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Article
Immunoflourescent assessment of Herpes Simplex Virus (HSV) type 1 in oral lichen planus

Author: Muthanna K. Ali مثنى علي
Journal: Journal of baghdad college of dentistry مجلة كلية طب الاسنان بغداد ISSN: 16800087 Year: 2014 Volume: 26 Issue: 1 Pages: 103-107
Publisher: Baghdad University جامعة بغداد

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Abstract

Background: Oral lichen planus is one of the most common dermatological diseases presenting in the oral cavity.Hence, viral infection of the oral mucosa may be involved in the pathogenesis of oral lichen planus, Taking in toconsideration the oncogenic potential of HSV-1, this study aimed to assess the presence of Herpes Simplex Virustype one by direct immunoflourescent in oral lichen planus. This study aimed to assess the presence of HSV type1 bydirect immunofluorescent in histopathologically diagnosed OLPMaterial and Method: Twenty formalin fixed embedded tissue blocks of oral lichen planus with 2 Positive control caseswere taken from patients having infection with herpes labialis, US Biological herpes simplex virus-1 Glycoprotein C wasused for detection of HSV-1 Ag by direct immunofluorescence assayResults: One case of oral lichen planus showed positivity of HSV 1 with a non- statistical significance.Conclusion: The present study couldn’t find any correlation between HSV-1 positivity with clinical andhistopathological features of OLP

Keywords

OLP --- HSV --- immunofloresant assay


Article
P16 Protein and Human Papillomavirus (HPV16, 18) Expressions in Oral Lichen Planus and Squamous Cell Carcinoma

Authors: Suhaib Raghib Muhsin صهيب راغب محسن --- Ahlam Hameed Majeed احلام حميد مجيد
Journal: Journal of baghdad college of dentistry مجلة كلية طب الاسنان بغداد ISSN: 16800087 Year: 2015 Volume: 27 Issue: 4 Pages: 96-100
Publisher: Baghdad University جامعة بغداد

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Abstract

Background: Oral carcinogenesis is a molecular and histological multistage process featuring genetic andphenotypic markers for each stage, which involves enhanced function of several oncogenes and/or thedeactivation of tumor suppressor genes, resulting in the loss of cell cycle checkpoints. The progression towardsmalignancy includes sequential histopathological alterations ranging from hyperplasia through dysplasia tocarcinoma in situ and invasive carcinoma. The p16 gene produces p16 protein, which in turn inhibits phosphorylationof retinoblastoma, p16 play a significant role in early carcinogenesis. Human papillomavirus is a well establishedheterogeneous virus and plays an important role in oral cancers. The aims of the study were to evaluate, compareand correlate the immunohistochemical expression of p16 protein and HPV16/18 with each other in oral lichenplanus and oral squamous cell carcinoma, and with various clinicopathological findings.Materials and methods: Forty formalin-fixed, paraffin embedded tissue blocks (24 cases of oral lichen planus, and 16cases of oral squamous cell carcinoma) were included in this study, an immunohistochemical staining wasperformed using anti p16 monoclonal antibody, and anti HPV16/18 monoclonal antibodies.Results: Positive IHC expression of p16 was found in 11 cases (68.75%) of OSCC, and in 19 cases (79.166%) of OLP.Positive IHC expression of HPV16 was found in 2 cases (12.5%) of OSCC, and in 1 case (4.16%) of OLP. IHC expressionof HPV18 showed negative expression in all cases of OSCC, and found only in 1 case (4.16%) of OLP.Conclusions: This study signifies the statistically non significant correlation between p16 and HPV 16/18 in OLP andOSCC

Keywords

OLP --- OSCC --- P16 --- HPV


Article
Immunohistochemical expression of Cyclin D1 and NF-KB p65 in oral lichen planus and oral squamous cell carcinoma (Comparative study)

Authors: Amer Mousa Abid عامر موسى عبد --- Muna S. Merza منى ميرزا
Journal: Journal of baghdad college of dentistry مجلة كلية طب الاسنان بغداد ISSN: 16800087 Year: 2014 Volume: 26 Issue: 1 Pages: 80-87
Publisher: Baghdad University جامعة بغداد

