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Article
Effects of Pimpinella Anisum extract on salivary counts of Streptococci and Mutans Streptococci in comparison to Chlorhexidine in vivo

Authors: Sabreen S. Abd Al- Muhsen صابرين عبد المحسن --- Wesal A. Al-Qbaidi وصال العبيدي
Journal: Journal of baghdad college of dentistry مجلة كلية طب الاسنان بغداد ISSN: 16800087 Year: 2012 Volume: 24 Issue: special issue 1 Pages: 131-134
Publisher: Baghdad University جامعة بغداد

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Abstract

Background: Pimpinella Anisum is one of the commonly utilized traditional medicines due to its pharmaceuticalproperties such as anti-inflammatory, anti-viral, anti-fungal, and anti-bacterial effects. It is used to relieve coughs,sore throats and contraction of epilepsy also used in refreshing mouth and against bad breath.Aim of the study: To test the effects of Pimpinella Anisum extracts on the viability counts of salivary Streptococci andMutans Streptococci in comparison to chlorhexidine in vivo.Materials and methods: Pimpinella Anisum was extracted using ethanol; Different concentrations of the extractswere prepared in gm/100 ml of deionized water. Chlorhexidine used as control positive and deionized water ascontrol neutral. The volunteers were dental student divided into three groups each group rinse with one of the testagent (Pimpinella Anisum extract, chlorhexidine and deionized water) for 1min. Counts of bacteria recorded at fivetime interval (before rinsing, 1min., 15min., 30min. and 1hr). Mutans Streptococci were isolated from stimulated salivaof the students, purified and diagnosed according to morphological characteristics and biochemical tests.Results: Highly significant differences between the extract and both chlorhexidine and deionized water regardingthe count of bacteria. There were no significant differences between extract and chlorhexidine regarding pH, whilesignificant differences were found between the extract and deionized water at all time points except after 30minutes it was highly significant difference.Conclusion: Pimpinella Anisum ethanol extract was effective against Mutans Streptococci


Article
Comparing the effect of probiotic and chlorhexidine as a mouth rinses in bacterial plaque

Authors: Amer Al- Sheikh Thaer --- Alaa Omran الاء عمران
Journal: Journal of baghdad college of dentistry مجلة كلية طب الاسنان بغداد ISSN: 16800087 Year: 2012 Volume: 24 Issue: special issue 2 Pages: 93-99
Publisher: Baghdad University جامعة بغداد

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Abstract

Background: Probiotic technology represents a breakthrough approach to maintaining oral health by utilizing naturalbeneficial bacteria commonly found in healthy mouth to provide a natural defense against those bacteria thoughto be harmful to periodontal tissue. Data are still sparse on the probiotic action in the oral cavity. The aim of studywas the present study evaluated clinically the efficacy of probiotic type inarched yeast extract, and chlorhexidinemouth rinses on plaque.Material &method: Four strain of probiotics bacteria were used, Lactobacillus acidophilus, Lactobacillus rhamnosus,Lactobacillus bulgaricus & bifidubacterium actiregularis, and grown in skim milk and plus 0.25, 0.5 % yeast extractindividually, the cell free extract of each one of those treatments were add 1: 9 todiluted bacterial plaque takenfrom 5 subjects, to measure the effect of cell free extract on aerobic & anaerobic bacteria.45 subjects with chronic periodontitis attend periodontal clinic in college of dentistry university of Baghdad (age 25-35) the trial design as 14 days comparative study between a probiotic and chlorhexidine.Result: add yeast extract increased the total count of 4 probiotic bacteria 16.25 -29.22% , Cell free extract ofLb. acidophilus probiotic skim milk dairy product was the most powerful in reducing both aerobicand anaerobic plaque bacteria among other probiotic bacteria, it reduced 3.38 – 2.17 log cfu/graerobic bacteria while the same treatment reducing the anaerobic bacteria 2.5- 2.1 log cfu/gr ,the probiotic and chlorhexidine groups had less plaque accumulations compared with the control group at the endof 14day (P<0.001and P<0.001)respectively .Conclusion: the probiotic mouth rinse was found effective in reducing plague accumulation and gingivalinflammation, probiotic mouth rinse obviously has a potential therapeutic value and further study is recommendedto determine the efficacy.


