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Article
4.SERUM LEVELS OF GLYCODELIN A AND SOLUBLE INTRACELLULAR ADHESION MOLECULE-1 AS BIOMARKERS FOR ENDOMETRIOSIS

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Abstract

Background:Endometriosis is a benign chronic disease, characterized by the presence and proliferation of functional endometrial gland and stroma outside the uterine cavity.Objective: To evaluate the usefulness of intracellular adhesion molecule-1 (ICAM-1) and Glycodelin (Gd) as a biomarker for the diagnosis of endometriosis and to help in detection of various stages of endometriosis.Methods: Forty-four patients with endometriosis and 35 apparently healthy women as control were enrolled in this study from November 2015 to April 2016. All individuals were subjected to blood sampling for measuring their serum ICAM-1 and Gd A level by using enzyme linked immune sorbent assay technique. Results: The current study revealed significantly higher serum levels of ICAM-1 and Gd in patients group in comparison with healthy control. In addition, the sensitivity and specificity for ICAM-1 and Gd A in serum were 61%/ 66%, 76%/ 85% respectively.Conclusion: The ICAM-1 and Gd A level in serum may be useful as noninvasive test for diagnosis of endometriosis in all stages.Keywords: Endometriosis, ICAM-1, GlycodelinCitation: Draj HA, Abbas AA, Abdullah TH. Serum levels of Glycodelin A and Soluble Intracellular Adhesion Molecule-1 as biomarkers for endometriosis. Iraqi JMS. 2017; Vol. 15(4): 345-349. doi: 10.22578/IJMS.15.4.4

Keywords

Endometriosis --- ICAM-1 --- Glycodelin


Article
Seroprevalence of Rhinovirus in Common Cold Patients in Relation with ICAM-1 Level in Tikrit City
الانتشار المصلي لفيروسات الأنف في مرضى البرد الشائع في علاقة بمستوى ICAM-1 في مدينة تكريت

Authors: Thekra A. Hamada ذكرى حمادة --- Israa Ali Arif اسراء علي
Journal: Al-Kufa University Journal for Biology مجلة جامعة الكوفة لعلوم الحياة ISSN: 20738854 23116544 Year: 2019 Volume: 11 Issue: 2 Pages: 61-66
Publisher: University of Kufa جامعة الكوفة

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Abstract

Human rhinoviruses is the major cause of cold illness, also this virus related with more severe illness like exacerbation of asthma, chronic obstructive pulmonary disease and the most causative agents of upper respiratory tract complications. The study aims to evaluate the relation of ICAM-1 levels in HRV infection among common cold patients. Across sectional study was carried out in Salahaldin governorate from December, 2017 to March 2018. The number of patients were 70 patients who clinically infected with common cold and were 17-66 years old that belonged different geographical area of Salahaldin governorate. The control group were 20 healthy individuals who matching the patients and apparently haven’t any diseases. Sera from patients and control were obtained for estimation of anti-HRV IgA and ICAM-1 by ALISA technique.

فيروسات الأنف البشرية هي السبب الرئيسي لمرض البرد ، كما يرتبط هذا الفيروس بمرض أكثر حدة مثل تفاقم الربو ومرض الانسداد الرئوي المزمن والعوامل المسببة لمضاعفات الجهاز التنفسي العلوي. تهدف الدراسة إلى تقييم العلاقة بين مستويات ICAM-1 في عدوى الهريفي لدى مرضى البرد الشائعين. أجريت دراسة مقطعية في محافظة صلاح الدين في الفترة من ديسمبر 2017 إلى مارس 2018. وكان عدد المرضى 70 مريضًا مصابين بالزكام ومرض الزكام وكان من 17 إلى 66 عامًا ينتمون إلى منطقة جغرافية مختلفة من محافظة صلاح الدين. كانت المجموعة الضابطة 20 من الأفراد الأصحاء الذين يتطابقون مع المرضى وعلى ما يبدو ليس لديهم أي أمراض. تم الحصول على الأمصال من المرضى والسيطرة لتقدير مكافحة الهريمات IgA و ICAM-1 بواسطة تقنية ALISA.


