research centers


Search results: Found 8

Listing 1 - 8 of 8
Sort by

Article
Bronchiectasis in northern lraq clinical and bacteriological characteristics during acute exacerbation

Author: Rami M. A. Al-Hayali رامي محمد عادل الحيالي
Journal: Annals of the College of Medicine Mosul مجلة طب الموصل ISSN: 00271446 23096217 Year: 2005 Volume: 31 Issue: 1 Pages: 10-16
Publisher: Mosul University جامعة الموصل

Loading...
Loading...
Abstract

Context: Bronchiectasis is a relatively common disease in developing countries, The pathogens responsible for its acute exacerbations vary from one area to another.Objectives: To study the characteristics of patients with bronchiectasis in Northern lraq, and to identify the types of pathogens responsible for the acute exacerbations.Design: Case series study.Setting: Respiratory Care Unit and general medical wards in lbn-Sina Teaching Hospital inMosul, during the years 2002-2004.Patients and Methods: Fifty patients with bronchiectasis (16 males and 34 females) presenting during an acute exacerbation underwent clinical and radiological evaluation with sputum Gram (Gm)stain and culture.Results: The cause of the disease was readily identifiable in 367o of patients. Tuberculosis was the predominant aetiology. Streptococcus pneumoniae and klebsiella pneumoniae were the commonest pathogens. Gm negative bacilli were responsible for 36% of cases overall, and were especially important in those with long standing disease (≥15 years) Pseudomonasaeruginosa was related to more extensive disease. Most of the isolates were sensitive tociprofloxacin and cefotaxime.conclusion: Gm negative bacilli should be considered in antibiotic selection during acuteexacerbation of bronchiectasis, especially in long standing and extensive disease.Keywords: bronchiectasis, Gram negative bacilli


Article
Assessment of Bacterial Contamination of Orthodontic Arch wire

Authors: Suha Saad Hassan --- Nidhal H. Ghaib --- Batool Hassan Al-Ghurabi
Journal: Journal of baghdad college of dentistry مجلة كلية طب الاسنان بغداد ISSN: 16800087 Year: 2019 Volume: 31 Issue: 1 Pages: 48-51
Publisher: Baghdad University جامعة بغداد

Loading...
Loading...
Abstract

Background: The microorganisms can impend the life of health care professional and particularly the dental practitioners. They can be transmitted by different ways like airborne and droplet transmission. The current study was carried out to identify whether the arch wires that received from the manufactures are free from microbial contamination and to determine the bacterial species attached to the arch wires.Materials and Methods: This study involved eighty samples, consisted of two types of arch wires (nitinol and stainless-steel) from four companies (3M, G&H, Jiscop, OrthoTechnology). These wires inserted in a plane tube that contains 10 -ml of (Tris [tris(hydroxymethyl)aminomethane] and EDTA (ethylenediaminetetraacetic acid) tris-EDTA and brain heart infusion (BHI) broth. A 0.1 ml was withdrawn from the tube and spread on agar plates. The control groups consist of 16 plane tube (8 tubes with tris-EDTA and other 8 tubes with (BHI).Results: Microbial sampling yielded growth from 5 of the 80 arch wires. The predominant bacteria that isolated were Bacillus spp. No growth was recovered from 75 of the samples and from controls. The bacteria were isolated by BHI reagent and no growth was observed by tris-EDTA reagent with statistically significant difference (P<0.05). The Bacillus spp. found only in the G&H and Jiscop companies, however, no statistically significant difference was found among them (P>0.05). With regard to the presence and distribution of bacteria according to the types of wires, the present results clarified that cases of contamination with Bacillus spp. were found in the nitinol arch wires with statistically significant difference (P<0.05).Conclusions: The results of the current study revealed low count of bacterial contamination in the two types of companies (G&H and Jiscop). Not all materials that received from the manufactures are free from contamination and an effective sterilization regimen is needed to avoid cross-contamination.

