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Article
Differentiation of Mouse Bone-Marrow Mesenchymal Stem Cells into Motor Neuron Cells in vitro

Authors: Rafal Hussamildeen Abdullah --- Shahlla Mahdi Salih --- , Nahi Yosef Yaseen --- Ahmed Majeed Al-Shammari
Journal: Al-Nahrain Journal of Science مجلة النهرين للعلوم ISSN: (print)26635453,(online)26635461 Year: 2016 Volume: 19 Issue: 2 Pages: 111-116
Publisher: Al-Nahrain University جامعة النهرين

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Abstract

Motor neuron cell is responsible for transfer neural instruct from brain to peripheral muscle through spinal cord; therefore any defect in these cells or spinal cord will affect motion. This study was designed to induce differentiation of Bone marrow mesenchymal stem cells (BM-MSCs) into neuron cells. BM-MSCs were isolated from bone stroma of femur and tibia of albino male mice and tested immunocytochemically for CD44, CD90, and CD105 expression and showed positive staining, while showed negative staining for CD34. Differentiation of BM-MSCs to motor neuron involved two main steps. Firs; induction of BM-MSCs by addition of 1mM mercaptoethanol (BME) in fetal bovine serum (FBS) in minimum essential medium MEM for 24 h and 2mM BME in free serum media for 2h. In the second step of induction retinoic acid, sonic hedgehog and nerve growth factor were added in free serum MEM for 4 days. Results revealed that the differentiation medium used was very efficient in directing the BM-MSCs to motor neural cell and showed positive reactivity to specific motor neural markers that used for detection of motor neuron cells like microtubule associated protein-2 antibodies and acetylcholine transferase antibody.

الخلايا العصبية الحركية مسؤولة عن نقل الايعاز العصبيمن المخ الى الاطراف العضلية من خلال الحبل الشوكي لذلك اي خلل فيها او في الحبل الشوكي يؤدي الى خلل في الحركة.صممت الدراسة لتحفيز تمايز الخلايا الجذعية الوسيطة الى خلايا حركية عصبية. اختبرت الخلايا الجذعية الوسطية الماخوذه من نخاع العظم والمعزولة من نسيج الستروما لعظم الفخذ و الساق لذكر فئران الالبينو واختبرت بالتفاعل المناعي الكيميائي الخلوي للمستقبلاتCD44,CD90,CD105 وسلبي لمستقبل CD34.ان تمايز الخلايا الجذعية من نخاع العظم الى خلايا عصبية حركية تتضمن خطوتين. الاولى: خطوة قبل التمايز التي تتضمن اضافة 1 ملي مول من مادة البيتا مركلتوايثانول في سيرم جنين العجل في وسطMEM لمدة 24 ساعة و في تركيز 2 مليمول بيتامركبتوايثانول في وسك زرعي خالي من السيرم لمدة 2 ساعة.الخطوة الثانية تسمي خطوة التحفيز وتتضمن اضافة1 مايكرومولاري من الريتنوك اسيد و من السونك هيدجهوك و من نيرف كروث فاكتر في وسطMEM لمدة 4 ايام واظهرت النتائج ان وسط التمايز المستخدم فعال جدا في تحويل الخلايا الجذعية الوسطية الى خلايا عصبية حركية واضهرت التفاعلات المناعية الكيمياوية نتائج ايجابية لمعلامات الخلايا الجذعية الحركية بوسطة استخدامCHAT وMAP-2 .


Article
Evaluation the effect of autologous bone marrow – derived mesenchymal stem cells as a treatment in diabetic rabbits

Authors: Mohamed Abdul-Hameed Mohamed محمد عبد الحميد محمد --- Wasan H. Younis وسن يونس --- Nahi Y.Yaseen ناهي ياسين
Journal: Journal of baghdad college of dentistry مجلة كلية طب الاسنان بغداد ISSN: 16800087 Year: 2012 Volume: 24 Issue: special issue 2 Pages: 55-60
Publisher: Baghdad University جامعة بغداد

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Abstract

Back ground: Type 1 diabetes is the result of an autoimmune attack against the insulin-producing beta cells of thepancreas. Current treatment for patients with type 1 diabetes typically involves a rigorous and invasive regimen oftesting blood glucose levels many times a day along with injections of recombinant insulin. Many recent researcheshave shown that stem cell therapy can be the best choice for treatment of this disease. The aims of this researchwere investigating regeneration of pancreatic beta cells of type 1 diabetic rabbits after stem cell transplantation.Materials and Methods: 32 rabbits weighting an average of (2.5 - 3 kg) were used in this experimental study, anddivided into 2 groups as follows; group A ( contains 16 controlled diabetic rabbits received insulin as a treatment )and group B ( contains 16 diabetic rabbits received autologous mesenchymal stem cells as a treatment).Theinduction of diabetes was achieved by a single dose of intravenous injection of the Alloxan, which was administeredto the rabbits via the marginal ear vein, mesenchymal stem cells were differentiated into insulin – producing cells andreimplanted into the rabbits of group B with daily monitoring of blood glucose level and body weight.Results: The insulin – producing cells regulated the hyperglycemia resulted from diabetic rabbits , 7 to 9 days afterreimplantation the blood glucose level were decreased from about( 400 mg/dl into 180 mg/dl).Conclusions: Islet-like functional cells can be differentiated from bone-marrow mesenchymal stem cells (MSCs),which may be a new procedure for clinical diabetes stem -cell therapy, these cells controlled blood glucose level indiabetic rabbits as the effect of insulin. MSCs play an important role in diabetes therapy by islet differentiation andtransplantation.

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