Article
Cardioprotective effect of tetrahydroisoquinoline following I/R through down-regulation of ICAM-1 signaling pathway

Author: Bassim I Mohammad
Journal: Muthanna Medical Journal مجلة المثنى الطبية ISSN: 2226146x Year: 2016 Volume: 3 Issue: 2 Pages: 122-132
Publisher: Al-Muthanna University جامعة المثنى

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Abstract

Myocardial injury caused by ischemia followed by reperfusion mediates a complex series of inflammatory response that reduces the benefit of medical interventions, such as percutaneous coronary intervention, and coronary bypass surgery. The objective of this study was to investigate the potential cardioprotective effect of tetrahydroisoquinoline against myocardial I/R, and the mechanistic pathway of this effect. A total of 32 adult (4 – 6 months) male Albino-Webster mice were randomly divided into 4 equal groups: (1) sham-control group, (2) ischemia and reperfusion (I/R) operated group, (3) vehicle-treated group, and (4) tetrahydroisoquinoline (TIQ)-treated group receiving TIQ 10 mg/kg once daily shortly before I/R. ELISA technique was performed to measure myocardial and plasma levels of cytokines (IL-1β, IL-6, and TNF-α), chemokine (MCP-1), and cTn-I. In addition, the activity of ICAM-1 was analyzed by Western blot. Further, the ischemia changes and myocytes injury were examined by histopathological assessment using hematoxylin and eosin (H&E) stain. The results demonstrated that treatment with TIQ markedly improved left ventricular function (LVF) in mice, and reduced plasma level of cTn-I as marker of cardiac injury. Moreover, the effects of TIQ was associated with attenuations in both chemokine and cytokines expression following I/R, that accompanied by down-regulation of activation of ICAM-1 pathway. In conclusions, this study revealed that treatment with TIQ was able to improve LV function after I/R. This improvement was associated with reductions of inflammatory response and activity of ICAM-1, as mechanistic link of its action.


Article
Immunohistochemical localization of intercellular cell adhesion molecule (ICAM-1) & vascular cell adhesion molecule (VCAM-1) in Radicular cyst

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Abstract

Objectives: Odontogenic cysts are one of the commonest bone destroying lesions of the maxillofacial skeleton, with the inflammatory radicular cyst being the most common jaw cyst.Cell adhesion molecules are known to be essential mediators of white blood cell adhesion & extravasations during inflammatory & immune reaction.Materials & Methods: Twelve formalin-fixed, paraffin-embedded radicular cysts were studied using LSAB/HRP immunohistochemical technique.Results: ICAM-1 expression was significantly high in radicular cysts with high expression in inflammatory cells infiltrate & connective tissue cells in cyst wall in comparison to VCAM-1 expression.Conclusion: In conclusion, the identification of the nature of adhesion molecules & inflammatory mediators involved not only expanded our knowledge of the molecular mechanisms but also provided the basis for a new understanding of the inflammatory response & its role in tissue homeostasis.


Article
Immunohistochemical detection of the expression of the cell adhesion molecules ICAM-1 & VCAM-1 in Pyogenic granuloma

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Abstract

Pyogenic granuloma is a relatively common benign vascular lesion of the oralmucosa & skin whose exact cause is unknown. The study of the expression of celladhesion molecules (ICAM-1 & VCAM-1) in oral pyogenic granuloma wasperformed.Fifteen formalin- fixed, paraffin-embedded oral pyogenic granuloma were studiedusing LSAB/HRP immunohistochemical technique.Mild to moderate expressions of cell adhesion molecules was demonstrated amongdifferent cell types of pyogenic granuloma suggesting that inflammatory response ofgingival tissue against oral antigens (gram negative bacteria) may play a major role ininflammatory neovasculairization & hence pathogenesis of pyogenic granuloma .