Keywords

Arch wires --- contamination --- Bacilli


Article
Erythrocyte sedimentation rate in patients with positive sputum for AFB and negative HIV serological test

Author: Ahmad Hasan Raheem
Journal: Muthanna Medical Journal مجلة المثنى الطبية ISSN: 2226146x Year: 2016 Volume: 3 Issue: 1 Pages: 17-23
Publisher: Al-Muthanna University جامعة المثنى

Loading...
Loading...
Abstract

The goals of this study are detection of the prevalence of ESR elevation in patients whosuffering from pulmonary tuberculosis with positive sputum for acid fast bacilli and negative serological test for HIV, and showingthe effect of normal ESR ondiagnosis of active pulmonary tuberculosis. 37 patients of pulmonary TB with sputum positive for AFB and negative serological test for HIV, were involved in this retrospective study over145 days,at major TB center of Al-Hillacity, Iraq.The Westergren method used for detection of ESR for all patients. Of the total 37 patients involved in this study 21(57%) were males and 16(43%) were females. The ages of the male patients ranged from 21-82 years, and the females from 23-75 years in addition to one girl with age of 5-year. 22(59.5%) patients cigarette smoker and 15(40.5%) non smoker. 3 patients (8.1%) with ESR less than 20mm/hr, 13 patients (35.1%) with ESR 20-40mm/hr,12 patients (32.4%) with ESR 41-60mm/hr and 9 patients (24.3%) more than 60 mm/hr. According to dependent normal range in this study 7(19 %) patientswith normal ESR. 19% of patients involved in this study have normal ESR, so the pulmonary TB should be expected in any patient with suggestive clinical features even when the ESR is normal specially in an endemic area as in Iraq, and it is clear from result above that the smoking is important risk factor for pulmonary TB.

Keywords

ESR --- Tuberculosis --- Acid Fast Bacilli --- Sputum --- HIV


Article
Does Normal Chest X ray in Patients with Chronic Cough Exclude Pulmonary tuberculosis?

Authors: Mohammad Yahya Abdulrazaq --- Abdulla Janger Minshed
Journal: Iraqi Academic Scientific Journal المجلة العراقية للاختصاصات الطبية ISSN: 16088360 Year: 2011 Volume: 10 Issue: 1 Pages: 130-133
Publisher: The Iraqi Borad for Medical Specialization المجلس العراقي للاختصاصات الطبية

Loading...
Loading...
Abstract

ABSTRACT:BACKGROUND:Tuberculosis is the second infection in causing deaths from infectious agent in the world, currently in Iraq approximately 67% of new cases of Tuberculosis involve the lung only ,cough is the most common symptom of pulmonary Tuberculosis , pulmonary Tuberculosis nearly always causes detectable abnormalities on chest film, but still atypical or absent radiologic findings can occur.OBJECTIVE:This study was designed to determine whether in patients with chronic cough normal chest X ray exclude pulmonary tuberculosis.PATIENTS AND METHODS:One hundred seventy two patients attending the respiratory clinic, complaining from chronic cough and they are suspected cases of tuberculosis, were enrolled in this study. A full medical history and physical examination was done then a chest X ray was ordered, for those with normal chest X ray finding (seventy seven patients), sputum smear for acid fast bacilli (AFB) ordered .RESULTS:47 females (61%), 30 males (39%) with chronic cough with females to male ratio = 1.56/1.The age ranged between 17-67, with mean age of 37.16 years. The age of males ranged between 18-67 and the mean was 39.32 years, the age of females ranged between 17-66 and the mean was 34.93 years. X ray finding were negative in all the patients. Positive AFB in sputum smear examination by microscopy was found in only one patient but with ENT examination it was proved to be a case of laryngeal tuberculosis not pulmonary tuberculosis.CONCLUSION:Normal chest x ray in patients with chronic cough excludes pulmonary tuberculosis


Article
Resistance of some Gram Negative Enteric Bacilli Isolated from Lower Respiratory Tract Infections to β-lactam Antibiotics
مقاومة بعض العصيات المعوية السالبة لصبغة كرام المعزولة من إصابات الجهاز التنفسي السفلي لمضادات البيتا-لاكتام

Authors: Mahmood Z. Al-Hasso محمود زكي الحَسّو --- Subhi H. Khalaf صبحي حسين خلف صبحي حسين خلف
Journal: Rafidain journal of science مجلة علوم الرافدين ISSN: 16089391 Year: 2013 Volume: 24 Issue: 6A Pages: 66-79
Publisher: Mosul University جامعة الموصل

Loading...
Loading...
Abstract

One hundred and four isolates of gram negative enteric bacilli were isolated from (700) sputum specimens taken from lower respiratory tract infections with isolation percentage of (14.8%). The bacterial isolates belonged to the species: Pseudomonas aeruginosa, Klebsiella pneumoniae, Escherichia coli, Enterobacter aerogenes, Enterobacter cloacae, Serratia marcescens, Proteus mirabilis, with variable isolation percentages. The isolates showed an obvious resistance to different β-lactam antibiotics including third generation cephalosporins, where the resistance percentages to Cefixime, Cefotaxime, Ceftazidime reached (52.9%), (51.9%), (42.3%) respectively, they also showed resistance to Cefoxitin (31.7%), while Cefepime, Meropenem, Imipenem had a high activity against the isolates, as the resistance rates were reduced to reach (8.7%), (1.9%), (1.0%) respectively. Ps.aeruginosa isolates were the most resistant species to β-lactams, followed by K.pneumoniae isolates, while Pr. mirabilis isolates were the most susceptible ones.

