unit at Al-Diwanyia Teaching Hospitial, The Burn Specialist Hospital between January 2017 and February 2018 were reviewed retrospectively. Logistic regression analysis was used in order to predict the risk. RESULTS: From the collected notes, a total of 289 bacterial colonies were isolated of these patients and from them 22 isolates were identified as A. baumannii. Mortality rates were as follows: 10.5% in general, 4.9% for fewer than 17 years of age, and 5.6 % for between 18-64 years of age.


Article
In silico Modeling of Beta-lactamase Protein Acinetobacter baumannii

Author: Taif M. Abdul-Hussein1 , Hasan M. Abo Almaali2 , Shurook M.K. Saadedin1 , Wifaq M. Al-Wattar3
Journal: Iraqi Journal of Biotechnology المجلة العراقية للتقانات الحياتية ISSN: 18154794 Year: 2019 Volume: 18 Issue: 1 Pages: 37-42
Publisher: Baghdad University جامعة بغداد

Loading...
Loading...
Abstract

The most common mechanism of resistance to β-lactam antibiotics in gram-negative bacteria is the production of β-lactamases that hydrolyze the drug. Results that have been obtained from some servers that have been used in this study were gave a poor quality and prediction. SWISS MODEL server results gave more promising result as it had 92% query coverage. Validated was done for the model study by using QMEAN score, Ramachandran plot and ProSA server. 3D Refine and Mod Refiner were used for model refinement. Finally, ProSA server have been used in order to revalidate the model.


Article
Evaluation of Some Material to inhibit Biofilm Formed by Acinetobacter baumannii Isolates
تقييم بعض المواد في تثبيط الغشاء الحيوي المتكون من قبل عزلات Acinetobacter baumannii

Authors: Ghada A. Mohammad غادة عبد الرزاق محمد --- Noor Hussein Ahmad نور حسين احمد
Journal: Tikrit Journal of Pure Science مجلة تكريت للعلوم الصرفة ISSN: 18131662 Year: 2019 Volume: 24 Issue: 4 Pages: 19-24
Publisher: Tikrit University جامعة تكريت

Loading...
Loading...
Abstract

The study has included the ability of Acinetobacter baumannii (isolated from clinical infections and hospital environment) to form a biofilm and investigated the possibility of inhibition this biofilm by using some materials: EDTA, five amino acids and five plant extracts.The results showed that all isolates of A.baumannii had a good ability to form the biofilm (100%). Four isolates which had strong former of biofilm which were selected as well as a standard isolate (A. baumannii ATCC19606). The inhibitory effect on biofilm formation was investigated. EDTA had a good inhibitory effect, also, all amino acids showed good inhibitory activity , with the best inhibition in Glutamic acid at a concentration of 50 mM, while plant extracts varied in their inhibition ratio to inhibit the biofilm, turmeric, cloves and rosemary had a good inhibitory effect, Bay leaf has the ability to inhibit the biofilm formation but less than others, while the plant extract of the Myrtle did not show any inhibition activity.

تضمنت الدراسة قابلية عزلات A.baumannii المعزولة من الاصابات السريرية وبيئة المستشفيات على تكوين الغشاء الحيوي والتحري عن إمكانية تثبيط هذا الغشاء الحيوي باستخدام بعض المواد كالـ EDTA وخمس أحماض أمينية وخمسة مستخلصات نباتية. أظهرت النتائج أن جميع عزلات A.baumannii لديها قدرة جيدة على تكوين الأغشية الحيوية بنسبة 100٪. انتخبت أربع عزلات من البكتيريا التي أظهرت أعلى نسبة تكوين للغشاء الحيوي فضلاً عن استخدام العزلة القياسية وتم التحري عن تثبيط الغشاء الحيوي فيها, اظهر EDTA تأثير تثبيطي جيد، كما أظهرت جميع الأحماض الأمينية فعالية تثبيطيه جيدة في تثبيط الغشاء الحيوي مع أفضل تثبيط في حامض الغلوتاميك بتركيز 50 mM، في حين تباينت المستخلصات النباتية في نسبة تثبيطها للغشاء الحيوي, اذ اظهر كل من الكركم والقرنفل وإكليل الجبل تأثير تثبيطي جيد، بينما كان لاوراق الغار قدرة تثبيطيه قليلة، في حين أن المستخلص النباتي لنبات الاس لم يظهر أي فعالية تثبيطيه.