Article
The study of neuroprotective and anti-inflammatory effects of magnesium sulfate in rats following cerebral ischemia reperfusion injury
دراسة التأثير الوقائي الدماغي و المضاد للالتهاب لدواء كبريتات المغنيسيوم في الجرذان بعد نقص التروية الدموية الدماغية و اعادة الارواء

Author: Ahmed MH Al-Mudhafar أحمد محمد حسن المظفر
Journal: Kufa Journal For Veterinary Medical Sciences مجلة الكوفة للعلوم الطبية البيطرية ISSN: 20779798 Year: 2014 Volume: 5 Issue: 1 Pages: 1-9
Publisher: University of Kufa جامعة الكوفة

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Abstract

Background and objective: Magnesium sulfate has neuroprotective effects and decrease overall neuronal firing. It is also decrease firing of excitable tissues outside the brain. It is not known whether this neuroprotective effect is due to antioxidant effect, anti-inflammatory or other mechanism. In this research we study the anti-inflammatory effect of magnesium sulfate in rat brain following ischemia reperfusion stress.Material and methods: Twenty four rats were grouped into 4 groups: The first (sham group), the second (control) and the third group(control-vehicle) and the forth (treated with Magnesium sulfate). Animals in the second group underwent bilateral common carotid artery ligation without treatment, whereas the forth group were injected with magnesium sulfate 250mg/kg intraperitoneally before procedure. Blood samples were taken after the procedure for measurement of serum level of IL-9, MCP-1 and ICAM.Results: Serum level of IL-9 in control group was 163.3 ± 30.4 pg/mL and it significantly decreased in magnesium sulfate treated group (21.8 ± 1.72 pg/mL). serum level of MCP-1 in the control group was 109.05 ± 18.2 pg/mL while it significantly reduced in magnesium sulfate treated group (38.16 ± 3.54 pg/mL). mean serum levels of ICAM of control was 362.8 ± 26.81 pg/mL while mean serum level of ICAM in treated group was 35.5 ± 4.71 pg/mL.Conclusion: magnesium sulfate significantly decreases the inflammatory markers IL-9, MCP-1 and ICAM in global ischemia model in rats.

ان الإصابة بنقص التروية الدماغية وإعادة التروية (IRI) هي من العمليات المعقدة التي تؤدي إلى تلف الخلايا ثم موتها كما ان نقص التروية وأعادة الأرواء في الدماغ، كما هو الحال في الأجهزة الأخرى، يحفز استجابة التهابية والتي بدورها قد تؤدي إلى تفاقم مستويات الأولية لإصابات الأنسجة. أجريت هذه الدراسة لغرض التحقق من امكانية كبريتات المغنيسيوم في حماية الأعصاب و تحسين حال الإصابة الدماغية الشاملة (IRI) في نموذج الجرذان . تم اسخدام اربعة وعشرون جرذاً بالغاً استخدمت في هذه الدراسة ,وقد تم توزيعهم بشكل عشوائي الى اربعة مجاميع. أظهرت نتائج المستويات المصلية لكل من IL-9, وMCP-1 ICAM-1 زيادة احصائية (p<0.05) في مجموعة السيطرة عند المقارنة بمجموعة التظاهر. كما أظهرت المعالجة بكبريتات المغنيسيوم تأثيرا احصائيا (p<0.05)على الالتهاب من خلال تثبيط زيادة المستويات المصلية لعوامل الألتهاب IL-9,MCP-1,ICAM-1.


