research centers


Search results: Found 15

Listing 1 - 10 of 15 << page
of 2
>>
Sort by

Article
Rapidly growing non-tuberculosis mycobacterial (NTM) in sputum samples of Iraqi T.B. patients in TB reference lab. in Baghdad
المتفطرات سريعة النمو في قشع المرضى العراقيين المصابين بالسل الرئوي في المختبر المرجعي للتدرن في بغداد

Authors: Dhuha. S. Saleh ضحى سعد صالح --- Hind. Hamed1 هند حامد شاكر
Journal: Iraqi Journal of Science المجلة العراقية للعلوم ISSN: 00672904/23121637 Year: 2013 Volume: 54 Issue: 5 Pages: 1044-1049
Publisher: Baghdad University جامعة بغداد

Loading...
Loading...
Abstract

Non tuberculous mycobacteria (NTM) present in the environment, mainly in water and not transmitted from human to human. The lung is the most common target of NTM infections in human. The symptoms of NTM infection are: fever, weight loss and abdominal pain. 114 sputum samples were examined microscopically using Ziehl-Nelsen stain, all were positive. They cultured on Lowenstein-Jensen media.The results of this study indicate presence of 3 (2.6%) of rapidly growing mycobacteria, phenotypically they resemble T.B. bacilli. They were diagnosed by biochemical tests, they were positive for catalase and pyruvate, negative for niacin, nitrate reduction test, sensitive for Thiophene-2-carboxyl acid hydrozide test& resistant for Para nitro Benzoic acid test. Drug susceptibility test showed that two of these isolates were resistant to isoniazid and rifampicin (MDR/multidrug resistance) while one isolate sensitive to all drugs. This study is a part of a subject aiming to estimate MDR-TB in Iraqi patient.

المتفطرات سريعة النمو موجوده في البيئه وخصوصا في الماء ولا تنتقل من شخص الى اخر. المتفطرات سريعه النمو تصيب الرئتين واعراض الاصابه: الحمى، نقص في الوزن والم في البطن. نتائج الفحص المجهري المباشر باستخدام صبغة زيل نيلسن كانت ايجابيه في 114 نموذجا من القشع ، تم زرع جميع العينات الموجبه لفحص القشع المباشر على الوسط الزرعي الصلب لوفنشتاين جنسن ، وبينت النتائج وجود 3 عزلات وبنسبة 2.6% من المتفطرات سريعة النمو. اخضعت العزلات الثلاث للفحوصات البايوكيميائية حيث اظهرت النتائج انها موجبه لفحص الكاتالايز والبايروفيت ، سالبه لفحص النياسين واختزال النترات ، حساسة لفحص TCH ومقاومه لفحص PNB. تم اختبار حساسية هذه العزلات لمضادات الخط الدوائي الاول واظهرت النتائج ان 2 من هذه العزلات كانت متعددة المقاومة الدوائية (MDR) من خلال مقاومتها للدوائين الايزونيازيد والرفامبسين بينما كانت العزلة الثالثة حساسة لجميع

Keywords

NTM --- MDR.


Article
8.EXTRACTION, PURIFICATION AND THERAPEUTIC USE OF BACTERIOPHAGE ENDOLYSIN AGAINST MULTI-DRUG RESISTANT PSEUDOMONAS AERUGINOSA: AN IN-VITRO AND IN-VIVO STUDY

Authors: Mohammed R. Ali محمد رزاق علي --- Shurooq R. Kadhim شروق ريس كاظم --- Ahmed S. Abdulamir احمد صاحب عبد الامير
Journal: IRAQI JOURNAL OF MEDICAL SCIENCES المجلة العراقية للعلوم الطبية ISSN: P16816579,E22244719 Year: 2018 Volume: 16 Issue: 4 Pages: 405-412
Publisher: Al-Nahrain University جامعة النهرين