Article
The effects of different concentrations of Alum solutions on Mutans streptococci (in vitro study)

Authors: Raed F. AI-Huwaizi رائد الحويزي --- Wael S. Al-Alousi وائل الالوسي
Journal: Journal of baghdad college of dentistry مجلة كلية طب الاسنان بغداد ISSN: 16800087 Year: 2013 Volume: 25 Issue: special issue 1 Pages: 146-151
Publisher: Baghdad University جامعة بغداد

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Abstract

Background: Alum has been used as a treatment medication in cases of oral and gingival ulcers, and also asantiseptic mouthwash. This study aimed to examine the effects of different concentrations of Alum on inhibition zone,viability counts and adherence ability of Mutans streptococci compared with deionized water and chlorhexidinegluconate in vitro.Materials and methods: The study dealt with an in vitro study to establish a concentration of Alum mouthrinse thatwould have the minimum inhibitory concentration and minimum bacteriocidal concentration. The second partevaluated the anti-adherence ability of the experimental agents.Results: This study found that the antibacterial effect of Alum increases with its concentration from 50 to 10000 PPMbut still weaker than 0.1% chlorhexidine gluconate. Only concentrations of 5000 and 10000 PPM showed negativeadherence of Mutans streptococci to the tooth surface.Conclusions: This study found that the antibacterial effect of Alum increases with its concentration from 50 to 10000PPM but still weaker than 0.1% chlorhexidine gluconate. Only concentrations of 5000 and 10000 PPM showednegative adherence of Mutans streptococci to the tooth surface


Article
Gingival microleakage of composite restorations with different bonding protocol in class II cavity treated with chlorhexidine (an in-vitro study).

Author: Aliaa M. Jabbar
Journal: Journal of baghdad college of dentistry مجلة كلية طب الاسنان بغداد ISSN: 16800087 Year: 2017 Volume: 29 Issue: 1 Pages: 16-19
Publisher: Baghdad University جامعة بغداد

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Abstract

Background: One of the challenges to use chlorhexidine is its effect on the amount of microleakage after restoration; however, use of the materials with antibacterial properties after tooth preparation and before restoration has been widespread. The objective of this, in-vitro, study was to evaluate the influence of consepsis (chlorhexidine gloconate disinfectant) application on microleakage in class II cavities restored with light cured composite using universal adhesive system; etch and rinse technique –self etch technique.Materials and Methods: Forty class II cavities were prepared on mesial and distal surfaces of 20 non-carious mandibular third molars. The cavities were divided into four groups; (n =10 for each group). G1: cavities were bonded with (single bond universal adhesive, 3MESPE) after etching with 35% phosphoric acid - etch and rinse technique, without chlorhexidine , G2: chlorhexidine was used after etching with 35% phosphoric acid, then cavities were bonded with (single bond universal adhesive, 3MESPE) - etch and rinse technique . G3: cavities were bonded with (single bond universal adhesive, 3MESPE) - self etch technique, without chlorhexidine, G4: The chlorhexidine was used before bonding. All groups were restored with non – hybrid composite resin (Z 350, 3MESPE). Micrlerackage was evaluated at gingival margin using methylene blue dye penetration method. In each cavity the dye penetration in millimeters was measured by strereomicroscope. Statistical analysis for the data was carried out using one-way ANOVA and LSD test.Results: Group G2 produced the least microleakage which is statistically highly significantly different than the remaining groups. Group G3 produced the highest microleakage which is statistically high significant difference than G1 and G2, but the difference is not significant with group G4.Conclusions: Consepsis can be used as cavity disinfectants when restoring the cavities with single bond universal adhesive as etch and rinse technique. However, its effect seems to be lower when using the single bond universal adhesive as self-etch technique.