Article
Study ABO/Rh system with Endothelial Inflammatory Factors in Iraqi Arab Female with Diabetes Mellitus Type II
دراسة نظام ABO/Rh مع العوامل الألتهابية لبطانة الأوعية الدموية في مرضى السكري من النوع الثاني لدى النساء العراقيات العربيات

Authors: Rajaa M. Kaduhm رجاء مسلم كاظم --- Isam N. Salman عصام نوري سلمان --- Asmaa M. Salih أسماء محمد صالح
Journal: Baghdad Science Journal مجلة بغداد للعلوم ISSN: 20788665 24117986 Year: 2014 Volume: 11 Issue: 1 Pages: 93-102
Publisher: Baghdad University جامعة بغداد

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Abstract

Diabetes mellitus type II is a disorder of metabolism and complex diseases affected by genetic environmental factors and associated with inflammation. The symptoms of type II diabetes develop gradually, which are associated with increased blood concentration of marker of the endothelial inflammatory factors. The expression of adhesion molecules, including E-selectin, intracellular adhesion molecule-1(ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1) on the surface of vascular endothelial cells to help leukocyte stick to other surrounding tissues. Many researchers have made attempts to determine the significance of particular ABO phenotype for the susceptibility to diseases. Many reports show a strong association with the ABO blood groups and diabetes Type II. Dysfunction increases risk of type II diabetes among women with low level of subclinical incident diabetes. For that reason the present study has been designed to study the association between diabetic, endothelial dysfunction and blood group in Iraqi Arab diabetic women. Sixty patients of Iraqi Arabs female with previous diagnosed diabetic type II involved in this study and comparison with twenty controls matched in age, sex and ethnic groups. Both of patients and control divided into four sub groups according to the blood types. We evaluated endothelial inflammatory factors ICAM, VCAM and E-Selectin. Present data showed a significant difference in the serum level of ICAM-1 and E-section between diabetes mellitus type II patients and controls while there were no significant differences in the serum level of VCAM-1. In conclusion, significant increasing of the level of ICAM-1 and E-selectin made them as a risk factors to predict diabetes progression, women who carry blood group A, B and AB show appositive association with diabetic, while females who carry blood group O less susceptible to infected with diabetes mellitus type II.

يعد مرض السكري من الامراض المعقدة التي يرافقها اضطراب ايضي ويحدث بسبب تداخل العوامل الوراثية والبيئية المرتبطةبالالتهاب وتتطور اعراض المرض تدريجيا ويرتبط بزيادة العوامل الالتهابية لبطانة الاوعية وهي (جزئ الالتصاق بين الخلايا ICAM-1, جزئ الالتصاق بالاوعية الدموية VCAM-1 و E-selectin )والتي يتم عرضها على سطح خلايا بطانة الاوعية الدموية لتساعد الخلايا البيضاء على الاتصاق بالبطانة. وقد حاول العديد من الباحثين ايجاد علاقة معنوية بين الطراز المظهري لمجاميع الدمABO والاستعداد للمرض وتوجد بحوث تؤكد وجود علاقة قوية بين المرض ومجاميع الدم والاضطراب الوظيفي لهذه العوامل يؤدي الى رفع معدل الاصابة بالسكري لدى النساء ولهذا السبب تم تصميم الدراسة الحالية لدراسة العلاقة بين العوامل الالتهابية ومجاميع الدم والاصابة بالسكري لدى النساء العراقيات العربيات, وشملت الدراسة 60 مريضة بالسكري من النوع الثاني مع 20 امراة كمجموعة ضابطة مطابقة للمرضى بالعمر والجنس والعرق, ثم قسمت كلا المجموعتين الى اربع مجاميع ثانوية حسب اصناف الدم واظهرت النتائج فروق معنوية بين المرضى والسيطرة, اذ سجل ارتفاع كل من ICAM-1 وE-selectin وانخفاض VCAM-1 مما يجعل كل من ICAM-1 وE-selectin من العوامل التنبوءية التي تستخدم لتشخيص تطور المرض.وبنفس الوقت اظهرت النساء الحاملات لفصائل الدم A,B,AB ارتباطا موجبا بالسكري بينما النساء الحا ملات لفصيلة الدم O استعدادا اقل للاصابة بالسكري من النوع الثاني.