Loading...
Loading...
Abstract

Background: Persistent infection with multidrug resistant Pseudomonas aeruginosa (P. aeruginosa) represents a real problem for health care providers. Bacteriophage lytic enzymes or lysins are highly evolved molecules that have been specifically developed by phages to quickly and efficiently allow their progeny to be released from the host bacterium while destructing that bacterium.Objective: Isolation of endolysin from P. aeruginosa bacteriophages, and administering them systematically in vivo lab animal and measuring their therapeutic efficacy as well as evaluation of their biosafety.Methods: This study was performed from March 2015 – August 2017, during which 50 bacteriological samples of P. aeruginosa were collected, and examined for their antibiogram, then bacteriophage cocktails were done for 5 resistant strains of them. Endolysins were extracted from their corresponding bacteriophages and characterized. The enzymatic and antibacterial activities as well in vivo therapeutic efficiency of these enzymes were investigated.Results: This study showed that the extracted endolysin from these bacteriophages was effective in treating laboratory mice from bacteremia with P. aeruginosa and saving their lives when injected intraperitoneal.Conclusion: Endolysin can be extracted directly from their bacteriophages and used effectively in proper doses to treat bacteremia in mice.Keywords: Pseudomonas aeruginosa, bacteriophage, endolysin, MDRCitation: Ali MR, Kadhim SR, Abdulamir AS. Extraction, purification and therapeutic use of bacteriophage endolysin against multi-drug resistant Pseudomonas aeruginosa: An in-vitro and in-vivo study. Iraqi JMS. 2018; 16(4): 405-412. doi: 10.22578/IJMS.16.4.8


Article
Resistance of anti-tuberculosis drugs among pulmonary tuberculosis patients in Yemen
مقاومة الأدوية المضادة للسل بين مرضى السل الرئوي في اليمن

Author: Ahmed M. Al-Haddad أحمد محمد الحداد
Journal: Journal of the Faculty of Medicine مجلة كلية الطب ISSN: 00419419 Year: 2013 Volume: 55 Issue: 3 Pages: 250-253
Publisher: Baghdad University جامعة بغداد

Loading...
Loading...
Abstract

Background: Tuberculosis (TB) is still a major global public health problem worldwide. The global prevalence of Mycobacterium (M tuberculosis) infection has been estimated in 32% of the world population with more than 8 million new cases diagnosed each year.Objective: To estimate drug resistance among previously treated tuberculosis patients, focusing on multi-drug resistant strains at two time intervals (2002 and 2009) in Yemen.Materials and Methods: A total of 192 M tuberculosis complex isolates were collected from patients with positive sputum smear who had been treated previously with the four main anti- tuberculosis drugs for more than two months. The isolates were identified by their colonial morphology, pigmentation, shapes on Ziehl-Neelsen smears, growth on Löwenstein-Jenson medium and biochemical tests as niacin and nitrate tests. A proportional method was used for the in vitro drug susceptibility testing.Results: Of the 192 M tuberculosis complex tested isolates, 55 (28.7%) were resistant to one or more drug; 20 (10.4%) were resistant to one drug, 13 (6.8%) to two drugs, 13 (6.8%) to three drugs and 9 (4.7%) to four drugs. Regarding the resistance to an individual drug, out of 192 tested isolates, 36 (18.7%) were resistant to rifampicin, 34 (17.7%) to isoniazid, 33 (17.2%) to ethambutol and 18 (9.4%) to streptomycin and these results were without a statistical significance. The incidence of multidrug resistance against rifampicin and isoniazid with or without other drugs was 13.5% among the tested M tuberculosis complex strains and this result was also without a statistical significance. Conclusion: The results of this study revealed nearly similar drug resistance patterns for the tested isolates in comparison with previous findings of 2002 and the emergence of more multi-drug resistance M tuberculosis complex strains after a time interval in Yemen.Key words: Tuberculosis, resistance, anti-tuberculosis drugs, MDR, Yemen.

