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Antiviral Effect of Ethanolic Extract of Salvadora Persica (Siwak) on Herpes Simplex Virus Infection.

Author: Mahmoud YM Taha
Journal: Al-Rafidain Dental Journal مجلة الرافدين لطب الأسنان ISSN: 18121217 Year: 2008 Volume: 8 Issue: 1 Pages: 50-55
Publisher: Mosul University جامعة الموصل


Aims: To investigate the effect of ethanolic extract of Salvadora Persica extract on HSV–1 infection
both in vitro and in vivo in the mouse model system. Materials and methods: Ethanolic extract of
Salvadora Persica was used at different concentrations. BHK cells that grown in Eagles medium were
used for virus isolation and titration using PFU/ml. The effects of different concentrations of Salvadora
Persica on viral growth in BHK cells as well as cytolytic activity of HSV–1 were evaluated at different
time post infection. The therapeutic efficacy of Salvadora Persica in vivo was studied in mice. Lesions
were scored and viral isolation from infected skin and ganglia was titrated on BHK cells. Results:
Salvadora Persica inhibited the replication of HSV–1 in BHK cells as well as the cytolytic activity of
cell free virus. Topical application of Salvadora Persica on the skin of mice infected with HSV–1
reduced the development of cutaneous lesions and the viral titers in the skin and ganglia were also
reduced. Conclusion: The results of this work may be beneficial for the treatment of recurrent oral
herpes infections.


Miswak --- Antiviral --- HSV–1

Relationship between Herpes simplex Virus Type-1 and Candida albicans in Pregnant Women with Aphthous Stomatitis in the oral cavity

Authors: Mohammad T. Abdul Hussin محمد عبد الحسين --- Sana A. AL-Shaikhly سناء الشيخلي
Journal: Journal of baghdad college of dentistry مجلة كلية طب الاسنان بغداد ISSN: 16800087 Year: 2012 Volume: 24 Issue: special issue 1 Pages: 158-163
Publisher: Baghdad University جامعة بغداد


Background: This study was aimed to show the relationship between Herpes simplex virus type-1 (HSV-1) andCandida albicans, and to determine HSV-1 antigen in saliva of aphthous stomatitis patients by immunoflourescent(IF), as well as to determine HSV-1 antibodies immunoglobulin G (IgG) in saliva samples by enzyme linkedimmunosorbent assay(ELISA)test ,in addition to study concentration of the total salivary protein.Materials and methods: Sixty pregnant women with aphthae and thirty healthy control subjects were included in thestudy. Saliva samples were taken from all the subjects and examined by direct IF and ELISA, and using the salivaswab samples for isolation of Candida albicans .The isolated colonies were identified by germ tube formation,growth at 45 oC, and measuring the total salivary protein.Results: The results of the present study showed that aphthous were most prevalent at age group(26-30) years. Themost commonly affected mucosa are the labial mucosa, buccal mucosa, tongue, and rarely the soft palate andfloor of the mouth. Positive IF results were observed in 53.33% in patients and 6.67% in healthy control, while in ELISA,the positive results were found to be 63.33% in patients and 6.67% in control subjects. There was significant differencebetween anti HSV-1 IgG Abs and total salivary protein.Conclusion: the present study show there was no difference between Candida albicans and HSV-1 .The presentstudy indicated that HSV-1 was detected serologically in saliva of patients by ELISA and IF method .The resultsrevealed positive association between HSV-1 and aphthous, and the virus may play a role in the occurrence of theaphthous. Saliva is regarded as a transudate of the serum and it contains the same antibodies as serum, and asimilar range of IgG antibodies, but at a much lower concentration and it's easily available and simply examined.The total salivary protein may play a role in the defense against the virus


HSV-1 --- aphthous --- ELISA --- IF

Seroprevalence of Herpes Simplex Virus type-1antibodies (IgM,IgG) in smokers in Kirkuk city-Iraq
انتشار اضداد فايروس الهيربس البسيط_ النوع الاول (IgM (IgG,في المدخنين في محافظة كركوك- العراق

Author: Lezan Medhat Mohammed Zangana ليـزان مـدحت محمد زنكنة
Journal: Tikrit Journal of Pure Science مجلة تكريت للعلوم الصرفة ISSN: 18131662 Year: 2016 Volume: 21 Issue: 1 Pages: 36-40
Publisher: Tikrit University جامعة تكريت


Herpes Simplex Virus type-1 (HSV-1) is classified in the α-herpes virus group of the Herpesviridae and HSV-1 infections are very common in the human population. The objective of this study is to determine the prevalence of anti-herpes simplex virus type-1 IgM and anti-herpes simplex virus type-1 IgG among smokers and nonsmokers. In the current study, A total of one hundred two serum samples from males were collected (70 smokers and 32 nonsmokers), there ages were ranged between 20- 50 year; this study which was done during the period between September 2014 to the January 2015. All obtained sera samples from all persons were tested using ELISA method for determine of Herpes simplex virus type-1 antibodies. Results in this study showed that the herpes simplex virus type-1 IgM seropositivity among smoker persons was 24 (34.3%). In addition, the result revealed that the percentage rate of the herpes simplex virus type-1 IgG among smokers was 61(87.1%). There was a significant effect of smoking on the seroprevalence of Herpes simplex virus type-1 antibodies (IgM , IgG).

فايروس الهيربس البسيط النوع الاول مصنف من مجموعة الفا هيربس فايروس وعائلة هيربس فيريداى. ان اصابات فايروس الهيربس البسيط النوع الاول شائع جدا في الانسان. ان هدف هذه الدراسة هو تقـديـر اضـداد فايروس الهيربس البسيط_النوع الاول( IgM ,IgG ) ايجابية المصل في المدخنين و غير المدخنين. في الدراسة الحالية جمعت مائة واثنان عينة مصل من الذكـور (70 مدخنين و 32 غير مدخنين ) وقـد تراوحت اعمارهـم بين (20-50) سنة, اجريت هـذه الدراسـة في محافظة كركوك للفترة من شهر ايلول/2014 ولغـايــة شهر كانون الثاني/2015 . جميع المصول التي تم الحصول عليها من جميع الاشخاص المشمولين بالدراسة تم اختبارها باستخدام طريقة ال ELISA لتحديد الاجسام المضادة لفايروس الهيربس البسيط_ االنوع الاول. بينت االنتائج في الدراسة الحالية ان نسبة انتشار اضـداد فايروس الهيربس البسيط_االنوع الاول)IgM( في المدخنين كان موجبا بنسبة( 24 (34.3% , وان نسبة انتشار اضـداد فايروس الهيربس البسيط_االنوع الاولIgG) ) في المدخنين كان موجبا بنسبة 61(87.1%). حيث بينت الدراسة ايضا انه توجد دلالة معنوية في تاثير التدخين على انتشار اضداد فايروس الهيربس االبسيط_النوع الاول( IgM ,IgG ).

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