Article
Immunohistochemical expression of ICAM-1 and Cortactin as cell adhesive molecule and invasive markers in Hodgkin’s and non-Hodgkin’s lymphoma of the head and neck region (A comparative study)

Authors: Zaydoon Mahmood Kasim زيدون محمود قاسم --- Wasan H. Younis وسن حمدي يونس
Journal: Journal of baghdad college of dentistry مجلة كلية طب الاسنان بغداد ISSN: 16800087 Year: 2014 Volume: 26 Issue: 2 Pages: 103-110
Publisher: Baghdad University جامعة بغداد

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Abstract

Background: Lymphomas are a group of diseases caused by malignant lymphocytes that accumulate in lymphnodes and cause the characteristic clinical features of lymphadenopathy. Intercellular adhesion molecule-1 (ICAM-1) (CD54) is a transmembrane glycoprotein belonging to the immunoglobulin superfamily of adhesion molecules.Cortactin was first identified as one of the major substrates for src kinase. because it localized to Cortical actinstructures, The aims of this study was to evaluate and compare the immunohistochemical of ICAM-1 expression ascell adhesion molecule marker and Cortactin expression as invasive marker.Material and Methods: This study was performed on (68) formalin-fixed, paraffin-embedded blocks,histopathologically diagnosed as lymphoma (head and neck lesions).Immunohistochemical staining of ICAM-1andCortactin was performed on each case of the study sample.Results: The expression of ICAM-1 was membranous and cytoplasmic, the study cases showed a 98.5% positivereaction to ICAM-1, score 2 was the most common and found in 69.1% of all cases.The expression of Cortactin wascytoplasmic, 98.5% of cases expressed positive reactions to Cortactin, score 1 was the most common and found in42.7% of all cases.Conclusion: Although the two markers showed a higher expression rate in all lymphomas (both HL and NHL) in thisstudy, they can't be used to differentiate between them, nor can be used to differentiate between the subtypes ofboth HL and NHL. The high ICAM-1positive expression clarified that in addition to its role in cell-cell and cell-stromalinteractions, it participates in proliferation, differentiation and invasion of malignant lymphoma cells.The presentstudy is the first one that used Cortactin as an invasive marker for lymphoma.


Article
THE ROLE OF TUMOR NECROSIS FACTOR Α (TNF-Α) AND INTRACELLULAR ADHESION MOLECULES-1 (ICAM-1) IN ATHEROSCLEROTIC CORONARY HEART DISEASE

Authors: Wurood A.S. Kadhum ورود عبد السلام كاظم --- Nidhal M. Abdul-Muhaymen نضال عبد المهيمن --- Qudus W. Jamal قدس وميض جلال
Journal: IRAQI JOURNAL OF MEDICAL SCIENCES المجلة العراقية للعلوم الطبية ISSN: P16816579,E22244719 Year: 2015 Volume: 13 Issue: 2 Pages: 173-177
Publisher: Al-Nahrain University جامعة النهرين

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Abstract

Background:Tumor necrosis factor-α elaborated soon after myocardial ischemic injury. The intracellular adhesion molecule-1 is required for polymorphonuclear emigration, the primary cause of inflammatory tissue damage due to ischemia-reperfusion.Objective:Detect the serum level of tumor necrosis factor-α and to look for the percentage of expression of intracellular adhesion molecule-1 in atherosclerotic coronary heart disease.Methods:Fifty patients (40 males and 10 females) were enrolled in this study with age range (42-80) years, and fifteen, age and sex matched, apparently healthy individuals. The patients group was further classified into acute and chronic cases. Blood sample was taken from each subject and divided into 2 parts. One part used for lymphocyte separation by using immunocytochmistry to detect intracellular adhesion molecule-1 and the other one for serum separation by using ELISA technique to detect tumor necrosis alpha-α.Results:Significant difference in the concentrations of tumor necrosis factor-α was found between patients and control groups and it was elevated in acute cases compared to chronic cases. Similarly, intracellular adhesion molecule-1 was elevated in patients compared o control groups and more in acute than chronic cases.Conclusions:TNF-α is an important marker that acts on coronary arteries which may contribute to the development of congestive heart disease. Elevation of intracellular adhesion molecule-1 level correlates well with the development of acute events in the disease.Keywords:Atherosclerotic coronary heart disease, TNF-α, ICAM-1, ELISA, immunocytochemistry technique.