لا يزال خمج السل الرئوي يشكل مشكلة صحية رئيسية على مستوى العالم، حيث تم تحديد مدى انتشاره بين سكان العالم بنسبة 32% مع زيادة 8 مليون من الحالات المشخصة الجديدة كل عام. هدف الدراسة: تحديد مدى مقاومة الأدوية المضادة للسل الرئوي بين مرضى معالجين سابقا وكذا التركيز على ذريات المتفطرة السلية المعندة على عديد من الأدوية في فترتين زمنيتين (2002 و 2009) باليمن .طريقة الدراسة: شملت هذه الدراسة على 192 عزلة للمتفطرة السلية أخذت من مرضى كان فحص القشع لديهم إيجابيا، و ذلك بعد فترة علاج تزيد عن شهرين بالأدوية الأربعة المضادة للسل (أيزونياسيد, ريفامبيسين, ستربتوميسين, ايثامبوتول). بعد تعريف هذه العزلات بواسطة الفحوصات التأكيدية من حيث الشكل المميز للمتفطرة واللون والنمو على الوسط الزراعي والتفاعلات الحيوية، تم اجراء فحص الحساسية في الزجاج للأدوية الأربعة المذكورة أعلاه مع كل عزلة على حده.النتائج: تم تحديد 55 عزلة (28.7%) من اجمالي العزلات 192 المدروسة وكان توزيع العزلات المقاومة لدواء واحد أو أكثر كالتالي: 20 عزلة مقاومة لدواء واحد و13 لدوائين و13 لثلاثة أدوية وأخيرا 9 لأربعة أدوية. نتيجة مقاومة هذه العزلات لدواء واحد أو أكثر لم تكن ذات أهمية إحصائية. ما يخص مقاومة العزلات لكل دواء على حده فكانت النتيجة كالتالي: 36 عزلة مقاومة لدواء ريفامبيسين و34 عزلة لدواء أيزونياسيد و33 عزلة لدواء ستربتوميسين و18 عزلة لدواء ايثامبوتول من اجمالي 192 عزلة. و لم تكن أيضا هذه النتائج ذات أهمية إحصائية. أما ما يخص مقاومة هذه العزلات على عديد من الأدوية فكانت هناك 26 عزلة مقاومة من اجمالي العزلات 192 المدروسة بنسبة 13.5% ولم تكن أيضا هذه النتيجة ذات أهمية إحصائية.الاستنتاج : اظهرت هذه الدراسة عن وجود نسبة أقل قليلا من العزلات المعندة للأدوية المضادة للسل سواء كان لدواء واحد أو أكثر وظهور ذريات أكثر من المتفطرة السلية المعندة على عديد من الأدوية بعد فترة من الزمن في اليمن عند مقارنة نتائج الدراسة الحالية بنتائج دراسة العام 2002.


Article
RISK FACTORS FOR MULTI-DRUG RESISTANT TUBERCULOSIS: A REVIEW
عوامل الخطر لمقاومع مرض السل لادوية المتعدد - استعراض

Authors: MUAYAD A. MERZA مؤيد اغالي ميرزا --- AHMAD M. SALIH احمد محمدصالح
Journal: Duhok Medical Journal مجلة دهوك الطبية ISSN: ISSN: 20717334 (online)/ ISSN: 20717326 (Print) Year: 2010 Volume: 4 Issue: 2 Pages: 1-7
Publisher: University of Dohuk جامعة دهوك

Loading...
Loading...
Abstract

The current review aims to determine the risk factors associated with multi-drugs resistant TB (MDR-TB). Previous treatment is the most important risk factor for inducing MDR-TB.Other important associated factors are: immigration, age 45-64 year, male sex, HIV infection, alcoholism, smoking, diabetes mellitus, and poor socio-economic factors. Effective treatment, control and prevention of emergence and transmission of drug-resistant TB are required in all countries. To achieve this, the World Health Organization (WHO) recommended the adoption of Directly Observed Therapy Short-Course (DOTS) programme which involves giving effective and regular anti-TB drug supply, government security and financing commitment, case detection and diagnosis by smear microscopy, and monitoring the performance and outcome. It is highly recommended to strictly follow the appropriate WHO treatment guidelines, to ensure adequate success rate of treatment in drug-susceptible and drug-resistant strains; this will limit emergence of resistant strains and prevent spread of the disease. The emergence of aggressive new forms of drug-resistant TB is worrying thatrequires reinforcement of control measures. This demands special attention to case detection and prompt treatment of MDR-TB, extensively drug resistant TB (XDR-TB), and totally drug resistant TB (TDR-TB) to prevent transmission of the disease and further development of drug-resistant strains beyond this stage.


Article
Isolation of Mycobacterium tuberculosis and testing their susceptibility to antimicrobial agents by using Bactec 960.