عزلت (104) عزلة من العصيات المعوية السالبة لصبغة كرام من مجموع (700) عينة قشع مأخوذة من إصابات الجهاز التنفسي السفلي، وبنسبة عزل (%14.8)، وكانت العزلات تابعة لأنواعPseudomonas aeruginosa ,Klebsiella pneumoniae ,Escherichia coli ,Enterobacter aerogenes ,Enterobacter cloacae ,Proteus mirabilis ,Serratia marcescens، وبنسب عزل متباينة. أظهرت العزلات مقاومة واضحة لمضادات البيتا-لاكتام بأنواعها المختلفة بما فيها سيفالوسبورينات الجيل الثالث، حيث بلغت نسب المقاومة لمضادات: Cefixime، Cefotaxime، Ceftazidime (%52.9)، (%51.9)، (%42.3) على التوالي، كما أظهرت العزلات مقاومة لمضاد Cefoxitin بنسبة (%31.7)، في حين كان لمضادات: Cefepime، Meropenem، Imipenem فعالية عالية ضد هذه العزلات إذ انخفضت نسب المقاومة لها لتبلغ (%8.7)، (%1.9)، (%1.0) على التوالي. وكانت عزلات جرثومة Ps. aeruginosa أكثر الأنواع الجرثومية مقاومة لمضادات البيتا-لاكتام، تلتها عزلات جرثومة K. pneumoniae، في حين كانت عزلات جرثومة Pr.mirabilis أكثر الأنواع حساسية لهذه المضادات.


Article
GeneXpert MTB/RIF Assay – A Major Milestone for Diagnosing Mycobacterium tuberculosis and Rifampicin-Resistant Cases in Pulmonary and Extrapulmonary Specimens

Author: Shatha Thanoon Ahmed
Journal: Medical Journal of Babylon مجلة بابل الطبية ISSN: 1812156X 23126760 Year: 2019 Volume: 16 Issue: 4 Pages: 297-301
Publisher: Babylon University جامعة بابل

Loading...
Loading...
Abstract

Background and Objective: Tuberculosis (TB) is an endemic disease in Iraq. Many methods are available to diagnose pulmonary andextrapulmonary TB (EPTB). The most traditional test is the sputum smear for acid‑fast bacilli (AFB). However, it is well known for itslow sensitivity and specificity. On the other hand, culturing AFB although considered the gold standard for detecting Mycobacteriumtuberculosis (MTB), yet it takes long time to confirm or exclude the presence of TB. The WHO has recommended the use of a gene‑basedmolecular technique called GeneXpert (GX) MTB/rifampicin (RIF) for rapid and accurate detection of MTB in pulmonary andextrapulmonary (EPTB) sites. GX is a quick, fully automated system that can be easily used with minimal training. The objective of thisstudy was to evaluate the accuracy of the GX test for diagnosing MTB in pulmonary and extrapulmonary sites in Kurdistan/Iraq that isconsidered as an endemic area for TB, as well as testing the ability of this technique to identify the resistant strains of these bacilli to first‑lineanti‑TB treatment. Methodology: A total of 925 (504 males and 421 females) patients attended the TB center in Erbil/Iraq from August 2015to August 2017. These patients were clinically diagnosed or suspected to have TB. Two sputum samples were collected from each patientand subjected to AFB smear staining. The other portions of the sputum were examined by GX assay, and a number of cases were grown onthe Lowenstein–Jensen media. For extrapulmonary fluid samples, the same tests were done. Results: Seven hundred and forty‑three werepulmonary samples, and the remaining 182 cases were extrapulmonary specimens (cerebrospinal fluid, peritoneal aspirate, pleural fluid,urine, and blood). Of these, 575 had their AFB smears done which was positive for in 184 (32%) and negative in 391 (68%) cases. On theother hand, real‑time polymerase chain reaction using GX technology was positive in 228 (39.65%) while negative in 347 (60.34%) cases.The sensitivity and specificity of the GeneX versus AFB smear considering culture as a gold standard were 94.9% and 80.1%, respectively.In addition, GX technique revealed that about 20 (14.3%) of positive MTB cases were resistant to RIF therapy. Conclusion: The currentstudy displayed the real significance of using GX test in diagnosing MTB in pulmonary and extrapulmonary specimens to save time andto avoid unnecessary anti‑TB treatment.