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Abstract

Background: Oral Lichen Planus (OLP) is a chronic inflammatory mucosal disease, presenting in various clinical formsWHO had regarded OLP as a precancerous conditions in 1978 because of its potential with cancer. Both antigenspecificand nonspecific mechanisms involved in the pathogenesis of OLP. Oral Squamous Cell Carcinoma (OSCC) isthe most common malignant neoplasm of the oral cavity representing more than 94% of oral cancer. It occurs indifferent sites and has many etiological factors. Cyclin Dl is a proto-oncogene which consider as the key protein inthe regulation of cell proliferation and its overexpression led to the occurrence and progression of malignanttumors.NF-KB p65 is a member ofNF-kB family of transcription factors that widely used by eukaryotic cells as aregulator of genes that control cell proliferation and cell survival, also plays a major role in inflammation. The aims ofthis study were to evaluate the immunohistochemical expression of Cyclin D1 & NF-KB p65 in OLP & OSCC & tocorrelate the expression of the studied markers with the clinicopathological findings and with each other.Materials and Methods: Fifty (50) formalin – fixed, paraffin – embedded blocks of both Oral Lichen Planus (25 cases) &Oral Squamous Cell Carcinoma (25 cases) were collected pro- and retrospectively were included in this study.Hematoxylin & Eosin stain was performed for each block for reassessment of histopathological examination. Animmunohistochemical staining was performed using anti Cyclin D1 and anti NF-KB p65 monoclonal antibodies.Results: Of twenty five OLPstudied cases , positive Cyclin D1 & NF-KB p65 expression was found in (84%) and (88%)ofthe cases respectively. For OSCC ,out of 25 studied cases ,positive Cyclin D1 & NF-KB p65 expression was observe in(88%) and (96%) of cases respectively. Statistically significant correlation between Cyclin D1immuno- reactivity andclinical presentation of OLP was found. Statistically significant correlation of Cyclin D1 immunoreactivity with tumorgrade andNF-kBp65 immunoreactivity with tumor stage in OSCC cases was found. Statistically a highly significantcorrelation between the expression of two studied markers in OLP and OSCC was found.Conclusion: A highly significant correlation was seen regarding the expression of both markers with each other,suggesting their cooperative role in the pathogenesis of OLP and OSCC

Keywords

OLP --- OSCC --- Cyclin D1 --- NF-KB p65 --- Immunohistochemistry


Article
Estimation of Salivary Levels of Pro Inflammatory Cytokines (Interleukin-1α and Interleukin-8) in Iraqi Lichen Planus patients, the association of these parameters with dental plaques, gingivitis and smoking status

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Abstract

Background: Oral lichen planus (OLP) is chronic inflammatory disease of the oralmucosa whose etiology is still unknown but mounting evidence points to theimmunologic basis of this disorder.The aim of the present study was to assess the level of salivary IL-1α, IL- 8, salivaryflow rate (SFR), the plaque and gingival indices in lichen planus patients, and toassess their level pre and post treatment with topical steroid in study group, inaddition to the assessment of relation of smoking to these parameters in oral lichenplanus patients.Materials and methods: Whole saliva from fifteen (15) patients with oral lichenplanus, eighteen (18) healthy persons, were investigated for the presence of IL-1α, IL-8 by enzyme linked immunosorbent assay ( ELISA). In addition, the flowrate of saliva (SFR) measured in both groups, plaque and gingival indices alsoassessed pre and post treatment.Results: The results showed a significant elevation of pro-inflammatory cytokines IL-1α , IL- 8, plaque and gingival indices in study group in comparison to controlgroup except the SFR where it was higher in control group than study group alsoshowed that some of these parameters significantly related to smoking statues.The studies parameters also showed significant variation after using of topical steroidin which the salivary level of cytokines IL-1 α , IL-8, plaque &Gingival indiceswere significantly decreased after treatment except SFR was significantly increaseafter treatment.Conclusion: Patients with oral lichen planus have had high levels of inflammatorycytokines in their saliva. The results also showed wide variations in levels amongpatients in pre and post treatment with steroid, in addition the level of proinflammatory cytokines significantly related to smoking status. The resultssuggest links between the proinflammatory cytokines ( IL-1 α , IL-8 ) from oneside and oral lichen planus disease, medication used for treatment and smokingstatus of the patients from another side.

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