Loading...
Loading...
Abstract

Abstract:This study was done from 3/7/2011 to 1/1/2012 , different clinical samples were collectedfrom (1235) patients that coming to the Specialized center for chest and respiratory diseases /Baghdad to investigate Mycobacterium tuberculosis and study their resistant to first and secondline antimicrobial agents by using Bactec 960.The results obtained from direct smear examination with Ziehl – Neelsen stain in (294)patients that indicated these patients are Infected with tuberculosis .These samples has beencultured on solid medium Lowenstein Jensen media (L.J.) and liquid medium ( middle brook 7H8broth base) in the Mycobacteria Growth Indicator Tubes (MGIT) using a device (BACTECMGIT 960 System).Results of (275) samples that represented (22.27%) from total samples wereshowed a significant real growth that belong to bacteria Mycobacterium tuberculosis.From (275) isolates tested to 1st line anti TB (streptomycin (SM), isoniazid (INH),rifampin (RMP) and ethambutol (EMB)), (196) isolates were resistant to the first lineantimicrobial agents that used in the treatment of tuberculosis by using Bactec 960. (125) isolates(63.77%) showed resistance to multiple antimicrobial drugs (MDR-TB).Furthermore, the sensitivity test was done on thirty isolates that characterized by multipledrug resistance criteria (MDR-TB) on second line antimicrobial agents to investigate ExtensivelyDrug-Resistant Tuberculosis(XDR-TB) between these isolates. The results showed that the bestantibiotics in their impact on the isolates (MDR-TB) is (PAS) P-aminosalicylic acid as thepercentage of resistance was (3.3%).

الخلاصة:جمعت 1235 عینة سریریة مختلفة من المرضى الوافدین الى معھد الامراض الصدریة والتنفسیة / بغداد تراوحت2012 لعزل بكتریا -1 - 2011 لغایة 1 -7 - اعمارھم بین ( 4 أشھر الى 70 سنة) من كلا الجنسین للفترة من 3.Bactec ودراسة حساسیتھا لمضادات التدرن الخط الاول والثاني بأستعمال تقنیة 960 Mycobacterium tuberculosisأظھرت نتائج الفحص المباشر للمسحات المصبوغة بصبغة زیل – نیلسون ان ( 294 ) مریضاً یعاني من مرضModified والوسط الزرعي السائل Lowenstein Jensen media (L.J) التدرن، زرعت العینات على الوسط الصلبباستعمال (MGIT) Mycobacteria Growth indicator Tube في انابیب خاصة middlebrook 7 H8 broth baseوقد اظھرت النتائج ان ( 275 ) عینة ( 22.27 %) من المجموع الكلي للعینات (BACTEC MGIT 960 System) منظومة. Mycobacterium tuberculosis نموا حًقیقیا وتبین انھا تعود لبكتریاأختبرت حساسیة ھذه العزلات لمضادات التدرن الخط الاول (الایزونیازید والستربتومایسین والاثیمبتولوالریفامبسین) باستعمال تقنیة الباكتیك 960 ، وأظھرت النتائج ان ( 196 ) عزلة من اصل ( 275 ) كانت مقاومة لمضادات.(MDR-TB) التدرن. من جانب اخر كانت ( 125 ) عزلة من اصل ( 196 ) بنسبة (% 63.77 ) مقاومة متعددة للمضادات


Article
Detection of MDR Gene (IFITM3) and P- glycoprotein Expression in Patientswith Hodgkin's Lymphoma in AL-Ramadi Teaching Hospital

Author: Akram S. Baraa1, NorryaA.Ali1, Abdul– RahmanM.2,Ahmed Abdul–Jabbar3
Journal: Iraqi Journal of Biotechnology المجلة العراقية للتقانات الحياتية ISSN: 18154794 Year: 2016 Volume: 15 Issue: 2 Pages: 30-41
Publisher: Baghdad University جامعة بغداد