Article
A Comparative Study of Two Staining Methods For The Detection of Tuberculosis Bacilli (TB)
دراسة مقارنة لطرق تلوين اثنين لكشف العصيات السل (TB)

Author: Nada F. Al-Rawi ندىفتحي الراوي
Journal: The Medical Journal of Tikrit University المجلة الطبية لجامعة تكريت ISSN: 18131638 Year: 2007 Volume: 1 Issue: 131 Pages: 60-63
Publisher: Tikrit University جامعة تكريت

Loading...
Loading...
Abstract

The presence of acid fast bacilli in sputum specimen was investigated comparatively with Ziehl-Neelsen (ZN)and Kinyoun Cold staining (KC) in order to determine sensitivity in detecting Tuberculosis Bacilli (T.B.).
This study was employed on patients with severe lower respiratory tract infection to compare between these two stains. Which revealed that Ziehl-Neelsen procedure gives better results than Kinyoun cold procedure.

وقد بحثت عن وجود عصيات حامض سريع في عينة البلغم نسبيا مع تسيل نلسن، (زد) وKinyoun تلطيخ الباردة (KC) من أجل تحديد حساسية في الكشف عن البكتيريا السل (TB).
وكان يعمل هذه الدراسة على المرضى الذين يعانون من انخفاض حاد عدوى الجهاز التنفسي للمقارنة بين هذه البقع اثنين. وكشفت التي


Article
Anti-bactericidal and anti-biofilm activities of silver nanoparticles against multidrug-resistant Gram-negative bacilli isolated from burn wound infections
أنشطة مضادة للجراثيم و للغشاء الحيوي من الجسيمات النانوية الفضة ضد العصيات المتعددة المقاومة الدوائية السالبة لغرام والمعزولة من أخماج جروح الحروق

Author: Issam Jumaa Nasser * عصام جمعة
Journal: Al-Kindy College Medical Journal مجلة كلية الطب الكندي ISSN: 18109543 Year: 2018 Volume: 14 Issue: 1 Pages: 72-77
Publisher: Baghdad University جامعة بغداد

Loading...
Loading...
Abstract

Background: The emergence and spread of multidrug-resistant Gram-negative bacilliin burn wound infections related to biofilm formation, which lend to challenge in treatment with conventional antibiotics andprompting to search for novel antimicrobial agents to control the infections.Silver nanoparticles (AgNPs) have wide spectrum biological properties with different mechanisms of action and less toxicity towards human cells. Objective:The goal of this study was to evaluated the anti-bacterial and anti-biofilm activities of AgNPs alone and in combination with aminoglycoside (Amikacin) and β-lactam (Ampicillin) antibiotics against multidrug resistant Gram-negative bacilli (Pseudomonas aeruginosa, Escherichia coli, klebsiellapneumoniae) isolated from burn wound infections. Type of the study: Cross –sectional study.Methods: 70 clinical isolates of GNBtested for susceptibility tests by disk diffusion method against 10 antibiotics. The minimum inhibitory concentrations (MICs) of AgNPs and antibiotics were carried out according to the standard broth microdilution method, while synergistic interactions were evaluated by time kill-kinetic assays. Calgary method was applied for anti-biofilm activity. Results:Pseudomonas aeruginosa represented the majority of GNBisolated from burn wound infections 34 (48.5 %)followed by Klebsiella pneumonia 21 (30 %) and Escherichia coli 15 (21.5 %). Silver nanoparticles showed remarkable antibacterial activity against GNB that isolated from burn wound infections with the MICs between 25- 75 µg/ml. Aztreonam, amikacin and cefepime were the most effective antimicrobial drugagainst GNB isolates.Synergistic bactericidal effects were observed in two-drug combinations of AgNPswith broad-spectrum aminoglycoside (Amikacin) and β-lactam (Ampicillin) antibiotics against multidrug resistant GNB. In addition,AgNPsalone or in combination with ampicillin inhibited biofilm activity about 60 % – 75 % ofGNB,while combination of AgNPs withamikacin exhibited a powerful anti-biofilm activity and inhibition biofilm formation by 75% to 80%. Conclusion: The results confirmed a synergistic bactericidal effects and significant enhancing of anti-biofilm activity of AgNPs in combination with antibiotics (amikacin and ampicillin) against multidrug resistant GNB isolated from burn wound infections. These data suggest that AgNPs could beapplied as nanodrug for treatment of burn wound infections.

