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Article
The Prognostic Role of (CD34) In The Angiogenesis of Endometrial Adenocarcinoma

Author: Lubab Fadhil Talal
Journal: Iraqi Academic Scientific Journal المجلة العراقية للاختصاصات الطبية ISSN: 16088360 Year: 2006 Volume: 5 Issue: 1 Pages: 77-86
Publisher: The Iraqi Borad for Medical Specialization المجلس العراقي للاختصاصات الطبية

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Abstract

ABSTRACT: BACKGROUND: Tumor angiogenesis is essential for tumor growth and metastases. Recently ,studies showed that microvessel density (MVD),a measure of tumor angiogenesis has found to have a prognostic significance for predicting metastases and survival in endometrial adenocarcinoma and identifying patients at high risk for recurrences . The aim of this study is to understand the correlation between the MVD and various clinicopathological factors in endometrial adenocarcinoma in order to evaluate the role of angiogenesis in the assessment of the biological behavior of endometrial adenocarcinoma and to prove that MVD can be used as a valuable prognostic factor and can detect high risk patients for post-operative recurrence who may need adjuvant therapy after surgery . PATIENTS AND METHODS: Forty-one (41) cases were included in this study ; one case is normal endometrium in the proliferative phase , 5 cases with sever endometrial hyperplasia and 35 cases with endometrial adenocarcinoma taken from the Pathology Department at the Medical City and Private Pathology Laboratory between the years 2000-2004 . Paraffin blocks cut in two sections ; one for Haematoxylin & Eosin stain and the other for CD34 immunostaining slides were studied well for tumor grade ,stage ,myometrial invasion and pelvic and paraaortic lymph nodes metastases . The mean MVD was studied by scanning the tumor sections at a low power X40 and X100 then identifying areas of highest neovascularization (hot spots) .Five hot spot areas were selected from each section ,and the MVD were counted at X200 magnification (x10 ocular ,x20 objective) by taking the mean of those microvessels. The correlation between mean MVD and various clinicopathological factors were studied including the age ,tumor grade ,stage ,myometrial invasion and pelvic and paraaortic lymph nodes metastases . RESULTS : CD34 is a reliable and sensitive endothelial cell marker used in the assessment of tumor angiogenesis The study showed that a significant statistical correlation was found between the mean MVD and patients age , tumor grade and myometrial invasion. While there were no statistical correlation significance between the mean MVD and tumor stage and pelvic & paraaortic lymph nodes . CONCLUSION : We conclude that MVD is important in the assessment of the biological behavior of endometrial adenocarcinoma , where it is an indicator for the emergence and growth of tumor and it is important to detect patients at high risk of recurrences and to decide the post-operative adjuvant therapy


Article
Evaluation of Immunohistochemical Staining for Hepatocyte Growth Factor and c-Met in Endometrial Adenocarcinoma

Authors: Lubab Fadhil Talal --- Mohamed Sabaa Challob
Journal: Iraqi Academic Scientific Journal المجلة العراقية للاختصاصات الطبية ISSN: 16088360 Year: 2013 Volume: 12 Issue: 3 Pages: 335-342
Publisher: The Iraqi Borad for Medical Specialization المجلس العراقي للاختصاصات الطبية

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Abstract

ABSTRACT: BACKGROUND:Endometrial carcinoma is a primary malignant epithelial tumor, usually with glandular differentiation,arising in the endometrium with the potential to invade and metastasize. Hepatocyte growth factor(HGF) and its receptor c- Met have been implicated in uterine development, pregnancy, andendometrial disorders, such as endometriosis and carcinoma. OBJECTIVE:The goal of this study was to evaluate immunohistochemical (IHC) staining patterns of HGF and cMetinendometrialadenocarcinomaandtocorrelatestainingwiththebiologicalbehaviorandoutcomeofendometrialadenocarcinoma.PATIENTSANDMETHOD:Aretrospective study included 45 cases ,who underwent total abdominal hysterectomy and bilateralsalpingo-oopherectomy, between 2005-2010 samples ,were taken from Teaching Laboratories atBaghdad Teaching Hospital/Medical City. Thirty cases were diagnosed as adenocarcinoma of the endometrium, an additional 15 patientsdiagnosed as to have uterine leiomyoma(fibroid) and who had normal endometrium were taken as acontrol group. The patient's age ,tumor grading ,depth of myometrial invasion, presence of pelvic andparaaortic LN metstases, vascular invasion ,and the stage of the disease were noted .The specimens were already fixed in10% formalin , and paraffin embedded. Three sections (4 micron inthickness ) were cut from each paraffin block. One section stained with Haematoxylin and Eosin(H&E) stain , and the other two stained with HGF and c-Met immunostaining antibodies usingpositively charged slides . RESULTS: The mean age of the patients with endometrial adenocarcinoma was 58.5years. There was no statistically significant difference between HGF/c-Met expression and age, tumor grade,stage , myometrial invasion and vascular invasion. There was a statistical significant correlationbetween HGF and c-Met scores with cases showing no pelvic lymph nodes metastases. There is a linear increase in HGF and c-Met expression in both the diseased and control group.Astatistical significant correlation was found in HGF and c-Met scores between the diseased group andthe control group.CONCLUSION:HGF and c-Met staining was significantly different between control group and diseased group. HGF and c-Met Showed Linear increase Expression in both diseased and control group .The c-Met is the high-affinity receptor for hepatocyte growth factor