Loading...
Loading...
Abstract

The present study aimed to shed light on the follow up of Hodgkin's lymphoma patients at initial diagnosis and after treatment, to assess the response and early relapse after chemotherapy through evaluating the gene expression level of one of the major multidrug resistance genes which is the multidrug resistance 1 (MDR1),and investigate the presence of P-glycoprotein by immunohistochemistry assay. The study was conducted on fifty Hodgkin's lymphoma patients during the period extendedfrom January 2015 to November 2015.The patients included 41(82%) males and 9(18%) females ,with ages ranged from 12 to 81 years. The study also included ten persons (7 males and 3 females ) as healthy control group . All patients were followedup by doing some laboratory test such as complete blood count before and after chemotherapy, and the tumor markers which indicate the response degree of patients to chemotherapy DNA was extracted from paraffin embedded tissue, which were collected from AL-Ramadi teaching Hospital / Histopathology unit, in order to detect the Interferon-induced trans membrane protein IFITM3 (MDR) gene using PCR technique .The results showed in the healthy individuals, there were no mutation presence in the MDR gene in patients and control. Results of immunohistochemistrystudy showed thatthe presence of (MDR1) P glycoprotein had played a great role in regulation of the cancer cells growth. Depending on theintensity of coloration, and percent of cancer cells stained , the results indicated the production of P-glycoprotein in 15(30%) case as 1+, 6(12%) case as 2+ and 10(20%)case as 3+, while other cases 19 (38%) were negative.


Article
MOLECULAR DETECTION OF MULTIDRUG RESISTANT ACINETOBACTER BAUMANNII FROM DIFFERENT CLINICAL SAMPLES

Loading...
Loading...
Abstract

Background:Acinetobacter baumannii (A. baumanii) has recently emerged as a major pathogen causing nosocomial infections in patients admitted to intensive care units with a surprisingly rapid acquisition of antibiotic resistance. Objective:To study the rate of occurrence of A. baumanii isolated from different clinical specimens and to study the rate of occurrence of multidrug resistance especially NDM-1 and qnrA genes.Methods:A total of sixty-two (62) clinical isolates of A. baumanii were tested against 14 antibiotics by disc diffusion method. Minimum inhibitory concentration was determined by agar dilution method for resistant isolates. Polymerase chain reaction (PCR) was performed to detect bla NDM-1 and qnrA genes.Results:All Acinetobacter isolates were complete resistant to Colistin and Tigacycline (100%), while high rate of resistance was to Aztronem (93.54%), Cefotaxime, (91.93%), Ceftriaxone (88.70%) and Meropinem (80.64%). Moderate - to- low rate of resistance was to Ceftazidime (77.41%), Cefepim (75.80%), Peperacillin and Ciprofloxacin (74.19%), Gentamicin (69.35%), Levofloxacin (64.51%), Amikacin (61.29%) and Impenim (50%). The highest minimum inhibitory concentration value 128 μg/mL was to Cefotaxime, Tigacycline and Colistin. While the lowest value 8 μg/mL was to Gentamicin, Imipenem, Ciprofloxacin and Levofloxacin. The PCR results showed that 50% of Metallo beta lactamase producers A. baumannii was carried bla NDM-1 gene in chromsomal DNA and 24 (48%) of Flouroquinolone resistance A. baumannii harbored qnrA gene in chromsomal DNA while the prevalence of qnrA gene was (60.6%) in plasmid DNA.Conclusion:There is a high prevalence of multidrug resistant A. baumannii in different samples from Baghdad with the high prevalence of bla NDM-1 and qnrA genes among this bacterium.Keywords:Acinetobacter baumannii, bla NDM-1, qnrA- gene, MDRCitation:Mshachal MA, Abdulrahman TR, Khudair MS, Hassan JS. Molecular detection of multidrug resistant Acinetobacter baumannii from different clinical samples. Iraqi JMS. 2017; Vol. 15(3): 314-323. doi: 10.22578/IJMS.15.3.14


Article
Molecular detection of multidrug-resistant mycobacterium tuberculosis in Al Muthanna province

Author: Haider H. Mitab
Journal: Muthanna Medical Journal مجلة المثنى الطبية ISSN: 2226146x Year: 2018 Volume: 5 Issue: 1 Pages: 1-11
Publisher: Al-Muthanna University جامعة المثنى