الخلفية: ظهور وانتشار عصيات سلبية الغرام المقاومة للأدوية المتعددة في أخماج الجروح الحرق بشكل غشاء حيوي ، والتي تشكل تحدي في العلاج بالمضادات الحيوية التقليدية، مما دفع للبحث عن مواد جديدة مضادة للميكروبات للسيطرة على العدوى. الجسيمات النانوية الفضة (AgNPs) لها خصائص بيولوجية واسعة الطيف مع آليات عمل مختلفة وأقل سمية تجاه الخلايا البشرية.الهدف: كان الهدف من هذه الدراسة تقييم الأنشطة المضادة للبكتيريا والمضادة للغشاء الحيوي للجسيمات النانوية الفضية وحدها وبالاشتراك مع المضادات الحيوية مثل أمينوغليكوزيد (أميكاسين) و بيتا لاكتام (الأمبيسلين) ضد للعصيات سلبية الغرام المقاومة للعقاقير المتعددة و المتضمنة " الزائفة الزنجارية و كليبسيلا الالتهاب الرئوي والإشريكية القولونية" معزولة من أخماج جروح الحروق.طريقة البحث : 70 عزلة سريرية من عصيات السلبة لغرام تم اختبارها لحساسية المضادات الحيوية عن بطريقة نشر القرص ضد 10 من المضادات الحيوية. تم إجراء تركيزات الحد الأدنى المثبطة من الجسيمات النانوية الفضية والمضادات الحيوية وفقا لطريقة تخفيف المرق القياسية، في حين تم تقييم التفاعلات التآزر من خلال المقايسات القتالية للوقت . تم تطبيق طريقة كالغاري لنشاط المضاد للغشاء الحيوي.النتائج: شكلت الزائفة الزنجارية غالبية عصيات السلبة لغرام معزولة من أخماج جروح الحروق 34 (48.5٪) تليها كليبسيلا الالتهاب الرئوي 21 (30٪) والإشريكية القولونية 15 (21.5٪). وأظهرت الجسيمات النانوية الفضية نشاطا مضادة للجراثيم ملحوظا ضد عصيات السلبة لغرام معزولة من أخماج جروح الحروق مع متوسطة تركيزات الحد الأدنى المثبطة ما بين 25- 75 ميكروغرام / مل. وكان ازتريونام، أميكاسين وسيفيبيمي المضادة للميكروبات الأكثر فعالية ضد العزلات العصيات السلبة لغرام. وقد لوحظت آثار مضادة للجراثيم التآزرية في توليفات الدوائية من الجسيمات النانوية الفضية مع المضادات الحيوية واسعة الطيف مثل الأمبيسلين و أميكاسين ضد عصيات السلبة لغرام المقاومة للمضادات الحيوية. بالإضافة إلى ذلك، الجسيمات النانوية الفضية وحدها أو بالاشتراك مع الأمبيسلين ثبطت نشاط الغشاء الحيوي حوالي 60٪ - 75٪لعصيات السلبة لغرام ، في حين أن مزيج من الجسيمات النانوية الفضية مع أميكاسين أظهرت نشاط قوي لمكافحة الغشاء الحيوي وتثبيطه بنسبة 75٪ إلى 80٪.الاستنتاج: أكدت النتائج تأثيرات مضادة للجراثيم التآزرية وتعزيز كبير لنشاط المضاد للغشاء الحيوي من الجسيمات النانوية الفضية في تركيبة مع المضادات الحيوية (أميكاسين والأمبيسلين) ضد البكتريا العصوية السالبة لغرام المقاومة للأدوية المتعددة والمعزولة من أخماج جروح الحروق. وتشير هذه البيانات إلى أن الجسيمات النانوية الفضية يمكن تطبيقها كدواء نانوي لعلاج التهابات جروح الحرق.

Listing 1 - 8 of 8
Sort by
Narrow your search

Resource type

article (8)


Language

Arabic and English (2)

English (2)

Arabic (1)


Year
From To Submit

2019 (2)

2018 (1)

2016 (1)

2013 (1)

2011 (1)

More...