Article
Physicochemical Factors Affected the Partial Purified Lipase Activity of Acinetobacter baumannii “local isolates”
لعوامل الفزيوكيمائية المؤثرة على فعالية انزيم اللايبيز المنقى جزئيا من جرثومة Acinetobacter baumannii

Author: Huda J. Mohammed هدى جاسم محمد
Journal: Iraqi Journal of Pharmaceutical Sciences المجلة العراقية للعلوم الصيدلانية ISSN: 16833597 Year: 2013 Volume: 22 Issue: 1 Pages: 82-89
Publisher: Baghdad University جامعة بغداد

Loading...
Loading...
Abstract

Microbial lipases today occupy a place of prominence among biocatalysts owing to their ability to catalyze awide variety of reactions in aqueous and non- aqueous media, A.baumannii were isolated from different clinical specimens from hospitalized patients from Baghdad hospitals and were detected by biochemical tests and API20E system. The percentage of isolation was (16.6%), A. baumannii is an increasingly multidrug – resistant (MDR), it showed high level of resistant to Ceftriaxon, Colistin, Piperacillin, Co-trimoxazol, Tertracycline, Carbenicillin, Amoxicillin, Penicillin G, Gentamicin and Ceftazidim , wherease the isolates were highly sensitive to Imipenem, Ciprofloxacin, Meropenem, Amikacin, and Cefotaxime. The isolated strians of A.baumannii were screened for the production of lipase by using Rhodamine B agar media. one of the isolated strians exhibited a greater clear zone than the others, indicated higher lipase activity. The lipase in present study was partially purified by ammonium sulphate at the concenterations of 30% and 80%, the 30% ammonium sulphate showed higher activiy of the enzyme than 80% in which the enzyme activity was slightly decreased indicating that the better purification was at the 30% concenteration of ammonium sulphate.Various physicochemical parameters such as pH, temperature and metal ions were studied in order to determine the optimum conditions for lipase production. The production of lipase by A. baumannii was optimum at 35C, pH 7.0 and was enhanced by the Ca++ and Na+ wherease inhibited by Zn++ ions.

انزيمات اللايبيزالمايكروبية تحتل اليوم مكانة مهمة لدورها الكبير كمحللات حياتية في الاوساط السائلة وغير السائلة , تم عزل البكتريا من نماذج سريرية مختلفة من مرضى راقدين في مستشفيات مختلفة في بغداد وتم تشخيصها بالاعتماد على الاختبارات الكيموحياتية ونظام(API20E) وكانت نسبة العزل 16,6 %, أن هذه البكتريا واسعة المقاومة للمضادات الحياتية وكانت منها :-(Ceftriaxon, Colistin, Piperacillin, Co-trimoxazol, Tertracycline, Carbenicillin, Amoxicillin, Penicillin G, Gentamicin and Ceftazidim) . في حين كانت حساسة للمضادات (Imipenem, Ciprofloxacin, Meropenem, Amikacin, and Cefotaxime). تم اختبار قابلية البكتريا المعزولة على انتاج أنزيم اللايبيز باستخدام وسط (Rhodamine B agar) وكانت عزلة واحدة فقط منتجة للأنزيم بشكل فعال, تمت التنقية الجزئية لأنزيم اللايبيز بأستخدام طريقة الترسيب بكبريتات الأمونيوم بتركيز 30% و80%,وأظهر التركيز 30% من كبريتات الأمونيوم فعالية أنزيمية عالية اعلى من تلك عند التركيز 80% وهذا يشير الى ان عملية التنقية الجزئية تكون افضل عند التركيز 30% من كبريتات الأمونيوم.تم دراسة الظروف الفزيائية والكيميائية التي تؤثر على فعالية الأنزيم وتبين ان الرقم الهدروجيني الأمثل لأنزيم اللايبيز هو 7 ودرجة الحرارة المثلى هي 35 سيليزية وتبين ايضا ان فعالية الأنزيم تتحفز بوجود ايونات الكاليسوم وايونات الصوديوم وتتثبط الفعالية بوجود ايونات الخارصين. .

Listing 1 - 10 of 28 << page
of 3
>>
Sort by
Narrow your search

Resource type

article (28)


Language

English (26)

Arabic (2)


Year
From To Submit

2019 (6)

2018 (9)

2017 (4)

2016 (2)

2015 (3)

More...