Article
Comparative antiplaque activity of propolis extract and chlorhexidine in vivo

Authors: Baceer A Abdullah --- Rafi’ A Al–Talib --- Fa’iz A Al–Sultan
Journal: Al-Rafidain Dental Journal مجلة الرافدين لطب الأسنان ISSN: 18121217 Year: 2003 Volume: 3 Issue: 1 Pages: 7-12
Publisher: Mosul University جامعة الموصل

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Abstract

This study compared the clinical efficacy of different mouthwashes [0.2% chlorhexidine gluconate (CHX), 0.5%, 1% water extract of propolis, 0.5%, 1% ethanolic extract of propolis] with distilled water in their ability to inhibit plaque accumulation. In this double blind study, 10 (8 males and 2 females) dental students volunteers aged from 20-24 years, 1120 tooth surfaces from 280 teeth were examined. Each volunteer received a final professional tooth cleansing and was instructed to stop all mechanical tooth cleaning effort for next 5 days, where the mouthwashes used 3 times daily. Plaque system index (Silness and Löe, 1964) was obtained from teeth surfaces (buccal, lingual, mesial and distal) before and after the uses of mouthwashes. The results of this study showed significant difference (p < 0.05) between 0.2% CHX, 0.5% and 1% water extract of propolis, 0.5% ethanolic extract of propolis and distilled water, but non significant difference (p > 0.05) between CHX and 1% ethanolic extract of propolis in their ability to inhibit plaque accumulation. It can be concluded that the alcoholic extract of propolis may be used as adjunct to mechanical plaque control during the maintenance phase of therapy to ensure sustained low plaque level and this may meet patient approval because it is a natural substance and devoid of industrial chemical component.


Article
Effect of chlorhexidine mouth wash and flouridated mouth wash on mechanical properties of orthodontic arch wires (An in vitro study)

Authors: Khawla M Awni --- Hind T Jarjees --- Hakam H Sabah
Journal: Al-Rafidain Dental Journal مجلة الرافدين لطب الأسنان ISSN: 18121217 Year: 2011 Volume: 11 Issue: 3 Pages: 150-159
Publisher: Mosul University جامعة الموصل

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Abstract

Aims: The aims of the this study were to measure the effect of chlorhexidine and fluoridated mouth wash on mechanical properties of stainless steel and nickel titanium wires and to study the effect of three time intervals (3, 7, and 10 days) immersion on these mechanical properties. Materials and Methods: Two types of orthodontic wires were taken which included stainless steel and superelastic nickel titanium wires (Dentaurum, Germany). The 0.016×0.016 inch wires were selected. Each type of wires divided into seven groups; control group and six experimental groups in which the wires immersed in the chlorhexidine and fluoridated mouth wash for 3, 7, and 10 days and then the mechanical properties of wires (yield stress, ultimate tensile stress, modulus of elasticity) measured by using the universal tensile testing machine. Results: The results of the present study showed that a significant difference in the mechanical properties of both stainless steel and nickel titanium wires between the control group and experimental groups immersed in the fluoridated mouth wash and this decreased in the mechanical properties as immersion time increased. Also the results of the present study showed that a non significant difference in the mechanical properties of both stainless steel and nickel titanium wires between the control group and experimental groups immersed in the chlorhexidine mouth wash. Conclusions: The results of the present study indicated that the fluoridated mouth wash decreased the mechanical properties of stainless steel and nickel titanium wires and this degradation in mechanical properties could contributed to prolong orthodontic treatment. While the chlorhexidine has no effect on mechanical properties of stainless steel and nickel titanium wires


Article
The effect of different storage times and storage conditions of two types of alginate impression on accuracy of master cast

Authors: Najlan A JASIM --- Munther N KAZANJI
Journal: Al-Rafidain Dental Journal مجلة الرافدين لطب الأسنان ISSN: 18121217 Year: 2001 Volume: 1 Issue: 3 Pages: S363-S374
Publisher: Mosul University جامعة الموصل