Article
INDUCTION OF ICAM-1 AND ICAM-3 IN WOMEN WITH RECURRENT PREGNANCY LOSS
تحفيز الجزيئه اللاصقه بين الخلايا-1 و الجزيئه اللاصقه بين الخلايا -3 في النسوه المصابين بالاجهاض المتكرر

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Recurrent pregnancy loss (RPL) has been found to be associated with increase in the pro-inflammatory cytokines which cause up-regulation of inflammatory mediators including cell adhesion molecules (CAMs) that might act in aggravation of this pathological process.Objective: To find out whether there is a relation between the pathology of RPL and the expression of intracellular adhesion molecule-1 (ICAM-1) and ICAM-3 at the feto-maternal interface in these patients.Methods: Immunohistochemistry technique was performed to detect and determine the expression of ICAM-1 and ICAM-3 using paraffin embedded sections of curate samples obtained from 40 women, who where divided into three groups: 24 women with RPL, 10 women with abortion for the first time, and 6 women with induced abortion.Results: The levels of the expression of both endothelial ICAM-1 and leukocytes ICAM-3 at the feto-maternal interface were found to be significantly up-regulated in the first group as compared with the second and the third groups (p=0.001), with a highly significant positive correlation between these two parameters (r=0.927, p<0.01).Conclusion: ICAM-1 and ICAM-3 might play an important role in the pathology of RPL by increase adherence and recruitment of inflammatory cells at the feto-maternal interface ending with a pregnancy failure

خلفية الدراسة: تتميز حالات فقدان الحمل المتكررة بارتفاع نسبة المدورات المناعية الالتهابية والتي بدورها تعمل على تحفيز العديد من الجزيئات الالتهابيية مثل جزيئات التلاصق الخلوية التي قد يكون لها دور مهم في مرضية فقدان الحمل المتكرر.هدف الدراسة: ايجاد العلاقة بين مرضية فقدان الحمل المتكرر والتعبير الموضعي لجزيئات التلاصق الخلوية ICAM-1 and ICAM-3 .طريقة العمل: استخدمت تقنية التلوين الكميائي النسيجي المناعي لجزيئات التلاصق ICAM-1 و ICAM-3 في عينات الجرف الرحمي والتي تم الحصول عليها من 40 امراة تم تقسيمهن الى ثلاثة مجاميع: 24 امراة حصل لها فقدان حمل متكرر، 10 نساء حصل لهن اجهاض تلقائي للمرة الأولى، و ستة نساء أجري لهن عملية انهاء حمل علاجي.النتائــج: كانت مستويات التعبير الموضعي لجزيئات التلاصق ICAM-1 و ICAM-3 في حالات فقدان الحمل المتكرر ذات زيادة ملحوظة مقارنة مع المجموعتين الثانية والثالثة (p=0.001). فضلاً عن وجود ارتباط معنوي ايجابي كبير بين التعبير الموضعي لهذه الجزيئات (r=0.927, p<0.01).الاستنتاج: قد تؤدي جزيئات التلاصق ICAM-1 و ICAM-3 دورا مهما في مرضية فقدان الحمل المتكرر من خلال زيادة التصاق وجذب الخلايا الالتهابية مؤدية الى فشل الحمل.

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