Loading...
Loading...
Abstract

Multidrug-resistant tuberculosis (MDR-TB) is a global problem that many countries are challenged with. Rapid and accurate detection of MDR-TB is critical for appropriate treatment and controlling of TB. The aims is detection of multidrug-resistant Tuberculosis from cultured samples by using Polymerase Chain Reaction. A total of 30 M. tuberculosis isolates from cases with diagnosed TB by GeneXpert, AFB and Culture on L. J media after incubation period from 3-8 weeks, DNA extraction from bacteria colonies. Resistant isolates were tested for characterization of mutations in the rpoB, KatG InhA1 and IhA2 genes by Real Time PCR. The results of the real time PCR showed that mutations of genes (rpoB, katG, inhA1 and inhA2) that were responsible for resistance to rifampicin and isoniazid. The test showed positive results for resistance genes (20%, 10%, 6.6%, 10% Respectively) as well as note that the values ​​of Ct for this test ranged from (12-38.25), and the melting points of the genes were between (85-88.5 Co). Real time PCR results identified three mutations of MDR (rifampicin and isoniazide) resistance genes, whereas there was one MDR mutation of molecular diagnostic results with the GeneXpertMTB/RIF test for rifampicin. When comparing the results of the Real time PCR and GeneXpert tests at the level of the genetic mutation with rifampicin, the real time PCR test showed four resistance mutations for the rpoB gene for both new cases and relapse tuberculosis as well as one rpoB mutant for under treatment patient. Both molecular tests have agreed to identify one rpoB mutant in the case of failure TB treatment.


Article
Health-related quality of life of tuberculosis Patients in Bagdad and Socio-demographic Factors Associated with Multiple Drug Resistant Tuberculosis (MDR TB)
نوعية حياة مرضى التدرن في بغداد وعلاقة بعض العوامل الاجتماعية والديموغرافية في الاصابة بالتدرن المقاوم

Author: Ahmed Abed Marzook احمد عبد مرزوك
Journal: Diyala Journal of Medicine مجلة ديالى الطبية ISSN: 97642219 Year: 2016 Volume: 10 Issue: 1 Pages: 9-15
Publisher: Diyala University جامعة ديالى

Loading...
Loading...
Abstract

Background: Tuberculosis is a highly infectious disease that primarily affects the lungs. It can also affect other organs. TB was once called “consumption.” It is the world’s second-most fatal infectious agent, after HIV/AIDSObjective: To evaluate the effect of sodium nitrate, vitamin E and vitamin C administration on liver enzymes, alanine transaminase, aspartate transaminase and alkaline phosphatase. This research aimed to study the effect of tuberculosis (TB) on quality (QOl) of life of MDR and non DR TB patients, it also to explore any possible association between getting MDR TB and socio- demographic variablesMaterials and Methods: A cross-sectional study was conducted in Chest and Respiratory Diseases Center (CRDC) –Baghdad during 2015. The World Health Organizations Quality of Life Assessment (WHOHQL-BREF) was used to asses QOL of 50 MDR and 50 Non DR sputum positive pulmonary TB patients. Socio-demographic variables were studied. SPSS V. 22, was used in statistical analysis, Chi-square and Mann-Whitney-U tests were used to test significance of differences, PV≤ 0.05 was considered significant.Results: The mean age was 43.46 ± 14.8 and 37 ± 16.16 for MDR-TB and non-DR-TB respectively. The age difference was significant (p=0.048). Males constitute a majority in both groups (DR-TB 64% and non-DR-TB 59%). Smoking was higher in DR-TB than non-DR-TB (57% and 38% respectively) and difference was significant (P =0 .016). Significant differences between DR-TB and non-DR-TB in global QOL, global health, physical, psychological, social and environmental domains (P=0.004, 0.014, 0.001, .0019, 0.001 and 0.001 respectively).Conclusion: Patients with MDR-TB had lower mean scores than non-DR-TB for overall HRQOL domains. There is association between getting MDR TB and being older in age, lower educational level, smokers and being jobless.