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Abstract

The aims of this study were to determine the effect of using different storage time and storage media on the accuracy of two types of alginate impression material,Hydrogum soft and Hydrogum elastic.One hundred sixty impressions were recorded from a standard acrylic model using perforated trays. These impressions were divided into three main groups. In the first group,(48) impressions were stored at (3) times interval namely (15) minutes,(30) minutes,(60) minutes and left on the bench uncovered. In the second one,(48) impressions were stored in disposable plastic bags at the same time interval mentioned above. In the third one,(48) impressions were divided into (3) subgroups, the first one were only washed with tap water before pouring, the other two subgroups were immersed in Sodium Hypochiorite(0.5%) and ChlorhexidineGluconate(0.5%) disinfectant solutions for (30) minutes before pouring,(16) impression will be taken and poured immediately to be considered as a control for this study.A three dimensional measuring machine (3D) was used for measuring the dimensional change of stone casts, the results showed that there was no significant differences of casts accuracy produced from the two types of an alginate impression material.Storage of alginate impression material at (15) and (30) minutes on the bench or inside sealed plastic bags showed no significant differences in dimensional changes compared with control samples that poured immediately, Storage to (60) minutes showed a significant difference,washing alginate with tap water and immersion in chlorhexidine disinfectant solution (0.5%) for (30) minutes showed no significant difference Immersion in sodium hypochlorite (0.5%) for (30) minutes showed significant changes.It was concluded that alginate impression could be stored for (30) minutes inside bags without significant changes if the pouring of alginate impression will be delayed for any reason.


Article
The Microbial Contamination of Toothbrushes and Their Disinfection by Antimicrobial Solutions

Authors: Eman A Mustafa --- Rayia J Alnaimi --- Rafi A Al–Talib
Journal: Al-Rafidain Dental Journal مجلة الرافدين لطب الأسنان ISSN: 18121217 Year: 2008 Volume: 8 Issue: 2 Pages: 144-150
Publisher: Mosul University جامعة الموصل

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Abstract

Aims: To evaluate the bacterial survival rate on toothbrushes after brushing and the efficacy of their decontamination by immersing them in different antimicrobial solutions when toothbrushes were not inuse. Materials and Methods: Twenty healthy dental students participated in this study, they were all supplied with a new toothbrush of the same brand and type together with identical tubes of fluoridated toothpastes and were asked to brush twice daily for a period of 4 weeks during which they were asked to follow their oral hygiene practices. The students were divided into 3 groups, the first group kept theirtoothbrushes in a ventilated area exposed to air after brushing; the second group kept their toothbrushes soaked in 1% Sodium hypochlorite solution (1 part bleach, 4 parts of distilled water); the third groupimmersed their toothbrushes in 0.2% chlorhexidine gluconate solution. After one month the toothbrushes were collected, decapitated and examined in the laboratory by making bacteriological cultures to identify the aerobic and anaerobic microorganisms in each toothbrush, also the total live bacterial counts per toothbrush were obtained. Results: The toothbrushes that were not immersed in any antimicrobial solution were heavily contaminated and the immersing of toothbrushes in 0. 2 % chlorhexidine gluconate solution was a very effective method in reducing the mean number of aerobic and anaerobic microorganisms. Conclusions: Toothbrushes can become contaminated after approximately1 month of use, it is therefore recommended for individuals to use solutions like 0.2 % chlorhexidine gluconate which proved to be an effective antimicrobial agent to reduce toothbrush contamination.