Article
Role of SOX4 gene in the progression of endometrial hyperplasia to endometrial adenocarcinoma

Author: Mahdi Saber Al-Deresawi 1 , Abdul Hussein Moyet AlFaisal 2 , Salim Rasheed Al- obaidi 3
Journal: Iraqi Journal of Biotechnology المجلة العراقية للتقانات الحياتية ISSN: 18154794 Year: 2018 Volume: 17 Issue: 2 Pages: 82-90
Publisher: Baghdad University جامعة بغداد

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Abstract

The objective of this study was to investigate the role of SOX4 gene in progression of endometrial hyperplasia to endometrial adenocarcinoma .A total of 41 Iraqi patients with abnormal uterine bleeding followed by hysterectomy. Histological findings recorded 36 cases with endometrial hyperplasia and 5 endometrium adenocarcenoma. Cases collected during the period from February 2017 to October 2017. Curettage techniques were used to obtain a ten samples as healthy control group. All cases were obtained from Al-Zahra Teaching Hospital in Wasit Province . Endometrial hyperplasia subjects distributed according to age, the highest percentage ranged (40-50)years recorded (58.33%). To evaluate the SOX4 gene expression, total RNAs were extracted with TRIzol from each sample then directly converted to cDNA. RT-PCR technique was used to estimate the SOX4 gene expression and (GAPDH gene used as a reference gene). The results revealed that there was a highly significant increase (p<0.01) in SOX4 gene expression in endometrium adenocarcenoma (9.24±0.52) followed by endometrial hyperplasia (6.14±0.17) when compared to the healthy control group (1±0.00).In conclusion, endometrial hyperplasia can be a precursor of endometrium adenocarcenoma when the SOX4 gene expression elevated gradually.


Article
Relationship Determination betweenHeartDiseases with Endometrial Adenocarcinoma and Ovarian Carcinoma by Evaluating the HPV16 IgG , IgM and p53 in Some IraqiFemale Patients

Author: Jasim Mohammed Muhsin
Journal: Iraqi Journal of Biotechnology المجلة العراقية للتقانات الحياتية ISSN: 18154794 Year: 2016 Volume: 15 Issue: 2 Pages: 74-80
Publisher: Baghdad University جامعة بغداد

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Abstract

Heart diseases problems linked to female genitalia associated carcinomas were considered as a major issues and life threat; therefore, it was necessary to determine the relationship between heart diseases problems with HPV16 infection and P53 expression in some groups of female Iraqi patients that suffer from endometrial Adenocarcinoma (EC) and ovarian carcinoma. This study was designed as a two prospective groups. It involved ninety samples of serum were collected from the Medical City of Baghdad from the period of October 2014 to January 2015 as 45 50% of endometrial Adenocarcinoma (EC) patients group and 45 (50%) of ovarian carcinoma patients group then stored in -80°C. All samples were examined for HPV16 IgG, IgM and p53 expression by the enzyme-linked immunosorbent assay (ELISA).Highly significant difference (p <0.001) of Mutant p53 expression has been detected in 32 35.6% out of 41 45.6% patients with heart diseases history in comparing with patients without history of heart diseases. In addition to , highly significant differences (p < 0.001) of HPV16 IgM has been detected in 36 patients had previous history of heart problems as 40% out of 41 patients. In the other hand, Endometrial Adenocarcinoma (EC) cases, have been a statistically significant differences with HPV16 IgG in 35 38.9 % and HPV16 IgM in 31 34.4% positive results out of 45 cases (p < 0.05 ) with highly significant differences in the positive cases of 32 35.6% that had been detected with heart diseases problems (p < 0.001). Furthermore, the ovarian carcinoma cases have positives HPV16 IgG and HPV16 IgM levels as 24 26.7 % and 18 20% respectively with significant difference regarding to the negative results (p < 0.05 ).An interesting results has been detected recently in this study, as HPV16 infection followed by p53 inactivation may initiate heart diseases in some groups of females Iraqi patients that had been with endometrial Adenocarcinoma or ovarian carcinomas. So that, HPV16 infection plays a major role in the heart diseases progression through it E6 and E7 oncoproteins to the sabotage of the normal p53 and pRb pathways.

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