خلفية الدراسة: داء السل (التدرن ) يعد من الامراض الخطيرة التي تؤثر بدرجة اساسية في الرئة ويعد من المسبات المؤدية للموت بعد مرض النقص المناعي.اهداف الدراسة: دراسة تاثير التدرن والتدرن المقاوم للادوية على نوعية حياة المرضى وعلاقة بعض العوامل الاجتماعية والديموغرافية بالاصابة بالتدرن المقاوم.المرضى والطرائق : دراسة مقطعية مقارنة اجريت في مركز الامراض الصدرية في بغداد عام 2015 شملت 50 مريضا مصابا بالتدرن الرئوي المقاوم و50 مريضا غير مقاوم وتم قياس نوعية حياة المرضى باستخدام مقياس نوعية الحياة المختصر لمنظمة الصحة العالمية مع قياس العلاقة بين بعض العوامل الاجتماعية والديموغرافية وحصول التدرن المقاوم .تم استخدام اختبار كاي ومان وتني لقياس الفروق بين المجاميع مع اعتبار pv≤0.05 قيمة دالة احصائيا.النتائج: كان متوسط العمر للحالات المقاومة للعلاج وغير المقاومة هو 43.46±14.8 و 37.36 ±16.15 على التوالي (pv=0.043) وقد شكل الرجال اغلبية في المجموعتين 66% و 59% مقارنة بغير المقاومين للعلاج وتبين ان التدخين كان اكثر انتشارا بين المقاومين للعلاج 57% مقابل 39% (pv=0.016) وكان مستوى التعليم والعمل افضل لدى غير المقاومين وبفارق معنوي دال احصائيا (pv=0.045).كما بينت الدراسة ان نوعية حياة المرضى كانت اسوأ لدى المرضى المقاومين للعلاج في جميع المجالات مع تأثر مجال البيئة والمجال الاجتماعي اكثر من بقية المجالات وكان مستوى القناعة بالحالة الصحية ونوعية الحياة افضل لدى المرضى غير المقاومين لادوية التدرن وبفارق دال احصائيا.الاستنتاجات: كان المرضى الذين يعانون من السل المقاوم للأدوية أقل متوسطات درجات من غير DR-TB-للنطاقات HRQOL الشاملة. هناك ارتباط بين حدوث السل المقاوم للأدوية و كبار السن في العمر، المستوى التعليمي المنخفض, المدخنين و العاطلين عن العمل


Article
Molecular and Nanotechnical Study for Antibiofilm Formation and CsuE Gene Expression Activities of Synthesized Iron Oxide Nanoparticles Against Multidrug-Resistant Acinetobacter baumannii Isolates

Author: Haider Turky AL-Mousawi1 , Mohammed I. AL-Taee2 , Maarib N. Rasheed2 , Qabas Nima AL-Hajjar3
Journal: Iraqi Journal of Biotechnology المجلة العراقية للتقانات الحياتية ISSN: 18154794 Year: 2019 Volume: 18 Issue: 2 Pages: 201-2015
Publisher: Baghdad University جامعة بغداد

Loading...
Loading...
Abstract

This study aimed to investigate the influence of synthesis of Iron Oxide Nanoparticles (Fe3O4NPs) by co-precipitation method on biofilm formation and CsuE gene expression in multidrug resistance Acinetobacter baumannii (MDRA.b) which represents one of the important causing agents of nosocomial infection. A. baumannii isolates used in current study and isolates from different clinical sources (wounds, burns, urine, sputum, blood and throat) that able to produce strong biofilm. Synthesis and characterisation the physical-chemical nature of Fe3O4 NPs was carried out using UV-visible spectrophotometer, fourier transform infrared (FT-IR), X-ray diffraction (XRD), Atomic force microscope (AFM) and scanning electron microscope (SEM). Results show that they were very fine crystalline sizes reaching to 11±1 nm by XRD ,with a mostly spherical in shape and average of nanoparticles size between (40- 47) nm by SEM and AFM, respectively. Minimum inhibitory concentration (MIC) and sub-MIC test of Fe3O4 NPs at concentrations (15.75 to 2000) μg/ml against A.baumannii isolates had been determined using tube broth method measured by optical densities values at 630 nm. The results of the experiment of the best sub-MIC with concentration of Fe3O4 NPs 125μg/ml showed a significant difference at p˃0.05 the anti-biofilms inhibitory on polystyrene surface of microtiter plates for the bacteria isolate under study. Biofilm formation CsuE gene expression was investigated by using RT-qPCR technique with reference 16SrRNA gene before and after treatment with Fe3O4 NPs. The results showed a significant difference at p˃0.05 in Cycle threshold (Ct) values for CsuE gene expression of the isolates. The study concludes that the synthesized magnetic Fe3O4 NPs with 125µg/ml gave excellent antibiofilm activities and inhibitory efficiency against A.baumannii isolates and the potentiate to down regulate of gene expression fold for biofilm formation encoded by CsuE gene of A.baumannii leading as a result to have low biofilm production.

Listing 1 - 10 of 15 << page
of 2
>>
Sort by
Narrow your search

Resource type

article (15)


Language

English (11)

Arabic and English (3)

Arabic (1)


Year
From To Submit

2019 (1)

2018 (2)

2017 (4)

2016 (3)

2013 (3)

More...