Article
The staining effect of chlorhexidine mouthwash on non metallic brackets (An in vitro comparative study)

Authors: Hayder J. Attar حيدر جاسم العطار --- Fakhri A. Ali فخري عبد علي
Journal: Journal of baghdad college of dentistry مجلة كلية طب الاسنان بغداد ISSN: 16800087 Year: 2012 Volume: 24 Issue: 2 Pages: 109-113
Publisher: Baghdad University جامعة بغداد

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Abstract

Background: Since it is needed to have means other than mechanical plaque control to achieve good oral hygienein orthodontic patients, and since an eliminating the metallic appearance of orthodontic appliance is alwaysdesired to achieve a maximum esthetic appliance, so this study was done to investigate and compare the stainingeffects of chlorhexidine mouthwash 0.2% on the un bonded ceramic brackets, ceramic brackets bonded with nomix adhesive, ceramic brackets bonded with light cured adhesive, un bonded composite brackets, compositebrackets bonded with no mix adhesive and composite brackets bonded with light cured adhesive.Materials and Methods: The effect of the chlorhexidine was studied through immersion the brackets and bondedbrackets in the mouth wash for three different time intervals: 1, 2 and 3 hours, which represent the accumulated dailyuse of the mouthwash for 1, 2, and 3 months respectively and compared them with corresponding control groupswhich not immersed in chlorhexidine 0.2%. The sample consisted of two hundred eighty eight brackets. AShimadzu,UV 160A UV-Visible spectrophotometer was used to perform a light absorption test for each subgroup with twelvebrackets each.Results: ANOVA and LSD post Hoc tests were used to identify the significant effects of the mouthwash at asignificance level P . 0.05, A. significant effects identified with ceramic brackets bonded with no mix adhesive,ceramic brackets bonded with light cured adhesive, un bonded composite brackets, composite brackets bondedwith no mix adhesive and composite brackets bonded with light cured adhesive, while non significant effect of unbonded ceramic brackets.Conclusions: It can be concluded that the chlorhexidine mouthwash do not have a staining effect on the unbonded ceramic brackets while significant changes in staining effect when ceramic and composite bracketsbonded to no mix adhesives and that effect decrease when bonded to light cured adhesives. The mouthwash has astaining effect on the un bonded composite brackets also.


Article
Effect of ginger extract on Mutans streptococci in comparison to chlorhexidine gluconate

Authors: Tuqa Akram Weli تقى اكرم --- Ahlam Taha Mohammed احلام طه محمد
Journal: Journal of baghdad college of dentistry مجلة كلية طب الاسنان بغداد ISSN: 16800087 Year: 2013 Volume: 25 Issue: 2 Pages: 179-184
Publisher: Baghdad University جامعة بغداد

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Abstract

Background: The rhizome of ginger is used in cooking and for medicinal purposes such as anti-bacterial, anti-fungal,anti-inflammatory and antioxidant. The aims of the study were to test the effect of ethanolic extract of ginger ongrowth, adherence and acidogenicity of mutans streptococci in comparison to chlorhexidine gluconate 0.2% andde-ionized water.Materials and methods: From saliva often volunteers (dental students 20-22 years); mutans streptococci was isolated,purified and diagnosed according to morphological characteristic and biochemical tests. Ginger was powderedand extracted, different concentrations of ginger extract were prepared. Chlorhexidine gluconate 0.2% used as acontrol positive; while de-ionized water was used as a control negative. In this study, in vitro and in vivo experimentswere conducted. In vitro experiment, agar well technique was used to study the sensitivity of mutans streptococci todifferent concentrations of ginger extract and other control agents; also effect of ginger extract on the viable countof mutans streptococci, the adherence and acidogenicity of mutans streptococci were studied.In vivo experiment,the volunteers couldn’t tolerate the extract.Results: Mutans streptococci was sensitive to different concentrations of ethanolic ginger extract, but they weremore sensitive to chlorhexidine gluconate than the extract. The effect of ginger extract on the viable count ofmutans streptococci at concentrations (30%, 35% and 40%) showed highly significant reduction in the count of thebacteria but less than chlorhexidine effect. In the effect of the extract on the adherence of mutans streptococci, theconcentrations (30%, 35%, 40%) were used and only 40% and chlorhexidine prevent the plaque formation. But in theacidogenicity of mutans streptococci procedure 35%, 40% of the extract and chlorhexidine showed effectiveness inreducing acid formation.Conclusion: Ginger extract was effective against mutans streptococci, chlorhexidine is more effective than otheragents.

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