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In-vitro Comparative Study of Antibacterial activity of Syzygium aromaticum with three Antibiotics Against E. coli
دراسة مقارنة مختبرية للفعالية المضادة للبكتريا للقرنفل مع ثلاثة انواع من المضادات الحياتية ضد بكتريا الاشيريكيا كولاي

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Abstract

The results confirmed antibacterial activity with significant differences (P≤0.05) of clove (Syzygium aromaticum) in the concentration 10µg/ml(MIC) (1.10+ 0.31). Also superiority of the antibiotic (Doxycycline) that has MIC (Minimum Inhibitory Concentration) at 10 µg/ml by significant differences (p≤0.05) (2.00+0.08) aginst E . coli that appears sensitive to its, while it doesn’t clear any significant differences in both of the antibiotics (Gentamicin and Gentadoxycycline). In case antibacterial activity of antibiotic (Gentamicin) against E . coli by sensitive tests observe resistance of E . coli , started from 10 µg/ml (1.60+0.15) to 100 µg/ml (2.30+0.05) by significant differences (p≤0.05). Also the antibiotic (Gentadoxycycline) by combination of (Gentamicin and Doxycycline) in-vitro ,it doesn’t show any significant differences (p≤0.05) when applicated the sensitive tests of the antibiotic against E . coli at concentration 10 µg/ml (1.80+ 0.15) and 100 µg/ml (2.60+ 0.19), which demonstrates antagonist between them; by numbering of inhibition zone appeard that the results less than (Doxycycline) and more than (Gentamicin), As a whole, these results illustrate superiority of Doxycycline as a antibacterial activity against E . coli depended on sensitivity tests according to antibiotics(Gentamicin and Gentadoxycycline) and clove(Syzygium aromaticum) by significant differences (p≤0.05) .Objective Knowing of antibacterial activity of clove (Syzygium aromaticum) against bacteria (Escherichia coli ) in in-vitro, and compare of it with three antibiotics (Gentamicin, doxycycline and Gentadoxycycline), by measurement of inhibition zone for both of them.Material and Methods Bacteria (E . coli ) was isolated from the patients in the city of al- Sadir medical on E.M.B (Eosin Methylene Blue) agar and incubated at 37 o C for 18-24 hours. This trial is performed in in-vitro (sensitivity test) by agar well diffusion technique, in diameter of well (8 mm) and by measurement of inhibition zone in nutrient agar after 24 hrs, and knowing of Minimum Inhibitory Concentration (MIC) for both of them. Conclusions Syzygium aromaticum has high antibacterial activity against the Bacterial growth of (Escherichia coli). Doxycycline has high antibacterial activity against the Bacterial growth of (Escherichia coli). Resistance of E.coli to the commonly used eradication antibiotic drug(Gentamicin). The combination between two antibiotics (Gentamicin and doxycycline ) doesn’t give antibacterial activity against the Bacterial growth of (Escherichia coli).

أكدت النتائج بأن للقرنفل (Syzygium aromaticum ) فعالية ضد بكتريا الـ (E . coli ) وبفروقات معنوية (p≤0.05) . وبينت النتائج تفوق المضاد الحيوي (Doxycycline) بالمقارنة مع بقية المضادات الحياتية (Gentadoxycycline و Gentamicin ) والقرنفل (Syzygium aromaticum ) بتركيز (10 µg/ml) (2.30+0.05) MIC . لم تظهر أي فروقات معنوية (p≤0.05)عند خلط مضادين حياتيين في المختبر(Doxycycline و Gentamicin ) من خلال قراءة منطقة التثبيط وعند تركيز (10 µg/ml) (1.80+0.15) وهي اقل من الـ (Doxycycline) و أكثر من الـ(Gentamicin ). الهدف : معرفة الفعل المضاد البكتيري للقرنفل (Syzygium aromaticum ) ضد بكتريا الـ (Escherichia coli ), ومقارنتها مع ثلاثة انواع من المضادات الحياتية (Gentamicin, doxycycline and Gentadoxycycline) ٬ من خلال قياس منطقة التثبيط لكل واحد منهم مختبريا . طريقة العمل : عزلت بكتريا الـ(E . coli ) من مدينة الصدر الطبية على وسط انتخابي (E.M.B) agar (Eosin Methylene Blue) agar ووضعت في الحاضنة عند درجة 37 م0 للفترة 24 ساعة, وبعد ذلك أ ًجريت لها فحوصات حساسية لكل من (Syzygium aromaticum, Gentamicin, doxycycline and Gentadoxycycline) بتقنية انتشار في الحفر في وسط هلام الاغار, وقطر الحفرة (8 mm) , ومعرفة اوطأ تركيز مثبط لنمو البكتريا من خلال قياس منطقة التثبيط لكل منهم في الوسط المغذي بعد 24 ساعة من الحضانة.

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